• 한국수자원학회논문집
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• 제45권10호
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• pp.969-981
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• 2012

본 연구에서는 서로 다른 ENSO(El Ni$\tilde{n}$o Southern Oscillation) 형태에 따른 한강유역의 연최대홍수량과 저유량의 정량적 변화 및 발생시점의 변화를 분석하였다. 경향성 분석결과, 연최대유출의 경우 한강 전체 유역면적의 48.3%가 통계적으로 유의한 증가패턴을 보였으며, 연최대 유출의 발생시기는 한강 서부유역에서 늦어지고, 동부유역에서는 빨라지는 경향이 있음을 확인하였다. 또한 7일 저유량의 경우, 24개 중권역중 6개 유역(전체 면적의 26.0%)에서 통계적으로 유의한 감소경향을 보였으며, 저유량의 발생시기는 한강중상류 유역에서 빨라지는 경향이 있음을 확인하였다. CT(Cold tongue)/WP(Warm-pool) El Ni$\tilde{n}$o 시기에 연최대유출의 발생시점의 차이는 크지 않으나, CT El Ni$\tilde{n}$o 시기에는 전체유역의 89.0%에서 연최대유출량이 평년보다 작게 나타났다. 또한, 7일 저유량의 발생시점은 WP El Ni$\tilde{n}$o 시기에 평균적으로 약 17일 정도 빠르며, CT El Ni$\tilde{n}$o (WP El Ni$\tilde{n}$o)시기에는 전체 유역의 72.7%(20.0%)에서 통계적으로 유의한 증가(감소)패턴을 보이는 것으로 분석되었다. 본 연구는 서로 다른 형태의 CT/WP El Ni$\tilde{n}$o가 한강 유역 수자원의 양과 발생시점의 변화에 민감하게 영향을 미치고 있음을 확인하였으며, 향후 다양한 형태의 ENSO에 따른 수문변량의 변화에 대한 진단연구를 통하여 장기적인 수자원관리 및 예측을 위한 기초 자료로 활용이 가능하리라 사료된다.

• 한국농림기상학회지
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• 제12권3호
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• pp.197-206
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• 2010

토지 이용이 다변화된 산지 유역에서 몬순 강우에 의한 환경 영향을 평가하는데 필수적인 기초 자료를 확보하기 위해 수질의 계절간 비교와 강우사상 집중샘플링의 방법을 활용하여 지표수 수질의 시공간적 변이 특성을 조사하였다. 유역 내 토지 이용을 반영하는 지표수 9개 지점을 대상으로 건 우기 수질의 계절적 차이를 비교하고, 2회의 강우사상에 대해 산림과 농경지 하천 2개 지점에서 집중 샘플링을 실시하였다. 대부분의 지점에서 건기보다 우기에 전기전도도와 $Cl^-$ 농도는 더 낮았으나, 총 금속 농도는 우기에 훨씬 더 높았다. 이는 우기에 늘어난 유량에 의해 용존 이온은 희석되고, 토양 침식량은 증가됐기 때문인 것으로 보인다. 한편 18mm의 적은 강우 시에 산림 하천의 수질에서는 거의 변화가 보이지 않은 데 반해, 농경지 하천에서는 부유토사와 용존 물질 농도가 모두 가파른 변화를 보였으며, 452mm의 많은 강우에 대해서는 농경지 하천은 물론 산림 하천에서도 큰 수질 변화를 관찰할 수 있었다. 농경지 하천의 Pb 농도는 부유토사 농도와 높은 양의 상관관계를 보였다. 제한된 샘플링 횟수와 조사지점으로 인해 결과 해석에 신중을 기해야 하겠지만, 전체 결과는 가파른 산지 유역에 농경지가 무분별하게 확장되면 강우의 변동폭과 극단화가 심해질 경우 토양 침식과 그에 따른 환경 영향의 취약도가 증가할 것임을 시사한다.

• Journal of Gastric Cancer
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• 제21권4호
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• pp.325-334
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• 2021

Purpose: Although dumping symptoms are thought to involve postprandial glycemic changes, postprandial glycemic variability without dumping symptoms remains poorly understood due to the lack of a method that allows the easy and continuous measurement of blood glucose levels. Materials and Methods: Patients having undergone distal gastrectomy with Billroth-I (DG-BI) or Roux-en-Y reconstruction (DG-RY), total gastrectomy with RY (TG-RY) and pylorus preserving gastrectomy (PPG) for gastric cancer 3 months to 3 years prior, diagnosed as pathological stage I or II, were prospectively enrolled from March 2018 to January 2020. The interstitial tissue glycemic levels were measured every 15 min, up to 14 days by continuous glucose monitoring. Moreover, using a diary recording the diet and symptoms, asymptomatic glucose profiles without sugar supplementation within 3 h postprandially were compared among the four procedures. Results: A total of 40 patients were enrolled, 10 patients for each of the four procedures. There were 47 glucose profiles with DG-BI, 46 profiles with DG-RY, 38 profiles with TG-RY, and 46 profiles with PPG. PPG showed the slowest increase with a subsequent gradual decrease in glucose fluctuations, without hyperglycemia or hypoglycemia, among the four procedures. In contrast, TG-RY and DG-RY showed spike-like glycemic variability, sharp rises during meals, and rapid drops. The glucose profiles of DG-BI were milder than those of RY. Conclusions: The asymptomatic glycemic changes after meals differ among the types of surgical procedures for gastric cancer. Given the mild glycemic fluctuations in PPG and the glucose spikes in TG-RY and DG-RY, pylorus preservation and physiological reconstruction without changes in food pathways may optimize postprandial glucose profiles after gastrectomy.

• Biomolecules & Therapeutics
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• 제23권5호
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• pp.486-492
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• 2015

Drug metabolism mostly occurs in the liver. Cytochrome P450 (CYP) is a drug-metabolizing enzyme that is responsible for many important drug metabolism reactions. Recently, the US FDA and EU EMA have suggested that CYP enzyme induction can be measured by both enzymatic activity and mRNA expression. However, these experiments are time-consuming and their interassay variability can lead to misinterpretations of the results. To resolve these problems and establish a more powerful method to measure CYP induction, we determined CYP induction by using luminescent assay. Luminescent CYP assays link CYP enzyme activity to firefly luciferase luminescence technology. In this study, we measured the induction of CYP isozymes (1A2, 2B6, 2C9, and 3A4) in cryopreserved human hepatocytes (HMC424, 478, and 493) using a luminometer. We then examined the potential induction abilities (unknown so far) of mesalazine, a drug for colitis, and mosapride citrate, which is used as an antispasmodic drug. The results showed that mesalazine promotes CYP2B6 and 3A4 activities, while mosapride citrate promotes CYP1A2, 2B6, and 3A4 activities. Luminescent CYP assays offer rapid and safe advantages over LC-MS/MS and qRT-PCR methods. Furthermore, luminescent CYP assays decrease the interference between the optical properties of the test compound and the CYP substrates. Therefore, luminescent CYP assays are less labor intensive, rapid, and can be used as robust tools for high-throughput CYP screening during early drug discovery.

• 대한의용생체공학회:의공학회지
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• 제33권2호
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• pp.59-64
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• 2012

With rapid increasing usage of smart phones, social concerns have arisen about the possible effects of electromagnetic fields emitted from wideband code division multiple access(WCDMA) mobile phones on human health. The number of people with self-reported electromagnetic hypersensitivity(EHS) who complain of various subjective symptoms such as headache, insomnia etc. has also recently increased. However, it is unclear whether EHS results from physiological or other origins. In this double-blinded study, we investigated physiological changes such as heart rate, respiration rate, and heart rate variability with real and sham exposures for 15 EHS and 17 non-EHS persons using a module inside a dummy phone. Experiment was conducted using a WCDMA module with average power of 24 dBm at 1950 MHz with the specific absorption rate of 1.57 W/kg using a headphone for 32 min. As a conclusion, WCDMA RF exposure did not have any effects on the physiological variables in either group.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1234-1239
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• 2006

Murrah and NiliRavi are the important North Indian buffalo breeds occupying the prominent position of being the highest milk producers. These breeds are more or less similar at morphological as well as physiological levels. The technique of RAPD-PCR was applied in the present study to identify a battery of suitable random primers to detect genetic polymorphism, elucidation of the genetic structure and rapid assessment of the differences in the genetic composition of these two breeds. A total of 50 random primers were screened in 24 animals each of Murrah and NiliRavi buffaloes to generate RAPD patterns. Of these, 26 (52%) primers amplified the buffalo genome generating 263 reproducible bands. The number of polymorphic bands for the 26 chosen RAPD primers varied from 3 (OPG 06 and B4) to 26 (OPJ 04) with an average of 10.1 bands per primer and size range of 0.2 to 3.2 kb. DNA was also pooled and analyzed to search for population specific markers. Two breed specific RAPD alleles were observed in each of Murrah (OPA02 and OPG16) and NiliRavi (OPG09) DNA pools. RAPD profiles revealed that 11 (4.2%) bands were common to all the 48 individuals of Murrah and NiliRavi buffaloes. Pair-wise band sharing calculated among the individual animals indicated considerable homogeneity of individuals within the breeds. Within breed, band sharing values were relatively greater than those of interbreed values. The low genetic distance (Nei's) value (0.109) estimated in this study is in accordance with the origin and geographical distribution of these breeds. The RAPD analysis indicated high level of genetic similarity between these two important North Indian buffalo breeds.

• 영재교육연구
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• 제11권1호
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• pp.99-117
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• 2001

본 연구에서는 만 6세 미만의 유아기 아동을 대상으로 영재판별의 필요성과 가능성을 살펴보고 지적 영재성을 진단할 수 있는 세부적인 방법들과 과제를 논의하였다. 6세미만의 유아기부터 지적 영재성은 더 나이든 아동의 특성과 유사한 행동특성으로 나타난다는 것이 부모의 관찰과 연구자들의 보고에 의해 밝혀져 있다. 단지 유아기에는 아동의 현재 수행보다 앞으로 보일 수행가능성 즉 잠재성의 측정이 중요하며 특히 유아의 여러 기능들이 서로 잘 분화되지 않은 점을 감안할 때 보다 과학적이고 포괄적인 판별이 필요하다는 것을 주장하였다. 구체적인 영재유아의 판별방법은 부모나 교사의 일상행동관찰에 의한 의뢰, 영재아 행동평가지, 간편 검사 그리고 개인별 세부 검사 등을 통한 종합적인 평가와 단계적 방법이 효율적임을 주장하였다. 특히 어린 유아의 경우 발달과정에서의 변이성이 크기 때문에 반복적 평가가 중요하다. 본 연구에서는 국내에서 사용하는 영재판별방법들의 종류와 검사자의 자질, 실시상의 주의점을 살펴보았으며 특히 영재아의 여러 특성을 올바로 진단할 수 있는 전문가와 다양한 검사방법이 개발이 중요하다는 것을 정리하였다. 끝으로 영재판별이 영재아에 대한 최적 교육환경의 제공이라는 점에서 조기 발견이 무엇보다 중요하다는 점을 강조하였다.

• International Journal of Safety
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• 제1권1호
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• pp.36-42
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• 2002

This paper is aimed at predicting the life of rubber insulating gloves under normal operating stresses from relatively rapid test performed at higher stresses. Specimens of rubber insulating gloves are subject to multiple stress conditions, i.e. combined electrical and thermal stresses. Two modes of electrical stress, step voltage stress and constant voltage stress are used in specimen aging. There are two types of test for electrical stress in this experiment: the one is Breakdown Voltage (BDV) test under step voltage stress and thermal stress and the other is lifetime test under constant voltage stress and temperature stress. The ac breakdown voltage defined as the break-down point of insulation that leakage current excesses a limit value, l0mA in this experiment, is determined. Because the very high variability of aging data requires the application of statistical model, Weibull distribution is used to represent the failure times as the straight line on Weibull probability paper. Weibull parameters are deter-mined by three statistical methods i.e. maximum likelihood method, graphical method and least squares method, which employ SAS package, Weibull probability paper and FORTRAN, respectively. Two chosen models for predicting the life under simultaneous electrical and thermal stresses are inverse power model and exponential model. And the constants of life equation for multistress aging are calculated using numerical method, such as Gauss Jordan method etc.. The completion of life equation enables to estimate the life at normal stress based on the data collected from accelerated aging test. Also the comparison of the calculated lifetimes between the inverse power model and the exponential model is carried out. And the lifetimes calculated by three statistical methods with lower voltage than test voltage are compared. The results obtained from the suggested experimental method are presented and discussed.

• Journal of Pharmaceutical Investigation
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• 제21권1호
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• pp.33-41
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• 1991

A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of theophylline(TP) and its metabolites, 1-methyluric acid (1-MU) and 1,3-dimethyluric acid (1,3-DMU), in rat plasma and urine. An $100\;{\mu}l$ aliquot of a plasma or urine sample was mixed with $250\;{\mu}l$ of acetonitrite and vortexed. After centrifugation, $200\;{\mu}l$ (plasma) or $20\;{\mu}l$ (urine) aliquot of the supernatant was dried by $N_2$ stream and redissolved in $100\;{\mu}l$ (plasma) or $200\;{\mu}l$ (urine) of the mobile phase. A $20\;{\mu}l$ of the mobile phase solution was injected onto a $C_{18}$ reversed-phase column. The column was maintained at $45^{\circ}C$ by the aid of electric heating jacket. The mobile phase was a 3%(v/v) methanol solution in deionized water which contains sodium acetate (100 mM) and tetrabutyl ammonium hydroxide (4 mM). pH of the mobile phase was adjusted 4.5 by the addition of acetic acid. Detection limits for TP, 1-MU, and 1,3-DMU in plasma were 0.2, 0.1 and $0.1\;{\mu}/ml$, respectively and the corresponding values in urine were all $5\;{\mu}g/ml$. Inter- and intra-day variability of the assay for all compounds in the plasma samples was less than 5.5 and 3.8%, respectively. The retention times for 1-MU, 1,3-DMU, and TP were approximately 7, 8.5 and 18 min, respectively. Sample preparation procedure used in this method was simple, rapid and reproducible. Renal clearance of TP and its metabolites in rats showed plasma concentration dependency indicating renal tubular secretion and reabsorption of them.

• Asian-Australasian Journal of Animal Sciences
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• 제16권1호
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• pp.116-123
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• 2003

Forages are the most important feed resource for ruminants worldwide, whether fed as pastures, forage crops or conserved hay, silage or haylage. There is large variability in the quality of forages so measurement and prediction of feeding value and nutritive value are essential for high levels of production. Within a commercial animal production system, methods of prediction must be inexpensive and rapid. At least 50% of the variation in feeding value of forages is due to variation in voluntary feed intake. Identification of the factors that constrain voluntary feed intake allows these differences to be managed and exploited in forage selection. Constraints to intake have been predicted using combinations of metabolic and physical factors within the animal while simple measurements such as the energy required to shear the plant material are related to constraints to intake with some plant material. Animals respond to both pre- and post-ingestive feedback signals from forages. Pre-ingestive signals may play a role in intake with signals including taste, odour and texture together with learned aversions to nutrients or toxins (post-ingestive feedback signals). The challenge to forage evaluation is identification of the factors which are most important contributors to these feedback signals. Empirical models incorporating chemical composition are also widely used. The models tend to be useful within the ranges of the datasets used in their development but none can claim to have universal application. Mechanistic models are becoming increasingly complex and sophisticated and incorporate both feed characteristics and use of biochemical pathways within the animal. Improvement in utilisation through the deliberate selection of pasture plants for high feeding value appears to have potential and has been poorly exploited. Use of Near Infrared Reflectance Spectroscopy is a simple method that offers significant potential for the preliminary screening of plants with genetic differences in feeding value. Near Infrared Reflectance Spectroscopy will only be as reliable as the calibration sets from which the equations are generated.