• 대한임상검사과학회지
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• 제39권3호
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• pp.161-166
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• 2007

To develop a rapid and reliable single-tube-based PCR technique for detection simultaneously the quinolone resistance qnrA, qnrB and qnrS genes. After multiple alignment, primers were designed to detect known qnr variants. I was used for A total of 43 extented-spectrum ${\beta}$-lactamases (ESBLs) producing Escherichia coli and Klebsiella pneumoniae isolated from university hospital were tested for screening, as with qnr genes. In optimized conditions, all positive controls confirmed the specificity of the PCR primers. Out of 43 isolates, qnrA genes were detected 19 (44.2%), qnrB genes 5 (11.7%), qnrS genes 15 (34.9%) and 8 (18.6%) isolates were not detected. I report here a fast and reliable technique for rapid screening of qnr positive strains to be used for epidemiological surveys.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2009년도 춘계 학술대회 제21권1호
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• pp.467-468
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• 2009

철근콘크리트 학교건물(Part 2의 대상건물)을 대상으로 일본내진진단법(2차 및 3차) 및 비선형 정적 및 동적해석을 실시하여 상세하게 대상건물의 내진성능을 평가한 결과와 본 연구에서 제안한 내진성능평가표를 이용하여 평가한 내진성능(Part-2)을 각각 비교 검토하여 본 연구의 간이 내진성능 평가법의 타당성을 검증하였다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2009년도 춘계 학술대회 제21권1호
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• pp.463-464
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• 2009

본 연구에서는 전단 및 휨파괴형 부재가 혼합된 저층 철근콘크리트 거물의 내진성능을 간편하면서도 효율적으로 평가가 가능한 간이 내진성능 평가법을 제안함과 동시에 단시간 내에 약산적으로 내진성능이 우수한 건물을 우선적으로 선별이 가능하도록 피해도 판정식과 내진성능 평가식을 제안하여 정략적으로 평가가 가능한 내진성능 평가표를 개발하였다. 상기 평가법을 이용하면, 내진성능 평가점수와 그에 대응하는 지진손상도가 추정가능하여 간편 효율적으로 내진성능을 평가할 수 있다.

• 한국공간구조학회논문집
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• 제13권3호
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• pp.57-64
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• 2013

Since the terrorist attacks 9.11 in 2001, the public places such as subway, department stores or office buildings have become a target of terror in major public facilities. In this paper, for the prevention of terrorist attack in domestic building, the assessments of terror risk were conducted and their relationship with building heights was discussed through FEMA 455-Rapid Visual Screening(RVS). Explosive terror risk tends to increase rapidly with building heights(H<230m), showing the correlation coefficient between total risk and building heights(H<230m) is 0.93. In addition, The average of consequences is 8.47 and that of' threat is 8.95. Vulnerability is found to be 6.62 in average.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.118.3-119
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• 2003

Diazepam (DZ) is one of the most frequently prescribed drugs as an antianxiety agent, muscle relaxant, and anticovulsant and sometimes causes intoxication due to accidental overdose, misuse or abuse. Screening or confirmation methods for DZ and NDZ in plasma are very important for clinical and toxicological studies and in forensic cases. GC/MS assay with SPE was developed for the determination of diazepam and its metabolite, nordiazepam in human plasma. Diazepam in plasma was extracted by a rapid and sensitive procedure based on C18 bonded-phase extraction. (omitted)

• Earthquakes and Structures
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• 제16권1호
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• pp.29-39
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• 2019

A three-step screening process is presented in this article for selection of consistent earthquake records in which number of suitable ground motions is quickly screened and reduced to a handful number. Records that remain at the end of this screening process considerably reduce the dispersion of structural responses. Then, an effective method is presented for spectral matching and modification of the selected records. Dispersion of structural responses is explored using different statistical measures for each scaling procedure. It is shown that the Uniform Design Method, presented in this study for scaling of earthquake records, results in most cases in the least dispersion measure.

• 방사선산업학회지
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• 제18권2호
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• pp.101-107
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• 2024

Rapid screening for internal contamination by alpha- and beta-emitting radionuclides is essential in situations involving radiation workers or radiation accidents. This study focused on the use of urine samples and liquid scintillation counting to quickly and accurately assess contamination. Calibration of the alpha and beta detection areas ensured precise measurement results. The major radionuclides recommended for surveillance during accidents were also considered. This study evaluated the effectiveness of the method by examining various parameters, including the limit of detection, linearity, sensitivity, selectivity, accuracy, ruggedness, and blind test sample analysis. The liquid scintillation counting method is an effective tool for screening urinary samples to detect alpha- and beta-emitting radionuclides, particularly during radiation emergencies, despite some limitations in precision.

• Current Research on Agriculture and Life Sciences
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• 제31권1호
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• pp.1-10
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• 2013

The exposure of food to ionizing radiation has been recognized as a safe and effective mode of food preservation in more than 55 countries. The benefits include eradication of insect pests, inactivation of food pathogens, extension of shelf-life, and improvement in food hygiene. Regulatory authorities around the world have emphasized the implementation of various national and international regulations to facilitate trade and development of consumers' confidence in purchasing irradiated foods. Therefore, the need for reliable irradiation detection methods has increased to enforce these regulations. At present, a number of promising analytical approaches have been developed and evaluated. Moreover, about 10 European Standards have been adopted as General CODEX Alimentarius methods for the detection of irradiated foodstuffs. However, most of these methods demand relatively expensive equipment and prolonged sample preparation. Therefore, simple and cost-effective approaches would be advantageous for rapid screening of foodstuffs. The suspected samples need to be analyzed further with more validated techniques to confirm the screening results. In this review, existing screening methods (i.e. physical, chemical, and biological) for the identification of irradiated foods have been outlined along with their principles, scopes and limitations.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.232-234
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• 2003

• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1117-1123
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• 2019

Control of pine wilt disease, which is caused by pine wilt nematode Bursaphelenchus xylophilus, is heavily dependent on the use of chemicals such as abamectin. Although such chemicals are highly effective, demands for alternatives that are derived preferentially from natural sources, are increasing out of environmental concerns. One of the challenges to discovery of alternative control agents is lack of fast and efficient screening method that can be used in a high-throughput manner. Here we described the development of colorimetric assay for the rapid and accurate screening of candidate nematicidal compounds/biologics targeting B. xylophilus. Contrary to the conventional method, which relies on laborious visual inspection and counting of nematode population under microscope, our method utilizes a redox dye that changes its color in response to metabolic activity of nematode population in a given sample. In this work, we optimized parameters of our colorimetric assay including number of nematodes and amount of redox dye, and tested applicability of our assay for screening of chemicals and biologics. We demonstrated that our colorimetric assay can be applied to rapid and accurate quantification of nematode viability/mortality in a nematode population treated with candidate chemicals/biologics. Application of our method would facilitate high-throughput endeavors aiming at finding environment-friendly control agents for deadly disease of pine trees.