• Korean Journal of Animal Reproduction
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• v.16 no.1
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• pp.47-53
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• 1992

This study was carried out to investage the effect of cryoprotectant concentration and equilibration time on volume change and in vitro development of intact and bisected mouse embryos by rapid freezing. When compacted morulae were rapidly frozen in 3.0 to 4.0 glycerol or DMSO with 0.25M sucrose solution, the superior(P<0.05) post-thaw survival rate was obtained at the glycerol concentration of 4.0M(89.4%) than 3.0M(71.4%) or 5.0M(42.4%), but at the DMSO concentration of 3.0M(84.5%) than 4.0M(51.1%) or 5.0M(0.0%). The optimal equilibraton time for rapid freezing of ZP-free or bisected morulae in 4.0M glycerol with 0.25M sucrose was found tobe 3 minutes. The minimal volume of compacted morulaewhich corresponded with 61 to 62% of pre-equilibrated embryo volume was obtained from equilibration for 3 minutes in both 3.0 and 4.0M glycerol solutions with 0.25M sucrose.

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.171-178
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• 1993

The present study was carried out to investigate the developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos. The nuclei from 2-, 4- and 8-cell mouse embryos were transferred into enucleated 2-cell embryos, and the reconstituted embryos were submitted to direct current(DC) pulse at output voltage of 2.0 kV/cm for $100{\mu}$ sec to induce cell fusion. The recovery rate and developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos was investigated. 1. The recovery rate of nuclear transplanted 2-cell embryos in normal morphology after freezing and thawing was significantly higher in rapid freezing(DMSO 4.5M) than in slow cooling(p<0.01). 2. When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant. In summary, these experiments have proved that rapid freezing method(DMSO 4.5M) is effective in nuclear transplanted 2-cell mouse embryos. If improved micromanipulation techniques and freezing are combined, nuclear transplantation technique will contribute to the improvement of productivity in livestock animals.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.4
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• pp.46-55
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• 2021

Concrete structures can cause various problems as the number of common years increases when exposed to external extreme climate conditions. Among these problems, freezing and thawing occur due to the action of extreme climate factors such as heavy rain and heavy snow, which have become the most problematic in recent years. In this study, we present a rapid freezing and thawing test method of concrete in the air, referring to KS F 2456, as Seoul exhibits very dry weather during the period of freezing and thawing. Concrete test specimens and RC beams were fabricated to perform rapid freezing and thawing of 0, 100, 200, and 300 cycles, and the performance evaluation confirmed the degradation of each subject in material and member units. The design strength of 24 MPa, which performs rapid freezing and thawing in the air up to 300 cycles, decreases by 5.24 MPa (21%), and as rapid freezing and thawing in the air increases the stress burden on reinforced concrete bending members, reducing the energy absorption (dissipation) ability of structures due to earthquakes.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 1999.10c
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• pp.294-300
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• 1999

After researching the physical properties of the concrete included Rice Husk Ash(RHA concrete) and workability of fresh concrete admixed RHA, we have tested durability of RHA-concrete against freeaing and thawing in the winter using rapid freezing and thawing test method(KS F 2456) . There aretwo hypotheses to explain the failure mechanism of a freezing and thawing action. First, the hydraulic pressure in the pores of freezing concrete make an internal stress of concrete structures outbreaking micro crack in the face of concrete, Second, Frost action causing damage to cement paste repeatedly come from soil frost action, freezing water in the capillaries. Initial Relative Dynamic Modulus of Elasticity (DME) was biggest in cae of unit binder weight 600kgf/㎥ and relative dynamic modulus of elasticity increased until 300cycles. In general , initial relative DME was proportional to unit binder weight . Relative DME was decreased in proportion to unit binder weight in the case of 300, 400, 500kgf/㎥ , but relative DME fo the others remained more than 90% until 300 cycles. It was not good effect of intermixed RHA to concrete in case of below unit binder weight 300kgf/㎥ and the resistance of freezing and thawing was not good either.

• Journal of Embryo Transfer
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• v.4 no.1
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• pp.1-13
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• 1989

This paper reviews the most important steps that have generated consistent progress in principles and developmental progress of embryo cryopreservation, and also study on freezing procedure and its application by conventional method and current improved method for freezing procedure and its appilcation of embryo cryopreservation in farm animals. Four were of particular interest: 1.The transport of water across the ccli membrane (zona pellucida) during freezing and thawing accordinglyplays a role in determing whether the celi survives. This movement of water is controlied mainly by extracellular phase changes and by the nature and concentration of any cryoprotective agent present. Therates of cooling, freezing and warming, and the intervals over which they are applied are further decisi've factors in determining whether a cryopreservation procedure allows survival after thawing. 2.The first successful deep freezing experiments with sheep morula and blastocysts during the seventies were based on the early procedures used for mouse embryos.Current research during the eighties is developed with the aim of simplifying and improving current procedures such as one-step dilution and rapid or ultra-rapid cooling by using the model of laboratory animals. 3.The conventional method for the embryo cryopreservation is described. An alternative to this method which may result in high survival and also in reducing of the freezing and thawing time is done by combing a permeable cryoprotectant such as glycerol, DMSO or propanediol and a non-permeable compound such as sucrose, trehalose, raffinose or lactose. 4.Finally a different approach to the preservation of embryos, named vitrification, is introduced. This procedure depends upon the ability of concentrated solutions of cryoprotective agents such as glycerol and propanediol to supercool to very low temperature (-196$^{\circ}C$) during rapid cooling before solidifying without formation of ice. However, more complete data are necessary for successful vitrification of blastocysts.

• Journal of the Korea institute for structural maintenance and inspection
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• v.5 no.1
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• pp.131-140
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• 2001

In general, the deterioration induced by the freezing and thawing cyclic in concrete structures often leads to the reduction in concrete durability by the cracking or surface spalling. If it can prediction of concrete deterioration subjected to cyclic freezing and thawing, we can rationally do the design of mix proportion in view of concrete durability and the maintenance management of concrete structures. Therefore in this study a prediction method of deterioration for concrete structures subjected to the irregular freezing and thawing is proposed from the results of accelerated laboratory freezing and thawing test using the constant temperature condition and the in-situ weathering data. Furthermore, to accurately predict the concrete deterioration, a method of modification for the effect of hydration increasing during rapid freezing and thawing test is investigated.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.2
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• pp.129-134
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• 1998

The objective of this study was to test whether the developmental capacity of human multi-pronuclear (PN) zygotes after ultra-rapid freezing using EM grid can be maintained. For this experiment, multi-PN zygotes which produced in human IVF program were used as an alternatives of normal 2PN zygotes, and they were separated into 3PN or $\geq4PN$ zygotes to compare their in vitro development and cryoinjury according to PN number. As freezing solution, EFS30 which consisted of 30% ethylene glycol, 18% bcoll, 0.5 M sucrose and 10% FBS added D-PBS was used. The result obtained in this experiment was summarized as follows; When the multi..PN zygotes were ultrarapidly frozen and thawed, the high mean percentages (85.5%) were survived. Also when the cleavage rates between control and freezing group were compared with PN number, there were not significantly different in each group (3PN; 81.3% & 85.4% and $\geq4PN$; 90.0% & 95.7%). When the in vitro development rates after thawing were examined, freezing 3PN group (22.0%) was not differed to control 3PN group (38.5%), although the development result of freezing $\geq4PN$ group (45%) was significantly lower than that of control $\geq4PN$ group (44.4%) (p<0.05). These results demonstrate that developmental capacity of human zygote can be obtained by ultra-rapid freezing method using EM grid and EFS30.

• Applied Microscopy
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• v.20 no.2
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• pp.1-11
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• 1990

The ultrastructure of freeze-substituted Malpighian tubule cells of German cockroach after rapid freezing was compared to that fixed with conventional chemical fixation. As results, freeze-substitution proved to be the superior technique revealing much more structural details of the tissue cells. Especially, all kinds of membrane structures including infoldings, microfilament, microtubules and ribosomes were best preserved in this material.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.05a
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• pp.625-630
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• 2002

The availability of natural aggregates has decreased in recent years due to depleting reserves. From the viewpoint of energy and resources saving, it may be advantageous to use the waste concrete as construction aggregates. The purpose of this study is to analyze petrographic and chemical properties of recycled aggregate, and to investigate the resistance of concrete to rapid freezing and thawing by using replacement ratios (0, 10, 20, 30, 40, 50, 60, 70, 100% ) of recycled aggregate.

• Food Science of Animal Resources
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• v.36 no.5
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• pp.650-655
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• 2016

This study investigated the effects of artificial supercooling followed by still air freezing (SSF) on the qualities of pork loin. The qualities of pork frozen by SSF were compared with the fresh control (CT, stored at 4℃ for 24 h), slow freezing (SAF, still air freezing) and rapid freezing (EIF, ethanol immersion freezing) treatments. Compared with no supercooling phenomena of SAF and EIF, the extent of supercooling obtained by SSF treatment was 1.4℃. Despite that SSF was conducted with the same method with SAF, application of artificial supercooling accelerated the phase transition (traverse from -0.6℃ to -5℃) from 3.07 h (SAF) to 2.23 h (SSF). The observation of a microstructure indicated that the SSF prevented tissue damage caused by ice crystallization and maintained the structural integrity. The estimated quality parameters reflected that SSF exhibited superior meat quality compared with slow freezing (SAF). SSF showed better water-holding capacity (lower thawing loss, cooking loss and expressible moisture) and tenderness than SAF, and these quality parameters of SSF were not significantly different with ultra-fast freezing treatment (EIF). Consequently, the results demonstrated that the generation of supercooling followed by conventional freezing potentially had the advantage of minimizing the quality deterioration caused by the slow freezing of meat.