• 제목/요약/키워드: rapid extraction method

검색결과 329건 처리시간 0.034초

RapidEye 위성영상의 시계열 NDVI 및 객체기반 분류를 이용한 북한 재령군의 논벼 재배지역 추출 기법 연구 (Extraction of paddy field in Jaeryeong, North Korea by object-oriented classification with RapidEye NDVI imagery)

  • 이상현;오윤경;박나영;이성학;최진용
    • 한국농공학회논문집
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    • 제56권3호
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    • pp.55-64
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    • 2014
  • While utilizing high resolution satellite image for land use classification has been popularized, object-oriented classification has been adapted as an affordable classification method rather than conventional statistical classification. The aim of this study is to extract the paddy field area using object-oriented classification with time series NDVI from high-resolution satellite images, and the RapidEye satellite images of Jaeryung-gun in North Korea were used. For the implementation of object-oriented classification, creating objects by setting of scale and color factors was conducted, then 3 different land use categories including paddy field, forest and water bodies were extracted from the objects applying the variation of time-series NDVI. The unclassified objects which were not involved into the previous extraction classified into 6 categories using unsupervised classification by clustering analysis. Finally, the unsuitable paddy field area were assorted from the topographic factors such as elevation and slope. As the results, about 33.6 % of the total area (32313.1 ha) were classified to the paddy field (10847.9 ha) and 851.0 ha was classified to the unsuitable paddy field based on the topographic factors. The user accuracy of paddy field classification was calculated to 83.3 %, and among those, about 60.0 % of total paddy fields were classified from the time-series NDVI before the unsupervised classification. Other land covers were classified as to upland(5255.2 ha), forest (10961.0 ha), residential area and bare land (3309.6 ha), and lake and river (1784.4 ha) from this object-oriented classification.

A Rapid and Simple Method for DNA Preparation of Magnaporthe oryzae from Single Rice Blast Lesions for PCR-Based Molecular Analysis

  • Liying, Dong;Shufang, Liu;Jing, Li;Didier, Tharreau;Pei, Liu;Dayun, Tao;Qinzhong, Yang
    • The Plant Pathology Journal
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    • 제38권6호
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    • pp.679-684
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    • 2022
  • Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M. oryzae, the direct extraction of M. oryzae genomic DNA from infected rice tissue would be useful alternative for rapid monitoring of changes of avirulence genes without isolation and cultivation of the pathogen. In this study, a fast, low-cost and reliable method for DNA preparation of M. oryzae from a small piece of infected single rice leaf or neck lesion was established. This single step method only required 10 min for DNA preparation and conventional chemical reagents commonly found in the laboratory. The AvrPik and AvrPi9 genes were successfully amplified with the prepared DNA. The expected DNA fragments from 570 bp to 1,139 bp could be amplified even three months after DNA preparation. This method was also suitable for DNA preparation from M. oryzae strains stored on the filter paper. All together these results indicate that the DNA preparation method established in this study is reliable, and could meet the basic needs for polymerase chain reaction-based analysis of M. oryzae.

교반막대 추출법과 GC/MS-MS를 이용한 수중의 자외선 차단제 분석 (Analysis of UV Filters in Water using Stir Bar Sorptive Extraction (SBSE) and GC/MS-MS)

  • 서창동;손희종;정종문;최진택;장성호
    • 한국환경과학회지
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    • 제23권6호
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    • pp.1037-1047
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    • 2014
  • A highly sensitive analytical method based on stir bar sorptive extraction (SBSE) technique and gas chromatography/tandem mass spectrometry (GC/MS-MS) has been developed, allowing the simultaneous multi-analyte determination of seven UV filters in water samples. The stir bar coated with polydimethylsiloxane (PDMS) was added to 40 mL of water sample at pH 3 and stirred at 1,100 rpm for 120 min. Other SBSE parameters (salt effect and presence of organic solvent) were optimised. The method shows good linearity (coefficients > 0.990) and reproducibility (RSD < 12.9%). The extraction efficiencies were above 84% for all the compounds. The limits of detections (LOD) and limits of quantification (LOQ) were 2.1~8.6 ng/L and 6.8~27.5 ng/L, respectively. The developed method offers the ability to detect 8 UV filters at ultra-low concentration levels with only 40 mL of sample volume. Matrix effects in tap water, river water, wastewater treatment plant (WWTP) final effluent water and seawater were investigated and it was shown that the method is suitable for the analysis of trace level of 7 UV filters except of benzophenone (BP). The method developed in the present study has the advantage of being rapid, simple, high-sensitive and both user and environmentally friendly.

객체기반 도로모델을 이용한 고해상도 위성영상에서의 도로 추출 (Road Extraction from High Resolution Satellite Image Using Object-based Road Model)

  • 변영기;한유경;채태병
    • 대한원격탐사학회지
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    • 제27권4호
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    • pp.421-433
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    • 2011
  • 도시 정보시스템 및 위치기반 서비스와 같은 공간정보 분야의 빠른 성장으로 인해 도심지 도로정보 취득 및 갱신에 대한 중요성이 날로 증가하고 있다. 본 연구에서는 고해상도 위성영상으로부터 도로 정보를 추출하기 위하여 최근 화소기반분석의 대안으로 주목을 받고 있는 객체기반 접근법을 이용한 자동 도로추출 방법을 제안한다. 이를 위해 우선 MSRG(Modified Seeded Region Growing)기법을 이용하여 공간객체를 생성한 후, 객체의 형상 특정정보와 인접성을 기반으로 핵심 도로 객체를 자동으로 추출하였다. 또한 추출된 핵심도로 객체와 인접한 객체들과의 공간적 상관성을 이용하여 일부 누락된 도로객체를 추적하였다. 최종적으로 도로의 기하학적인 특성을 이용한 단절된 도로 구간 연결 및 도로 변형 개선 과정을 통하여 최종도로영역을 추출하였다. 제안 기법의 성능 검증을 위한 정량적 평가 결과, 도로영역에 대해 높은 탐지정확도를 보임을 확인하였다. 결과적으로 제안된 방법은 고해상도 위성영상의 도로추출에 유용하게 적용될 수 있으리라 판단된다.

Optimization of One-step Extraction/Methylation Method for Analysis of Fatty Acid Composition in Brown Rice

  • Cho, Kyoung-Shim;Kim, Hyun-Ju;Moon, Sang-Mi;Kang, Jung-Hoon;Lee, Young-Sang
    • 한국작물학회지
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    • 제51권1호
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    • pp.89-94
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    • 2006
  • Traditionally fatty acid composition used to be analysed by a GC and the sample preparation process includes lipid extraction from sample and subsequent methyl esters preparation, which are time-consuming and cumbersome. As an alternative, simultaneous extraction/methylation methods are being developed for rapid and simplified sample preparation. To optimize one-step extraction/methylation method for analysis of fatty acid composition in brown rice, various reaction factors such as sample to reaction solution ratio, reaction time and temperature, shaking intensity were changed and resultant fatty acid composition data were evaluated in comparison with previous reports. The ratio of sample weight to reaction solution volume was the most critical factor in that higher sample to reaction solution ratio caused overestimation of palmitic acid and linoleic acid composition, resulting in underestimation of oleic acid. Lower reaction temperature also induced overestimation of linoleic acid and underestimation of oleic acid. Reaction duration and the intensity of shaking prior to and during the reaction, however, caused no significant changes in analysis results. In conclusion, the optimum condition was mixing 5 grains (about 0.2 g) of brown rice with $680{\mu}L$ of extraction/methylation mixture and $400{\mu}L$ of heptane, followed by reaction at $80^{\circ}C$ for 2 hours.

수입 축산식품에서의 잔류농약 동시분석법 개발에 관한 연구 (Study on the development of simultaneous-rapid screening methods for residues pesticides in livestock products)

  • 김종수;김곤섭;하대식
    • 대한수의학회지
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    • 제37권1호
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    • pp.213-220
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    • 1997
  • A simple and rapid method known as matrix solid phase dispersion(MSPD) for simultaneous determination of 11 pesticide(2,4,5,6-tetrachlor m-xylene, ${\alpha}$-BHC, ${\gamma}$-BHC, ${\delta}$-BHC, aldrin, chlorfulazuron, heptachloroepoxide, dieldrine, endrin, endosulfan sulfate, and tetradifon) in beef fat was estabilished. Beef fat(0.5g) was fortified by adding the 11 pesticides and dibutylchlorendate as internal standard, and blended with 2g bulk $C_{18}$ in pestle and motar. Pesticides were eluted from an extraction column composed of $C_{18}$/ fat matrix blend and 2g activated Florisil by addition of 8ml acetonitrile. Then $2{\mu}l$ portion of the acetonitrile elute was directly analyzed by gaschromatography with electron capture detection. Unfortified blank control were treated similarly. Recovery rate were ranged from $83{\pm}5.4%$ to $94.2{\pm}7.6%$, intra-assay variability and inter-assay variability were ranged from 2.3% to 7.4%(n=5 for each insecticides) and from $6{\pm}1%$ to $12{\pm}3%$(n=10 for each insecticides), respectively. These results indicated that the MSPD methodology is aceptable for the extraction, determination and screening of residues 11 chroniated pesticides in beef fat.

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즉시 탈회 치아이식재를 사용한 치조골 재건술 (Immediate Autogenous Fresh Demineralized Tooth (Auto-FDT) Graft for Alveolar Bone Reconstruction)

  • 이은영
    • 대한치과의사협회지
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    • 제54권5호
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    • pp.348-355
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    • 2016
  • Ideal autogenous or allogenic bone graft materials should provide 1) stabilization of blood clot, 2) scaffolds for cellular proliferation and differentiation, 3) release of osteogenic growth factors, 4) appropriate resorption profile for remodeling of new bone. Teeth, especially dentin, mostly contain hydroxyapatite and type I collagen which are similar to bone, and could be valuable graft material. Clinically teeth are used as calcined or demineralized forms. Demineralized form of dentin can be more effective as a graft material. But a conventional decalcification method takes time and long treatment time may give negative effects to various osteogenic proteins in dentin. Author used a new clinical method to prepare autogenous teeth, which could be grafted into the removal defects immediately after extraction using vacuum ultrasonic system. The process could be finished within two hours regardless of the form (powder, chip or block). Teeth were processed to graft materials in block, chip, or powder types immediately after extraction. It took 120 minutes to prepare block types and 40 minutes to prepare powder. Clinical cases did not show any adverse response and the healing was favorable. Rapid preparation of autogenous teeth with the vacuum ultrasonic system could make the immediate one-day extraction and graft possible.

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A Simple and Simultaneous Analysis of Volatile Halogenated Hydrocarbons in Indoor Air Using Personal Sampler

  • Jung, Won-Tae;Sohn, Dong-Hun
    • Journal of Korean Society for Atmospheric Environment
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    • 제9권E호
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    • pp.373-381
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    • 1993
  • To understand the human exposure levels of volatile halogenated hydrocar-bons in ambient air, a new rapid and convenient analytical method for determination of the compounds in gaseous phase was evaluated and established. The method is based upon passsive diffusion to personal sampler containing adsorbent and solvent extraction followed by purge trap/ on-column cryof-ocusing method. A new method needs no special instrumentation for gas collection because it is based upon the passive diffusion principle. The typical chromatogram obtained in this study proved that rapid and simultaneous determination of target analytes was possible with good resolution. The developed method was successfully applied to determine the volatile halogenated hydrocarbons in indoor air and the values obtained by this new method were compared with those by direct suction method. The concentration of carbon tetrachloride, 1,1,2-trichloroethylene, chloroform showed the values below 400$\mug/m^3$ except the maximum of 1,513$\mug/m^3$ of chloroform. 1,1,1-Trichloroethane showed approximately 1,000 to 5,000$\mug/m^3$ range of diurnal fluctuation in indoor air.

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A New Analytical Method for Erythromycin in Fish by Liquid Chromatography/Tandem Mass Spectrometry

  • Park, Mi-Jung;Park, Mi-Seon;Lee, Tae-Seek;Shin, Il-Shik
    • Food Science and Biotechnology
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    • 제17권3호
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    • pp.508-513
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    • 2008
  • Erythromycin has been used to treat Streptococosis, Edwardsiel1osis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electro spray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, $87.6{\pm}5.0%$; black rockfish, $87.2{\pm}6.4%$; eel, $85.2{\pm}4.8%$; and rainbow trout, $86.0{\pm}6.2%$. In the established method in this study, the correlation of coefficient values ($R^2$) of erythromycin calibration curve (n=11) was 0.9998.

Rapid Identification of Staphylococcus Species Isolated from Food Samples by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

  • Kim, Eiseul;Kim, Hyun-Joong;Yang, Seung-Min;Kim, Chang-Gyeom;Choo, Dong-Won;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • 제29권4호
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    • pp.548-557
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    • 2019
  • Staphylococcus species have a ubiquitous habitat in a wide range of foods, thus the ability to identify staphylococci at the species level is critical in the food industry. In this study, we performed rapid identification of Staphylococcus species using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS was evaluated for the identification of Staphylococcus reference strains (n = 19) and isolates (n = 96) from various foods with consideration for the impact of sample preparation methods and incubation period. Additionally, the spectra of isolated Staphylococcus strains were analyzed using principal component analysis (PCA) and a main spectra profile (MSP)-based dendrogram. MALDI-TOF MS accurately identified Staphylococcus reference strains and isolated strains: the highest performance was by the EX method (83.3~89.5% accuracy) at species level identification (EDT, 70.3~78.9% accuracy; DT, less than 46.3~63.2% accuracy) of 24-h cultured colonies. Identification results at the genus level were 100% accurate at EDT, EX sample preparation and 24-h incubation time. On the other hand, the DT method showed relatively low identification accuracy in all extraction methods and incubation times. The analyzed spectra and MSP-based dendrogram showed that the isolated Staphylococcus strains were characterized at the species level. The performance analysis of MALDI-TOF MS shows the method has the potential ability to discriminate between Staphylococcus species from foods in Korea. This study provides valuable information that MALDI-TOF MS can be applied to monitor microbial populations and pathogenic bacteria in the food industry thereby contributing to food safety.