• Title/Summary/Keyword: rapid extraction method

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고체상 추출과 GC/MS를 이용한 소변 중 대마 대사체 (THCCOOH) 분석 (Determination of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCCOOH) in human urine by solid-phase extraction and GC/MS)

  • 정재철;김진영;인문교;정원조
    • 분석과학
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    • 제19권5호
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    • pp.441-448
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    • 2006
  • 소변 중 대마 남용여부를 판별하는데 기준이 되는 tetrahydrocannabinol (THC)의 대사체 성분인 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THCCOOH)를 고체상 추출법 (solid-phase extraction, SPE)과 가스크로마토그래피/질량분석법 (GC/MS)을 이용하여 신속하게 분석 할 수 있는 방법을 제시하였다. 본 실험은 시험관에 소변 3 mL를 취해 염기성 (pH 10) 조건에서 가수분해 한 후, 양이온교환 카트리지를 사용하여 THCCOOH 성분을 선택적으로 추출하고, 증발 건고한 다음 유도체 반응을 시켜 GC/MS로 분석하였다. 그 결과 분석방법의 검출한계 (LOD)는 0.4 ng/mL이고, 정량한계 (LOQ)는 1.2 ng/mL이였다. 검정곡선의 직선성 상관계수 ($r^2$)는 1.2 (LLE는 1.3)~50.0 ng/mL의 농도범위에서 0.999를 나타내었다. 그리고 정밀도 (precision)와 정확도 (accuracy)는 모두 ${\pm}1.20%$ 이내로 안정적이었으며, 회수율(recovery)은 83.6~90.7%로 측정되었다. 액체상 추출법 (liquid-liquid extraction, LLE)과 비교할 때, SPE 방법이 회수율은 낮았지만 검출한계, 정량한계, 정밀도 및 정확도에서는 큰 차이가 없었다. 그러나 LLE 방법은 추출과정에 시간과 노력이 많이 드는 반면, SPE 방법은 상대적으로 추출 조작이 간편하고 신속하게 추출되었으며, 추출 잔류물도 깨끗하였다. SPE를 이용한 추출방법을 다수의 대마 흡연자 소변에 적용하였을 때 기존에 사용하던 LLE 방법보다 간편하고, 신속하게 대마 대사체 분석이 가능하였다.

Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry

  • Ahn, Jang-Hyuk;Kwak, Byung-Man;Park, Jung-Min;Kim, Na-Kyeoung;Kim, Jin-Man
    • 한국축산식품학회지
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    • 제34권6호
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    • pp.749-756
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    • 2014
  • A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HCl) to chemical extraction. Then, chloroform was used for removing a lipid fraction. After centrifuged, L-carnitine was separated and quantified using LC-MS/MS with electrospray ionization (ESI) mode. The precursor ion for L-carnitine was m/z 162, and product ions were m/z 103 (quantitative) and m/z 85 (qualitative), respectively. The results for spiked recovery test were in the range of 93.18-95.64% and the result for certified reference material (SRM 1849a) was within the range of the certificated values. This method could be implemented in many laboratories that require time and labor saving.

RF-LPP법을 이용한 고순도 마이크로 Ag 입자 합성 (The synthesis of high purity micro Ag particle using the rapid firing -liquid phase precursor method)

  • 임병석;송영현;이민지;맹성렬;윤대호
    • 한국결정성장학회지
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    • 제25권3호
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    • pp.93-97
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    • 2015
  • 본 연구에서는 고순도 균질 마이크로 Ag 입자를 얻기 위하여 용매추출법과 RF-LPP법을 이용하여 Air 분위기에서 $500^{\circ}C$에서 3시간 동안 열처리하여 Ag 입자를 합성하였다. 합성된 마이크로 Ag 입자를 XRD에 의해 비교 분석한 결과 주요 peak들이 JCPDS card(No. 87-0719)와 일치하는 것을 확인하였다. RF-LPP법을 이용함으로써, 핵 생성 싸이트 제어를 통해 균질 마이크로 Ag 입자를 합성하였으며, 환원처리 후 산소의 함유가 줄어드는 것을 확인하였다. 본 연구를 통하여 재활용 기술에 큰 기여를 할 것이라 기대한다.

Solvent Extraction, Preconcentration and Determination of Thorium with Monoaza 18-Crown-6 Derivative

  • Dolak, I.;Karakaplan, M.;Ziyadanogullar, B.;Ziyadanogullari, R.
    • Bulletin of the Korean Chemical Society
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    • 제32권5호
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    • pp.1564-1568
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    • 2011
  • A solvent extraction separation, preconcentration and determination of thorium with a new crown, 2-ethyl-N-benzyl-4,7,10,13,16-pentaoxa-1-azacyclooctadecane (MACE), is described in the study. The amount of thorium in the aqueous phase and organic phase was determined by Inductively Coupled Plasma-Optical Emission Spectroscopy and Ultraviolet-Visible, respectively. Thorium loaded organic phase was quantitatively stripped in a stage by using 1.0 M $HNO_3$. Thorium was effectively extracted with MACE in the pH range of 6-7 to produce a 3:2 complex ratio in the chloroform. A highly sensitive and rapid spectrophotometric method was described for determination of trace amounts of thorium with MACE. The effective molar absorption coefficient at 281 nm is $1.98{\times}10^3\;mol^{-1}cm^{-1}$, and the system complies with Beer's law in the range from 0.464 to 2.32 ${\mu}gm\;L^{-1}$ of thorium. Thorium was also determined in standard and environmental samples.

Urban Road Extraction from Aerial Photo by Linking Method

  • Yang, Sung-Chul;Han, Dong-Yeo;Kim, Min-Suk;Kim, Yong-Il
    • Korean Journal of Geomatics
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    • 제3권1호
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    • pp.67-72
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    • 2003
  • We have seen rapid changes in road systems and networks in urban areas due to fast urbanization and increased traffic demands. As a result, many researchers have put greater importance on extraction, correction and updating of information about road systems. Also, by using the various data on road systems and its condition, we can manage our road more efficiently and economically. Furthermore, such information can be used as input for digital map and GIS analysis. In this research, we used a high resolution aerial photo of the roads in Seongnam area. First, we applied the top-hat filter to the area of interest so that the road markings could be extracted in an efficient manner. The lane separation lines were selected, considering the shape similarity between the selected lane separation line and reference data. Next, we extracted the roads in the urban area using the aforementioned road marking. Using this technique, we could easily extract roads in urban area in semi-automatic way.

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황금(Scutellaria Radix)의 에타놀 추출물이 백서 치조골 형성에 미치는 영향 (Effects of Ethanolic Extracts of Scutellaria Radix on the alveolar bone formation in the extract socket of rat)

  • 박준봉;허익;권영혁;배기환;정종평
    • Journal of Periodontal and Implant Science
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    • 제27권3호
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    • pp.443-457
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    • 1997
  • The purpose of this study was to evaluate the effects of ethanolic extracts of Scutellaria Radix on the alveolar bone formation in the extract socket of rat. Thirty-six Sprague-dawley rats were used in this study. Mean body weight of rat was $130{\pm}5g$. Experimental animal were administered 0.4% ,${\beta}-aminopropionitril$(Sigma, USA) with the solid commercial food for 5 days. All the maxillary 1st molar of the rats were extracted by using of the tissue forcep under the general anesthesia with Pentobarbital sodium(Tokyo Chemical Co, Japan) injection into intraperitoneal space. All the extracted rats were divided into two group, experimental group which were feeded the solid food mixed ethanolic extracts of Scutellaria Radix, and control group which were feeded same food without reagent. At 1, 3, 5, 7, 9 and 14th days after tooth extraction, rats in both groups were serially sacrificed respectively. All the specimen were treated as usual method and prepared Hematoxylin-eosin stain for the light microscopic observation. The results were as follows : 1. Bone formation of extracted socket starts from the area on remained periodontal ligament than other area. 2. In the case of administration of the extracted Scutellaria Radix showed rapid healing process of connective tissue than non-administrated group. 3. In the case of administration of the extracted Scutellaria Radixshowed rapid osteogenesis than non-administrated With above results, it was concluded that ethanolic extracts of Scutellaria Radix may play a favorable role on the healing process of exatraction socket after extraction in rats. It was suggested that further study to evaluate the different concentration and administration method of ethanolic extracts of the Scutellaria Radix into same experimental model.

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Application and Evaluation of LAMP-PCR for the Diagnosis of Silkworm Pebrine Disease

  • Jong Woo Park;Pu Reun Kook;Jeong Sun Park;Yeong Hee Cho;Seul Ki Park;Hyeok Gyu Kwon;Ji Hae Lee;Sang Kuk Kang;Seong-Wan Kim;Kee Young Kim;Seong-Ryul Kim
    • International Journal of Industrial Entomology and Biomaterials
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    • 제48권3호
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    • pp.139-146
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    • 2024
  • For stable silkworm breeding and high-quality sericulture product production, the detection of Pebrine disease in silkworm eggs is critical. Current diagnostic methods can be timeconsuming and complex. This study aimed to develop a simplified and rapid diagnostic method using loop-mediated isothermal amplification (LAMP) technology to detect pebrine infection in silkworm mother moths. Eight primer candidates targeting the ribosomal gene region of microsporidia were designed and evaluated for specificity and detection sensitivity. A simplified nucleic acid extraction method was established, and isothermal amplification was performed using the selected primers. Of these, primers ID30 and ID45 showed no polymerization, while ID5, ID18, and ID76 exhibited nonspecific reactions, making them unsuitable. Primers ID1, ID6, ID45, and ID82 successfully amplified DNA only in the presence of pebrine, with ID82 demonstrating the best reproducibility and sensitivity, detecting as low as 2.5 pg/ul of DNA through electrophoresis and 5 pg/ul via a colorimetric change with phenol red. The entire process, from nucleic acid extraction to detection, was completed within 60 min. The use of the ID82 primer set in LAMP technology offers a promising and efficient approach for the rapid diagnosis of pebrine disease, potentially enhancing quality control in sericulture.

Application of Buoyant Density Centrifugation Method for the Rapid Detection of Feline Calicivirus in Oyster and Lettuce as Norovirus Surrogate

  • Cho, Yun-Sik;Lee, Kang-Whie;Jang, Keum-Il;Ahn, Jun-Bae;Kim, Kwang-Yup
    • Food Science and Biotechnology
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    • 제17권5호
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    • pp.925-930
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    • 2008
  • Norovirus has become the most common cause of human gastroenteritis in developed countries. Detection procedures of foodborne viruses from foods require several steps. The concentration step using polyethylene glycol (PEG) is time-consuming and the detection efficiency of reverse transcription-polymerase chain reaction (RT-PCR) is affected by inhibitors from food components. In this study, a rapid detection method based on buoyant density centrifugation was developed to replace the time-consuming chloroform-polyethylene glycol-Tris Tween method. Feline calicivirus that belongs to the family Caliciviridae was used as a surrogate model for norovirus. After artificial inoculation of feline calcivirus (FCV) to oyster and lettuce, 830 ${\mu}L$ of homogenized sample suspension was layered on the top of 670 ${\mu}L$ 20% percoll and centrifuged. Then RNA extraction step was proceeded with the supernatant. By varying several physical conditions, the detection limits were lowered to $2.4{\times}10^2$ PFU per 1 g in oyster and $2.4{\times}10^0$ PFU per 1 g in lettuce. The protocol obtained in this study could be used to develop new detection method for norovirus in foods.

면역크로마토그래피를 이용한 토양 및 쌀의 Cd 간이진단법 평가 (Evaluation of a Rapid Immunochromatography Technique for Determination of Cd in Soils and Rice Grains)

  • 이상필;김록영;;김성철;김원일;양재의
    • 한국토양비료학회지
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    • 제45권6호
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    • pp.900-903
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    • 2012
  • Cadmium is known to be very toxic to human health and can be relative easily translocated from soils in plants. Therefore, a rapid method for screening Cd in soils and crops has become more and more important. For this reason, we examined a rapid immunochromatograpy (ICG) test kit which uses antigen-antibody reaction based on immunoassay and chromatography. Soils and rice grains collected from mine waste-contaminated sites were determined for their Cd contents using this kit. For comparison purposes, 0.1 M HCl and ICP-OES were employed as a conventional extraction and determination method. Cadmium contents in rice grains determined using ICG technique were $0.46{\sim}2.39mg\;kg^{-1}$ and Cd contents determined using 0.1 M HCl and ICP-OES were $0.52{\sim}1.97mg\;kg^{-1}$. The correlation between these two Cd contents were statistically significant ($r^2$=0.930). The results of Cd contents in soils also showed a statistically significant relationship between these two methods ($r^2$=0.975). On the basis of these results, ICG technique can be applied to rapidly quantify Cd in crops and soils. However, further research is necessary to apply ICG technique for the field screening.

홍조류인 한국산 김종에서의 염색체 DNA 분리방법 (Rapid and Efficient Purification of Nucleic Acids from the Macroalga Porphyra(Rhodophyta))

  • 류태형;최학선;최경희;이춘환
    • 한국식품영양과학회지
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    • 제27권6호
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    • pp.1166-1172
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    • 1998
  • A method for the isolation and purification of DNA from a red algae, Porphyra was innovated. The innovation of the method consists mainly of three steps that include sodium acetate treatment, chloroform extraction, and 0.2 volume isopropanol precipitation step. The sodium acetate treatment was designed to remove polysaccharide contamination, and the isopropanol step to remove proteins and salts contaminents. Genomic DNA,s of several species(for example, P. tenera, P. yezoensis, P. seriata, and P. pseudolinearis) was successfully isolated by the innovated method. The amount of DNA purified from one g of sample material with the innovated method was 53 g in average. The resulting DNA was characterized to include high molecular weight and showed no nuclease activity. The DNA was pure enough to be digested directly by various restriction enzymes without any difficulties. Porphyra DNA was pure enough and adequate for amplification reaction through the polymerase chain reaction (small nuclear rDNA PCR amplification).

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