• Journal of the Institute of Convergence Signal Processing
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• v.9 no.2
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• pp.121-128
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• 2008

Accurate voice activity detection have a great impact on performance of speech applications including speech recognition, speech coding, and speech communication. In this paper, we propose methods for voice activity detection that can adapt to various car noise situations during driving. Existing voice activity detection used various method such as time energy, frequency energy, zero crossing rate, and spectral entropy that have a weak point of rapid. decline performance in noisy environments. In this paper, the approach is based on existing spectral entropy for VAD that we propose voice activity detection method using MFB(Met-frequency filter banks) spectral entropy, gradient FFT(Fast Fourier Transform) spectral entropy. and gradient MFB spectral entropy. FFT multiplied by Mel-scale is MFB and Mel-scale is non linear scale when human sound perception reflects characteristic of speech. Proposed MFB spectral entropy method clearly improve the ability to discriminate between speech and non-speech for various in noisy car environments that achieves 93.21% accuracy as a result of experiments. Compared to the spectral entropy method, the proposed voice activity detection gives an average improvement in the correct detection rate of more than 3.2%.

• Bulletin of the Korean Chemical Society
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• v.33 no.5
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• pp.1608-1612
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• 2012

Singlet oxygen ($^1O_2$) is an important species for oxidation in biological processes. $^1O_2$ is implicated in the genotoxic effect, and plays an important role in the cell-signaling cascade and in the induction of gene expression. However, the rapid detection of $^1O_2$ in biological environments with sufficient specificity and sensitivity is hampered by its extremely low emission probability. Here, a layer-by-layer (LbL) film of CdTe quantum dots (QDs), polymers, and ascorbate have been designed as a rapid, highly selective, and sensitive fluorescence probe for $^1O_2$ detection. Upon reaction with $^1O_2$, the probe exhibits a strong photoluminescence (PL) response even at trace levels. This remarkable PL change should enable the probe to be used for $^1O_2$ detection in many chemical and biological systems and as an environmental sensor.

• Korean Journal of Veterinary Service
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• v.24 no.4
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• pp.335-341
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• 2001

Swine dysentery caused by Brachyspira hyodysenteriae, an anaerobic, beta-hemolytic spirochete, is a severe mucohemorrhahic diarrheal disease that primarily affects pigs during the growing and finishing period. The current standard laboratory procedure to culture and identify B hyodysenteriae takes 3 to 7 days. This report present a rapid PCR for detection B hyodysenteriae in a single reaction using DNA from swine intestinal samples. The PCR produced a specific 421bp PCR product with template DNA purified from B hyodysenteriae, and the accuracy for detection of B hyodysenteriae by PCR results compared with those of conventional method was 100% in intestinal specimens. Nonspecific bands were not detected with B innocens, a nonpathogenic common inhabitant spirochete, including other enteric bacterial organisms. This procedure could detect as little as 50 pg of template DNA for B hyodysenteriae.

• Journal of Dairy Science and Biotechnology
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• v.31 no.2
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• pp.165-170
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• 2013

Milk and dairy products are not only excellent foods for humans, providing plentiful varied nutrients, but are also a good medium for detrimental food-borne pathogens. Although the food safety field has stabilized due to standardization of food processing, such as the hazard analysis critical control point (HACCP), outbreaks and cases caused by food-borne pathogens still occur at high rates. In approximately 30% of cases, the disease-causing pathogenic organism is undetermined. Recently, a biosensor was developed that has a simple and fast response and overcomes the problems of conventional methods such as cultivation, immuno-assay, polymerase chain reaction, and microarray. Due to the high selectivity and sensitivity of optical biosensors, it is a suitable method for the immediate detection of food-borne pathogens in milk and dairy products.

• Microbiology and Biotechnology Letters
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• v.50 no.4
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• pp.584-591
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• 2022

Identifying carbapenem-resistant Enterobacteriaceae (CRE) is necessary to prevent nosocomial CRE infection outbreaks. Here, a rapid identification method with reduced enrichment time was developed without compromising accuracy. A total of 49 rectal swabs requested for CRE screening at the Department of Diagnostic Medicine at Hospital B in Busan, Korea, were included in this study. Specimens were inoculated on MacConkey and CHROMID Carba media either directly or following enrichment for 3, 6, and 24 h in 100 μl trypticase soy broth containing an ertapenem disk. The enriched cultures were further inoculated on CHROMID Carba or MacConkey media containing an ertapenem disk. In total, 19 CRE and 5 carbapenem-intermediate Enterobacteriaceae isolates were obtained from the 49 swabs. Among the 19 CRE isolates, carbapenemase-producing Enterobacteriaceae constituted 13 strains. Moreover, of the 19 CRE isolates, 16 (81.25%) and 17 (88.24%) were identified from the direct cultures on MacConkey and CHROMID Carba media, respectively. After 3 h of enrichment, the proportions of the CRE identified in the media were: MacConkey medium, 16/19 (81.25%); CHROMID Carba medium, 17/19 (88.24%); and MacConkey medium containing an ertapenem disk, 17/19 (88.24%). The detection rates after 6 h of enrichment were the same for all three media (19/19 strains, 100%), whereas those after 24 h of enrichment were 21, 22, and 24 strains, respectively, but included false positives. These findings suggest that a 6-h enrichment before inoculation on the CHROMID Carba medium is optimal for the rapid and accurate detection of CRE in clinical samples.

• The Journal of the Acoustical Society of Korea
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• v.42 no.4
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• pp.320-328
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• 2023

Underwater active target detection is vital for defense systems, requiring accurate detection and estimation of distance and velocity. Sequential transmission is necessary at each beam angle, but divided pulse length leads to range ambiguity. Multi-frequency transmission results in time-bandwidth product losses when bandwidth is divided. To overcome these problem, we propose a novel method using Generalized Sinusoidal Frequency Modulation (GSFM) for rapid target detection, enabling low-correlation pulses between subpulses without bandwidth division. The proposed method allows for rapid updates of the distance and velocity of target by employing GSFM with minimized pulse length. To evaluate our method, we simulated an underwater environment with reverberation. In the simulation, a linear frequency modulation of 0.05 s caused an average distance estimation error of 50 % and a velocity estimation error of 103 % due to limited frequency band. In contrast, GSFM accurately and quickly tracked targets with distance and velocity estimation errors of 10 % and 14 %, respectively, even with pulses of the same length. Furthermore, GSFM provided approximate azimuth information by transmitting highly orthogonal subpulses for each azimuth.

• Analytical Science and Technology
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• v.21 no.3
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• pp.174-182
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• 2008

The study on the development of rapid analytical method of PCBs containing waste was performed by considering the extraction, column cleanup process, analytical condition and so on. In the established method, new sample clean-up procedure, new quantification peaks and temperature program were introduced. Method detection limit of the method was 0.5 mg/L, and the method could save the total run time to 2/3, therefore save the analysis cost, The new rapid analytical method of transformer oil was suggested to the waste official test method.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.508-513
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• 2008

Erythromycin has been used to treat Streptococosis, Edwardsiel1osis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electro spray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, $87.6{\pm}5.0%$; black rockfish, $87.2{\pm}6.4%$; eel, $85.2{\pm}4.8%$; and rainbow trout, $86.0{\pm}6.2%$. In the established method in this study, the correlation of coefficient values ($R^2$) of erythromycin calibration curve (n=11) was 0.9998.

• Food Engineering Progress
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• v.21 no.3
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• pp.191-200
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• 2017

There have been great efforts to develop a rapid and sensitive detection method to monitor the presence of pathogenic bacteria in food. While a number of methods have been reported for bacterial detection with a detection limit to a single digit, most of them are suitable only for the bacteria in pure culture or buffered solution. On the other hand, foods are composed of highly complicated matrices containing carbohydrate, fat, protein, fibers, and many other components whose composition varies from one food to the other. Furthermore, many components in food interfere with the downstream detection process, which significantly affect the sensitivity and selectivity of the detection. Therefore, isolating and concentrating the target pathogenic bacteria from food matrices are of importance to enhance the detection power of the system. The present review provides an introduction to the representative sample preparation strategies to isolate target pathogenic bacteria from food sample. We further describe the nucleic acid-based detection methods, such as PCR, real-time PCR, NASBA, RCA, LCR, and LAMP. Nucleic acid-based methods are by far the most sensitive and effective for the detection of a low number of target pathogens whose performance is greatly improved by combining with the sample preparation methods.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.387-393
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• 2011

The objective of this study was to develop a real-time PCR method for the rapid detection and quantification of the protozoan pathogen Perkinsus olseni using a TaqMan probe. For the standard, genomic DNA was extracted from $10^5$ in vitro-cultured P. olseni trophozoites, and then 10-fold serial dilutions to the level of a single cell were prepared. To test the reliability of the technique, triplicates of genomic DNA were extracted from $5{\times}10^4$ cells and 10-fold serial dilutions to the level of 5 cells were prepared. The standards and samples were analyzed in duplicate using an $Exicycler^{TM}$ 96 real-time quantitative thermal block. For quantification, the threshold cycle ($C_T$) values of samples were compared with those obtained from standard dilutions. There was a strong linear relationship between the $C_T$ value and the log concentration of cells in the standard ($r^2$ = 0.996). Detection of DNA at a concentration as low as the equivalent of a single cell showed that the assay was sensitive enough to detect a single cell of P. olseni. The estimated number of P. olseni cells was similar to the original cell concentrations, indicating the reliability of P. olseni quantification by real-time PCR. Accordingly, the designed primers and probe may be used for the rapid detection and quantification of P. olseni from clam tissue, environmental water, and sediment samples.