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QTL Analysis of Rice Heading-related Genes Using Cheongcheong/Nagdong Doubled Haploid Genetic Map (청청/낙동 배가반수체 유전자 지도를 이용한 쌀의 출수기 관련 양적형질유전자좌(QTL) 분석)

  • Jang, Yoon-Hee;Park, Jae-Ryoung;Kim, Kyung-Min
    • Journal of Life Science
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    • v.30 no.10
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    • pp.844-850
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    • 2020
  • Disaster-related extreme weather is rapidly increasing due to climate change. In Korea, typhoons accompanied by rainfall usually approach in August and September, causing great damage. The purpose of this study is to find a gene that regulates the heading date of rice in order to avoid loss of harvest from climate change and typhoons. Cheongcheong/Nagdong doubled haploid (CNDH) was used as the plant material to investigate the location of heading-related genes using QTL and sequence analysis by cloning the gene. In the distribution chart, the heading dates, culm lengths, panicle lengths, numbers of panicles, and 1,000-grain weights all have normal distributions. QTL analysis found 13 contigs on chromosome 8. One QTL, named qHd8, was detected on chromosome 8. The range at qHd8 was approximately 7.7 cM, with RM72 and RM404 markers near the peak. There were 13 contigs and 1 ORF. Protein sequence analysis showed that rice was similar to Os08g0341700, AtSFH13, and AtSFH7 proteins. Os08g0341700, which is involved in signal transduction, is similar to phosphatidylinositol transfer-like protein II, and complete information is not available, but it is believed to play a role in the phosphatidylinositol-specific signaling pathway related to Sec14P.

Fabrication of a Ultrathin Ag Film on a Thin Cu Film by Low-Temperature Immersion Plating in an Grycol-Based Solution (글리콜 용매 기반 저온 치환 은도금법으로 형성시킨 동박막 상 극박 두께 Ag 도금층)

  • Kim, Ji Hwan;Cho, Young Hak;Lee, Jong-Hyun
    • Journal of the Microelectronics and Packaging Society
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    • v.21 no.2
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    • pp.79-84
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    • 2014
  • To investigate the plating properties of a diethylene glycol-based Ag immersion plating solution containing citric acid, silver immersion plating was performed in a range from room temperature to $50^{\circ}C$ using sputtered Cu specimens. The used Cu specimens possessed surface structure with large numbers of pinholes which were created with over-acid etching. The Ag immersion plating performed at $40^{\circ}C$ exhibited that the pinholes and copper surface were completely filled with Ag just after 5 min mainly due to galvanic displacement reaction, indicating the best plating properties. Subsequently, the surface morphology of Ag-coated Cu became rougher as the plating time increased to 30 min because of the deposition of silver nanoparticles created by chemical reduction in the solution. The specimen that its overall surface was covered with silver indicated the start of oxidation at temperature higher than around $50^{\circ}C$ in air as compared with pure Cu, indicating enhanced anti-oxidation properties.

Hematologic and biochemical reference intervals for natural monument Korean goral (Naemorhedus caudatus) (천연기념물 한국 산양의 혈액 및 혈액 화학적 정상표준범위)

  • Ahn, Sangjin;Choi, Youngjin;Kim, Jong-Taek
    • Korean Journal of Veterinary Service
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    • v.40 no.2
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    • pp.155-159
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    • 2017
  • Korean goral (Neamorhedus caudatus) is registered as a natural monument number 217 by South Korea Cultural Heritage Administration. It is also recognized as the endangered species I by Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). In this study, blood samples of Korean goral were collected to make reference intervals. Blood sampling was conducted on 19 numbers of Korean gorals (ten females, nine males), which were reared in Korean Goral Restoration Center. For total samples, the reference intervals of hematological parameters were: white blood cell $7.69{\sim}10.87K/{\mu}{\Lambda}$; hematocrit 36.73~46.18%; red blood cell $10.72{\sim}12.86K/{\mu}{\Lambda}$; hemoglobin 12.79~15.14 g/dL; mean corpuscular volume 33.15~36.75 fL; mean corpuscular hemoglobin 11.53~12.23 pg; mean corpuscular hemoglobin concentration 32.64-35.91 g/dL and red blood cell distribution width 39.2~43.46%. For total samples of each parameters, the following results were obtained for serum biochemistry: glucose 111.81~153.77 mg/dL; blood urea nitrogen 22.35~28.91 mg/dL; creatine 1.22~1.84 mg/dL; phosphate 4.57~6 mg/dL; calcium 8.7~9.1 mg/dL; total protein 6.53~6.92 g/dL; albumin 3.1~3.48 g/dL; globulin 3.26~3.62 g/dL; alanine aminotransferase 56.7~158.56 U/L; aspartate aminotransferase 230.35~473.06 U/L; alkaline phosphatase 178.06~332.47 U/L; gamma-glutamyl transpeptidase 131.6-~181.24 U/L; total bilirubin 1.47~2.12 mg/dL; cholesterol 46.48~71.52 mg/dL; amylase 16.3~26.03 U/L; sodium 150.43~153.88 mmol/L; potassium 3.98~4.6 mmol/L and chlorine 109.48~113.26 mmol/L. The ranges of values were similar campared to previous studies except in the case of RDW value, which showed higher range than the RDW value of a previous study. The reference intervals from this study will be useful data for treatment and management of gorals.

The effect of temperature to scuticociliatida Miamiensis avidus proliferation, and to mortality of infected olive flounder Paralichthys olivaceus (온도가 스쿠티카충 Miamiensis avidus의 증식과 넙치에 감염시 폐사에 미치는 영향)

  • Bae, Min-Ji;Im, Eun-Young;Kim, Heung-Yun;Jung, Sung-Ju
    • Journal of fish pathology
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    • v.22 no.2
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    • pp.97-105
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    • 2009
  • Scuticociliates Miamiensis avidus (syn. Philasterides dicentrarchi) causes high mortality and bad growth in olive flounder Paralichthys olivaceus. Temperature is an important factor not only for growth of pathogens but also for host immune system in poikilothermal animal. In this study, temperature affecting ciliate growth and pathogenicity against olive flounder were examined. Doubling time for the ciliate growth was 61.82 hours at $5{^{\circ}C}$, 26.32 hours at $10{^{\circ}C}$, 21.14 hours at $15{^{\circ}C}$, 16.86 hours $20{^{\circ}C}$ and 16.21 hours at $25{^{\circ}C}$. Maximum ciliate numbers were similar at $10-20{^{\circ}C}$ at the range of $1.54-1.75{\times}10^{5}$/ ml. Duplicated intraperitoneal injections were conducted with the ciliates by the concentrations of $1{\times}10^{2}$, $1{\times}10^{6}$, $1{\times}10^{4}$and $1{\times}10^{5}$/ fish (average 8.34 cm, 4.33 g) then kept at $10{^{\circ}C}$, $15{^{\circ}C}$ and $20{^{\circ}C}$. Cumulative mortality was low at $10{^{\circ}C}$ and the mortality was increasing at higher water temperatures. In addition, cumulative mortality was higher at higher dose of infections. In conclusion, Scuticocilite M. avidus grew well at higher temperature (at $5{^{\circ}C}$, $10{^{\circ}C}$, $15{^{\circ}C}$ and $25{^{\circ}C}$) in vitro, and olive flounder mortality due to M. avidus was highly water temperature and dose dependent. The results of this study suggest that water temperature control may one of the essential factor to reduce mortality due to M. avidus infection.

Algorithm for a Minimum Linear Arrangement(MinLA) of Lattice Graph (격자 그래프의 최소선형배열 알고리즘)

  • Sang-Un Lee
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.24 no.2
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    • pp.105-111
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    • 2024
  • This paper deals with the minimum linear arrangement(MinLA) of a lattice graph, to which an approximate algorithm of linear complexity O(n) remains as a viable solution, deriving the optimal MinLA of 31,680 for 33×33 lattice. This paper proposes a partitioning arrangement algorithm of complexity O(1) that delivers exact solution to the minimum linear arrangement. The proposed partitioning arrangement algorithm could be seen as loading boxes into a container. It firstly partitions m rows into r1,r2,r3 and n columns into c1,c2,c3, only to obtain 7 containers. Containers are partitioning with a rule. It finally assigns numbers to vertices in each of the partitioned boxes location-wise so as to obtain the MinLA. Given m,n≥11, the size of boxes C2,C4,C6 is increased by 2 until an increase in the MinLA is detected. This process repeats itself 4 times at maximum given m,n≤100. When tested to lattice in the range of 2≤n≤100, the proposed algorithm has proved its universal applicability to lattices of both m=n and m≠n. It has also obtained optimal results for 33×33 and 100×100 lattices superior to those obtained by existing algorithms. The minimum linear arrangement algorithm proposed in this paper, with its simplicity and outstanding performance, could therefore be also applied to the field of Very Large Scale Integration circuit where m,n are infinitely large.

Soil Environmental Characteristics and the Growth of Young Rice Seedlings (토양환경(土壤環境)의 특성(特性)과 벼 어린모의 생육(生育))

  • Hur, Bong Koo;Jo, In Sang;Um, Ki Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.1
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    • pp.55-60
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    • 1991
  • This study was carried out to obtain the effects of soil environment and characteristics on the growth and yield of young rice seedling. Twenty four rice fields were selected among the labor saving mechanization complex, and the growth statu s and various soil characteristics were investigated The young rice seedling planted soils were located at the range of elevation 5m to 210m, and the average water holding days was 5.1 days, and drainage class were mainly moderately well to imperfectly. The plant height on 30th day after machine transplanting were higher and the tiller numbers were smaller at the higher clay contents at moderately well and imperfectly drained soils. In case of sandy loam soils, the better the drainage class, the higher plant and more tillers. Soil hardness was lower in sandy loam, and the bulk densities of loam, silty clay loam and poorly drained soils were lower than the other fields. There were not differences of soil chemical properties between the soil textural groups but organic matter content were higher in poorly drained soils. The rice yields of young seedling planted fields were negatively correlated to soil bulk density and highly significantly correlated to the exchangeable calcium contents of the soils. The average yield of young rice seedlings was 487kg/10a, 2.5% higher than semi-adult rice seedling of 475kg/10a.

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Chemical Properties of Medicinal Plant Cultivated Soils (약용작물(藥用作物) 재배지(栽培地) 토양(土壤)의 화학성분(化學成分) 함량(含量))

  • Jung, Goo-Bok;Kim, Bok-Young;Kim, Kyu-Sik;Lee, Jong-Sik;Ryu, In-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.1
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    • pp.20-25
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    • 1996
  • This survey was conducted to investigate the chemical properties of soils cultivated medicinal plants. The soils were collected at 254 sites(Angelica gigas : 81, Astragalus membranceus : 38, Platycodn glandiflorum : 36, Paeonia albiflora : 34, Codonopsis lanoceolata : 32, Ligusticum chuanxiong : 17. Bupleurum falcatum : 16, respectively) throughout the country by 0-15cm depth. The chemical properties of soils were pH 5.6, O.M 3.0%. Av.$P_2O_5$ : 405 mg/kg, Ex.K : $0.67cmol^+/kg$, Ex.Ca : $6.3cmol^+/kg$, Ex.mg : $1.6cmol^+/kg$. The percentage distribution of pH on the basis of soil sample numbers were much more at the 5.1-6.0 range than any other ranges, especially those of below pH 6.0 were 83-91% for Angelica gigas, Platycodn glandiflorum and Codonopsis lanoceolata. The distribution of OM and Av.$P_2O_5$ in soils were much at the 2.1-4.0% and above 500mg/kg ranges, respectively. And the content of those in soils cultivated with Platycodn glandiflorum were lower than any other medicinal plants. The distribution of exchangeable cations in soils were much at the 0.2-0.8, 2.1-4.0 and $0.5-2.0cmol^+/kg$ of Ex.K. Ca and mg. respectively, and the contents were higher in soils cultivated Astragalus membranceus. and lower in soils cultivated Platycodn glandiflorum and Codonopsis lanoceolata than any other samples.

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A Study on the Cleanup Process of HOCs-Contaminated Soil by Ex-situ Soil Washing Technology (Ex-situ 토양세척기법에 의한 소수성 유기오염물질로 오염된 토양의 정화에 관한 연구)

  • Choi, Sang-Il;Ryoo, Doo-Hyun;Jang, Min
    • Journal of Korea Soil Environment Society
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    • v.2 no.1
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    • pp.99-107
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    • 1997
  • In this study, a series of batch-scale tests were conducted to optimize the design parameters for the application of soil washing techniques to the hydrophobic organic compounds(HOCs)-contaminated soil and to find the effective methods for the recovery of surfactants from washing effluent by using solvent. Several nonionic surfactants (polyoxyethylene oleyl ester) and sophorolipid were applied to the artificially contaminated soil (4,000 mg n-dodecane/kg dry soil). The effects of washing time, concentration of surfactant solution, dilution ratio, and temperature on washing efficiencies were examined. Hydrophile-liphophile balance (HLB) number was proven to be one of the important parameters for soil washing. The HLB numbers of OA-5 and sophorolipid are too low to form a stable soil-water emulsion. They showed very low washing efficiencies less than 10e1o. If HLB number is in the proper range to form a stable soil-water emulsion, surfactant having higher solubility for HOCs shows higher washing efficiency. OA-14 having higher HLB number than OA-9 formed more stable soil-water emulsion. But its washing efficiency was about 20% due to a lower molar solubility ratio (MSR) than OA-9. OA-9, which forms a stable soil-water emulsion and has comparatively high sotubility for HOCs, showed about 60% washing efficiency by itself. To recover anthracene effectively from OA-9 washing effluent by using benzene as an organic solvent, desirable temperature and pH were $30^{\circ}C$ and 2, respectively.

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Cytologic Aspects of Oral Squamous Cell Carcinoma in a Captive African Hedgehog (Altelerix albiventris) (고슴도치의 구강 편평상피암종의 세포학적 고찰 1증례)

  • Cho, Hyang Mi;Choi, Ul Soo;Lee, Hae Beom
    • Journal of Veterinary Clinics
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    • v.30 no.3
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    • pp.214-217
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    • 2013
  • A 3-year-old intact female hedgehog (Atelerix albiventris) was presented for evaluation of mandibular swelling. Fine needle aspiration of swollen mandibular was performed and smears were stained with Romanowsky type stain for cytological evaluation. Smears were highly cellular with predominance of variably shaped keratinized or non keratinized squamous cells with low numbers of spindloid to abnormally elongated cells. Cytologic impression was squamous cell carcinoma. The mass was surgically removed for histological examination. Microscopically tortuous and anastomosing delicate to broad pegs and nests of neoplastic squamous epithelial cells were supported by a moderate collagenous and spindloid fibroblast stroma. Tumor cells had moderate anisocytosis and mild anisokaryosis and range from moderately to well keratinized, with areas of intratumoral acantholysis accompanied by mixed stromal lymphoplasmacytic, neutrophilic inflammation. The mitotic index is 2-3 per high-power field. Tumor cells were expanding the subcutis subjacent to the layer of skeletal muscle and incorporating the osseous tissue fragments. The final diagnosis was squamous cell carcinoma. The patient survived three months after surgery without any further medical treatments.

Estimating the Impact on Aviation Demand by High Speed Railroad Service in Korea (고속철도 개통으로 인한 항공수요 변화에 대한 추정)

  • Park, Yong-Hwa;Kim, Yeon-Myung;Oh, Sung-Yeol
    • Journal of Korean Society of Transportation
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    • v.22 no.6
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    • pp.47-54
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    • 2004
  • Recently, a large reduction in domestic aviation demand had roughly shown in the range between 34% and 75% in east central and western corridor of Korea. The reasons for the drop in air traffic demand were the provisioning of several new highway serveces and the national economic difficulties. Since April 2004, moreover, the Korea Train Express(KTX) was operated between Seoul and Daegu in the first phrase of 293Km in KTX operational distance and 258Km in air route distance. The operation of KTX significantly impacted air traffic volume, particularly on the overlapped air routes with KTX routes. This study analyzed the effects on air traffic demand in accordance with the opening of KTX by applying the Stated Preference (SP) survey method, the survey conducted prior to 8 months of the KTX initiative. Also, the comparison of the decreased demand forecast by SP analysis and actual revealed traffic volume during two months service after inauguration of KTX was conducted. The Seoul-Daegu route was analysed using the 3 variables considered access and egress time, fare rate, operational frequency. The result obtained from the analysis showed that air users would be preferred only 14%. Comparatively, however, the actual revealed air passengers after the opening KTX were remained 28%, The less "decreased demand" was caused by the instability of the KTX's operation in the initial stage. Therefore, small numbers of passenger were preferable to airservices rather than high speed railroad service.