• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.4
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• pp.565-569
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• 2011

Validation of quercetin as a marker compound in the standardization of Changnyeong onion extract developed for functional health food was attempted by analytical method. The specificity was satisfied with retention time and photo diode array (PDA) spectrum by analysis of quercetin using HPLC and comparison with standard compound. It showed a high linearity in the calibration curve as coefficient of correlation ($R^2$) of 0.9986, and the limit of detection (LOD) and limit of quantitation (LOQ) were 0.2 mg/L and 0.5 mg/L, respectively. Recovery rate test with quercetin concentration of 0.05, 0.075 and 0.1 mg/mL was revealed in the high range of 82.36~95.26%, 82.70~98.24% and 87.91~95.11%, respectively. The intra-day and inter-day precision in quercetin for Changnyeong onion extracts was 0.10~3.28% and 0.96~5.79%, respectively. Therefore, application of quercetin was validated in analytical method as a marker compound in Changnyeong onion extracts.

• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.52-57
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• 2019

Method development and validation of decursin for the standardization of Angelica gigas Nakai as a functional ingredient and health food were accomplished. The quantitative determination method of decursin as a marker compound of aerial parts of Angelica gigas Nakai extract (AAGE) was optimized by HPLC analysis using a C18 column ($3{\times}150mm$, $3{\mu}m$) with 0.1% TFA in water and acetonitrile as the mobile phase at a flow rate of 0.5 mL/min and detection wavelength of 330 nm. The HPLC/PDA method was applied successfully to quantification of the marker compound in AAGE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation ($R^2$) of 0.9994 and the limit of detection and limit of quantitation were $0.011{\mu}g/mL$ and $0.033{\mu}g/mL$, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 1.10% and 1.13%, respectively. Recovery of decursin at 0.5, 1, 5 and $10{\mu}g/mL$ were 92.38 ~ 104.11%. These results suggest that the developed HPLC method is very useful for the determination of marker compound in AAGE to develop a health functional material.

• Journal of Food Hygiene and Safety
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• v.34 no.4
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• pp.334-339
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• 2019

A procedure based on high-performance liquid chromatography (HPLC) is described to determine ${\beta}-carotene$ in infant formulas. The method for ${\beta}-carotene$ analysis was performed on a C18 reversed-phase column using acetonitrile/methanol/dichloromethane (6:1:3, v/v/v) as a mobile phase. ${\beta}-Carotene$ was determined in HPLC with photo diode array (PDA) detector. The parameters of validation were specificity, linearity, LOD, LOQ, accuracy, precision and repeatability. The specificity was confirmed by the retention time and the linearity ($R^2$), which was over 0.999 in the range of 0.125~2 mg/L. The detection and quantification limits were 0.064 and 0.193 mg/L, respectively. The accuracy and precision of this method using an STD spiked sample were 80~119% and 1.02~2.05% respectively. The method was applied to the analysis of various infant formula and follow-up formulas products containing ${\beta}-carotene$, and all the products contained acceptable levels of ${\beta}-carotene$ for nutrition labeling.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.867-873
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• 2023

In this study, for the purpose of standardized quality control of a cold medicine, we simultaneous analyzed four main chemical components of a cold medicine: acetaminophen, caffeine, methyl paraben, and propyl paraben. The sample was subjected to quantitative analysis using high performance liquid chromatography (HPLC), after pretreatment of four components. The experiment was carried out by using Isocratic elution at wavelength of 270nm. Acetonitrile and water (H₂O) were used as a mobile phase at a flow rate of 1.0mL/min in a commercial C18 reversed-phase column. A volume of 10uL cold medicine were injected into the column with column oven temperature at 35℃. As a result of the experiment, the values of Resolution were 4.983, 1.596, 5.519, and 1.678 respectively-well over Rs >1.5, which indicates that the separation of four components were efficient. In addition, value of symmetry factor of the components was 1.056, 1.069, 1.032, and 1.133 respectively, to show its symmetrical stability. The calibration curve of all four components exhibits good linearity with R² >0.9995 to 0.9999. Furthermore, the limit of detection(LOD) were between 0.0118 to 1.5973 mg/mL, while the limit of quantification (LOQ) were between 0.0353 to 4.7919 ㎍/mL with the recovery rate of 79.6% ~ 120.5%. The results of this study showed an efficient quality evaluation of a simultaneous analysis method for cold medicine components.

• Korean Journal of Food Science and Technology
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• v.51 no.4
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• pp.316-323
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• 2019

It is difficult to analyze pymetrozine in citrus fruits using the hydromatrix method because of its low efficiency of purification and overlap of matrix and pymetrozine peaks. Liquid-liquid extraction can analyze pymetrozine in citrus fruits using dichloromethane. Since low pH interferes with the extraction of pymetrozine, the extracts of citrus fruits were maintained over pH 7.0 by adding borax buffer and 1 N NaOH in the improved method. According to the improved method, citrus fruits (such as lemon, lime, orange, tangerine, and grapefruit) were extracted and purified for HPLC-photo diode array analysis. The results of validation were as follows: $4.360{\mu}g/kg$ of limit of detection, $14.533{\mu}g/kg$ of limit of quantitation, and 0.007 mg/kg of method quantitative limit. Citrus fruits spiked with pymetrozine showed a recovery range from 71.8 to 83.7% and a coefficient of variation below 6%. Thus, the improved method can efficiently analyze pymetrozine in citrus fruits.

• The Korean Journal of Pesticide Science
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• v.19 no.1
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• pp.71-79
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• 2015

In order to investigating the effects of antifungal activity of intestinal bacteria obtained from insect, it was identified these bacteria isolated from the gut. In this result, total 49 isolates of intestinal bacteria were identified from 10 kinds of insect species. It was that 4 isolates including Cedecea sp. from Nesidiocoris tenuis, 3 isolates including Enterobacter sp. from Odontotaenius disjunctus, 4 isolates including Acinetobacter sp. from Reticulitermes speratus, 4 isolates including Clavibacter sp. from Riptortus clavatus, 11 isolates including Bacillus sp. from Lema decempunctata, 3 isolates including Enterococcus sp. from Henosepilachna vigintioctopunctata 2 isolates including Staphylococccus sp. from Harmonia axyridis, 5 isolates including Enterobacter asburiae from Popillia mutans, 7 isolates including Aeromonas sp. from Hydrophilus acuminatus, and 7 isolates including Brucella sp. from Anomala octiescostata. In order to investigating antifungal activity against plant-pathogenic fungi, Altanaria solani, Colletotrichum gloeosporioides, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici, Rhizoctonia solani and Selerotinia sclerotiorum were dual cultured with each 49 gut enterobacteriaceae. As these results showed that many isolates have the antifungal activities including 26 isolates against A. solani, 6 isolates against B. cinerea, 13 isolates against C. gloeosporioides, 11 isolates against F. oxysporum, 17 isolates P. capsici, 2 isolates against R. solani and 2 isolates against S. sclerotiorum. Pseudomonas aeruginosa was showed strong antifungal activity against all of tested plant pathogens. It might be taken a potential for application against plant-pathogenic fungi with useful control agent.

• Journal of The Korean Association of Information Education
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• v.9 no.3
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• pp.533-548
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• 2005

According to the increasing of the internet infrastructure and growth of contents technology, the users can access an education service at anytime and anywhere. In a field of English education, especially, internet technology has enabled learners to communicate with their teachers and multimedia contents technology has been able to provide learner not only lots of interests, but also good effect on English learning. In this paper, we propose a method diagnosing learner's level by using some question-items, which consist of item's type and item's function. Futhermore, the proposed system can support three devices which are PC, PDA, and Mobile Phone on wired and wireless internet communication environments. Also, the system provides multimedia contents including flash movies, image, and audio contents. We have applied the system into real classroom, and we verified that the question-items that system provides has no problem, and our method could improve each learner skill on English education. Therefore, we expect that learner who uses the proposed system will get level-based English learning service at anytime and anywhere.

• Research in Plant Disease
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• v.23 no.4
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• pp.322-333
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• 2017

In June and July 2015 and 2017, typical signs and symptoms of brown rot were observed on the fruit of Japanese apricot, peach, apricot, Japanese plum, and sweet cherry with incidence levels of 2-5% in Jeonju and Imsil, Korea. Early symptoms were small, circular, light brown spots that eventually destroyed entire fruit. Small sporodochia later appeared on the surface. Conidia isolated from each host were one-celled, hyaline, lemon-shaped and borne in branched monilioid chains. The optimal temperature range for hyphal growth of all the isolates was $20-25^{\circ}C$. The growth of hyphae was faster on potato dextrose agar and oatmeal agar than others. Multiple alignments using the ITS sequences from different host showed that they matched each other (100%). The ITS sequences showed 100% identity to those of M. fructicola. Based on the morphological characteristics and phylogenetic analysis via internal transcribed spacer (ITS), all the isolate was identified as M. fructicola. Pathogenicity of representative isolates was proved by artificial inoculation, fulfilling Koch's postulates. This is the first confirmed report on brown rot caused by M. fructicola on stone fruit in Korea.

• The Magazine of the IEIE
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• v.28 no.7
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• pp.49-55
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• 2001

1970년대에 개발된 마이크로 프로세서는 제어기기 분야 및 소형 컴퓨터에서 주로 사용되어 오다가 1980년대에 이르러 RISC(Reduced Instruction Set Computer) 구조의 도입으로 중대형 컴퓨터에 이르기까지 광범위하게 사용되고 있다. 또한 반도체 기술의 급격한 발전으로 슈퍼스칼라 구조가 마이크로 프로세서에서도 적용되고 있으며 동작 속도도 수백 MHz에 이르고 있다. 마이크로 프로세서는 프로그램을 수행하기 위해서 프로그램과 데이터를 메모리로부터 읽어 와야 한다. 그런데 메모리 용량은 빠른 속도로 증가하고 있지만 동작 속도는 마이크로 프로세서의 동작 속도에 크게 미치지 못하고 있다. 1980년에 DRAM의 접근 속도는 250nsec이었으나 1998년에 RDRAM의 동작속도는 300MHz로 70여배 빨라졌다. 그러나 마이크로프로세서는 1980년에 8086의 동작 속도가 8MHz이던 것이 1998년에는 팬티엄-2가 500MHz에 이르고 있다. 더욱이 팬티엄-2는 슈퍼스칼라 구조이므로 이를 감안하면 1GHz 이상에 이르러 120여 배 빨라진 것을 알 수 있다. 이와 같은 메모리 속도와 마이크로 프로세서 속도 차이에 더하여, 메모리와 마이크로 프로세서를 인쇄 회로 기판에서 연결하는데 따른 물리적 특성은 변화하지 않으므로 데이터 전송 폭을 넓히는 것에는 한계가 있다. 따라서 향후 컴퓨터 성능 발달을 제한하는 주요 요소 중 하나는 마이크로 프로세서와 메모리 사이의 데이터 전송 폭이다. 프로그램과 데이터가 메모리에 저장되는 본 뉴먼 방식의 컴퓨터에서 데이터 전송 폭을 줄이기 위해서는 코드 밀도(Code Density)가 높은 컴퓨터 구조를 연구하는 것이 필요하다. 한편 마이크로 프로세서는 실장 제어용으로 거의 모든 전자 제품 및 자동화 기기에서 채용하고 있다. 특히 냉장고, 에어콘, 전축, TV, 세탁기 등 가전기기와 Fax, 복사기, 프린터 등 사무용기기와 자동차, 선박, 자동화기계 등 사무 및 산업용 기기와 PDA(휴대용 정보 기기), NC(Network Computer) 등 정보 기기 그리고 각종 오락기, 노래 반주지 등 정보 기기 등에서 사용하는 실장 제어용 마이크로 프로세서 시장은 매년 10% 이상씩 성장하고 있으며, 21세기 산업을 주도하는 핵심 기술로 자리 매김하고 있다. 이러한 실장 제어용 기기는 마이크로 프로세서와 메모리 및 입출력 자이가 하나의 반도체에 집적되는 경우가 많다. 그런데 반도체 가격은 반도체 크기에 따라 결정되며, 가장 넓은 면적을 차지하는 것은 메모리이다. 따라서 반도체 가격을 낮추기 위해서는 메모리 크기를 줄여야 하며, 이를 위해서 또한 코드 밀도가 높은 컴퓨터 구조에 대한 연구가 필요하다. 최근에는 322비트 RISC 명령어를 16비트 명령어로 축약한 구조가 연구되었다. ARM-7TDMI는 ARM-7의 16비트 축약 명령어 구조이며, TR4101은 MIPS-R3000의 16비트 축약 명령어 구조이다. 이들 16비트 축약 명령어 RISC는 종래 RISC와의 호환성을 위하여 2가지 모드로 동작하므로 구조가 복잡하고, 16비트 명령어에서는 8개의 레지스타만을 접근할 수 있으므로 성능이 크게 떨어지는 단점을 가진다.

• Journal of Mushroom
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• v.9 no.1
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• pp.22-26
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• 2011

We bred a new strain of Pleurotus eryngii. It's name is 'Gongi No.3' and it was bred by mating monokaryotic strain isolated from E08-5D2 and dikaryotic strain GMPE25016 from 2006 to 2010 in Mushroom Research Station, Gyonggi province A.R.E.S. The characteristics of a new strain 'Gongi No.3' is as follows ; The optimum temperature for mycelium growth was from 26 to 29 degrees celsius on PDA medium and those for the premodium formation and the growth of fruit body were from 14 to 18 degrees celsius. The period of spawn running was around 30days at 22 degrees celsius and the period taken from scratching old spawn to make premodium were 8 days. The color degree of cap surface was measured by color difference meter and that of a new strain 'Gongi No.3' was 54.4 by L-value. it was seem to be dark, compared with 'Keunneutari No.2'. The hardness of fruit body of a new strain was higher than 'Keunneutari No.2'. The yield was about 180g per bottle(1100cc). it was 10g more than 'Keunneutari No.2'.