• Journal of Ginseng Research
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• v.36 no.1
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• pp.93-101
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• 2012

This study evaluated the anti-cholinesterases (ChEs) and antioxidant activities of white ginseng (WG) and black ginseng (BG) roots of Panax ginseng (PG), P. quinquefolium (PQ), and P. notoginseng (PN). Ginsenosides $Rg_1$, Re, Rf, $Rb_1$, Rc, $Rb_2$, and Rd were found in white PG, whereas Rf was not found in white PQ and Rf, Rc, and $Rb_2$ were not detected in white PN. The major ginsenoside content in steamed BG including $RK_3$, $Rh_4$, and 20(S)/(R)-$Rg_3$ was equivalent to approximately 70% of the total ginsenoside content. The WG and BG inhibited acetylcholinesteras (AChE) and butyrylcholinesterase (BChE) in a dose dependent manner. The efficacy of BG roots of PG, PQ, and PN on AChE and BChE inhibition was greater than that of the respective WG roots. The total phenolic contents and 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) scavenging activity were increased by heat treatment. Among the three WG and BG, white PG and steamed black PQ have significantly higher contents of phenolic compounds. The best results for the DPPH scavenging activity were obtained with the WG and BG from PG. These results demonstrate that the steamed BG roots of the three studied ginseng species have both high ChEs inhibition capacity and antioxidant activity.

• Journal of Ginseng Research
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• v.21 no.1
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• pp.43-48
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• 1997

Dynamic parameters of biochemistry including the POD (peroxidase) activity, contents of DNA and RNA, isozymes of POD and ES (esterase) in American send (Panax quinquefolium L.) seed are reported in the present paper. The dynamic changes of POD activity proved that the PAS (physiological afterripening stage) is a stage in which some substances are prepared for seed germination. The POD activity correlated with ER (embryo ratio) significantly DNA content changed little only within 0.0036∼0.013 mg/ml, which did not correlate with ER. RNA content changed from 0.1539 to 1.0313 mg/ml and correlated significantly with RE during all of the embryo afterripening. None of the POD isozyme band was obtained in ESGS (embryo slowly growth stage), but five bands in ERGS (embryo rapidly growth stage) and six bands in PAS. Four bands of ES isozymes were obtained in ESGS, but six bands in ERGS, particularly, the content of ES isozymes increased in PAS. All of these may provide some information for understanding the dormancy mechanisms of American song seed.

• Korean Journal of Medicinal Crop Science
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• v.25 no.6
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• pp.389-396
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• 2017

Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers. Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background. Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.

• Journal of Ginseng Research
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• v.21 no.3
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• pp.209-213
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• 1997

Sesquiterpenes of Korean ginseng (Panax ginseng C.A. Meyer, KG) and American ginseng (Panax quinquefolium, AG) were Isolated by simultaneous distillation and extraction (SDE) with 30% methanol and n-hexane or adsorption using C18 Sep-Pak . The sesquiterpenes in KG and AG were compared each other by using TLC and GC/MS. Thin layer chromatogram of KG gave 4~5 spots of terpenes colored by vanillin-sulfuric acid, while AG did one major spot. Total ion chromatogram of KG showed about 30 peaks of sesquiterpene having molecular weight 204. Among these, 9 components such as alloaromadendrene, germacrene B, isocaryophyllene, $\alpha$-neoclovene, ${\gamma}$-muurolene and $\beta$-panasinsene together occupied 81.5% of total sesquiterpenes identified. In AG, however, only 3 components, i.e., isocaryophyllene, $\beta$-bisabolene, $\beta$-sesquiphellandrene occupied 79.3% of total sesquiterpenes identified. Especially isocaryophyllene was a dominant component of AG occupying 56.6%. Eight sesquiterpenes, including alloaromadendrene, germacrene B and $\alpha$-humulene, were not detected in AG. These results indicate that sesquiterpenes could be used as indices for the chemical difference between KG and AG.

• Korean Journal of Pharmacognosy
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• v.5 no.2
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• pp.103-109
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• 1974

미국인삼(美國人蔘) (오가과(五加科), Panax quinquefolium L.) 2년생(年生) 및 4년생(年生) 식물(植物)에 심지법(法)을 사용(使用)하여 $squalene-H^3$를 투여(投與)했을 때 미국인삼(美國人蔘) 사포닌(panaquilins)과 그 비당체(非糖禮)에 $H^3-incorporation$ 가능성(可能性) 여부(與否)를 검토 하였다. $squalene-H^3$ 섭취율은 매우 낮은 값을 보였으며 $(40{\sim}86%)$, $squalene-H^3$는 또한 panaquilin의 비당체(非糖體) 부분(部分)에는 incorporation 되지는 않았으나 당체(糖體)에는 squalene 대사물질(代謝物質)이 incorporation된 것으로 사료(思料) 되었다.

• Proceedings of the Ginseng society Conference
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• 1974.09a
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• pp.77-93
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• 1974

The sapogenins of two-and four-year-old A-merican ginseng plants (Panax quinquefolium L.) (Araliaceae) collected in July and September were studied. American ginseng saponins (panaquilins) differ from Korean ginseng (Panax ginseng C. A. Meyer) saponins (ginsenosides). The American ginseng saponins separated and named were panaquilins A, B, C, D, E-l, E-2, E-3, G-l, G-2, (c) and (d). One-dimensional thin-layer chromatography did not completely separate panaquilin mixture and were subject to misinterpretation. The panaquilins were more accurately separated and identified by the two-dimensional thin-layer method established. Some differences in American ginseng saponins were dependent upon the plant age, time of collection, and part extracted. The American ginseng sapogenin components are panxadiol (panaquilins B and C), oleanolic acid (panaquilin D) and panaxatriol (panaquilin G-l). The panaquilins E-l, E-2 and E-3 mixture contains both panaxadiol and panaxatriol. The genins of panaquilins A, (c), (d) and G-2 were not identified. In addition, ${\beta}-sitosterol$ and stigmasterol were identified from the root ether extracts.

• Journal of Ginseng Research
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• v.6 no.2
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• pp.138-142
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• 1982

The composition and concentration of ginsenosides and the free sugars in panax ginseng(Korea ginseng), panax quinquefolium (American ginseng) and panax pseudoginseng var. notoginseng (Sanchi ginseng), were investigated. The major ginsenosides and the order of their amount in panax ginseng are Rbl, Rc Rgl, Re, Rb2 Rd and these are about 90% of total ginsenosides, but major ginsenosides of American and Snachi ginseng art Rbl, Re, Rg1 (about 91% of total) ansi Rgl, Rbl, Re (about 93% of total) respectively. Sanchi ginseng was observed in higher concentration of panaxatriol than panaxadiol unlike panax and American ginseng. Free sugars in white ginseng are fructose, glucose, maltose and sucrose. Whereas, in red ginseng rhamnose and xylose were also detected as free sugar.

• Journal of Ginseng Research
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• v.22 no.2
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• pp.91-95
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• 1998

The distribution of phenolic components of Korean ginseng (Panax ginseng KG) and American ginseng (Panax quinquefolium, AG) were compared by thin-layer chromatography (TLC). Silica gel TLC gave 3~4 spots, while $NH_2$ HPTLC 5~6 spots, which were colored by both $FeCl_3$/$K_3Fe(CN)_6$ and Folin-Ciocalteu. The distribution of phenolic components was quite different between KG and AG. Especially, a polyphenol (m.w. 578), which had been isolated from KG by the author, was not found in AG. This result suggests that the polyphenol could be used as an index compound for the differentiation of KG from AG.

• Plant Resources
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• v.6 no.3
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• pp.165-169
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• 2003

American ginseng (Panax quinquefolium) is herbaceous perennial plants indigenous to North American forests. This is highly valued as medicinal herbs with a long history of collection from wild populations since 1716. Wild American ginseng distributed from Quebec in Canada to northern Florida in USA. A heavy concentration is found in the Appalachian mountains, although wild American ginseng is considered endangered. The price paid for field cultivated ginseng has dropped dramatically in the past 10 years, while the price for wild or woods cultivated ginseng has rised significantly. The price curve for ginseng resembles a roller coaster, reflecting not only supply and demand but many other factors. This information will be useful to understand American ginseng compared to Korean ginseng.

• Journal of Ginseng Research
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• v.12 no.1
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• pp.53-62
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• 1988

A field experiment was conducted in the arid interior of British Columbia, Canada to assess the seasonal soil temperature and moisture regimes in an American ginseng garden. As a consequence of the man-modified microclimate (elevated shade canopy and surface covering of mulch), the growing environment of the crop was fundamentally altered when compared to adjacent agricultural growing environments. In the ginseng garden, soil temperatures were found to remain low throughout the growing season whereas soil moisture remained high when compared with the outside garden environment. These results indicate that even in the hot, arid environment of the interior of British Columbia, the growing of ginseng is undertaken in sub-optimal conditions for the major part of the growing season. This poses challenges for the producers of the crop to modify the architecture of the gardens to enhance the soil regime without creating a deleterious aerial environment.