• 생물환경조절학회지
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• 제20권1호
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• pp.14-20
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• 2011

지역별로 자생하고 있는 백리향 지상부의 휘발성 향기 성분 및 방부성 물질인 phenols의 성분을 조사하기 위해 제주도 고산종, 제주도 중간산종, 경기도, 울릉도, 강원도 서식종을 TD-GC-MSD로 분석한 결과 제주도 중간산종에서 상대적으로 높은 62종의 물질이 조사되었으며 함량도 $7365.22{\mu}g/m^3$으로 높은 농도를 나타냈다. 또한 백리향의 휘발성 향기성분 중 제주도 중간산종이 다른 조사지역보다 상대적으로 thymol과 carvacrol의 phenol성 물질이 다른 휘발성분에 비해 35.92%로 높은 결과를 보였다.

• Journal of Gastric Cancer
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• 제22권4호
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• pp.319-338
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• 2022

Purpose: Gastric cancer (GC) has high morbidity and mortality, the cure rate of surgical treatment and drug chemotherapy is not ideal. Therefore, development of new treatment strategies is necessary. We aimed to identify the mechanism underlying Sp1 regulation of GC progression. Methods and Methods: The levels of Sp1, β-catenin, SET domain bifurcated 1 (SETDB1), and 15-hydroxyprostaglandin dehydrogenase (HPGD) were detected by quantitative reverse transcription polymerase chain reaction and western blot analysis. The targets of SETDB1 were predicted by AnimalTFDB, and dual-luciferase reporter assay was used for confirming the combination of Sp1, β-catenin, and SETDB1. HGC27 or AGS cells (1×10⁶ cells/mouse) were injected into mice via the caudal vein for GC model establishment. The level of Ki67 was detected using immunohistochemistry, and hematoxylin and eosin staining was performed for evaluating tumor metastasis in mice with GC. Results: HPGD was inhibited, while the protein levels of Sp1, β-catenin, and SETDB1 were up-regulated in GC tissues and cell lines. HPGD overexpression or SETDB1 silencing inhibited the proliferation, invasion, and migration of GC cells, and Sp1 regulated the proliferation, invasion, and migration of GC cells in a β-catenin-dependent manner. Furthermore, HPGD served as a target of SETDB1, and it was negatively regulated by SETDB1; additionally, Sp1 and β-catenin bound to the SETDB1 promoter and negatively regulated HPGD expression. We proved that Sp1 regulated GC progression via the SETDB1/HPGD axis. Conclusions: Our findings revealed that Sp1 transcriptionally inhibited HPGD via SETDB1 in a β-catenin-dependent manner and promoted the proliferation and metastasis of GC cells.

• The Korean Journal of Physiology and Pharmacology
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• 제27권3호
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• pp.197-208
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• 2023

Dysregulation of certain long non-coding RNAs may facilitate tumor initiation and progression. However, numerous carcinogenesis-related long noncoding RNAs have not been characterized. The goal of this study was to elucidate the role of LINC00562 in gastric cancer (GC). The expression of LINC00562 was analyzed using real-time quantitative PCR and Western blotting. The proliferative capacity of GC cells was determined using Cell Counting Kit-8 and colony-formation assays. The migration of GC cells were evaluated using wound-healing assays. The apoptosis of GC cells was assessed by measuring the expression levels of apoptosis-related proteins (Bax and Bcl-2). Xenograft models in nude mice were constructed for in vivo functional analysis of LINC00562. The binding relationship between miR-4636 and LINC00562 or adaptor protein complex 1 sigma 3 (AP1S3), obtained from public databases, was confirmed using dual-luciferase and RNA-binding protein immunoprecipitation experiments. LINC00562 was expressed in GC cells at high levels. Knockdown of LINC00562 repressed GC cell growth and migration, promoted apoptosis in vitro, and inhibited tumor growth in nude mouse models. LINC00562 directly targeted miR-4636, and miR-4636 depletion restored the GC cell behavior inhibited by LINC00562 absence. AP1S3, an oncogene, binds to miR-4636. MiR-4636 downregulation increased AP1S3 level, restoring GC cell malignant behaviors inhibited by AP1S3 downregulation. Thus, LINC00562 exerts carcinogenic effects on GC development by targeting miR-4636-mediated AP1S3 signaling.

• Archives of Pharmacal Research
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• 제11권2호
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• pp.99-113
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• 1988

Quantitation of ethenzamide, isopropylantipyrine and caffeine takes about 41 hrs by conventional GC method. Quantitation of allylisoprorylacetylurea takes about 40 hrs by conventional UV method. But quantitation of them takes about 6 hrs by DRIFT developing method. Each standard and sample sieved, powdered and acquired DRIFT spectrum. Out of them peak of each component was selected and ratio of each peak to standard peak was acquired, and then linear stepwise multiple regression was performed with these data and concentration. Reflectance value, Kubelka-Munk equation and Inverse-Kubelka-Munk equation were modified by us. Inverse-Kubelka-Munk equation completed the deficit of Kubelka-Munk equation. Correlation coefficients acquired by conventioanl GC and UV against DRIFT were more than 0.95.

• 약학회지
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• 제52권6호
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• pp.480-487
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• 2008

We described an estimation of measurement uncertainty in quantitative analysis of 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THCCOOH), the major metabolite of ${\Delta}^9$-tetrahydrocannabinol, in urine sample by solid-phase extraction (SPE) and GC/MS detection. The analytical results were compared and the different contributions to the uncertainty were evaluated. Inter-day and inter-person validation were performed using statistical analysis of several indicative factors. Measurement uncertainty associated with target analyte in real forensic samples was estimated using quality control (QC) data. Traceability of measurement was established through traceable standards, calibrated volumetric glassware and volume measuring device. The major factors of contribution to combined standard uncertainty, were calibration linearity, inter-day repeatability and inter-person reproducibility, while those associated with preparation of analytical standards and sampling volume were not so important considering the degree of contribution. Relative combined standard uncertainties associated with the described method was 12.05% for THCCOOH.

• Journal of Applied Biological Chemistry
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• 제57권3호
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• pp.211-218
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• 2014

Polymethoxyflavones (PMFs) are a group of polymethoxylated bioactive flavones with diverse biological activities, including anticancer, anti-inflammatory, antibacterial, and antiviral activities. PMFs are found from various plants such as orange, tangerine, and krachaidum. To establish the simple quantitative analytical methods for PMFs, chromatographic analysis was applied to the selected krachaidum foods because krachaidum contains diverse PMFs compared to other PMF-containing foods. Krachaidum is the rhizome of Kaempferia parviflora, and many commercial krachaidum products, such as tea, juice and wine, are commercially available and consumed as health functional foods in Asian countries. Apart from the claimed health promoting benefits, reliable quality assurance and legal guideline for the registration of these products are not available yet. Twelve PMFs were analyzed from the commercial krachaidum foods by GC-FID and HPLC-DAD. No single chromatographic method could not analyze 12 PMFs simultaneously. HPLC-DAD method was found more sensitive to detect PMFs. Based on our analysis data, we proposed 5,7-dimethoxyflaone and 5,7,4'-trimethoxyflavone as index components for the food products.

• 대한화학회지
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• 제38권10호
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• pp.726-733
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• 1994

대기부유분진(air particulate material)중에 존재하는 다환방향족탄화수소(polycyclic aromatic hydrocarbons, PAH)를 신속하고 정확하게 분석하기 위하여 대기부유분진 시료를 10ml의 초임계유체($N_2O$ )로 30분간 추출 후 별도의 전처리와 농축과정 없이 GC/MS에서 분석하여 분석시간과 분석과정을 단축 및 단순화하였다. 시료로서 NBS 대기부유분진 인증표준기준물질(certified particulate reference material, CRM)1649와 서울의 도심에서 채취한 대기부유분진 시료를 이용하여 기존의 추출법 및 분석방법과 비교하였다. 그 결과 본 분석방법은 기존의 분석법에 비해 회수율은 상대적으로 작았으나 재현성이 좋았으며 분석과정이 간단하고 분석시간이 현저히 단축됨을 알 수 있었다.

• 한국환경농학회지
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• 제26권4호
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• pp.337-342
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• 2007

본 연구에서는 경북의 풍기와 상주에 있는 인삼재배포장에서 직접 시료를 채취하고, 이를 이용한 홍삼농축액제품을 제조하였다. 수삼과 홍삼농축액 중 유기인계와 유기염소계 농약을 GC-NPD와 GC-ECD로 각각 정량분석 하였으며 GC-MSD로 정성분석을 실시하여, 식품의약품안전청이 2007년 1월1일부터 시행한 농약잔류허용기준에 따라 안전성을 평가하였다. 풍기 수삼에서 유기인계 농약은diazinon을 비롯한pyrimethanil, tolclofos-methyl, metalaxyl, diethofencarb, parathion, cyprodinil, tolylfluanid와 kresoxim-methyl이 모두 불검출 되었다. 그러나 상주 수삼에서는 tolclofos- methyl이 평균 $0.054{\pm}0.008\;mg/kg$ 검출되어 수삼의 농약잔류허용기준 0.3mg/kg의 $18{\pm}2%$ 수준이었다. 풍기와 상주 수삼에서 유기염소계 농약은 BHC, DDT의 이성질체와 aldrin, azoxystrobin, captan, cypermethrin, deltamethrin, dieldrin, difenoconazole, endosulfan-sulfate, endrin, fenhexamid, quintozene, ${\alpha}-endosulfan$, ${\beta}$-endosulfan도 검출 되지 않았다. 한편 풍기와 영주의 인삼으로 제조한 홍삼농축액 중에도 유기인계와 유기염소계 농약은 모두 검출 되지 않았다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제30권5호
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• pp.417-427
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• 2008

The aim of this study is to investigate the effect of anodizing surface to osseointegration of implant by using of resonance frequency analysis (RFA), quantitative and qualitative assessment of an anodically modified implant type with regard to osseous healing qualities. A total of 96 screw-shaped implants were prepared for this study. 72 implants were prepared by electrochemical oxidation with different ways. 24 (group 1 SP) were prepared at galvanostatic mode in 0.25M sulfuric acid and phosphoric acid. 24 (group 2GC) were prepared at galvanostatic mode in calcium glycerophosphate and calcium acetate and 24 (group 3 CMP (Calcium Metaphosphate) Coating were prepared at galvanostatic mode in 0.25M sulfuric acid and phosphoric acid followed by CMP coating. Rest of 24 (control group were as a control group of RBM surface. Bone tissue responses were evaluated by resonance frequency analysis (RFA) that were undertaken at 2, 4 and 6 weeks after implant placement in the mandible of mini-pig. Group 1 SP (anodized with sulfuric acid and phosphoric acid implants) demonstrated slightly stronger bone responses than control Group RBM. Group 2 GC (anodized surface with calcium glycerophosphate and calcium acetate implants) demonstrated no difference which were compared with control group. Group 3 GMP (anodized and CMP coated implants) demonstrated slightly stronger and faster bone responses than any other implants. But, all observation result of RF A showed no significant differences between experimental groups with various surface type. Histomorphometric evaluation demonstrated significantly higher bone-to-implant contact for group 2 GC. Significantly more bone formation was found inside threaded area for group 2 GC. It was concluded that group 2 GC (anodized surface with calcium glycerophosphate and calcium acetate implants) showed more effects on the bone tissue responses than RBM surface in initial period of implantation. In addition, CMP showed a tendency to promote bone tissue responses.

• 한국식품과학회지
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• 제32권1호
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• pp.17-24
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• 2000

본 연구에서는 날로 그 생산 및 소비량이 중대 되어가는 추세에 있는 포도주의 품질향상을 이루고자 역삼투 처리기술을 이에 적용하여 시도하였는데, 포도주의 제조공정에서 필요로하는 보당과정 없이 역삼투 처리에 의해 포도 착즙액중의 순수(純水)를 제거함으로써 자체 당도를 농인 상태에서 포도주를 제조하여 일반적인 제법으로 제조한 포도주와의 비교 분석 실험을 행하였다. 또한 GC-O(Gas chromatography-Olfactometry)와CC-MS(Gas chromatography-Mass spectrometry) 그리고, 훈련된 검사자들을 통한 관능검사의 실시로 그 차이점을 구명하였는데, 관능검사 결과 RO 28 시료가 General D사(社)의 제품에 비해 전체적인 선호도면에서 월등한 것으로 나타났다. GC-O및 GC-MS를 이용한 분석결과 RO 28시료가 General 및 D사(社) 제품에 비하여 훨씬 긍정적인 향기성분의 함량이 높은 것으로 나타났으며, 비교적 긍정적인 향기성분으로 사료되어지는 ethanol, ethyl acetate 등의 chemical compound를 동정해 낼 수 있었다.