• Title/Summary/Keyword: qualitative pollen analysis

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Reconstruction of Paleo-Temperature During the Holocene Using WA-PLS Analysis of Modern Pollen From the Surface Soil in the Southeastern Part of the Korean Peninsula (표층화분의 WA-PLS 분석을 통한 한반도 남동부지역 홀로세 고기온 복원)

  • Yoon, Soon-Ock;Hwang, Beomjin;Hwang, Sangill
    • Journal of The Geomorphological Association of Korea
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    • v.24 no.4
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    • pp.13-25
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    • 2017
  • To reconstruct the paleo-temperature quantitatively and to overcome limitation of traditional qualitative pollen analysis, this study was performed pollen analysis using the modern surface soil at Yulha-dong, Gimhae-si of southeastern part of Korean peninsula. Canonical Correspondence Analysis(CCA) was done to identify the most important environment variable about composition of modern surface pollen sample. Also, Weighted Average-Partial Least Square(WA-PLS) was analyzed to obtain modern surface pollen-temperature transfer function. The transfer function was applied to the results of qualitative fossil pollen analysis at Yulha-dong, Gimhae-si, Sampyung-ri, Ulsan-si and Taewha-dong, Ulsan-si. Therefore, the paleo-temperature was reconstructed during the Holocene since 6,200 yr BP. According to the results of the research, it is $1{\sim}2^{\circ}C$ lower than the current average annual temperature at the study area in 6,200 yr BP, since then it increased to the same level to the current temperature and decreased again until 2,600 yr BP. From the 2,600 yr BP, the temperature was fluctuated to the present.

Genetic Variation of a Single Pollen-derived Doubled Haploid Population in Rice

  • Moon, Huhn-Pal;Kang, Kyung-Ho;Ahn, Sang-Nag;Choi, Seon-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.4
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    • pp.250-253
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    • 1998
  • Somaclonal variation was observed in the field on doubled haploid plants derived from single pollen of a rice cultivar "Hwaseongbyeo". The variations of seven quantitative traits including plant height and one qualitative trait (pubescence) in 436 lines ($R_2$ generation) were analyzed. The number of lines which fell beyond the boundaries of the 95% confidence intervals of the check variety, Hwaseongbyeo was checked for each quantitative trait, and of those fertility showed the highest variation frequency (85.6%), followed by plant height (77.5%), flag leaf length (66.5%), grains per panicle (42.2%), days to heading (34.5%), panicle length (30.7%) and panicles per hill (22.7%). And the variations of quantitative traits except days to flowering appeared to move in the negative direction compared to "Hwaseongbyeo". Variability within lines was also observed for quantitative and qualitative traits. Twenty-nine $R_2$ lines (7%) segregated for pubescence and 130 $R_2$ lines (30%) showed variation with regard to fertility. This suggests that mutations usually occur before diploidization. Twenty-nine $R_2$ lines representing a wide spectrum of variation were chosen for RAPD analysis. The number of lines showing DNA polymorphism compared to Hwaseongbyeo ranged 0 from to 10 according to the primer used and this seems to indicate that specific loci have highly mutable genomic site.utable genomic site.

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A Systematic Study on Selected Amentiferous Plant Taxa - By Quantitative Analysis of Proteins - (유이화서 식물군의 통계분류학적 연구 - 단백질의 정량분석적 접근 -)

  • 이유성
    • Journal of Plant Biology
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    • v.28 no.3
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    • pp.207-216
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    • 1985
  • Radial immunodiffusion, rocket immunoelectrophoresis, and Boyden procedure have been employed as quantitative analysis of pollen proteins in a systematic investigation of selected amentiferous plant taxa. Data presented here are continued and supplementary to the previous qualitative analysis of immunoprecipitin systems for the same purpose. Although the number of taxa tested has been limited, the serological evidence indicates that the Betulaceae has the greatest similarity to the Fagaceae, next to the Juglandaceae, the least to the Salicaceae, when antisera against Alnus hirsuta and Betula platyphylla var. japonica were used for tests. Within the Betulaceae Alnus and Betula show greatly similar affinities together, but less similar to the rest of genera: Carpinus, Carya and Corylus. When antisera against Quercus aliena, Q. dentata, and Q. glauca were used for tests, the following decreasing order to serological affinities was obtained: Quercus Alnus, Betula Carpinus, Carya, Corylus Juglans, Pterocarya Populus. Overall serological data come closer to supportint the classification systems of Cronquist, Takhtajan, and Hutchinson; but less of Thorne and Bessey. In addition this investigation indicated that pollen, with its high protein content, provided an excellent source of extractable antigens for serosystematic researches.

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A Systematic Study on Selected Amentiferous Plant Taxa - By Qualitative Analysis of Proteins - (유이화서 식물군의 계통분류학적 연구 - 단백질의 정성분석적 접근 -)

  • 이유성
    • Journal of Plant Biology
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    • v.28 no.3
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    • pp.179-190
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    • 1985
  • A review of taxonomic treatments of the amentiferous plant taxa reveals diverse classification. It appears to be necessary to investigate new characteristics and attempt to determine the significance of these characteristics in terms of amentiferous systematics. Serological techniques have been empolyed in a systematic investigation of selected taxa of the Amentiferae: Betulaceae, Fagaceae, Juglandaceae, Myricaceae, and Salicaceae. Data by qualitative analysis of pollen proteins, double immunodiffusion and conventional immunoelectrophoresis, have proved to be valuable in delimitation of taxa tested. When the antisera against Alnus hirsuta, Betula platyphylla var. japonica, Quercus aliena, Q. dentata, Q. glauca were tested; Alnus and Betula have the greatest protein similarity to one another; and next to the rest of betulaceous genera. Relatively strong protein similarity obtained with most representatives of Quercus and Fagus when reacted with antisera against Alnus and Betula is very much in contrast to the weak protein similarity obtained for the genera of Juglandaceae, Myricaceae, particularly of Salicaceae Tested with the same antisera. When Quercus antisera were used for various genera, the weakest protein similarity was obtained with Populus.

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PALYNOLOGICAL ASSEMBLAGES FROM LATE CRETACEOUS TO TERTIARY DEPOSITS OF KACHI-I WELL, BLOCK II, YELLOW SEA BASIN, KOREA

  • YI Sangheon
    • 한국석유지질학회:학술대회논문집
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    • spring
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    • pp.1-11
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    • 1997
  • Thirty one samples from Late Cretaceous and Tertiary interval sections (468-783m) of the Kachi-I Well in Block II, Yellow Sea Basin, have been analysed for their terrestrially derived palynofloras. The systematic study of the palynomorphs recovered has yielded one hundred and fifty-five taxa; forty-three species of spores belonging to twenty-eight genera, seventy-seven pollen assignable to forty-three genera, and twenty-seven species assignable to fifteen genera and eight fungal remains. The results of both qualitative and quantitative analysis propose a succession of eight terrestrial palynomorph associations. Seven associations are erected in Late Maastrichtian and one in Early to Middle Miocene. Age determinations are on the basis of palynomorph taxa alone for the all associations. The Late Cretaceous/Tertiary unconformity is recognised at between 603 and 613m, based on the palynological data. The sedimentary basin during the Late Cretaceous seem to be lowland shallow marginal lacustrine with stagnant, mesotrophic conditions. On the other hand, the basin during the Early-Middle Miocene is considered to have been characterised by lowland swamp areas. The palaeoclimatic conditions during the Late Cretaceous are considered to be humid tropical to subtropical, while during the Early to Middle Miocene they are considered to be warm temperate with humid conditions. A comparison of palynomorph assemblages between the present study and the previous studies of Late Cretaceous in Circum-Pacific Northern Hemisphere is made, These assemblages reveal that lower sections (612-783m) of the Kachi-I well belong to the Late Cretaceous Aquilapollenites province of Herngreen and Chlonova (1981) and Srivastava (1981, 1994).

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Taxonomic Studies on Korean Oaks-Quercus- by Pollen Protein Analysis (한국산 참나무속 식물의 화분단백질분석에 의한 분류학적 연구)

  • 이유성;조무연
    • Journal of Plant Biology
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    • v.24 no.4
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    • pp.217-231
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    • 1981
  • This research was conducted to determine if the obtained serological data would reveal: (1) grouping among basic Quercus species, (2) the degree of correspondence within and among any detected grouping, and (3) the comparative value of new serological technique, "rocket immunoelectrophoresis". Overall quantitative and qualitative data indicate the genus Quercus is distantly separated from the Fagus. Within the Quercus, the subgenus Cyclobalanopsis is separable from the Lepidobalanus. But the elevation of the Cyclobalanopsis to the rank of a separate genus is not warranted. Antisera produced to the species belonging to Lepidobalanus gave strong reactions with other experimental species in the same subgenus. Particularly, Q. aliena, Q. donarium, and Q. serrata are tied up each other in terms of serological similarity. Rocket immunoelectrophoresis used in this systematic research proved to be a valuable new technique for systematic serology. To obtain the degree of protein similarity (serological correspondence) this technique was used in plant systematic research and determined by sum of total rocket heights. The rocket immunoelectrophoresis illustrated the measurement of individual immunoprecipitin systems identical or partly identical to those in the reference-reaction as quantitation of all available rocket heights.t heights.

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