• The Plant Pathology Journal
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• v.35 no.1
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• pp.1-10
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• 2019

Wheat blast occurred in Bangladesh for the first time in Asia in 2016. It is caused by a fungal pathogen, Magnaporthe oryzae Triticum (MoT) pathotype. In this review, we focused on the current status of the wheat blast in regard to host, pathogen, and environment. Despite the many efforts to control the disease, it expanded to neighboring regions including India, the world's second largest wheat producer. However, the disease occurrence has definitely decreased in quantity, because of many farmers chose to grow alternate crops according to the government's directions. Bangladesh government planned to introduce blast resistant cultivars but knowledges about genetics of resistance is limited. The genome analyses of the pathogen population revealed that the isolates caused wheat blast in Bangladesh are genetically close to a South American lineage of Magnaporthe oryzae. Understanding the genomes of virulent strains would be important to find target resistance genes for wheat breeding. Although the drier winter weather in Bangladesh was not favorable for development of wheat blast before, recent global warming and climate change are posing an increasing risk of disease development. Bangladesh outbreak in 2016 was likely to be facilitated by an extraordinary warm and humid weather in the affected districts before the harvest season. Coordinated international collaboration and steady financial supports are needed to mitigate the fearsome wheat blast in South Asia before it becomes a catastrophe.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.61-67
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• 1984

In an attempt to obtain a basic information to develop methods of an effective chemical control and disease forecasting of panicle blast of rice, effects of conidial number of the causal fungus, Pyricularia oryzae, collected in different periods and the rate of leaf blast occurrence on the occurrence of panicle blast were investigated. Conidial number the fungus collected in 5 days before and after heading date were closely related with panicle blast occurrence. But no relationship was obtained between the occurrence rate of leaf blast and that of panicle blast. Considering the incubation period of the disease, we presume that the most effective application periods of chemicals are 5-10 days and 10-15 days before heading, when immediate effective chemicals and slow effective chemicals are applied, respectively.

• Korean Journal of Agricultural Science
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• v.9 no.1
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• pp.275-283
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• 1982

One hundred and sixteen seed samples of 16 different crops were tested by routine seed health testing methods. Seeds of rice, soybean and barley were found to be associated with 18, 15 and 7 species of fungi, respectively. Discoloration and spotting of various kinds were observed in rice seeds. Epicoccum purpurascens, Pyricularia oryzae and Trichoconiella pedwickii were higher in these discoloured than in normal seeds of rice. Macrophomina phaseolina, the charcoal-rot fungus is a new record for soybean in Korea. Seeds of sesame were infected by 13 fungi Alternaria sesami, A. sesarmicola and Corynespora cassiicola were detected predominantly. Twenty one species of fungi were detected in seed samples of 12 commercial vegetable crops. Alternaria, Colletotrichum and Fusarium were found predominantly. Some seed samples of commercial vegetable crops were heavily infected with pathogenic fungi and showed heavy seed and seedling rot.

• Applied Biological Chemistry
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• v.35 no.1
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• pp.14-22
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• 1992

A series of new N-[1-(benzotriazol-1-yl)aryl]arylamine derivatives were synthesized and their antifungal activities $(pI_{50})$ in vitro against Pyricularia oryzae, Fusarium oxysporum f. sp. sesami, Valsa ceratosperma and Botrytis cinerea were dertermined by the agar medium dilution method. From the results of the quantitative structure-activity relationships $(QSAR_S)$ analysis, $hydrophobicity({\pi})$, $electronic({\Sigma\sigma})$ and molar $refractivity({\Sigma}M_R)$ parameter of X & Y-substituents on the phenyl group were also shown to be important factor in determining the variation in the antifungal activity. 4-Bromo group substituents (1d & 2b) were the most effective compounds and the $half-life(T_{1/2})$ on the hydrolysis of X(1) at netural pH was about 1.5 day. Molecular orbital(MO) functions of substrate compound, linear free energy relationships$(LFER_S)$ on the antifungal reactivity arid the results of molecular design were also discussed.

• The Korean Journal of Mycology
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• v.10 no.2
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• pp.67-73
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• 1982

The $(NH_4)_2\;SO_4$ (70%) treated crude enzymes from the culture filtrates of the$C-7^{+t}$ strain of Pyricularia oryzae which was grown on 2% CMC (carboxymethyl cellulose) for 8 days at $28^{\circ}C$ were chromatographied on Sephadex G-150 and DEAE-Sephadex A-25 columns. From the chromatography, three fractions of CMCase$(C_x)$ was examined using Na-CMC as substrate. The $C_x$ enzyme activity was optimal at pH 6.0 and $40^{\circ}C$, stable up to $40^{\circ}C$. The values of Km and Vmax of the enzyme were $2.8{\times}10\;mM$ and 5.9m moles/hour, respectively. The molecular weight determined by Sephadex G-150 column chromatography was around 80,000. Approximately sevenfold purified $C_x$ enzyme gave a single protein band on the polyacrylamide gel electrophoresis.

• Korean journal of applied entomology
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• v.13 no.4 s.21
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• pp.245-249
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• 1974

In order to establish a dose limits for subsequent induced mutation research itl Pyricularia oryzae, X-ray sensitivity of the conidia and the vegetative hypae of the fungus, race N-1, was investigated. Conidia of the fungus irradiated with X-rays reduced significantly in spore germination inversely with radiation doses. A severe suppression of conidia germination in about $80\%$ was found at the dose of 120kR, and the rests of the conidia produce very short and lysed germ tubes. A stimulated effect was observed in the elongation of hyphae from the conidia of 10 kR irradiation at initial stage of the growth. The radiosensitivity of hyphae was exremely higher than that of conidia with the increase of radiation doses. It was also recognized that the frequency of X-ray induced mutation in pathogenicity was directly proportional to radiation doses.

• Applied Biological Chemistry
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• v.33 no.3
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• pp.231-238
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• 1990

The structure-antifungal activity correlations between the structure of fourteen new 1-(phenoxymethyl)benzotriazoles (I) (Y=0), 1-(thiophenoxymethyl)benzotriazoles (ll) (Y=S) and 1-(azidomethyl)benzotriazole (III) derivatives were synthesized, and their activity, fifty percent inhibition of mycelial growth($pI_{50}$), in vitro against Pyricularia oryzae, Fusarium axysporum f.sp sesami, Valsa ceratosperma and Botrytis cinerea were investigated using a generalized QSAR method. The activity of (I) was superior In those of (II) and (III). The effect of the substituents (X) on the phenoxy group (I) was rationalized by a parabolic function of electronic (${\sigma}$), steric ($B_1$) and hydrophobic parameter(${\pi}$), and hydrogen bonding (HB). Where the optimal values of substituent on the fungicidal activity againt P. oryzae and F. axysporum f.sp.sesami are $B_1=1.40A;(H)$ and ${\pi}=0.07{\sim}0.15;(H)$, and those of substituent on the fungicidal activity against V. ceratosperma and B. cinerea are ${\sigma}=0.23{\sim}0.28;\;(C1),\;{\pi}=0.70;$ (C1), respectively. The most effective compound ( I a) and ( I d) were examined in this study.

• Korean journal of applied entomology
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• v.13 no.1 s.18
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• pp.19-23
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• 1974

Since Tongil has been cultivated widely in farmers' fields, the variety is known to be highly resistant to existing races of Pyricularia oryzae in Korea. However, Tongil and the sister lines were moderately susceptible to race IA-65, which was isolated from Tongil at the blast nursery of the College of Agriculture, Seoul National University, 1972. In field tests of 1973, pathogenic reactions of IR lines at the isolated nursery, where IA-65 had been inoculated, were significantly higher than those at the farm nursery. When seedlings of 196 IR lines were inoculated with IA­65, about $30\%$ of the IR lines including IR667-98 were moderately susceptible. Among the 30 isolates of P. oryzae obtained mostly from indicas, 12 were also differentiated in 1973 as IA group known to be the most prevalent races at IRRI in the Philippines, where Tongil has been highly susceptible. This IA group was also pathogenic to Tongil in greenhouse experiments. The race group causing moderately susceptible to Tongil should be new in Korea, since no occurrences of the race IA have ever been reported previously from the areas where japonica types of rice are cultivated. Because some races of P. oryzae present in Korea are pathogenic to Tongil and because these races may become prevalent in the fields, the reaction of Tongil must be continuously monitored.

• Applied Biological Chemistry
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• v.27 no.2
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• pp.95-99
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• 1984

Some derivatives of 3-oxo-1,2-benzisothiazole-1,1-dioxide were synthesized, and their antifungal activities against Pyricularia oryzae was determined by the agar medium dilution method. $I_{50}$ values of the candidate derivatives were shown to be as follows; 3-chloro(37.8ppm), 2-chloro(318.7ppm), MCS(20.1ppm), 2-allyl(946.2ppm), 3-(p-nitrophenyloxy) (35.4ppm), 3-(o-nitrophenyloxy) (11.8ppm), 3-(p-aminophenyloxy) (1643.2ppm), 2-chloromethyl(192.7ppm) and 2-hydroxymethyl derivative(248.4ppm).

• Microbiology and Biotechnology Letters
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• v.10 no.1
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• pp.39-43
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• 1982

t-Cinnamamide has been isolated from the culture filtrate of the isolates, Streptomyces. sp. No.297 The identity of the compound was established by UV and IR spectra, NMR, mass spectra and by chemical reactions. Through antimicrobial activity test using a two-fold serial agar dilution mothjod, t-cinnamamide showed strong growth inhibitory activity against Pellicularia sasakii, Pyriculario oryzae and some pathogenic fungi, but not inhibitory over procaryotes tested.