• 생명과학회지
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• 제16권3호
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• pp.387-395
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• 2006

유도된 집파리유통 hemolymph중에서 Candida albicans의 3가지 anti-fungal peptides를 분리하였다. 3개 anti-fungal peptides는 분자량이 4-16 kDa 사이의 분명한 구별이 있을 뿐만 아니라, 각 peptide는 anti-fungal peptides작용이 있었다. 이들 peptide의 공통 특징은 모두 열을 받은 뒤 활성이 변하지 않는 비교적 강한 내열성을 보여주었다.

• The Plant Pathology Journal
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• 제15권1호
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• pp.34-37
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• 1999

Cymbidium mosaic virus (CyMV) was purified from CyMV infected orchid plant leaves by Sephacryl S-500 HR column chromatography. Partial purification was done by solubilization with Triton X-100 (alkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG 6,000) followed by ultracentrifugation on 30% sucrose cushion. Based on the spectrophotometric analysis, 33 mg of CyMV could be obtained form 100 g of CyMV-infected orchid plant leaves. The purified CyMV represented one distinct homogeneous band by SDS-PAGE, and electron microscopy revealed that it was highly homogeneous and not fragmented. Bioassay demonstrated that the purified CyMV had a normal infectivity to Chenopodium amaranticolor and orchid plants. Based on these results, the purification method in this work could be served as an improved method for the purification of CyMV and similar viruses with good yield, high purity and native integrity.

• 한국해양공학회지
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• 제20권4호
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• pp.58-63
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• 2006

When the costal zone surrounded by a breakwater has a narrow vertical opening, currents in the vicinity of a narrow entrance can result in a jet flow, coinciding with the tide. Such a Tidal-Jet Generator(TJG) can change the water mass distribution and transport processes in the domain of influence of the jet. Also, it can decrease the residual time of them. In the present study, the water purification utilizing tidal jets in the coastal zone over constant bathymetry are estimated numerically, using a finite-difference numerical scheme, named the NS-MAC-TIDE method, which isbased on the fully 3D Navier-stokes(NS) equations. The shear velocity near the inlet of the TJG are predicted from the flow field simulation, and are assessed qualitatively with the development of scouring or sediment that is caused by the change of diffusion or sweeping flowup from the seabed of sediment particles. Finally, through solving a transport equation of concentration, the residual time related on mass transport processes and the flushing mechanism for water purification are investigated.

• 한국환경복원기술학회지
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• 제9권3호
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• pp.1-16
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• 2006

The purpose of this study is to develop design model of ecological park as stormwater storage facilities. The results are as follows : First, the design model of ecological park as stormwater storage facilities consider ecological and landscape characteristics such as high efficiency of land use, function as disaster prevention, ecological water purification, formation of habitat for flora and fauna. Second, this study demonstrates two types of plane structure and eight types of designed section. They can be combined and designed depending on conditions of each site. The facilities of stormwater storage conduct disaster prevention system and ecological park. Retention pond in stormwater storage facilities for ecological park also should be made for ecological restoration in the site. Third, the ecological park provide the basis for ecological network from in-site to out-site. Therefore its conservation and restoration plan consider the ecosystems of the site. Fourth, the most important factor for maintenance and management for retention pond is keeping water quality. Sustainable Structured wetland Biotop system is suggested for ecological water purification system in the retention pond which is one of the constructed wetland system using multi-celled aquatic plant and pond. This system can also provide habitat for animals and plants, water friendly park for men, and beautiful landscape.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1690-1698
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• 2014

Partial purification of glucoamylase from solid-state fermentation culture was, firstly, investigated by reverse micellar extraction (RME). To avoid back extraction problems, the glucoamylase was kept in the original aqueous phase, while the other undesired proteins/enzymes were moved to the reverse micellar organic phase. The individual and interaction effects of main factors (i.e., pH and NaCl concentration in the aqueous phase, and concentration of sodium bis-2-ethyl-hexyl-sulfosuccinate (AOT) in the organic phase) were studied using response surface methodology. The optimum conditions for the maximum recovery of the enzyme were pH 2.75, 100 mM NaCl, and 200 mM AOT. Furthermore, the optimum organic to aqueous volume ratio ($V_{org}/V_{aq}$) and appropriate number of sequential extraction stages were 2 and 3, respectively. Finally, 60% of the undesired enzymes including proteases and xylanases were removed from the aqueous phase, while 140% of glucoamylase activity was recovered in the aqueous phase and the purification factor of glucoamylase was found to be 3.0-fold.

• 청정기술
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• 제19권3호
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• pp.342-346
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• 2013

본 연구에서는 분무열분해(spray pyrolysis)에 의해 다양한 혼합비의 다공성 메조기공 실리카-알루미나 흡착제를 제조하여 항암물질 파클리탁셀(paclitaxel)의 정제에 이용하였다. 흡착제 처리와 헥산 침전을 수행한 결과, $S_{0.5}A_{0.5}$ (Si/Al 몰비 = 0.5/0.5)을 사용하였을 때 가장 높은 순도 (~27%)와 수율 (~63%)의 파클리탁셀을 얻을 수 있었다. 이러한 불순물(타르 및 왁스 성분 포함) 제거 효과는 흡착제에 붙은 유기물의 TGA 정량 분석 결과로도 확인할 수 있었다.

• 한국응용과학기술학회지
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• 제24권4호
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• pp.383-392
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• 2007

This paper is studied on the efficient purification process of dimethyl carbonate (DMC) from the melt layer crystallization combining crystallization process, sweating process and distillation recovery process. Purity and yield of DMC crystal depended mainly on the crystallization temperature, cooling rate, sweating termperature sweating rate. Through the optimization of crystallization and sweating operation, DMC crystal can be upgraded to very high purity over 99.9% and high yield over 85%.

• 설비공학논문집
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• 제28권12호
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• pp.509-514
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• 2016

From the point of view to prevent airborne infection-related diseases such as H1N1, SARS, and MERS, an actual application of air cleaning and purification systems including technologies like UVGI has become increasingly important. Recently, an air purification system using REME (Reflective Electro Magnetic Energy) developed in the U.S. is applied for indoor air purification and sterilization technology to counteract the outbreak of new airborne infections. In this study, an air sterilization performance experiment using REME was carried out. The results verified that air sterilization performance in the case of installing a REME system in a medical center was 31%, namely the number of floating bacteria decreased by 31% after only a five-day operation. In addition, the number of culture collections in the REME operating air conditioning systems using nonpathogenic Geobacillus stearothemophilus as a biological indicator decreased maximally to 67%. A field application of REME technology will be useful to prevent airborne infection-related diseases, especially in response to public health crises due to the advent of emerging diseases.

• 상하수도학회지
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• 제33권4호
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• pp.311-319
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• 2019

The purpose of this study was to analyze water treatment characteristics, including the efficiency of removing algae from water purification plants, by installing a demonstration facility for decontamination of algae, including natural algae remover injection equipment, in the water purification plant. Jar-test showed that the optimum injection of natural decontaminant was 20 mg/L. Of the water contaminant treatment efficiency of the intake and water purification plants, Chl-a averaged 74.0% elimination efficiency from $5.0mg/m^3$ to $1.3mg/m^3$ and the maximum treatment efficiency was 91.5% removal efficiency when the inflow concentration of Chl-a was $11.8mg/m^3$. In addition, 51.2% and 47.1% of the taste and odor indicator items, geosmin and 2-MIB, resulted from the overgrowth and decaying of algae, respectively, to identify toxic substances and odor reduction effects. In addition, elimination efficiencies of SS and Turbidity materials were higher than 70.0%. In the injection of natural algae remover, no effects such as sudden changes in water quality due to secondary reactions were found, and appropriate levels were maintained under water treatment conditions.

• 공업화학
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• 제32권3호
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• pp.290-298
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• 2021

천연 흑연의 음극재 적용을 위하여 정제 공정을 실시하였으며, 공정에 따른 흑연의 구조적 변화와 불순물 함량이 음극 특성에 미치는 영향을 고찰하였다. 천연 흑연은 불화암모늄과 황산을 동일 비로 하여 사용량을 달리한 산처리 및 온도(800~2500 ℃)를 달리한 열처리를 통하여 화학적/물리적으로 정제되었다. 산을 이용한 불순물 제거는 한계가 있었으며, 이후 진행된 2500 ℃까지의 열처리를 통해 Si과 같은 일부 원소를 제외하고 대부분의 불순물이 전량 제거되는 것을 확인하였다. 복합 정제 공정에 따라 제조된 흑연 음극재의 특성이 향상되었으며, 구조와 불순물 함량 변화는 각각 용량 및 속도 특성과 초기 쿨롱 효율에 지배적인 영향을 미쳤다. 복합 정제 공정은 흑연 구조를 향상시켰으며, 불순물을 효율적으로 제거하여 SEI층 형성 억제 및 Li⁺ 삽입 공간 확대를 통해 리튬 이온 전지의 성능을 향상시켰다.