• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.27-31
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• 2002

Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The $paclitaxel-Cenexol^{TH}-is$ commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.

• Journal of Plant Biotechnology
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• 제29권1호
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• pp.59-62
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• 2002

Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The paclitaxel-Genexol$^{TM}$-is commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.

• 한국환경과학회지
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• 제21권7호
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• pp.825-835
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• 2012

This study for the development of water purification Artificial floating island maximizing domestic Artificial floating island patent trends and product development, according to the timing of patent registration was analyzed for trends. In addition, domestic invention patent technology Artificial floating island typed according to the purpose and characteristics of domestic patents were Artificial Floating Island. In particular, domestic leisure space with a growing population and the need for securing emerging role as a reservoir of water only in the past, who do appeal as a tourist destination or as an ecological space utilized, and accordingly will transform and the need to secure a hydrophilic, degrade water quality problems using this aquatic environment (water acquisition and hydrophilic), the requirements are a big obstacle is the reality factor. This patented product differentiation strategy through the analysis of the development of technology progressiveness (Field Application) in terms of water quality improvement and maintenance side, and the hydrophilic side scenery, ecological restoration aspects, and applicability to the field and taking into account existing technology economic aspects of distinction were presented and advertised a lot in terms of cost compared to other techniques without the use of highly efficient methodology for building a water purification and also appears identity appeal, wetlands, rivers, etc. can be applied broadly technician widespread deployment and installation time to less simple and more are expected to spread.

• 한국응용과학기술학회지
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• 제24권2호
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• pp.140-148
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• 2007

As an additional high purification method of p-dioxanone monomer for a high molecular weight polymer, the sweating operation of crystalline layer obtained by layered melt crystallization from p-dioxanone-diethylene glycol system was studied. Purity and yield of p-dioxanone crystal depended mainly on the sweating temperature and sweating time. Increasing sweating time and sweating temperature, the purity of p-dioxanone crystal increase, whereas the yield of that decrease, respectively. Through the optimization of sweating operation, p-dioxanone crystal can be upgraded to very high purity over 99.9 % suited to monomer for polymerization.

• 한국가스학회:학술대회논문집
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• 한국가스학회 2005년도 추계학술발표회 논문집
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• pp.141-145
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• 2005

DME (Di-Methyl Ether) is a new clean fuel and an environmental-friendly energy resource, also is recently increasing with an alternative interest because of the industrial use. DME has been shown to have excellent properties as a diesel fuel giving emission level better than ULEV standard. So it has been attracting considerable as an alternative diesel fuel. In this study, we carried out simulation of separation and purification process of demo plant for 101on per day DME production, which cause the effect that is important in productivity, from operation results of pilot plant for 50kg per day DME production. The liquefied stream, which was separated by gas-liquid separator after DME reactor, includes $CO_2$, DME, Methanol and $H_2O$. We established three distillation columns for separation and purification of the stream. $CO_2$ was extracted from the stream by first distillation column, DME was extracted by second column and Methanol was extracted by third column. We investigated and analyzed the effect which the actual operation variables cause in efficiency of process and optimized process, finally we got the DME of purity $100\%$.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1369-1379
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• 2010

This study investigated purification and milk-clotting activity of the enzymes produced by Bacillus subtilis var, natto and Rhizopus oligosporus compared with that of commercial rennet. The clotting time, viscosity, tension and microstructure of the curd and electrophoretic patterns of milk proteins were determined. The milk-clotting activity/proteolytic activity ratios (MCA/PA ratio) of B. subtilis, R. oligosporus and commercial rennet were also compared. The results revealed that the curd formed by the commercial rennet had the highest viscosity and curd tension and the shortest clotting time among the three enzymes. However, curd produced by Rhizopus enzymes was ranked as second. From the MCA/PA ratio and electrophoretogram analyses it could be concluded that the enzyme produced by B. subtilis had the highest proteolytic activity, while the commercial rennet had the highest milk-clotting activity. Observations of microstructures of SEM showed that the three-dimensional network for curd formed by commercial rennet was denser, firmer and more smooth. The milk-clotting activity, specific activity, purification ratio and recovery of the purified enzymes produced by both the tested organisms were also determined with ion exchange chromatography and gel filtration.

• 한국식품영양과학회지
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• 제18권3호
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• pp.279-286
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• 1989

Alkaline protease 생성능이 강한 Aspergillus fumigatus을 토양에서 분리동정하고, 효소생산조건을 구명한 결과 $30^{\circ}C$에서 3일간 배양하였을 때 최고활성을 나타냈으며 생산된 조효소를 황산암모늄염석, Sephadex G-25, G-150 gel filteration과 DEAE-Cellulose컬럼 chromatography로 정제하여 수율 6.4%, 정제정도 86.13배의 효소를 얻었고 polyacrylamide gel 전기영동에 의해 단일밴드인 것을 확인하였다. 정제효소의 분자량은 63000정도 였으며, 17종의 아미노산으로 구성되어 있고, 그 중 glycine과 glutamic acid가 가장 많고 methionine과 cystine이 가장 적었다.

• Korean Chemical Engineering Research
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• 제57권4호
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• pp.469-474
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• 2019

Fermentation of palm oil mill effluent (POME) produces biohydrogen in a mixture at a specific set condition. This research was conducted to purify the produced mixed biohydrogen via absorption and membrane techniques. Three different solvents, methyl ethanolamine (MEA), ammonia ($NH_3$) and potassium hydroxide (KOH) solutions, were used in absorption technique. The highest $H_2$ purity was found using 1M MEA solution with 5.0 ml/s feed mixed gas flow rate at 60 minutes absorption time. Meanwhile, the purified biohydrogen using a polysulfone membrane had the highest $H_2$ purity at 2~3 bar operating pressure. Upon testing with proton exchange membrane fuel cell (PEMFC), the highest current and power produced at 100% $H_2$ were 1.66 A and 8.1 W, while the lowest were produced at 50/50 vol% $H_2/CO_2$ (0.32 A and 0.49 W). These results proved that both purification techniques have significant potential for $H_2$ purification efficiency.

• 상하수도학회지
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• 제32권6호
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• pp.487-497
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• 2018

Water quality improvement processes for stagnant area consist mainly of technologies applying vegetation and artificial water circulation, and these existing technologies have some limits to handle pollution loads effectively. To improve the purification efficiency, eco-friendly technologies should be developed that can reinforce self-purification functions. In this study, a multi-functional floating island combined with physical chemical biological functions ((1) flotation and oxidization using microbubbles, (2) vegetation purification and (3) bio-filtration with improved adsorption capacity) has been developed and basic experiments were performed to determine the optimal combination conditions for each unit process. It has been shown that it is desirable to operate the microbubble unit process under conditions greater than $3.5kgf/cm^2$. In vegetation purification unit process, Yellow Iris (Iris pseudacorus) was suggested to be suitable considering water quality, landscape improvement and maintenance. When granular red-mud was applied to the bio-filtration unit process, it was found that T-P removal efficiency was good and its value was also stable for various linear velocity conditions. The appropriate thickness of filter media was suggested between 30 and 45 cm. In this study, the optimal design and operating parameters of the multi-functional floating island have been presented based on the results of the basic experiments of each unit process.

• 한국가스학회지
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• 제10권2호
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• pp.22-26
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• 2006

본 연구에서는 하루 50 kg의 DME를 생산하기 위한 파일럿 플랜트를 Aspen Plus를 사용하여 전산모사 하였다. 전체 DME 분리공정의 모델링을 위하여 NRTL 액체 활동도계수 모델식을 사용하였으며 light 가스 성분의 용매의 용해도 추산을 위해서 Henry의 법칙을 사용하였으며 기상의 비이상성을 계산하기 위해서 SRK 상태방정식 모델식을 사용하였다. 전산모사 결과로 DME 분리탑의 중간 제품으로써 98 wt% 이상의 순도를 갖는 DME를 얻을 수 있었다.