• The Korean Journal of Pesticide Science
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• v.14 no.4
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• pp.394-400
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• 2010

Insecticidal activities of methanol extracts of the different parts of Prunus mume were investigated against the larvae of Cnaphalocrocis medinalis (C. medinalis) by topical application. The mortalities of the larvae of C. medinalis were 34% at the concentration of 4,000 ppm, 36% of 8,000 ppm, 40% of 16,000 ppm and 58% of 32,000 ppm of the extracts from the fruits, and 38% of 4,000 ppm, 45% of 8,000 ppm, 58% of 16,000 ppm and 75% of 32,000 ppm from the stem + flower of Prunus mume (P. mume), respectively. The rates of pupation of C. medinalis treated with either fruit or stem+flower were gradually low according to the concentration of extracts increased. The weight of pupae of C. medinalis has no significantly different in between the control and the treated groups of the extracts from both of fruit and stem+flower. However, the differences of the pupal weight and the rate of pupation of C. medinalis treated with the extracts from the stem+flower and fruit of P. mume were significant as the level of 56% ($R^2$=0.56363, P=0.4364). Both of stem+flower and fruit extracts of P. mume showed good effects of anti-feeding against C. medinalis. The extracts of the stem+flower of P. mume could be advocated for developing as birational agent for the control of C. medinalis.

• Journal of Sericultural and Entomological Science
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• v.46 no.2
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• pp.58-64
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• 2004

The current study was performed to clarify the pupation disorder symptoms in silkworm which herbicide, molinate has been regarded as a causal origin. Molinate residues in mulberry leaves was monitored in major rearing area of silkworm; Yeacheon, Sangju and Namwon. Detected range of the molinate in mulberry leaves was 0.002 to 0.013 mg/kg and detection frequency was 30.0 to 81.8%. Molinate concentration in the air of mulberry tree growing area was $0.004\;{\mu}g/m^3$. Water diluted solution of molinate at the concentration of 1, 10, 100 mg/l gave no abnormal symptoms in silkworm irrespective of spraying to silkworm or spiking to diet. Exposing the silkworm to the air containing 250, 2,500, and $10,000\;{\mu}g/m^3$ also revealed no abnormal symptom. These results illustrated that the abnormal growth of silkworm encountered in the field was not caused by pesticide contamination.

• Korean journal of applied entomology
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• v.43 no.3 s.136
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• pp.201-209
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• 2004

Overwintering ecology of the peach pyralid moth, Dichocrosis punctiferalis Guenee (Lepidoptera: Pyralidae) was studied in laboratory and in a chestnut orchard in 2001/2002 winter and 2003 spring. All twenty-four female and fourteen male moths were fruit-feeding type which were emerged from the larvae collected at the same chestnut orchard in 2003 winter. Periodical thermal treatment of overwintering larvae at $25^{\circ}C$ revealed that diapause termination of the larval population started from the end of January and was completed until April 20 in southern region of Korea. Pupation of diapause-terminated larvae started from early May and reached to $100\%$ at June 3 in chestnut orchard. Days to pupation of female larvae after diapause termination were 12.5, 8.9, and 7.5 at $20^{\circ}C,\;25^{\circ}C$, and $30^{\circ}C$, respectively. Those of male larvae were similar to those of females. Pupal periods of female were 14.7, 11.8, and 9.0 days at the three temperatures. Moths of the overwintered generation emerged from May 20 to June 28, and reached to $50\%$ emergence on June 8 to 9 under emergence cage. Attraction of the male moths of overwintered generation to sex pheromone traps showed that the date of $50\%$ catches to the traps were June 17, when data from 3 southern regions of Korea were pooled.

• Korean journal of applied entomology
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• v.53 no.4
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• pp.367-373
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• 2014

In order to investigate the impact of recycling agricultural wastes as feed for mealworm (Tenebrio molitor), we evaluated the replacing effect by the different level of tangerine shell, Chinese cabbage, king oyster mushroom (Pleurotus eryngii) and Enoki mushroom (Flammulina velutipes) substrates on wheat bran feed. Larval survival rate, larval weight, developmental period of larva, pupation rate and pupal weight were evaluated. In tangerine shell and Chinese cabbage replacement group, no replacing effects found. In all groups replacing by spent King oyster mushroom (Pleurotus eryngii) substrate, survival rate of larva was similar to that of control group but larvae weighed less than control group significantly. Developmental period of larva increases in the group of King oyster mushroom substrate replacement. Larval and pupal weight in Enoki mushroom (Flammulina velutipes) substrate by the level of 40 and 50%, replacement effect showed best results in successive breeding considering pupation rate. It is concluded that replacing 40 and 50% of Enoki mushroom (Flammulina velutipes) substrate is appropriate to substituted diet of Tenebrio molitor larvae.

• Korean journal of applied entomology
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• v.52 no.3
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• pp.227-237
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• 2013

The black soldier fly (BSF), Hermetia illucens, is a generalist detritivore that is usually exploited for recycling environmental wastes, including food remnants. These insects can also be used to produce antibiotics for medical applications. For this purpose, flies need to be mass-reared using an automatic rearing system that generates various sounds. Insects use sound as a means of communication for mating or warning against predators. Thus, extrinsic sounds may interrupt the normal physiological processes of insects. In this study, we analyzed the physiological changes due to various sound treatments at $25^{\circ}C$ and 8:16 h (L:D) photoperiod. At 95 dB, larvae were exposed to different sound frequencies (0-5,000 Hz). The treated larvae were adversely affected by the high-frequency sounds and showed significant reduction in pupation and adult emergence. Different sound intensities (0-105 dB) at 5,000 Hz caused significant changes in the pupation and survival of the adults at 85 dB or above. Under a stress sound condition (5,000 Hz, 95 dB), the BSF larvae exhibited aberrations in the expression of genes related to the immune system and digestion. These results indicate that high-frequency sound treatments at or above 85 dB act as a stress sound to BSF development.

• Journal of Sericultural and Entomological Science
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• v.29 no.1
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• pp.20-30
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• 1987

In the hemolymph proteins of the silkworm reared with mulberry leaves and artificial diet, electrophoretic patterns of Storege Protein 1 (SP1) and Vitellogenin(VG), changes of relative concentration of SP1, JHA effects during the developmental stages, and estimation of the molecular weight of vitellin subunits were investigated in the present study. 1. Storage Protein 1 (SP1) showed female specificity from the middle stage of the fifth instar to the end of spinning stage in the silkworm reared with mulberry leaves and artificial diet. 2. Vitellogenin (VG) showed female specififcity just afrter pupation in the silkworm reared with mulberry leaves and artificial diet. 3. Storage Protein 2 (SP2) without female specificity was detected from the early stage of the fifth instar to the first or second day after pupation. 4. Vitellin was composed of two non-identical subunits (vitellin-heavy chain ; VTL-H, vitellinlight chain ; VTL-L) with mplecular weight of 186,000 and 41,000. Also, there were no differenes between the molecular weights of vitellin subunits obtained from silkworms reared with mulberry leaves and artificial diet. 5. Relative concentration of Storage Protein 1 (SP1) after topical application - 0.5, and 10$\mu\textrm{g}$ per body weight - JHA on the 60th hour of the fifth instar showed the highest increase with the treatment of 5$\mu\textrm{g}$ and a higher increase with the treatment of 10$\mu\textrm{g}$ compared with the control (no topical application of JHA).

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.1
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• pp.1-4
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• 2011

This study was conducted to test the use of colored cocoon silkworm, Golden Silk, as a host for the fruiting body production of Cordyceps mushroom. Golden Silk variety had a shorter larval period than Yangwonjam at $25.3^{\circ}C$ and 78.5% RH: The average larval period of the Golden Silk was 24 days and 7 hr, and that of the Yangwonjam was 24 days and 15 hr. In the infection test of I. tenuipes on silkworm, the larval period of normal silkworms was longer than the silkworms inoculated with I. tenuipes in both Golden Silk and Yangwonjam. The pupae survival percentage of Golden Silk was 94.4%, whereas it was 91.9% in Yangwonjam. Golden Silk had a higher pupation rate than Yangwonjam. The pupation rate of normal silkworms was about 5% higher than that of silkworms inoculated with I. tenuipes. Infection rate of I. tenuipes into larvae of 5th instar newly exuviated silkworm was 93.8% in Golden Silk, and 96.9% in Yangwonjam. Synnemata production of I. tenuipes was better in Yangwonjam with an incidence rate of 99.8% than Golden Silk with 98.6%. The average synnemata weight of Golden Silk was 1.12 g and that of Yangwonjam 1.29 g. Golden Silk had a lower synnemata weight than Yangwonjam. But no significant difference was observed in synnemata weight varieties. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and similar in shape and color to wild synnemata collected in Korea.

• Korean journal of applied entomology
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• v.40 no.4
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• pp.297-300
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• 2001

Development and reproduction of the persimmon fruit moth, Stathmopoda masinissa, were investigated under different temperatures (15, 20, 25, and 3$0^{\circ}C$). It took 96.1 days to grow from egg to adult at 2$0^{\circ}C$, 43.2 days at $25^{\circ}C$, and 34.6 days at 3$0^{\circ}C$. At 15$^{\circ}C$, all tested individuals died before pupation. The developmental threshold temperatures for egg, larva, pupa, and adult were 12.2, 13.5, 13.8, and 13.4$^{\circ}C$, respectively. The total effective temperatures for egg, larva, pupa, and egg to adult were 74.0, 331.3, 160.5, and 569.9 degree days, respectively. The hatching, pupation, and emergence rates were highest at $25^{\circ}C$. The average life span of adult prior to laying eggs and the total life span of adult were 12.6 and 29.3 days at 2$0^{\circ}C$, 3.8 and 8.6 days at $25^{\circ}C$, and 2.5 and 7.0 days at 3$0^{\circ}C$, respectively. Mean generation time in days (T) was shorter at higher temperature. Net reproductive rate per generation (R$_{o}$) was lowest at 2$0^{\circ}C$. The intrinsic rate of natural increase (r$_{m}$) was highest at $25^{\circ}C$ as 0.066.

• Korean journal of applied entomology
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• v.25 no.1 s.66
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• pp.1-9
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• 1986

Periodical samplings were made in the fields, located Hwasong-Gun, Banwal-Myon, Doondai-Ri, for two years to study the major factors responsible to the population of the overwintered pine needle gall midge, Thecodiplosis japonensis Uchida et Inouye. The population densities of the overwintered larvae reduced greatly at the time of pupation and the changing patterns seemed to be influenced by environmental conditions. The estimated 50% pupation day was about two weeks earlier for the 1980 generation compared with the 1979 generation, and much higher average and the daily maximum temperatures in 1981 affected on the development of the larvae. The relative emergence rates were 14.1% for 1979 and 14.9% for 1980 generation. The relative emergence rates(Y) were affected by the moisture contents of soil ($X_1$) and its variance $(X_2),\;Y=-68.41+4.3206X_1-0.6887X_2$. The relative emergence rates seemed to be decreased with the increased variance of the moisture contents of soil. Percents of needle gall were 49.63% for 1980 and 86.87% for 1981 generation.

• Korean journal of applied entomology
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• v.44 no.2
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• pp.125-130
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• 2005

The ecological characteristics of the specialist insect predator, Oligota kashmirica benefica, were investigated for developing artificial rearing method with special interest on the oviposition behavior, the pattern of adult emergence and temperature in storage, and its effect on the density of the citrus red mite, Panonychus citri. Female beetle oviposited eggs mainly, $95\%$ of the eggs, on the bottom of leaves of the yuzu tree, and $91.3\%$ of them were covered with ecdysis skin of the red mites or the feces of themselves. The rate of adult emergence of the beetle rose up to $86.7\%$ when the horticultural media was provided for pupation from $60\%$ when it pupated in upland soil. Most larvae, $88\%$ of them, were found from the surface to the depth of 2 cm in the horticultural media. The optimum temperature for the storage of the adult beetle was found to be $12^{\circ}C$, at this temperature the 96.7, 73.3 and $70.0\%$ of the beetle survived for 10, 20, and 30 days, respectively. The control effect of the citrus red mite was appeared highest by releasing the beetle at the rate of one beetle against 150 mites.