• International Journal of Industrial Entomology and Biomaterials
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• 제35권2호
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• pp.106-110
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• 2017

The developmental responses of insects to temperature are important considerations in gaining a better understanding of their ecology and life histories. Temperature dependent models permit examination of the effect of temperature on the geographical distributions, population dynamics, and management of insects. The measurements of insect developmental and survival responses to temperature pose practical challenges that depend. The developmental characteristics of A. diaperinus were investigated at four temperature regimes (20, 25, 30 and $35^{\circ}C$), a relative humidity of 60%, and a light:dark photoperiod of 16:8h. The developmental time from larva to adult was 129.0, 49.8, 40.5 and 31.9 days at temperatures of 20, 25, 30 and $35^{\circ}C$, respectively. Pupal rate was 80.0%, 100%, 83.3% and 91.7% at temperatures of 20, 25, 30 and 35 respectively. There is an increasing need for a standardized manual for rearing this. Pupa had significantly lower weights at $35^{\circ}C$ than at the other temperatures. Female pupae (20mg) were significantly heavier than male (17mg).

• International Journal of Industrial Entomology and Biomaterials
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• 제38권2호
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• pp.31-37
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• 2019

Silkworm larval mortality specifically during spinning stage leading to non-spinning with specific morphological symptoms was frequently complained by sericulturists in Karnataka, India during 2015. Survey was conducted and information collected through questionnaire from the identified farmers who faced the problem of non-spinning in both traditional and non-traditional areas of Karnataka. Survey results indicate that the problem is specific to the silkworm crop of those farmers' who shifted from other crops of agriculture/ horticulture/olericulture/ floriculture to Sericulture. Silkworm rearing performance of the batches fed with these leaves confirmed that the pesticide sprayed to the crops previous to mulberry, remain in the soil and when mulberry plantation is taken up in these gardens, the pesticide is absorbed by the roots of mulberry plants and transported to the leaves. Silkworms that feed on these mulberry leaves, grow and ripen normally but during spinning stage, larvae die with external symptoms like regurgitation, body shrinkage, rectal protrusion later become hook shaped leading to non-spinning or partial metamorphosis into pupa and death or spin flimsy cocoons. Larval mortality ranged from a minimum of twenty five percent to a maximum of hundred percent. The problem was noticed from the first harvest of leaves and lasted for a maximum period of 36 months. Cocoon crop loss depends on the concentration, duration and type of pesticides used previously for other crops.

• 식품과학과 산업
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• 제49권4호
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• pp.31-39
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• 2016

The reasons for selecting insects as future food are high growth and feed conversion rates, reproduce quickly, low environmental footprint, valuable source of nutrients, and source of a various undeveloped functional materials. Since 2014, Tenebrio molitor larva, Protaetia brevitarsis larva, Allomyrina dichotoma larva, and Gryllus bimaculatus were registered as new food in Korea because it has been scientifically proven that they are not harmful to eat and nutritious. Therefore they can be legally produced and sold as food. Accordingly, there are 7 species of edible insects including grasshopper, silkworm pupa, and Baekgangjam in Korea. To improve aversion to edible insects, using their powder, chop, and gravy hidden their morphology, we developed more than 100 different kinds of recipes for general food menu, held several times tasting events to be familiar with edible insect food, and published cookbooks. Moreover, we developed more than 50 kinds of recipes for patients. To eat the insect food more and more people, we have been analyzed various function of insects. If health food based on the results of functional analysis is developed, edible insect's value will be raised. If various insect foods are developed and consumed by people, edible insect market will grow up more than 100 billion won in 2020.

• Journal of Species Research
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• 제11권2호
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• pp.117-127
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• 2022

This study is based on crane fly specimens collected during an 80-year period (1937-2017) in the Republic of Korea and Democratic People's Republic of Korea, and are in collections maintained at the United States National Museum, Smithsonian Institution, Washington DC, USA; the Snow Entomological Museum, University of Kansas, Lawrence, KS, USA; and the National Institute of Biological Resources, Incheon, South Korea. These crane flies are developing in wet places which usually prevail at lower altitudes, but such areas are often used for agriculture and human settlements, making natural habitats scarce and fragmented. Probably because of that, Limnophila crane flies are rare on the Korean Peninsula and were unnoticed by previous researchers. The genus Limnophila Macquart, 1834 with three species being new record for the Korean Peninsula: L. (Limnophila) japonica Alexander, 1913, L. (L.) pictipennis (Meigen, 1818) and L. (L.) politostriata Alexander, 1934a. General information are presented for each species, such as on genus and subgenus, redescriptions of species based on Korean specimens, illustrations of both sexes, elevation range, period of activity, habitat information, general distribution, and a distribution map for the Korean Peninsula.

• Journal of Species Research
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• 제11권1호
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• pp.38-46
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• 2022

This study is based on crane fly specimens collected during more than 80 years, starting from 1938 through 2019, in the Republic of Korea and Democratic People's Republic of Korea and are in collections maintained at the United States National Museum, Smithsonian Institution, Washington DC, USA; the Snow Entomological Museum, University of Kansas, Lawrence, KS, USA; and the National Institute of Biological Resources, Incheon, South Korea. Pilaria crane flies are aquatic and semiaquatic, developing in fast running and stagnant water bodies and in wet muddy places at the margins of water pools. Such habitats usually prevail at lower altitudes, but these areas most often are used for agriculture and human settlement, making natural habitats scarce and fragmented. Pilaria crane flies are rare, thus it is not surprising that genus was left unnoticed by previous researchers. The genus Pilaria Sintenis, 1889 with two species P. melanota Alexander, 1922 and P. simulans Savchenko, 1983, is a new record for the Korean Peninsula. We present general information on the genus, redescriptions of species based on Korean specimens, illustrations of both sexes, elevation range, period of activity, habitat information, general distribution, and a distribution map for the Korean Peninsula for each species.

• 한국잠사곤충학회지
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• 제7권
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• pp.49-52
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• 1967

가잠의 원종 및 교잡종에 있어서 감잠비율의 증감에 따른 자웅잠 생존율의 성차를 알고저 66년 하기와 추기사육에서 얻은 47, 68구를 원료로 하여 분석한 결과는 다음과 같다. 1. 원종중 일본종인 모단과 동교잡종모단$\times$대동에서는 감잠비율의 증감이 자웅차에 아무런 영향이 없었으므로 종전처럼 웅잠이 반듯이 자잠보다 강하다고는 할 수 없다. 2. 원종중 중국종, 대동에 있어서는 감잠비율이 많은 구일수록 웅용의 생존비율이 증가되며 그 정도는 y=0.233x＋41.67(y : 웅용생존율)이었다. 3. 이상의 결과로서 적어도 중국종 견질조사상 자웅견동수를 채취 조사하는 현행방법은 잠작에 따라 보정하여 줘야만 모집단의 치에 접근된다고 있다.

• Journal of Nutrition and Health
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• 제37권10호
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• pp.888-898
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• 2004

This study was designed to investigate the effects of 8-week resistance exercise by using dumbell on the body com-position, serum hormone profiles and feeding patterns in young and healthy female students with over 30% of body fat, aged 22 years in Masan, Korea. The subjects were all six, performed successfully the entire exercise-program. Anthropometry, serum lipid and neurohormone profiles of subjects were analyzed at 0, 4 and 8 weeks after exercise. And also their food and nutrient intakes were self-reported by 3-day record and food frequency questionnaire. Triceps skinfold-thickness of the subject students significantly reduced after the exercise to 46% less than before (p < 0.002) . The serum concentrations of leptin, insulin and catecholamine appeared to decrease steadily, but not significantly. Total protein in serum increased significantly (p<0.05), and Na (p<0.005), K (p<0.001) and Fe (p<0.058) concentrations increased remarkably within the normal range. The 8-week resistance exercise tended to induce fiber consumption (p<0.089) of subject students and changed the P/M/S ratio changed into the desirable way from 0.8 : 1 : 1 to 1 : 1 : 1, and that of n-6/n-3 PUPA from 11 : 1 to 6 : 1. After taking exercise, the intake frequency of cookies (p<0.058) and raymeun (p<0.085) decreased, but finally this feeding pattern returned to the way as before even though at marginal significance. Consequently the resistance exercise may induce the desirable changes of body fat and improve serum lipid profiles and feeding patterns in young obese females.

• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.16-20
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• 2000

The aqueous extract from the cultural mycelium of Paecilomyces japonica showed cytotoxicity against several tumor cells including Jurkat, U937, HL-60, HepG2, BW5147.G.1.4, and NIH3T3. When the aqueous extract was fractionated by sequential organic solvent extractions using n-hexane and ethyl acetate, the ethyl acetate fraction appeared to contain the most cytotoxic activity, and the $IC_{50}$ values for various tumor cells were in the range from 1.5 to $10.0{\;}\mu\textrm{g}/ml$. To elucidate the cellular mechanism underlying the induced cytotoxicity, the apoptotic DNA fragmentation along with the cell cycle proression was examined in Jurkat T cells following the ethyl acetate fraction treatment. In the presence of $2.5{\;}\mu\textrm{g}/ml$ of the ethyl acetate fraction, apoptotic DNA fragmentation of the cells was detected within 1 h and increased upto 24 h in a time-dependent manner. Under the same conditions, a sub-G1 peak was detectable by flow cytometry. These results indicate that the cytotoxic effect of P. japonica on tumor cells is attributable to the induced apoptosis.

• Nutritional Sciences
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• 제9권1호
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• pp.9-13
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• 2006

The differential effects of various fatty acids such as n-3 and n-6 types or degrees of unsaturation on the CYP2E1 induction and the production of lipid peroxidation (LPO) were investigated. The CYP2E1-transduced human hepatoma HepG2 cells (E47) were cultured in RPMI 1640 media containing different concentrations of various fatty acids up to 48 h incubation compared to 04 cells and CYP2E1-null cells. Treated fatty acids were linoleic acid (LA:n-6, C18:2), arachidonic acid (AA:n-6, C20:4) and docosahexaenoic acid (DHA:n-3, C22:6). The cell survival rate was decreased corresponding to the degree of unsaturation (LA>AA $\cong$DHA) and to LPO production in E47 and 04 cells. The four or five unsaturation degree of fatty acids, AA and DHA, caused time- and dose-dependent cell death in E47 cells but not as much as in C34 (without CYP2E1), suggesting an important role of CYP2E1 in the DHA mediated damage. In the levels of lipid peroxides (LPO), AA also elevated LPO by 3- and 5- fold compared to DHA or LA treated E47 cells. However, AA did not increase LPO until 48 h incubation in C34 cells. In conclusion, the polyunsaturated fatty acids induced CYP2E1 induction might be changed by the elevated levels of lipid peroxide (LPO) and oxidative stress through the connection of CYP2E1 and degrees of unsaturated fatty acids.

• 한국응용곤충학회지
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• 제48권2호
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• pp.197-202
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• 2009

애반딧불이의 유충상륙은 약 5.3일이 소요되었고 고치방을 만든 후 약 6.6일에 번데기로 변태하였다. 고치방의 크기는 길이 10.1 mm, 폭 4.7 mm, 벽두께 1.3 mm이었다. 번데기는 약 10.5일이 소요되었다. 성충이 되고 고치방 속에 약 6.8일 후에 출현하였다. 성충의 출현은 PM. 9시를 전후로 최대였고 PM. 10시에 감소하였다. 성충출현의 최적지온은 $23.4^{\circ}C$였다. 실내개체군(Lab-type)의 암컷 및 수컷과 야외개체군(Nat-type)의 수컷은 수명이 비슷하였고, Nat-type의 산란 수는 200${\sim}$400립 구간을 형성하였다. 단지, Nat-type의 암컷은 수명이 짧았으며, 산란 횟수와 산란 수는 적었다. 산란횟수에서 야외개체군은 2회와 실내개체군은 4회로 차이를 보이는 것은 야외개체군은 시료 채집 이전에 산란했을 가능성을 배제할 수 없다.