• Journal of Life Science
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• v.25 no.8
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• pp.932-935
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• 2015

The purpose of this study was the effect of complex training of obese men in their 30s on inflammatory markers and homocysteine, a risk factor for cardiovascular disease. The subjects consisted of obese men (n=12) with the body fat ratio of 25% or above in their mid 30s who had no medical conditions and can follow the exercise routine required by this study. To achieve the purpose of this study measured the maximum oxygen intake (VO_2max) and 1RM of 5 kinds of machine(bench press, lat pull down, arm curl, leg press, squat) as a preliminary test; based on the results, performed 8 weeks complex training (aerobic exercise + weight training); and then analyzed the variation in body composition (body weight, body fat and BMI), blood inflammatory markers (IL-6, TNF-α and CRP), and homocysteine between before and after training. The results exhibited that 8 weeks complex training reduced weight, body fat and BMI significantly (p<0.01) and also reduced inflammatory marker CRP and homocysteine, a risk factor for cardiovascular disease, significantly (p<0.05). In conclusion, 8 weeks complex training confirmed the variation in body composition, and this variation has a positive effect on the inflammatory marker and the risk factor for cardiovascular disease.

• Journal of the Korean GEO-environmental Society
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• v.5 no.2
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• pp.41-49
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• 2004

In this study, a newly modified soil nailing technology named as the SPN (Spiral Pipe Nailing) system, is developed to self drilling method can apply to ground which is hard to keep shape of bore hole. And limit equilibrium analysis with simplified trial wedge method while length ratio and bond ratio being altered was performed to evaluate slope stability considered of tensile strength and bending stiffness. Also, using $FLAC^{2D}$ program, superiority of the SPN system was compared to the GSN (General Soil Nailing) system about an example section. And effects of various factors related to the design of the SPN system, such as the type of drilling method and the bit, are examined throughout a series of the displacement-controlled field pull-out tests. As a result, the SPN system is better than the GSN system in slope stability because of having larger bending stiffness, tensile strength and unit skin friction. And results of simplified trial wedge method are similar to results of TALREN 97 program, commercial limit equilibrium analysis computer software, about an example section. Consequently, it will find out of that the SPN system reduce displacements and settlements in down excavation process as well as to increase the global stability.

• BMB Reports
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• v.45 no.3
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• pp.183-188
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• 2012

Participates in actin remodeling through Rac and receptor endocytosis via Rab5. Here, we used yeast two-hybrid system with Eps8 as bait to screen a human brain cDNA library. ITSN2 was identified as the novel binding factor of Eps8. The interaction between ITSN2 and Eps8 was demonstrated by the in vivo co-immunoprecipitation and colocalization assays and the in vitro GST pull-down assays. Furthermore, we mapped the interaction domains to the region between amino acids 260-306 of Eps8 and the coiled-coil domain of ITSN2. In addition, protein stability assays and immunofluorescence analysis showed ITSN2 overexpression induced the degradation of Eps8 proteins, which was markedly alleviated with the lysosome inhibitor NH4Cl treatment. Taken together, our results suggested ITSN2 interacts with Eps8 and stimulates the degradation of Eps8 proteins.

• Journal of Life Science
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• v.28 no.7
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• pp.765-771
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• 2018

Peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) is a key transcription factor that regulates adipogenesis, and epigenetic control of $PPAR{\gamma}$ is of great interest in obesity-inhibition research. Our previous study showed that CACUL1 (CDK2-associated cullin domain 1) acts as a corepressor that inhibits $PPAR{\gamma}$ transcriptional activity and adipocyte differentiation. Here, we investigated the roles of protein arginine methyltransferase 5 (PRMT5), a novel binding partner of CACUL1, in regulating $PPAR{\gamma}$. The interaction between PRMT5 and CACUL1 was shown by immunoprecipitation assay in vivo and GST pulldown assay in vitro. As shown by luciferase reporter assay, PRMT5 and CACUL1 cooperated to inhibit the transcriptional activity of $PPAR{\gamma}$. The suppressive role of PRMT5 in adipogenesis was examined by Oil Red O staining using 3T3-L1 cells, which stably overexpress or deplete PRMT5. Overexpression of PRMT5 suppresses $PPAR{\gamma}$-mediated adipogenesis, whereas PRMT5 knockdown increases lipid accumulation in 3T3-L1 cells. Consistently, PRMT5 attenuates the expression of Lpl and aP2, the target genes of $PPAR{\gamma}$, as demonstrated by RT-qPCR analysis. Overall, these results suggest that PRMT5 interacts with CACUL1 to impair the transcriptional activity of $PPAR{\gamma}$, leading to the inhibition of adipocyte differentiation. Therefore, the regulation of PRMT5 enzymatic activity may provide a clue to develop an anti-obesity drug.

• BMB Reports
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• v.40 no.2
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• pp.218-225
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• 2007

In Arabidopsis thaliana, the microtubule-associated protein AtMAP65-1 shows various functions on microtubule dynamics and organizations. However, it is still an open question about whether AtMAP65-1 binds to tubulin dimers and how it regulates microtubule dynamics. In present study, the tubulin-binding activity of AtMAP65-1 was investigated. Pull-down and co-sedimentation exp eriments demonstrated that AtMAP65-1 bound to tubulin dimers,at a molar ratio of 1 : 1. Cross-linking experiments showed that AtMAP65-1 bound to tubulin dimers by interacting with $\alpha$-tubulin of the tubulin heterodimer. Interfering the bundling effect of AtMAP65-1 by addition of salt and monitoring the tubulin assembly, the experiment results indicated that AtMAP65-1 promoted tubulin assembly by interacting with tubulin dimers. In addition, five truncated versions of AtMAP65-1, namely AtMAP65-1 $\Delta$N339 (amino acids 340-587); AtMAP65-1 $\Delta$N494 (amino acids 495-587); AtMAP65-1 340-494 (amino acids 340-494); AtMAP65-1 $\Delta$C495 (amino acids 1-494) and AtMAP65-1 $\Delta$C340 (amino acids 1-339), were tested for their binding activities and roles in tubulin polymerization in vitro. Four (AtMAP65-1 $\Delta$N339, $\Delta$N494, AtMAP65-1 340-494 and $\Delta$C495) from the five truncated proteins were able to co-sediment with microtubules, and three (AtMAP65-1 $\Delta$N339, $\Delta$N494 and AtMAP65-1 340-494) of them could bind to tubulin dimers in vitro. Among the three truncated proteins, AtMAP65-1 $\Delta$N339 showed the greatest activity to promote tubulin polymerization, AtMAP65-1 $\Delta$N494 exhibited almost the same activity as the full length protein in promoting tubulin assembly, and AtMAP65-1 340-494 had minor activity to promote tubulin assembly. On the contrast, AtMAP65-1 $\Delta$C495, which bound to microtubules but not to tubulin dimers, did not affect tubulin assembly. Our study suggested that AtMAP65-1 might promote tubulin assembly by binding to tubulin dimers in vivo.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.65-71
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• 2003

Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces cell death when expressed in yeast. To investigate whether Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various organisms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs in detail. PBI1 is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorho-damine 123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Baxinduced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower levels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. $H_2O_2$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of $H_2O_2$ treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MBP phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to play a novel regulatory role in $H_2O_2$-mediated MAPK signaling in plants.

• The Journal of the Korea Contents Association
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• v.16 no.10
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• pp.503-513
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• 2016

Innovator and Early Adopter are main clients of smart phone until now, but the silver generation enjoying price stability and the Internet emerges, so a strategy of the importance, systematic classification, and focus of a neglected class is needed. It is necessary to analyze UI that can departmentalize not a neglected class limited to the silver generation but potential users who can be voluntarily users and can secure them. To secure them, the researcher analyzes how Windows, Icons, Mouse Pull-Down-Menus(WIMP) of UI has been applied in the process that skeuomorphism UI of iPhone, Metro UI of MS, and Material UI of Google change. As a result of an analysis, skeuomorphism is not entirely new and has secured potential users slowly with a method to compensate and improve a part that users disregard. As a concept of touch UI, Metro UI considered potential users from the beginning but did not secure them with new and epoch-making UI. However, Material UI of Google compensates the weaknesses of skeuomorphism UI and Metro UI and emphasizes cost-effectiveness, so has developed into UI that can secure final consumers of potential users. Therefore, to secure potential users, it should make users themselves understand why they should use smart phone, and it should approach with more organized studies on UI through feedback of observation and direct talks.

• Spatial Information Research
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• v.8 no.1
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• pp.141-153
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• 2000

The purpose of this study is to develop and apply spatial landslide information system using Geographic information system (GIS) in concerned with spatial data. Landslide locations detected from interpretation of aerial photo and field survey, and topographic , soil , forest , and geological maps of the study area, Yongin were collected and constructed into spatial database using GIS. As landslide occurrence factors, slope, aspect and curvature of topography were calculated from the topographic database. Texture, material, drainage and effective thickness of soil were extracted from the soil database, and type, age, diameter and density of wood were extracted from the forest database. Lithology was extracted from the geological database, and land use was classified from the Landsat TM satellite image. In addition, landslide damageable objects such as building, road, rail and other facility were extracted from the topographic database. Landslide susceptibility was analyzed using the landslide occurrence factors by probability, logistic regression and neural network methods. The spatial landslide information system was developed to retrieve the constructed GIS database and landslide susceptibility . The system was developed using Arc View script language(Avenue), and consisted of pull-down and icon menus for easy use. Also, the constructed database can be retrieved through Internet World Wide Web (WWW) using Internet GIS technology.

• Korean Journal of Social Welfare
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• v.67 no.2
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• pp.33-60
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• 2015

The purpose of this study was to analyze the life experiences of fifteen marriage immigrant Asian women who went through running away from their home countries, marrying with Korean men, divorcing from their husbands, and coping with many difficulties after their divorce in Korean society. In order to conduct this study grounded theory methods have been employed. The central phenomenon digged out from this study was 'resistance to baffled reality' (i. e. dislocation). The causal conditions which brought about the central phenomenon were 'escaping for survival' and 'experiencing the gap between reality and expectation. 'The intervening conditions included 'getting to know the reality of their husbands,' 'losing hope,' and 'not being able to pull themselves together.' The contextual conditions consisted of 'being treated as maids,' 'becoming victims of family violence,' 'making up their minds to survive,' 'securing future life,' 'being marginalized,' and 'being aware of themselves as strangers.' The action/interaction strategies on the central phenomenon were 'building support systems,' 'building up will for new life,' and 'reconstructing social identity.'The final outcome was 'arranging places of new settlement.' The divorce was classified as four types: 'coping and growth,' 'emancipation and settling down,' 'being overwhelmed by livelihood,' and 'continuous wandering.' Based on these results, this study provided a few political and practice suggestions to prevent family violence and divorces among multi-cultural families, and also to bumper the impacts of divorce.

• BMB Reports
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• v.41 no.4
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• pp.287-293
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• 2008

In a yeast two-hybrid screen, we identified the microtubule-destabilizing protein SCG10 as a potential effector protein of $BRI_3$. The association was verified using GST pull-down, Co-IP, and their perinuclear co-localization. The analysis of in vitro microtubule polymerization/depolymerization showed that the binding of $BRI_3$ to SCG10 effectively blocked the ability of SCG10 to induce microtubule disassembly, as determined by turbidimetric assays. In intact PC12 cells, $BRI_3$ exhibited the ability to stabilize the microtubule network and attenuate the microtubule-destabilizing activity of SCG10. Furthermore, co-expression of $BRI_3$ with SCG10 attenuated SCG10-mediated PC12 cell neurite outgrowth induced by NGF. These results identify a novel connection between a neuron-specific BRI protein and the cytoskeletal network, suggesting possible roles of BRI3 in the process of neuronal differentiation.