• 한국수산과학회지
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• 제37권3호
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• pp.175-178
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• 2004

We here report that the muscle of 'gukmeri-bok' puffer fish (Takifugu vermicularis radiatus) is intoxicated after freezing and thawing processes even though it is not toxic when it is fresh. This study was carried out to investigate the effect of different freezing and thawing conditions as well as the effect of the presence of skin or viscera on the intoxication of muscle of 'gukmeri-bok' puffer during freezing and thawing process. As a result of this investigation, thawing condition, but not freezing conditions or periods played an important role in muscle intoxication. Tetrodotoxin secretory grands exist in the skin of toxic puffer fish, the skin was more responsible for the muscles intoxication than the viscera during the freezing-thawing process. In other words, no toxicity was measured in the muscle of skinned-frozen specimens even when thawed. According to this result, it is recommended that the skin and viscera must be removed before being frozen for edible purpose. Otherwise, when a whole fish should be frozen, alternative half-thawing and removal of skin from frozen specimens is recommended.

• 한국식품영양과학회지
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• 제39권2호
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• pp.267-273
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• 2010

어육 단백질 급원식품으로서 복어의 활용도를 높이고자 육과 껍질로 분리한 후 이화학적 특성을 살펴본 결과 일반성분은 복육과 복껍질에서 각각 조단백의 함량이 89.5와 82.7%로 가장 높았고, 조지방은 껍질에서 회분은 육에서 더 높은 함량이었다. 핵산관련 물질은 IMP의 함량이 다른 성분에 비해 높았으며, 여타 성분은 $1\;{\mu}mol/g$ 미만으로 정량되었다. 지방산 조성은 포화지방산이 각각 83.9%와 66.3%로 복육에서 더 높았고, 단일불포화지방산인 oleic acid는 복껍질 건조분말에서 25.9%로 복육 건조분말에 비하여 높은 비율이었다. 구성아미노산은 복육 건조분말에서 17종, 복껍질 건조분말에서 16종이 검출되었으며, 총 함량도 복육 건조분말(83,739 mg/100 g)에서 복껍질 건조분말(75,361 mg/100 g)에 비해 더 높았다. 복육 건조분말에서는 glutamic acid가 13,707 mg/100 g으로 가장 높고 다음으로 aspartic acid, lysine, leucine, arginine, alanine, valine, glycine 순이었다. 복껍질 건조분말은 glycine이 14,843 mg/100 g으로 가장 높고, 다음으로 glutamic acid, proline, alanine, arginine의 순으로 높게 나타났다. 유리아미노산은 복육 건조분말에서 25종, 복껍질 건조분말에서 22종이 검출되었는데 taurine이 각각 554.4 mg/100 g과 153.6 mg/100 g으로 가장 높은 함량이었다. 총 유리아미노산의 함량은 복육 건조분말에서 약 1.5배 더 높았으며 특히 cysteine의 경우 복껍질 건조분말에서는 검출되지 않았으나 복육 건조분말에서는 $159.3\pm1.8$ mg/100g으로 정량되었다.

• 한국수산과학회지
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• 제40권5호
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• pp.269-275
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• 2007

The toxicity of two species of puffer fish, Takifugu pardalis and T. niphobles, collected from the coastal regions of Korea was determined using a mouse bioassay. In T. pardalis collected at Tongyeong, the proportion of toxic specimens containing ${\geq}10MU/g$ exceeded 90% for the skin, fins, liver, intestine, ovary, and gallbladder, 11.1% for the testis, and 6.9% for the muscle. In each of the organs, the highest toxin levels were several tens (14-39) of mouse units (MU) per gram in the muscle, testis, and eyeball, but thousands (1,444-5,755) of MU per gram in the skin, liver, intestine, ovary, and gallbladder. The organs of T. pardalis exhibited remarkable variation in toxicity. In T. niphobles, the proportion of toxic specimens exceeded 90% for the ovary and skin, 60-80% for the fins, liver, intestine, and gallbladder, and 4.5% for the muscle; no toxicity was detected in the testis or eyeball using the mouse bioassay. The highest toxin levels were thousands (2,291-7,777) of MU per gram in the liver, intestine, ovary, and gallbladder, hundreds(146-328) of MU per gram in the skin and fins, and 18 MU/g in the muscle. Takifugu niphobles toxicity also exhibited remarkable regional variation. The toxicity in the edible muscle of T. pardalis and T. niphobles was at acceptable levels for human consumption, while the toxicity of the skin of both species of puffer fish was very high, so that care must be taken when used for human consumption.

• 한국수산과학회지
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• 제40권5호
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• pp.276-281
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• 2007

The toxicity of two species of puffer fish, Takifugu xanthopterus and T. stictonotus, collected from coastal regions of Korea, was determined using a mouse bioassay. The highest toxin scores in the muscle, skin, fins, and testis in both species were below 50 mouse units (MU) per gram, and for each organ of both species the proportion of toxic specimens containing ${\geq}10MU/g$ was less than about 10%. In T. xanthopterus, the highest toxin levels in the liver, gallbladder, and ovary exceeded 1,000 MU/g (1,275-1,910), while less than 200 MU/g (12-136) was detected in the same organs of T. stictonotus. Therefore, the toxicities of muscle, skin, and testis in both species of puffer fish were within acceptable levels for human consumption.

• 한국식품위생안전성학회지
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• 제15권1호
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• pp.46-50
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• 2000

우리나라에서 유통되고 있는 자주복의 독성을 조사하기 위하여 1995년 1월, 4월 및 9월에 부산시내 자갈치 어시장에서 총 27개체를 구입하여 부위별 독성을 조사하였다. 간장의 경우 독성이 10MU/g 이상인 유독개체 출현율은 14.8%, 최고 독성치는 160 MU/g, 평균 독성치는 7$\pm$6 MU/g(평균치$\pm$표준오차)이었다. 생식소의 경우는 각각 16.7%, 600 MU/g 및 50$\pm$35 MU/g 이었고, 껍질에서는 각각 14.8%, 26 MU/g 및 5$\pm$1MU/g이었다. 그러나 근육에서는 독이 검출되지 않았다. 계절에 따른 독성은 1월 및 4월의 일부시료에서는 유독부위가 있었으나 9월에는 시료어의 전부위에서 독성이 검출되지 않았다. 자주복의 독성은 부위나 계절에 따른 차이가 있지만 주 가식부인 근육과 껍질에서는 거의 독이 검출되지 않거나 약한 독성이 검출되어 식품 위생상 비교적 안전한 종으로 판단되었다.

• 한국수산과학회지
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• 제42권1호
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• pp.1-7
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• 2009

The toxicity of two species of puffer fish, Takifugu poecilonotus (Heuinjeombok) and T. vermicularis (Gukmaeribok) collected from the coastal regions of Korea was determined using a mouse bioassay. In the T. poecilonotus collected in Jeju and Tongyeong, the proportion of toxic specimens containing ${\ge}10$ mouse units (MU) per gram exceeded 95% for the skin, liver, ovary, and fin, and approximately 30% for the testis and muscles. In each of the organs, the highest toxin levels were 79 MU/g in the muscle, hundreds (158-365) of MU per gram in the fin, intestine, testis, and gallbladder, but thousands (1,147-2,406) of MU per gram in the skin, liver, and ovary. In T. vermicularis collected from Incheon and Gunsan, the proportions of toxic specimens were 100% for the gallbladder, and 56-68% for the skin, fin, liver, and intestine however, no toxic muscle specimens were noted. The highest toxin scores were below 10 mouse units (MU) per gram in the muscle, 20-94 MU/g in the skin and fin, 319 MU/g in the intestine, and thousands (1,548-4,624) of MU per gram in the liver, gonad, and gallbladder. The toxicity in the muscle of T. vermicularis was deemed acceptable for human consumption, whereas the toxicities in the muscle of T. poecilonotus and the skin of both species of puffer fish were significantly high, such that special attention may be required when the fish is intended for human consumption.

• 한국수산과학회지
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• 제41권5호
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• pp.309-314
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• 2008

The toxicity of five species of puffer fish, Arothron firmamentum (Byeolbok), Lagocephalus gloveri (Heukmilbok), L. wheeleri (Eunmilbok), L. inermis (Minmilbok) and L. lunaris (Milbok), collected from fish markets in Korea, was determined using a mouse bioassay. In A. firmamentum, the proportion of toxic specimens containing >10 MU/g was 87.5% in the ovaries, and 10.0% in the skin; no toxicity was detected in the muscle, fin, liver, intestine and gallbladder using the mouse bioassay. The highest toxin levels were found to be 87 MU/g in the ovaries, and 13 MU/g in the skin. Toxic specimens containing >10 MU/g were not detected from samples taken from any of the organs in L. wheeleri and L. inermis. In L. gloveri, most specimens were found to be non-toxic, but toxin levels of 11-72 MU/g were detected from within the skin, fins, and intestines in one specimen. In L. lunaris, the proportion of toxic specimens was 50.0% in the ovaries, and 7.1% in the gallbladder; no toxicity was detected in the other organs by the mouse bioassay. The highest toxin levels were 75 MU/g in the ovaries, and 14 MU/g in the gallbladder. Therefore, the toxicities of edible muscle and skin in the five species of puffer fish marketed in Korea were found to be within acceptable levels for human consumption.

• 한국어병학회지
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• 제20권2호
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• pp.129-137
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• 2007

인공 종묘 생산한 황복 (Takifugu obscurus)자어의 사육 중에 발생한 척추 변형의 원인을 조사하기 위하여 변형어를 병리조직학적으로 조사하였다. 척추 변형은 부화 후 80일령의 황복으로 변형의 발생율은 전체 사육 미수의 약 90%였다. 변형어를 soft-X ray로 촬영한 결과 부레의 팽창은 정상적이었으며, 병리조직학적으로는 변형이 발생한 척추 주변의 근섬유 다발이 소형화되면서 모양도 불규칙하게 변하였다. 그러나 새변 연골은 뒤틀림이나 증생 등은 관찰되지 않았다. 척추 변형이 발생한 종묘 생산장에서는 일반적인 사육 방식보다 1-2일 정도 사육수를 빨리 순환시키면서 급이하고 있었는데, 그 후 황복이 무리를 형성하고 나서, 급이하면서 사육수를 환수한 결과 변형의 발생은 없었다. 병어의 조직학적 변화를 사육방식과 더불어 고찰하였을 때, 유영 능력이 불완전한 황복에 급이하면서 사육수를 환수시킴으로써 황복이 먹이를 섭취하기 위하여 수류에 저항하면서 유영한 것이 척추 변형의 원인으로 작용하였을 것으로 생각한다.

• 한국어병학회지
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• 제18권1호
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• pp.99-103
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• 2005

스쿠티카섬모충에 감염된 자주복의 장, 비장, 신장, 정소, 뇌, 연골 그리고 육질부에 충체의 분포를 조사하였다. 실험어는 사조 CS(주)에서 사육중인 자주복 중 스쿠티카섬모충에 감염된 자주복을 이용하였다. 스쿠티카섬모충은 장, 비장, 신장, 정소, 뇌, 연골, 육질부의 기관을 덮는 결합조직에 분포하고 있다. 뇌에 감염되었을 때 막성조직과 시엽피질 사이가 벌어지는 증상이 있었다. 비장에서 hemosiderin이 과다 침적되는 증상을 제외한 다른 내부 조직과 기관에서는 병리적 증상을 볼 수가 없었다.

• 한국수산과학회지
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• 제39권6호
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• pp.447-453
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• 2006

Toxicity of two species of puffer fish, Takifugu porphyreus and Takifugu rubripes, collected from coastal regions of Korea, was determined using a mouse bioassay, In T. porphyreus, the proportion of toxic specimens containing ${\ge}$ 10 MU/g was 58.3% for the ovary, 32.6% for the skin, 12.0% for the gallbladder, 11.6% for the liver and intestine, and 9.3% for the fin; no toxicity was detected in the muscle and testis using the mouse bioassay. The highest toxin levels were 531 MU/g in the liver, 253 MU/g in the intestine, 136 MU/g in the gallbladder, 118 MU/g in the skin, 116 MU/g in the ovary, and 108 MU/g in the fin. The skin, which is used for human consumption, showed significantly high toxicity with an average of $11{\pm}3\;(mean{\pm}SE) MU/g$. Takifugu porphyreus toxicity also exhibited remarkable regional variation. In T. rubripes, the proportion of toxic specimens was 25.0% for the ovary, 15.8% for the liver, 11.1% for the gallbladder, and 5.3% for the fin and intestine; no toxicity was detected in the muscle, skin, or testis. Among the organs, the highest toxin levels were 228 MU/g in the ovary, followed by 112 MU/g in the liver, 28 MU/g in the gallbladder, 18 MU/g in the intestine, 11 MU/g in the fin, and 8 MU/g in the skin. Thus, we found acceptable toxin levels in the edible muscle and skin of T. rubripes and in the muscle of T. porphyreus. However, the skin of T. porphyreus, which showed significantly high toxicity, requires special attention when used for human consumption.