• Asian Journal of Turfgrass Science
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• v.11 no.1
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• pp.59-72
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• 1997

The influence of respiration on photosythetic electron transport were investigated in the Wid type and psaB mutants from Syneehocystis sp. PCC6803. The amount of glucose uptake in the wild type was proportional to the glucose concentration added in wild type and less than that of psaB mutants in the dark. It was suggested that psaB mutants more depend on the glucose than the wild type. It was investigated how the activities of isocitrate dehydrogenase(IDH) and glucose-6-phos-phate dehydrogenase(G6PDH) were changed. The activities of IDH were very low. While, the ac-tivities of G6PDH were much higher than that of IDH. These results agree to the reports that ex-ogenous glucose was dismilated aerobically via Oxidative Pentose Phosphate Pathway in heterotrophic cyanobacteria. PsaB mutants showed high G6PDH activity in the presence of glucose as well as in the dark and high respiratory activities especially in the dark. It was also investigated how photosynthetic electron transport activities were changed. PsaB mutants showed higher photosynthetic electron tranasport activities than wild type in the presence of glucose as well as in the dark. In the results, it was proposed that photosynthetic electron transport between PS I and PS U was complemented by respiratory electron transport through the NADPH generated by Dxidative Pentose Phophate Pathway in psaB mutant from Synechocystis sp. PCC6803. Key words: Photosynthetic electron transport, Respiration, Synechoystis sp. PCC6803, psaB mutant, Glucose uptake, IDH, G6PDH, Respiratory electron transport activity.

• Journal of Photoscience
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• v.3 no.1
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• pp.23-28
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• 1996

To investigate the structure and function of photosystem I, cartridge mutagenesis technique was used to inactivate the psaB gene of photosystem I. From the screen, many strains which have potential defects in photosystem I were generated. Biochemical analysis revealed that B2, one of the mutant, had a reduced amount of chlorophyll. Electron transfer activitx from photosystem II to photosystem I as oxygen uptake was the rate of 64 % of wild type. Also B2 showed a decreased photosystem I activity when measured by 77 K fluorescence emission spectrum. Particularly, immunodetection analysis showed that the B2 had reduced amount of PsaA/PsaB, but a normal range of PsaC and PsaD. Here we present a photoheterotrophic mutant for psaB gene as a unique model strain for future study of structural/functional relationship and biogenesis of photosystem I.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2835-2838
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• 2013

Background: Prostate cancer features a substantial incidence and mortality burden, similarly to breast cancer, and it ranks among the top ten specific causes of death in males. Objective: To explore the situation of prostate cancer in a healthy population cohort in Eastern Nepal. Materials and Methods: This study was conducted in the Department of General Surgery at B. P. Koirala Institute of Health Sciences, Dharan, Nepal from July 2010 to June 2011. Males above 50 years visiting the Surgical Outpatient Department in BPKIHS were enrolled in the study and screening camps were organized in four Teaching District Hospitals of BPKIHS, all in Eastern Nepal. Digital rectal examination (DRE) was conducted by trained professionals after collecting blood for assessment of serum prostatic specific antigen (PSA). Trucut biopsies were performed for all individuals with abnormal PSA/DRE findings. Results: A total of 1,521 males more than 50 years of age were assessed and screened after meeting the inclusion criteria. The vast majority of individuals, 1,452 (96.2%), had PSA ${\leq}4.0$ ng/ml. Abnormal PSA (>4 ng/ml) was found in 58 (3.8%). Abnormal DRE was found in 26 (1.72%). DRE and PSA were both abnormal in 26 (1.72%) individuals. On the basis of raised PSA or abnormal DRE 58 (3.84%) individuals were subjected to digitally guided trucut biopsy. Biopsy report revealed benign prostatic hyperplasia in 47 (3.11%) and adenocarcinoma prostate in 11 (0.73%). The specificity of DRE was 66.0%with a sensitivity of 90.9% and a positive predictive value of 38.5%. The sensitivity of PSA more than 4ng/ml in detecting carcinoma prostate was 100% and the positive predictive value for serum PSA was 19.0% Conclusions: The overall cancer detection rate in this study was 0.73% and those detected were locally advanced. Larger community-based studies are highly warranted specially among high-risk groups.

• Nuclear Engineering and Technology
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• v.36 no.1
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• pp.83-103
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• 2004

The methodological framework of the Level 2 PSA appears to be currently standardized in a formalized fashion, but there have been different opinions on the way the sources of uncertainty are characterized and treated. This is primarily because the Level 2 PSA deals with complex phenomenological processes that are deterministic in nature rather than random processes, and there are no probabilistic models characterizing them clearly. As a result, the probabilistic quantification of the Level 2 PSA CET / APET is often subjected to two sources of uncertainty: (a) incomplete modeling of accident pathways or different predictions for the behavior of phenomenological events and (b) expert-to-expert variation in estimating the occurrence probability of phenomenological events. While a clear definition of the two sources of uncertainty involved in the Level 2 PSA makes it possible to treat an uncertainty in a consistent manner, careless application of these different sources of uncertainty may produce different conclusions in the decision-making process. The primary purpose of this paper is to characterize typical sources of uncertainty that would often be addressed in the Level 2 PSA and to provide a formal guidance for quantifying their impacts on the PSA Level 2 risk results. An additional purpose of this paper is to give a formal approach on how to combine random uncertainties addressed in the Level 1 PSA with subjectivistic uncertainties addressed in the Level 2 PSA.

• Journal of Life Science
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• v.28 no.9
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• pp.1081-1091
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• 2018

The purpose of this study was to investigate the structure and the glycoconjugate properties of the olfactory mucosa in the rat after inhalation of xylene. Sprague-Dawley male rats were inhalated 300 ppm xylene for 5 times with 5 hours exposure. The olfactory mucosa in the nasal cavity was taken from the animals on 5, 10, 15, 20, and 30 days after inhalation of xylene. The properties of the glycoconjugates in the olfactory mucosa were investigated using 10 biotinylated lectins: (PSA, UEA I, PHA-L, GSL I B4, GSL I, PNA, ECL, SBA, GSL II, and sWGA). In the experimental groups, degenerative changes of the olfactory epithelium were observed until 20 days after inhalation of xylene. In the control group, the olfactory cells in the olfactory epithelium reacted with PSA, UEA I, PNA, SBA, and sWGA, the supporting cells reacted with PSA, PHA-L, GSL I, PNA, ECL, SBA, GSL II, and sWGA, and the Bowman's glands reacted with all 10 lectins. In the experimental groups, the reactivity to PSA, PNA, and SBA in the olfactory cells were decreased, and the reactivity to PSA, PNA, SBA, and GSL II in the supporting cells were decreased. And in the Bowman's gland, the reactivity to PSA, UEA I, GSL I, and sWGA were decreased. Conclusively, the olfactory mucosa was shown a lot of changes in the structure through degenerative process and in the properties of the glycoconjugates after inhalation of xylene. These results suggest that the sugar residues of the glycoconjugates in the olfactory mucosa can be changed by xylene inhalation.

• Research in Plant Disease
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• v.28 no.1
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• pp.26-31
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• 2022

Pseudomonas syringae pv. actinidiae (Psa) is the causal agent responsible for the bacterial canker disease of kiwifruit plants. Psa strains are divided into five different biovars based on genetic and biochemical characteristics. Among them, biovar 2 and 3 strains of Psa were isolated and have been causing widespread damages in Korea. One of the most effective ways to control Psa is to use an antibiotic such as streptomycin. However, Psa strains resistant to this antibiotic were isolated in Korea, and an earlier study revealed that the resistance in the biovar 2 is associated with strA-strB genes. This study aimed to determine the molecular resistance mechanism of Psa biovar 3 strains to streptomycin. Sequencing the rpsL gene encoding ribosomal protein S12 from three streptomycin-resistant strains screened in the laboratory revealed that a spontaneous mutation occurred either at codon 43 or 88. Meanwhile, in four streptomycin-resistant strains of Psa biovar 3 isolated from two kiwifruit orchards, a single nucleotide in codon 43 of the rpsL, which is AAA in streptomycin-sensitive strain, was substituted for AGA causing an amino acid change from lysine to arginine. The resistant mechanism in all biovar 3 strains obtained in Korea was identified as a mutation of the rpsL gene.

• Food Science of Animal Resources
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• v.29 no.2
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• pp.238-244
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• 2009

Food allergy is a serious nutritional problem in both children and adults. Therefore, food allergenicity reduction methods are greatly needed. The allergenicity is altered by various manufacturing processes, and the digestibility of food proteins can be affected by food processing. This study was conducted to investigate the effect of in-vitro digestibility on the allergenicity of autoclaved market pork sausages using pepsin (30min) and trypsin (5, 30, 60, 90, and 120min). The binding ability of the porcine serum albumin (PSA) from sausages A and B significantly decreased by about 30 and 23%, respectively, after autoclave treatment (121; 5, 10, and 30 min). After the pepsin and trypsin treatments, the binding ability of products A and B at 30 min decreased. These competitive indirect enzyme-linked immunosorbent assay (ci-ELISA) results corresponded well with the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting results. The results demonstrated that the allergenicity of pork sausages considerably decreased after autoclave treatment, and were also maintained or decreased after enzyme treatment. Accordingly, autoclave treatment represents a promising processing technology for the reduction of the allergenicity of diverse food products.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.3
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• pp.863-867
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• 2004

Herba Patriniae(HP) has been known to exert anti-inflammation and -tumoral activity in Korea. However, its molecular mechanism of action is not understood. In this study, we found that HP extract induced apoptosis in androgen-dependent prostate cancer LNCaP cells as evidenced by DNA fragmentation. Our data demonstrated that HP extract-induced apoptotic cell death was accompanied by inhibition of NF- κB activation, lowering effects of intracellular prostate specific antigen(PSA) and androgen reoeptor(AR) expression in a time dependent manner. Taken together, HP extract may inhibit the proliferation of prostate cancer LNCaP cell associated with inhibition of NF- κB activation, PSA and AR expression and that of apoptosis.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.3
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• pp.351-361
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• 2012

The leading surgical method for correcting the misalignment of the varus and valgus in the knee joint is the high tibial osteotomy (HTO). In the opening wedge HTO (OWHTO), there is no concern about damaging the peroneal nerve on the lateral tibia of the proximal fibula. OWHTO has been the preferred choice, as the opening of the correction angle can be modulated during the operation. The correction of the varus and valgus on the coronal plane are performed adroitly. Nevertheless, there have been numerous reports of unintended changes in the medial tibial plateau and posterior slope angle (PSA). The authors have developed an HTO method using computer-assisted surgery with the aim of addressing the abovementioned problems from an engineer's perspective. CT images of the high tibia were reconstructed three-dimensionally, and a virtual osteotomy was performed on a computer. In addition, this study recommends a surgical method that does not cause changes in the PSA after OWHTO. The results of the study are expected to suggest a clear relationship between the anteromedial cortex oblique angle of each patient and the PSA, and an optimal PSA selection method for individuals.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 1998.05a
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• pp.383-388
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• 1998

이동통신에서, 신호의 페이딩을 보상하기 위하여 pilot symbol assisted modulation(PSAM)에 대한 많은 연구가 진행 되어왔다. 본 논문에서는 최대비합성다이버시티를 사용하는 PSA-QAM에서 전력증폭기 비선형성의 효과를 분석하였다. 먼저, PSAM성능의 한계를 구하기 위하여, 페이딩에 대한 완벽한 정보가 주어진 상태에서 최대비합성다이버시티를 갖는 QAM의 이론적 성능을 분석하였다. 실제 PSAM에서, 페이딩에 대한 정보는 파일럿 심볼의 보간에 의하여 얻어진다. 오차의 평균전력을 최소화하는 보간필터를 사용하여 필터의 탭수, 파일럿 심볼프레임 주기, 도플러 주파수가 성능에 미치는 영향을 분석하였다. 비선형 전력증폭기 AB, B, C급의 AM/AM, AM/PM 특성을 성능분석에 고려하였다. 비선형 보상기술인 Cartesian Feedback Loop (CFB) 이용하여 AWGN 채널과 Rayleigh 페이딩 채널에서 비선형 전력증폭기 종류에 따른 성능변화를 나타내었다.