• Korean Journal of Community Nutrition
• /
• v.13 no.3
• /
• pp.426-438
• /
• 2008

The purpose of this study was to examine bowel habits, dietary habits, and nutrient intake of constipated adults, and the effects of prune products on relieving constipation symptoms. Fifty one adults with self-reported constipation (mean age 23 years, range 19-41 years, 10 males and 41 females) participated in this study. After a baseline survey on bowel habits and dietuy habits, participants were asked to consume at least 50 g of prune and 200 ml of prune juice per day during a 4-week period in addition to usual diet. Nutrient intake was estimated by a 24 hour recall at the baseline and once every week by diet records during the intervention. Data were analyzed after classifying the subjects into mild constipation group and severe constipation group by the severity of the symptoms. During the intervention, the subjects with mild constipation consumed 56 g of prunes (about 5.6 fruits) and 200 ml of prune juice, and the subjects with severe constipation consumed 59 g of prunes (about 5.9 fruits) and 207 ml of prune juice. Average intakes of energy, dietary fiber and water of the subjects in the mild constipation and severe constipation group increased during the intervention compared to the baseline. Average dietary fiber intake of the mild constipation and severe constipation groups significantly increased from 12.5 g and 11.6 g at the baseline to 18.5 g and 16.8 g after consuming prune products, respectively. These changes were accompanied by an increase in the number of bowel movements, a decrease of defecation time, a change to a softer stool consistency, and a decrease of abdominal pain during defecation. Seventy two subjects answered that prune products were effective to improve their overall constipation symptoms. Our data show that supplementation of prune products is effective to provide energy, dietary fiber and water, and to relieve constipation symptoms for constipated adults.

• Research in Plant Disease
• /
• v.16 no.2
• /
• pp.125-128
• /
• 2010

Bacterial black spot disease of prune fruit (Prunus salicina cv. formosa) has outbroke around major prune production area, Gimcheon, Euiseong and Gunwi in Gyungbuk province and has caused severe economic loss. Integrons PCR primer was designed along with sample pre-incubation and nested PCR method to enhance detection sensitivity for early detection of bacteria in fields. Designed integrons PCR primer successfully detected Xanthomonas arboricola pv. pruni from field collected samples, fruit, leaf, branch and even in raindrop collected from prune orchard. Pre-incubation along with nested PCR enhanced sensitivity to detect X. arboricola pv. pruni from seemingly healthy looking, symptomless branches. Designed integrons PCR can be used in prune nursery fields and in plant quarantine practice for the detection of X. arboricola pv. pruni.

• Proceedings of the Korean Information Science Society Conference
• /
• 2002.10e
• /
• pp.301-303
• /
• 2002

One of the remarkable problems in PIM-DM(Protocol Independent Multicast-Dense Mode) is the latency time, increasing for some periods. The reason of this problem is proved to the confusion of flooding prune message and leave prune message. We propose a convincing solution to this confusion and we prove this by network simulation.

• The Plant Pathology Journal
• /
• v.30 no.2
• /
• pp.178-182
• /
• 2014

Among imported plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose plant quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the plant quarantine in Korea.

• The Plant Pathology Journal
• /
• v.31 no.1
• /
• pp.41-49
• /
• 2015

Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups. While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

• The KIPS Transactions:PartC
• /
• v.10C no.2
• /
• pp.179-184
• /
• 2003

One of the remarkable problems in PIM-DM (Protocol Independent Multicast - Dense Mode) is the join latency time, increasing for specific periods. The reason of this problem is proved to the confusion of flooding prune message and leave prune message. We propose a new solution to this problem, reducing the average join latency by 37.4%, and prove the proposed solution by network simulation.

• Journal of the Institute of Electronics Engineers of Korea TE
• /
• v.37 no.3
• /
• pp.93-103
• /
• 2000

This paper describes Internet multicast routing protocols over MPLS (Multiprotocol Label Switching) networks. Internet multicast routing protocols are divided into 3 categories in terms of tree types and tree characteristics: a shortest path tree, a shared tree and hybrid tree types. MPLS should support various multicast mechanisms because of extremely different IP multicast architectures, such as uni-/bi-directional link, Flooding/prune tree maintenance mechanism, the existence of different tree types with the same group, etc. There are so many problems over MPLS multicast that the solutions can't be easily figured out. In this paper, we make a few assumptions on which the solutions of IP multicast routing protocols over MPLS networks are given. A broadcasting label is defined for the shortest path tree types. Cell interleaving problems of the shared tree types is solved by using block-based transmission mechanism. Finally, the existing hybrid-type multicast routing protocol is reasonably modified to support MPLS multicast.

• The KIPS Transactions:PartC
• /
• v.10C no.1
• /
• pp.77-86
• /
• 2003

This paper describes the new method for Internet multicast routing protocols using MPLS (Multiprotocol Label Switching) networks. Internet multicast routing protocols are divided into three categories in terms if tree types and tree characteristics : a shortest path tree a shared tree and hybrid tree types. MPLS should support various multicast mechanisms because of extremely different IP multicast architectures, such as uni-/bi-directional link, Flooding/prune tree maintenance mechanism. the existence of different tree types with the same group, etc. There are so many problems over MPLS multicast that the solutions can't be easily figured out. In this Paper, we make a few assumptions on which the solutions of IP multicast routing protocols over MPLS networks are given. A broadcasting label is defined for the shortest path tree types. Cell interleaving problems of the shared tree types is solved by using block-based transmission mechanism. Finally, the existing hybrid-type multicast routing protocol is reasonably modified Shortest Path tree type to support MPLS multicast. It has been shown that these modifications give better performance (transmission delay) than the orignal method.

• Plant Biotechnology Reports
• /
• v.2 no.1
• /
• pp.75-85
• /
• 2008

Prune dwarf virus (PDV) is an Ilarvirus systemically infecting almond trees and other Prunus species and spreading through pollen, among other means. We have studied strategies based on coat protein (cp) gene to block PDV replication in host plant cells. A Portuguese isolate of PDV was obtained from infected almond leaves and used to produce the cDNA of the cp gene. Various constructs were prepared based on this sequence, aiming for the transgenic expression of the original or modified PDV coat protein (cpPDVSense and cpPDVMutated) or for the expression of cpPDV RNA (cpPDVAntisense and cpPDVwithout start codon). All constructs were tested in a PDV host model, Nicotiana benthamiana, and extensive molecular characterization and controlled infections were performed on transformants and their progenies. Transgenic plants expressing the coat protein RNA were able to block the proliferation of a PDV isolate sharing only 91% homology with the isolate used for cpPDV cloning, as evaluated by DAS-ELISA on newly developed leaves. With cp expression, the blockage of PDV proliferation in newly developed leaves was only achieved with the construct cpPDV Mutated, where the coat protein has a substitution in the 14th aa residue, with arginine replaced by alanine. This result points to a possible role of the mutated amino acid in the virus ability to replicate and proliferate. This work reveals the possibility of achieving protection against PDV through either coat protein RNA or mutated cp sequence.

• The KIPS Transactions:PartD
• /
• v.12D no.6 s.102
• /
• pp.807-816
• /
• 2005

Sequential pattern mining finds all of the frequent sequences satisfying a minimum support threshold in a large database. However, when mining long frequent sequences, or when using very low support thresholds, the performance of currently reported algorithms often degrades dramatically. In this paper, we propose a novel sequential pattern algorithm using only closed frequent sequences which are small subset of very large frequent sequences. Our algorithm generates the candidate sequences by depth-first search strategy in order to effectively prune. using bitmap representation of underlying databases, we can effectively calculate supports in terms of bit operations and prune sequences in much less time. Performance study shows that our algorithm outperforms the previous algorithms.