• Journal of Microbiology and Biotechnology
• /
• 제18권10호
• /
• pp.1659-1662
• /
• 2008

Pseudomonas chlororaphis O6 exhibits induced systemic resistance (ISR) against P. syringae pv. tabaci in tobacco. To identify one of the ISR metabolites, O6 cultures were extracted with organic solvents, and the organic extracts were subjected to column chromatography followed by spectroscopy analyses. The ISR bioassay-guided fractionation was carried out for isolation of the metabolite. High-resolution mass spectrometric analysis of the metabolite found $C_{9}H_{9}O_{3}N$ with an exact mass of 179.0582. LC/MS analysis in positive mode showed an $(M+H)^{+}$ peak at m/z 180. Nuclear magnetic resonance ($^{1}H,\;^{13}C$) analyses identified all protons and carbons of the metabolite. Based on the spectroscopy data, the metabolite was identified as 4-(aminocarbonyl) phenylacetate (4-ACPA). 4-ACPA applied at 68.0 mM exhibited ISR activity at a level similar to 1.0 mM salicylic acid. This is the first report to identify an ISR metabolite produced by P. chlororaphis O6 against the wildfire pathogen P. syringae pv. tabaci in tobacco.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.81.2-82
• /
• 2003

Plants have evolved along with pathogens, and they have developed sophisticated defense systems against specific microorganisms to survive. G-protons are considered one of the upstream signaling components working as a key for the defense signal transduction pathway. For activation and inactivation of G-protein, GTP-biding proteins are involved. GTP -binding proteins are found in all organisms. Small GTP-binding proteins, having masses of 21 to 30kD, belong to a superfamily, often named the Ras supefamily because the founding members are encoded by human Ras genes initially discovered as cellular homologs of the viral ras oncogene. Members of this supefamily share several common structural features, including several guanine nucleotide binding domains and an effector binding domain. However, exhibiting a remarkable diversity in both structure and function. They are important molecular switches that cycle between the GDP-bound inactive form into the GTP-bound active form through GDP/GTP replacement. In addition, most GTP-binding proteins cycle between membrane-bound and cytosolic forms. such as the RAC family are cytosolic signal transduction proteins that often are involved in processing of extracellular stimuli. Plant RAC proteins are implicated in regulation of plant cell architecture secondary wall formation, meristem signaling, and defense against pathogens. But their molecular mechanisms and functions are not well known. We isolated a RacB homolog from rice to study its role of defense against pathogens. We introduced the constitutively active and the dominant negative forms of the GTP-hinging protein OsRacB into the wild type rice. The dominant negative foms are using two forms (full-sequence and specific RNA interference with RacB). Employing southern, and protein analysis, we examine to different things between the wild type and the transformed plant. And analyzing biolistic bombardment of onion epidermal cell with GFP-RacB fusion protein revealed association with the nucle.

• 한국방사선학회논문지
• /
• 제12권5호
• /
• pp.565-573
• /
• 2018

의료용 사이크로트론은 방사성의약품을 생산하기 위해 양성자를 고속으로 가속시켜 핵반응을 일으키게 되며, 핵반응을 통해 불필요한 중성자가 발생하게 된다. 중성자는 사이클로트론의 부품에 방사화를 일으키는 원인으로 종사자들의 피폭의 원인이 된다. 이에 본 연구에서는 핵반응이 일어나는 Targetry 부품들인 Aluminum body, Silver body, Havar foil의 방사화 정도를 분석하여 피폭선량을 알아보고자 하였다. 실험결과 Aluminum body와 Silver body는 방사화된 핵종들의 에너지가 작고, 반감기가 짧아 종사들에게 미치는 선량이 미미하였으며, 재사용하는데 문제가 없었다. 하지만 Havar foil의 경우 방사화된 핵종들의 에너지가 높고 반감기가 길어 종사자들에게 미치는 영향이 매우 높았으며, 방사성폐기물로써 특별한 관리가 필용한 것으로 나타났다.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 1999년도 Proceedings of International Symposium on Remote Sensing
• /
• pp.208-213
• /
• 1999

Space Physics Sensor (SPS) on-board the KOMPSAT-1 consists of the High Energy Particle Detector (HEPD) and the Ionospheric Measurement Sensor (IMS). The HEPD is to characterize the low altitude high energy particle environment and the effects on the microelectronics due to these high energy Particles. It is composed of four sensors: Proton and Electron Spectrometer(PES), Linear Energy Transfer Spectrometer (LET), Total Dose Monitor (TDM), and Single Event Monitor(SEM). 35MeV proton beam from the medical KCCH cyclotron, at Korea Cancer Center Hospital in Seoul, is used to calibrate the PES. Primary proton beam of 35MeV scattered by polypropylene target is converted to various energy Protons according to the elastic collision kinematics. In this calibration, the threshold level of the proton in the PES can be determined and the energy ranges of PES channels are also calibrated.

• Nuclear Engineering and Technology
• /
• 제22권2호
• /
• pp.108-115
• /
• 1990

0.5~l.2-MeV 양성자빔에 대한 X-선 발생단면적의 측정을 Cu와 Au에 대해 수행하였다. 이 실험에서는 SNU 1.5-MV 탄뎀 반데그라프 가속기에서 얻은 양성자빔을 표적에 입사시켰다. 여기서 방출된 X-선과 후방산란된 양성자는 Si(Li) X-선 검출기와 SSB(Silicone Surface Barrier) 하전입자 검출기를 이용해 동시 측정하였다. 그리하여 측정된 X-선 발생단면적은 다른 실험치들 및 PWBA(Plane Wave Born Approximation)와 ECPSSR(Perturbed Stationary State corrected Energy loss, Coulomb deflection, Relativistic effects)인 이론치들과 비교하였다. 1.0-MeV근처의 양성자에너지에 대해 측정치는 PWBA 값보다는 ECPSSR(D.D. Cohen et al., 1985) 값에 더 잘 일치함을 보였다. 특히, Au의 측정치는 1.2-MeV 양성자빔에 대해 9.69$\pm$0.39 bams이었고, ECPSSR 이론치와는 5% 이내로 일치했으며, 0.5~l.2-MeV 양성자에 대한 실험치는 대부분의 다른 실험치들과 30% 이내로 일치했다.

• 대한의생명과학회지
• /
• 제6권3호
• /
• pp.165-170
• /
• 2000

Vacuolar (H$^{+}$)-ATPase (V-ATPase)는 multi-subunit로 구성된 단백질로서, proton pumping을 통해 세포내 산성화반응에 관여를 한다. 최근에 이 단백질이 synaptic vesicle에서도 발견된 것으로 보아 뇌 신경전달에 중요한 역할을 수행할 것으로 추정하고 있다. 우리는 흰쥐 뇌에서 분리한 mRNA를 주형으로 한 PCR 반응에서 16 kDa subunit의 V-ATPase cDHA를 클로닝할 수 있었고, 이의 염기서열 또한 결정하였다. 분리된 뇌 16 kDa V-ATPase의 coding sequence는 전체 468 bp로서 간에서 보고되었던 것과 동일한 크기였다. 단지 3' 말단의 염기 하나가 A에서 C로 바뀌어 있었는데 모두 alanine (GCA, GCC)을 지정하기 때문에 단백질의 일차구조에는 변화가 없는 것으로 확인되었다. 한편 rat brain cDNA library에서도 동일한 clone이 분리되었는데 역시 같은 부분에서 polymorphism이 발견되었고, RNA splicing 등 더 이상의 조직특이적 변화는 없었다. 본 연구는 16 kDa V-ATPase의 뇌에서의 기능과 신경말단에서의 neurotransmission 및 synaptic vesicle의 재순환 기전을 이해하는데 유용한 정보가 될 것이다.

• 한국진공학회지
• /
• 제20권5호
• /
• pp.367-373
• /
• 2011

동시 계수 도플러 넓어짐 양전자 소멸 분광법으로 p형과 n형 실리콘 시료에 0, 4.0 MeV 에너지의 1.0, $2.0{\times}10^{13}$ protons/$cm^2$ 양성자 빔 조사에 의한 결함을 측정하여 실리콘 구조 특성에 대하여 조사하였다. 양전자와 전자의 쌍소멸로 발생하는 감마선 스펙트럼의 수리적 해석 방법인 S-변수와 W-변수를 사용하여, 시료의 구조 변화를 측정하였다. 본 연구에서 측정된 S-변수는 시료에 조사된 양성자의 빔 에너지에 따라 변하지 않고 거의 일정한 값을 보인 반면, 양성자 조사량의 변화에 따라 결함이 증가하였으며, 그 이유는 양성자 조사 에너지에 따른 Bragg 피크 때문에 에너지는 시료의 특정 깊이에 주로 결함을 형성하여 시료전체에는 결함으로 잘 나타나지 않기 때문으로 판단된다. 그리고 빔의 조사량에 따른 결함의 영향이 큰 것으로 나타났다.

• 한국미생물·생명공학회지
• /
• 제28권2호
• /
• pp.63-70
• /
• 2000

To determine an increased acid tolerance of an adipic acid-resistant mutant Leuconostoc mesenteroides(ANaM100) developed for use as a Kimchi starter, proton permeability of cytoplasm, activities of H+-ATPase, Mg++ release and fatty acid composition of cytoplasmic membranes of strain ANaM100 were studied and compared with those of its wild type (LMw). The value of protons permeability of LMw after an acid shock at pH 5.0 was 5.4 min., while the value of ANaM100 cells was 8.4 min. at the same pH. The pH of maximal specific activ-ities of ATPase originated from the LMw and ANaM100 were 0.87 unit/mg protein at pH 6.0 and 0.92 unit/mg pro-tein at pH 5.5, respectively. The release of magnesium ion from ANaM100 was observed about 12.8% at pH 4 after 2 hours, while the wild strains of LMw released Mg++ about 27.6% under the same conditions. The content of C19:0,cyclo and C18:1 in a membrane fatty acid of ANaM100 was higher and lower, respectively than that of LMw. These results indicated that acid tolerance of adipic acid-resistant strain, ANaM100 was significantly improved in comparison with that of its wild type, LMw. In addition, the strain ANaM100 was adipic resistance based on the result of growth of the strain in comparison with that of strain LMw in a broth containing adipic acid.

• Journal of Astronomy and Space Sciences
• /
• 제32권2호
• /
• pp.145-149
• /
• 2015

A newly designed Tissue Equivalent Proportional Counter (TEPC) has been developed for the CubeSat mission, SIGMA (Scientific cubesat with Instruments for Global Magnetic field and rAdiation) to investigate space radiation. In order to test the performance of the TEPC, we have performed heavy ion beam experiments with the Heavy Ion Medical Accelerator in Chiba (HIMAC), Japan. In space, human cells can be exposed to complex radiation sources, such as X-ray, Gamma ray, energetic electrons, protons, neutrons and heavy charged particles in a huge range of energies. These generate much a larger range of Linear Energy Transfer (LET) than on the ground and cause unexpected effects on human cells. In order to measure a large range of LET, from 0.3 to $1,000keV/{\mu}m$, we developed a compact TEPC which measures ionized particles produced by collisions between radiation sources and tissue equivalent materials in the detector. By measuring LET spectra, we can easily derive the equivalent dose from the complicated space radiation field. In this HIMAC experiment, we successfully obtained the linearity response for the TEPC with Fe 500 MeV/u and C 290 MeV/u beams and demonstrated the performance of the active radiation detector.

• Journal of Astronomy and Space Sciences
• /
• 제31권2호
• /
• pp.159-167
• /
• 2014

Solar variability is widely known to affect the interplanetary space and in turn the Earth's electromagnetical environment on the basis of common periodicities in the solar and geomagnetic activity indices. The goal of this study is twofold. Firstly, we attempt to associate modes by comparing a temporal behavior of the power of geomagnetic activity parameters since it is barely sufficient searching for common peaks with a similar periodicity in order to causally correlate geomagnetic activity parameters. As a result of the wavelet transform analysis we are able to obtain information on the temporal behavior of the power in the velocity of the solar wind, the number density of protons in the solar wind, the AE index, the Dst index, the interplanetary magnetic field, B and its three components of the GSM coordinate system, $B_X$, $B_Y$, $B_Z$. Secondly, we also attempt to search for any signatures of influence on the space environment near the Earth by inner planets orbiting around the Sun. Our main findings are as follows: (1) Parameters we have investigated show periodicities of ~ 27 days, ~ 13.5 days, ~ 9 days. (2) The peaks in the power spectrum of $B_Z$ appear to be split due to an unknown agent. (3) For some modes powers are not present all the time and intervals showing high powers do not always coincide. (4) Noticeable peaks do not emerge at those frequencies corresponding to the synodic and/or sidereal periods of Mercury and Venus, which leads us to conclude that the Earth's space environment is not subject to the shadow of the inner planets as suggested earlier.