• 제목/요약/키워드: proteomic

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Comparative Proteomic Analysis for a Putative Pyridoxal Phosphate-Dependent Aminotransferase Required for Virulence in Acidovorax citrulli

  • Lee, Jongchan;Heo, Lynn;Han, Sang-Wook
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.673-680
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    • 2021

  • Acidovorax citrulli (Ac) is the causative agent of bacterial fruit blotch disease in watermelon. Since resistant cultivars have not yet been developed, the virulence factors/mechanisms of Ac need to be characterized. This study reports the functions of a putative pyridoxal phosphate-dependent aminotransferase (PpdaAc) that transfers amino groups to its substrates and uses pyridoxal phosphate as a coenzyme. It was observed that a ppdaAc knockout mutant had a significantly reduced virulence in watermelon when introduced via germinated-seed inoculation as well as leaf infiltration. Comparative proteomic analysis predicted the cellular mechanisms related to PpdaAc. Apart from causing virulence, the PpdaAc may have significant roles in energy production, cell membrane, motility, chemotaxis, post-translational modifications, and iron-related mechanisms. Therefore, it is postulated that PpdaAc may possess pleiotropic effects. These results provide new insights into the functions of a previously unidentified PpdaAc in Ac.

  • https://doi.org/10.5423/PPJ.NT.09.2021.0139 인용 PDF KSCI

Proteomics에서 계산화학의 역할

  • Gang, Yeong-Gi
    • Journal of Scientific & Technological Knowledge Infrastructure
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    • s.3
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    • pp.62-67
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    • 2000

  • 생물정보학은 유전체 발견과 기능 및 신약설계와 개발 등에 관련된 정보들을 찾아 분석하고 관리하여 유용한 정보를 찾아내자는 학문이다. 특히 3-D 구조에 근거한 신약과 치료법의 개발은 생물정보학에서 아주 중요한 부분이다.

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