• Proceedings of the PSK Conference
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• 2002.10a
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• pp.291.3-292
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• 2002

Cancer is multifaceted disease that presents many challenges to clinicians and cancer researchers searching for more effective ways to combat its often devastating effects. Among the central challenges of this disease, are the identification of markers for improved diagnosis and classification of tumors, and the definition of targets for more effective therapeutic measures. The objective of this study is to identify potential biomarkers for the early detection of gastric cancer in serum. (omitted)

• 대한생식의학회:학술대회논문집
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• 2004.11a
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• pp.88.2-89
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• 2004

• Bulletin of Food Technology
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• v.18 no.1
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• pp.3-15
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• 2005

최근 들어 게놈기능연구는 주요국가의 새로운 국가적 연구표적으로 지정되면서 유전자기반 생물산업의 핵심으로 부각되고 있다. 이러한 발전은인간(2001년 2월 인간 게놈의 초안 발표)을 비롯한 생물체의 게놈구조가 규명되어 이 유전자구조정보를 web상에서 쉽게 알아낼 수 있는데서 비롯된다. 포스트게놈시대의 게놈기능연구를 총괄적으로 '기능유전체학 (Functional Genomics)'이라고하며 여기에는 핵산(DNA나 RNA)을 표적으로 게놈기능을 연구하는 genomics(유전체학, RNA발현을 대상으로 하는 transcriptomics(전사체학) 포함), 총체적인 단백체를 대상으로 유전자기능을 연구하는 proteomics(단백질체학) 및 대사물질을 대상으로 하는 metabolomics(대사체학), 이들 분야를 공통적으로 지원하는 bioinformatics(생물정보학)로 구분된다. 본 고에서는 프로테오믹스 분야를 중심으로 소개하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.10
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• pp.1404-1408
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• 2006

In order to investigate the genetic marker associated with economic performance in Hanwoo (Korean Brown Cattle), proteomic approach was used. Breeding values were estimated from performance tested steers. The top 20 and bottom 19 steers based on carcass weight (CW), eye muscle area (EMA), backfat thickness (BF) and marbling score (MS) evaluation for one progeny testing period was used. Meat samples dissected from longissimus dorsi muscles were taken from the slaughter house and analyzed for two-dimensional gel electrophoresis. A total of 102 significant spots out of total 146 on each gel were detected and compared with the reference gel (synthetic gel) to be evaluated. Four candidate spots for marbling score were identified: 205, 84, 204 and 198. The study confirmed the relationship between breeding values of economic traits of Hanwoo cattle and spot intensity.

• Genomics & Informatics
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• v.14 no.1
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• pp.2-11
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• 2016

The advances in mass spectrometry-based proteomics technologies have enabled the generation of global proteome data from tissue or body fluid samples collected from a broad spectrum of human diseases. Comparative proteomic analysis of global proteome data identifies and prioritizes the proteins showing altered abundances, called differentially expressed proteins (DEPs), in disease samples, compared to control samples. Protein biomarker candidates that can serve as indicators of disease states are then selected as key molecules among these proteins. Recently, it has been addressed that cellular pathways can provide better indications of disease states than individual molecules and also network analysis of the DEPs enables effective identification of cellular pathways altered in disease conditions and key molecules representing the altered cellular pathways. Accordingly, a number of network-based approaches to identify disease-related pathways and representative molecules of such pathways have been developed. In this review, we summarize analytical platforms for network-based protein biomarker discovery and key components in the platforms.

• BMB Reports
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• v.41 no.2
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• pp.108-111
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• 2008

Some proteins of E. coli are stable at temperatures significantly higher than $49^{\circ}C$, the maximum temperature at which the organism can grow. The heat stability of such proteins would be a property which is inherent to their structures, or it might be acquired by evolution for their specialized functions. In this study, we describe the identification of 17 heat-stable proteins from E. coli. Approximately one-third of these proteins were recognized as having functions in the protection of other proteins against denaturation. These included chaperonin (GroEL and GroES), molecular chaperones (DnaK and FkpA) and peptidyl prolyl isomerases (trigger factor and FkpA). Another common feature was that five of these proteins (GroEL, GroES, Ahpc, RibH and ferritin) have been shown to form a macromolecular structure. These results indicated that the heat stability of certain proteins may have evolved for their specialized functions, allowing them to cope with harsh environments, including high temperatures.

• BMB Reports
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• v.46 no.12
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• pp.582-587
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• 2013

Adipocyte differentiation is a complex developmental process forming adipocytes from various precursor cells. The murine 3T3-L1 preadipocyte cell line has been most frequently used in the studies of adipocyte differentiation. Differentiation of 3T3-L1 preadipocytes includes a medium containing fetal bovine serum (FBS) with hormonal induction. In this study, we observed that differentiation medium containing adult bovine serum (ABS) instead of FBS did not support differentiation of preadipocytes. Impaired adipocyte differentiation was due to the presence of a serum protein factor in ABS that suppresses differentiation of preadipocytes. Using a proteomic analysis, alpha-2-macroglobulin and paraoxonase/arylesterase 1, which were previously shown to suppress differentiation of preadipocytes, were identified as anti-adipogenic proteins. Although their functional mechanisms have not yet been elucidated, the anti-adipogenic effects of these proteins are discussed.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.203-205
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• 2002

Light is an environmental signal that regulates photomovement and main energy source of photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803 (Syn6803). Syn6803 is a popular model system for study of plant functional genomics. In this report, we adopted 2D gel based proteomics study to investigate proteins related with the light absorption and photo-protection in Syn6803. More than 700 proteins were detected on the SDS-gels stained with silver nitrate. Several proteins showing different expression level under various light conditions were identified with MALDI-TOF Mass spectrometry. As a comparison, we also conducted ICAT-based proteome study using WT and cphl (cyanobacterial phytochrome 1) mutant. A cphl deletion led to changes in the expression of proteins involved in translation, photosynthesis including photosystem and CO2 fixation, and cellular regulation. We are currently involved in TAP-tagging method to study protein-protein interactions in search for the molecular component involved in the light signal transduction of Syn6803 photomovement.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.558-561
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• 2002

In this study, a method for the localization of pregnancy-specific proteins from cow urine on 2-D gel have been established. The proteins were digested with trypsin in gel and then analyzed with MALDI- TOF or transferred to a membrane and microsequenced. To examine the pregnancy associated protein spot 2 as a diagnosis marker in bovine urine 2-D western blotting was performed. This antibody was reacted specifically in the protein of pregnant cow’s urine. Consequently spot 2 was identified and found to be good candidates for developing cow pregnancy detection assay kit.

• Proceedings of the Korean Society of Crop Science Conference
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• 2007.11a
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• pp.232.1-232.1
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• 2007