• Title/Summary/Keyword: protein digestibility

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Estimation of In vitro Digestibility of Barley Straw by Using a Homogenized Rumen Fluid and Artificial Saliva Mixed with Nitrogen and Energy Sources

  • Chaudhry, Abdul S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.1
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    • pp.13-16
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    • 1998
  • A $2{\times}2{\times}4$ factorial study was conducted to examine the possibility of improving estimates of in vitro digestibility, using untreated (UBS) and ammonia-treated (ABS) barley straw, through homogenization of rumen fluid (RF) and by additions of urea (U) and casein (C) as N sources and Xylose + Glucose (XG) as energy sources into artificial saliva. Digestibiltiy of ABS was significantly greater than that of UBS (p < 0.001). There was a significant decrease in digestibility when additions (U, UC, UCXG) were compared with the control (p < 0.001). A 2-way interaction between RF and straw type was significant (p < 0.05) for dry matter digestibility (DMD). Homogenization of RF increased DMD of ABS (p < 0.05) whereas it decreased DMD of UBS (p > 0.05). The study showed that addition of N alone or in combination with energy sources was not better than control, rather the reverse, digestion was inhibited by a combination of U and casein (UC). It was concluded that sufficient N and branched chain fatty acids were supplied in the inoculum from sheep fed high protein grass cubes to support the growth of cellulolytic microbes during in vitro incubation. Further studies are, however, required to explore the potential of homogenization in improving the in vitro method to estimate digestibility of cereal straws.

Voluntary Intake, Digestibility and Nitrogen Balance in Spotted Deer (Cervus nippon) Fed Forest By-product Silage, Oak Leaf Hay and Commercial Mixed Ration

  • Jeon, B.T.;Moon, S.H.;Lee, S.M.;Kim, K.H.;Hudson, R.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.5
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    • pp.702-705
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    • 2003
  • Forest by-product silage (FPS) comprised of foliage, shrubs, and wild grasses was prepared to examine feeding value as sole roughage source for spotted (sika) deer. This diet was compared with imported oak leaf hay (OLH) and a commercial mixed ration (CMR) in digestion balance trials with spotted deer. Dry matter intake was highest (p<0.05) on the OLH, whereas intakes of the FPS and CMR were similar (p>0.05). Digestibility of dry matter was highest (p<0.05) for CMR. Digestibility of crude protein was highest in CMR and lowest in OLH and there were significant differences (p<0.05) among diets. Average daily gain, digestible dry matter intake and digestibility of crude fiber did not differ significantly among diets. Nitrogen intake, urinary and digestible nitrogen were highest (p<0.05) in CMR. However, nitrogen retention was not significantly different. Consequently, FPS is a good roughage source for deer having high digestibility, dry matter intake and availability of nitrogen.

METHODOLOGICAL ASPECTS OF THE IN VIVO MEASUREMENT OF ILEAL AMINO ACID DIGESTIBILITY IN PIGS - A REVIEW -

  • Yin, Y.-L.;McGracken, Kelvin J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.5
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    • pp.495-502
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    • 1996
  • Much recent research on protein and amino acid (AA) digestive physiology of pigs has been concerned with measurement of the ileal apparent and true digestion and absorption. For measurement of the ileal apparent digestibility of AA, the steered ileo-caecal valve cannulation (SICV) and the ileo-rectal anastomosis (IRA) techniques appear to be the more reliable and simple methods, when compared with any methods requiring use of a marker for calculation of digestibility, or with the complex techniques of ileo-caecal re-entrant cannula (ICRC) and the postvalve ileo-colic re-entrant cannula (IPVC). On the other hand, the peptide alimentation ultrafiltration methods might be a better choice for measurement of the ileal endogenous nitrogen (N) and AA flow in a routine feedstuff analysis, although the classical method of $^{15}N-isotope$ dilution method is still a standard method for N and AA nutrition research in pigs.

Effects of Intake on Digestibility of Grass Silage Harvested at Different Cutting Dates (예취시기를 달리한 Silage의 섭취량이 소화율에 미치는 영향)

  • 성경일;김창주;김동암
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.3
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    • pp.176-179
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    • 1985
  • Digestion trials were conducted to evaluate intake and digestibility of grass harvested at different dates. Silage was prepared from first-growth forage (predominantly timothy) which cutting dates; 4 June (vegetative, called early-cut) and 30 June (heading, called late-cut). The silage was fed to wethers at restricted and ad libitum levels of intake. The crude protein and crude fat of silage decreased with advancing maturity, while contents of fibre increased. DM intake of early-cut and late-cut silage were $99g/W_{kg}^{0.75}\;and\;75g/W_{kg}^{0.75}$, respectively. In digestibility and TDN, significant differences (p<0.01) between early-cut and late-cut silage were found. There was no significant difference in DM digestibility of early-cut silage between restricted and ad libitum feeding, but DM digestibility was decreased markedly in ad libitum feeding that out of account in fecal condition. Digestibility of all composition in early-cut silage, except for hemicellulose, was not observed with increasing levels of intake. Digestibility of crude protein, NDF and hemicellulose in late-cut silage were decreased (p<0.05) in ad libitum when compared with restricted feeding. Results of this study suggest that digestibility of early and late forages harvested as silage varies with levels of intake. Thus, relationship levels of intake and digestibility must be considered cautiously.

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Energy utilization, nutrient digestibility and bone quality of broiler chickens fed Tanzania-type diets in different forms with enzymes

  • Chang'a, Edwin Peter;Abdallh, Medani Eldow;Ahiwe, Emmanuel Uchenna;Al-Qahtani, Mohammed;Mbaga, Said;Iji, Paul Ade
    • Journal of Animal Science and Technology
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    • v.61 no.4
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    • pp.192-203
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    • 2019
  • A study was conducted to determine the influence of feed form and microbial enzyme supplementation on energy utilization, bone quality, and amino acid and mineral digestibility of broiler chickens. Four hundred and eighty Ross 308, day-old broiler chickens were randomly assigned to eight diets formulated from commonly used ingredients in Tanzania. A 2 (pellet or mash) ${\times}$ 4 (control, Axtra XB, Quantum Blue (QB) and Axtra XB + QB enzyme) factorial array in a completely randomized design having six replicates per treatment (10 birds per replicate) was used. Birds were raised in climate-controlled rooms in a 3-phase; starter (0-10 days), grower (11-24 days) and finisher (25-35 days). Apparent metabolizable energy (AME), metabolizable energy intake, net energy of production, energy retained as protein (REp), and efficiency of metabolizable energy use for energy and protein retention were higher (p < 0.05) in birds fed pelleted diets. The AME and REp was higher (p < 0.05) with enzyme supplementation. Ash content, weight, length, width and breaking strength of tibia bones were highest (p < 0.05) in birds on pelleted diets. Tibia bone traits were improved (p < 0.05) when enzymes were included, particularly in a combination of QB and Axtra XB. However, potassium, magnesium, and zinc contents were highest (p < 0.05) when QB was supplemented. Digestibility of all amino acids was higher (p < 0.05) in birds supplied with pellets and with enzyme supplementation for most amino acids, except for serine. There was a positive interaction (p < 0.05) between feed form and enzymes on lysine and phenylalanine digestibility. Digestibility of Ca, P, K, S, Zn, and Fe was higher (p < 0.05) in birds fed pelleted diets, while those on mashed diets had higher (p < 0.05) digestibility of Cu and B. The digestibility of P, K, and Zn was highest (p < 0.001) when QB was added, while Ca, P, S, and B digestibility was highest when a combination of Axtra XB + QB was applied. Pelleted diets with or without enzymes improved energy utilization, digestibility of amino acids, and minerals, and increased bone strength in broiler chickens.

The Effect of Formaldehyde Treatment of Solvent and Mechanical Extracted Cottonseed Meal on the Performance, Digestibility and Nitrogen Balance in lambs

  • Khan, A.G.;Azim, A.;Nadeem, M.A.;Ayaz, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.6
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    • pp.785-790
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    • 2000
  • The effect of formaldehyde treatment of solvent and mechanical extracted cottonseed meal on the performance, digestibility and nitrogen balance was assessed in lambs. Four total mixed rations viz., A, B, C and D containing 40% untreated and treated solvent and mechanical extracted meal were prepared. Sixteen male lambs with average BW of 20-22 kg were randomly allocated to experimental rations and were fed individually during ninety days growth trial. The treatment of solvent extracted cottonseed meal resulted in a linear decrease in ruminal protein degradation. Maximum decrease (64%) in protein degradation was observed at 4 h incubation time with 0.3% formaldehyde treatment. Highest daily BW gain was observed in lambs fed on rations Band D compared to lambs fed on rations A and C. Daily BW gain was higher on rations having 0.3% for fromaldehyde treated cottonseed meals. Higher DM digestibility was observed on ration D compared to other rations. Higher (p<0.05) CP and CF digestibility was observed on rations Band D compared to rations A and C. Nitrogen retention as % age of nitrogen intake was (p<0.05) higher for lambs fed rations B and D compared to rations A and C. Similar pattern was observed for nitrogen retention as percent of nitrogen absorbed. The present study suggested that oil extraction methods of cottonseed did not alter their meal utilization in lambs, however, formaldehyde (0.3%) treatment of meals enhanced its efficiency for growth, digestibility and nitrogen balance in lambs.

Synergistic Effect of Urea and Lime Treatment of Wheat Straw on Chemical Composition, In Sacco and In Vitro Digestibility

  • Sirohi, S.K.;Rai, S.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.7
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    • pp.1049-1053
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    • 1999
  • Chopped wheat straw (0.5-1.5 cm) was subjected to different treatment combinations in a $5{\times}4$ factorial arrangement involving the five levels of urea (0, 2, 3, 4 and 5%, w/w) and four levels of lime (0, 2, 4 and 6%, w/w) at 50% moisture and kept for 3 wk reaction period at about $35{^{\circ}C}$ in laboratory. Treated wheat straw samples were analyzed to study the associative effect of urea and lime on chemical composition, in sacco and in vitro digestibilities. Results showed that cell wall constituents (CWC) solubilized significantly (p<0.01) due to urea and lime treatment on one hand and substantially increase the crude protein (CP) on the other in wheat straw. The main effect on synergism of both chemicals was noticed on organic matter (OM), neutral detergent fibre (NDF), hemicellulose (HC), acid detergent lignin (ADL) and silica by solubilising their contents as a result of considerable increase in cell contents in treated wheat straw. The respective decreases were 5.45, 13.0, 37.23, 44.95 and 26.16% in different treatment combinations. The most interesting feature of the treatment was evident by increase in ash content on each level of lime application. CP content increase up to 12.78% due to urea treatment in comparison with untreated wheat straw (2.56%). The effect of solubilization of structural carbohydrates and increased crude protein due to synergistic effect of urea and lime were clearly seen on improved digestibility of OM and DM. The increase in ISOMD, ISDMD, and IVDMD were 21.67, 21.67, 16.24, and 17.5 units. The increase in digestibility were relative to additions of both chemicals and digestibility values increased with increasing levels of urea plus lime concentration in different treatment combination. The maximum improvement was noticed at 4% urea and 4% lime levels at 50% moisture for 3 wk reaction period in treated wheat straw.

Source of the Variation in Meat and Bone Meal Nutritional Quality

  • Hendriks, W.H.;Cottam, Y.H.;Morel, P.C.H.;Thomas, D.V.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.1
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    • pp.94-101
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    • 2004
  • The gross composition, gross amino acid content, apparent ileal amino acid digestibility and apparent ileal digestible amino acid content from 64 commercially produced meat and bone meals were statistically analysed. The samples were produced by 22 plants over a 2.5 year period with eight plants using batch dry rendering and 14 plants using low temperature rendering. A linear model with method and time of year (period) as fixed effects, plant within method as a random effect and sheep percent as a covariate was fitted to the composition data. The majority of the variation in the gross composition, amino acid digestibility and digestible amino acid content was explained by differences between plants using the same method. Neither rendering season nor origin of the raw materials contributed significantly to the observed variation in meat and bone meal protein quality. Rendering method (low temperature or batch rendering) had a significant effect on the variation observed in gross fat content, gross energy content, pepsin nitrogen digestibility, protein solubility and total lanthionine content. The digestibility of a number of amino acids and the apparent digestible content of arginine, cysteine, aspartic acid, proline and hydroxyproline were also significantly affected by rendering method. On average, batch dry and low temperature rendering systems produce meat and bone meals of similar nutritional quality. The variation between plant and within plant, however, is large, indicating that purchasing meat and bone meal from the same plant does not guarantee a consistent quality.

Effects of Earthworm Meal Supplementation on the Performance of Broiler Chickens (사료 내 지렁이 분말의 첨가가 육계의 생산성에 미치는 영향)

  • 손장호;조익환
    • Korean Journal of Organic Agriculture
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    • v.11 no.2
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    • pp.79-89
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    • 2003
  • A study was conducted to investigate the effect of supplementing earthworm meal on the growth performance and nutrient digestibility of feed in broiler chicks. A total of 120 broiler chicks at 7 days of age were fed the experimental diets containing 0%(Control), 0.2% and 0.4% of earthworm meal for 6 weeks. When fed on both starter and finisher diets, the body weight gain and amount of feed intake were significantly higher (P<0.05) in the chicks fed 0.4% of earthworm meal than those fed 0% and 0.2% of earthworm meal. There were no significantly differences in feed. conversion ratio and mortality among the treatments. When fed on both starter and finisher diets, digestibility of dry matter of feed was not affected by the dietary treatment, but crude fat and crude ash digestibilities of feed were tend to increased in chicks fed earthworm meal Digestibility of crude protein of feed in chicks fed 0.4% of earthworm meal was significantly(P<0.05) improved as compared with those in 0.2% or control group. These results indicated that the dietary supplementations of 0.4% earthworm meal were effective in improve digestibility of crude protein of diet resulted improved broiler performance if.. broiler chicks.

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Effect of feeding a by-product feed-based silage on nutrients intake, apparent digestibility, and nitrogen balance in sheep

  • Seok, J.S.;Kim, Y.I.;Lee, Y.H.;Choi, D.Y.;Kwak, W.S.
    • Journal of Animal Science and Technology
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    • v.58 no.2
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    • pp.9.1-9.5
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    • 2016
  • Background: Literature is lacking on the effects of feeding by-product feed (BF)-based silage on rumen fermentation parameters, nutrient digestion and nitrogen (N) retention in sheep. Therefore, this study was conducted to determine the effect of replacing rye straw with BF-based silage as a roughage source on ruminal parameters, total-tract apparent nutrient digestibility, and N balance in sheep. Methods: The by-product feed silage was composed of spent mushroom substrate (SMS) (45 %), recycled poultry bedding (RPB) (21 %), rye straw (11 %), rice bran (10.8 %), corn taffy residue (10 %), protected fat (1.0 %), bentonite (0.6 %), and mixed microbial additive (0.6 %). Six sheep were assigned randomly to either the control (concentrate mix + rye straw) or a treatment diet (concentrate mix + BF-based silage). Results: Compared with the control diet, feeding a BF-based silage diet resulted in similar ruminal characteristics (pH, acetate, propionate, and butyrate concentrations, and acetate: propionate ratio), higher (p < 0.05) ruminal NH3-N, higher (p < 0.05) ether extract digestibility, similar crude protein digestibility, lower (p < 0.05) dry matter, fiber, and crude ash digestibilities, and higher (p < 0.05) N retention (g/d) Conclusion: The BF-based silage showed similar energy value, higher protein metabolism and utilization, and lower fiber digestion in sheep compared to the control diet containing rye straw.