• 제목/요약/키워드: protein contents

검색결과 4,183건 처리시간 0.035초

인산결핍배지에 있어서의 Chlorella 세포내의 인산화합물의 전환 (Turnover of Phosphate Compounds in Chlorella cells in a P-free medium)

  • 이영녹
    • Journal of Plant Biology
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    • 제9권1_2호
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    • pp.1-6
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    • 1966
  • Using the Chlorella cells which had been uniformly labeled with $^{32}P$, the distribution of phosphorus in various fractions of cell material was investigated. Uniformly $^{32}P$-labeled Chlorella cells were further grown in a P-free medium, and some protions of the cells were taken out at intervals during the culture, and subjected to analyze the contents of $^{32}P$ in various fractins of the cell constituents. 2. Analysis of the $^{32}P$-labeled Chlorella cells showed that the highest in P-content was the fraction of RNA followed by those of lipid, RNA-polyphosphate complex, acid-insoluble polyphosphate, acid-soluble polyphosphate, DNA and protein. 3. During the culture of $^{32}P$-labeled Chlorella cells in a P-free medium, amounts of phosphate in DNA, protein and lipid fractions increased, while the P-contents in the fraction of RNA-polyphosphate complex decreased as well as those of acid-insoluble polyphosphate and acid-soluble polyphosphate fractions. 4. It was inferred that phosphorus used in the syntheses of DNA and protein was taken from polyphosphates of the cells, and RNA-polyphosphate complex would play an important role as a phosphate pool.

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인공 산성비가 중요 농작물의 생육에 미치는 영향 (The Effect of Simulated Acid Rain on the Growth of Important Crops)

  • 허흥욱;허만규
    • 한국환경과학회지
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    • 제7권2호
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    • pp.123-132
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    • 1998
  • The experiment was performed to elucidate the effect of simulated acrid rain on growth of the seven crops(caucus carota vu sativa DC. , Fagopyrum esculenkm Moench, Brassica cmpespis subsp. napus var. pekinensis Makino , Raphuus satlws vu. hortensis for. acnMormis Makino, Brassica dbogjabra Bailey, Caphslcum mum L., and Perilla frutescens Britton). The pH levels of simulated acid rain ranged pH 3.1, 3.6, 4.1, 4.6, 5.1 and 5.6. The germination 10 each crop was influenced from stimulated acrid rain except buckwheat and kale. A general decrease of growth in crops was observed with Increasing pH concentrations. The pattern of soluble protein was observed a tendency to decrease from acidic pH eradlents. According to acidity, total DNA contents of each crop was showed a definite reduction. In conclusion, plant growth was stimulated decreasln and the chanties of total Protein Patterns and BNA contents extracted from leaves trended with stimulated acrid rain was showed seriously.

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식이내 Cadmium과 단백질 수준이 흰쥐의 체내 단백질 대사 및 Cadmium 중독에 미치는 영향 (Effects of dietary Cadmium and Protein Levels on the Body Protein Metabolism and Cadmium Toxicity in Growing Rats)

  • 이혜영
    • Journal of Nutrition and Health
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    • 제21권6호
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    • pp.410-420
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    • 1988
  • This study were performed to investigate effect of dietary cadmium(Cd) and protein levels on growth, body protein metabolism and Cd toxicity in growing rats. Forty eight male rats of Sprague-Dawley weighing 113$\pm$2g were blocked into 6 groups accoridng to body weight. Dietary protein were given at the levels of 7, 15 and 40% of diet and Cd (200ppm)were either added or not. The result obtained were summerized as follow; 1) Food intake, weight gain, FER PER, liver and kidney weight, weight and length of bones, hematocrit, and hemoglobin content in Cd-added groups were low than those in Cd-free groups. 2) Serum total protein showed no significant difference with Cd addition, but it was significantly lower in low protein diet groups. Liver protein in Cd-added groups was lower than Cd-free groups, and was tend to be increased with increasing dietary protein level. 3) Daily urinary and fecal nitrogen excretions in Cd-added groups were lower than Cd-free groups, and were increased with increasing dietary protein level. 4) Cadmium contents in blood, liver, kidney, and femur were tend to be decreased with increasing dietary protein level. Especially, Cd content in kidney of Cd-added groups was significantly decreased with increasing dietary protein level. 5) Daily urinary and fecal Cd excretions were tend to be increased with increasing dietary protein level, and Cd-added-high protein diet group showed the highest Cd excretion among the Cd-added groups, Cd absorption ration and Cd retention ratio were tend to be decreased with increasing dietary protein level.

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돼지에서 분리한 Staphylococcus hyicus subsp hyicus의 protein A (Protein A of Staphylococcus hyicus subsp hyicus isolated from pigs)

  • 김도경;여상건
    • 대한수의학회지
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    • 제30권2호
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    • pp.187-192
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    • 1990
  • 돼지로부터 분리한 Staphylococcus hyicus subsp hyicus 489주의 protein A 존재여부와 함량을 indirect hemagglutination 및 enzyme-linked immunosorbent assay(ELISA)법으로 조사하였다. Indirect hemagglutination text에 의하여 cell-bound protein A 및 extracellular protein A 보유균은 489주 중 각각 87.7% 및 36.0%로 나타났다. ELISA법에 의한 이들 균의 protein A함량 측정에서 전균주의 extracellular protein A는 1ng/ml미만이었으며, cell-bound protein A함량은 대부분의 균주에서 1ng/ml 미만이었고 11주가 25~108ng/ml 수준이었다.

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아토피관련 질병 네트워크로부터 질병단백체 발굴 (Identification of Diseasomal Proteins from Atopy-Related Disease Network)

  • 이윤경;여명호;강태호;유재수;김학용
    • 한국콘텐츠학회논문지
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    • 제9권4호
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    • pp.114-120
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    • 2009
  • 본 연구는 질병과 관련이 있는 단백질들은 질병 네트워크를 형성함에 있어서 매우 중요한 인자로 작용할 가능성이 있다는 아이디어에서 출발한다. 우리는 Online Medelian Inheritance in Man(OMIM)으로부터 아토피관련 43개 단백질 데이터베이스를 확보하고 이 단백질들과 상호작용하는 단백질 네트워크를 구축하였다. 아토피관련 단백질 네트워크를 바탕으로 질병 네트워크를 구축하였다. 질병 네트워크로부터 질병단백체인 CCR5, CCL11, 및 IL4R을 발굴하였는데, 이들 모두는 단백질 네트워크에서 허브 단백질로 작용하는 것들이다. 허브단백질은 세포에서 필수단백질로 작용하는 것으로 알려져 있는데, 본 연구에서는 허브단백질이면서 동시에 질병에서 매우 중요한 역할을 할 것으로 기대되는 질병단백체로 역할하고 있음을 확인하였다. 본 연구에서 소규모 아토피 관련 질병네트워크를 구축하여 분석하였지만, 여기에 제안한 질병네트워크 분석이 복잡한 인간 질병체계의 분자 기작 및 생물학적 진행과정을 이해하는데 실마리를 제공할 것으로 기대한다.

Prediction of Carcass Fat, Protein, and Energy Content from Carcass Dry Matter and Specific Gravity of Broilers

  • Wiernusz, C.J.;Park, B.C.;Teeter, R.G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권1호
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    • pp.42-48
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    • 1999
  • Three experiments were conducted to develop and test equations for predicting carcass composition. In the first study using 52 d-old Cobb ${\times}$ Cobb male broilers, twenty four carcasses were selected from 325 processed birds based upon visual appraisal for abdominal fat (low, medium, high) and assayed for specific gravity (SG), dry matter (DM), fat, protein, and ash. In experiment 2, 120 birds were fed rations containing 2 caloric densities (2,880 and $3,200kcal\;ME_n/kg$ diet) and assayed as described above on weeks 2,3,4,5, and 6. Carcass fat was elevated (p < 0.05) with increased caloric density. In both studies predictive variables were significantly correlated with chemically determined carcass fat, protein, and ash contents. Pooled across the 2 studies, data were used to form SG, DM, and or age based equations for predicting carcass composition. Results were tested in experiment 3, where 576 birds reared to 49-d consumed either 2,880, 3,200, or $3,574kcal\;ME_n/kg$ diet while exposed to constant $24^{\circ}C$ or cycling 24 to $35^{\circ}C$ ambient temperatures. Both dietary and environmental effects impacted (p < 0.05) carcass composition. The fat content analyzed chemically was enhanced from 12.4 to 15.7%, and predicted fat was also elevated from 13.4 to 14.8% with increasing caloric density. Heat distress reduced (p < 0.05) analyzed carcass protein (18.9 vs 18.3%) and predicted protein (18.2 vs 17.5%). Predicted equation values for carcass fat, protein, ash, and energy were correlated with the chemically analyzed values at r=0.96, 0.77, 0.86, and 0.79, respectively. Results suggest that prediction equations based on DM and SG may be used to estimate carcass fat, protein, ash, and energy contents of broilers consuming diets that differ in caloric density (2,800 to $3,574kcal\;ME_n/kg$) and for broilers exposed to either constant ($24^{\circ}C$) or cycling high (24 to $35^{\circ}C$) ambient temperatures during 49-d rearing period tested in the present study.

Bacillus sp. CW-1121이 생성하는 단백 분해 효소를 이용한 참깨박 단백질의 용출 (Extraction of protein from defatted sesame meal using the enzyme from Bacillus sp. CW-1121)

  • 최청;천성숙;조영제
    • Applied Biological Chemistry
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    • 제36권2호
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    • pp.121-126
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    • 1993
  • 참깨박에 함유되어 있는 불용성 형태의 단백질을 가용성 형태의 단백질로 용출시키기 위하여 Bacillus sp. CW-1121이 생성하는 효소를 작용시켰다. 이때 참깨박의 단백질 용출을 위한 최적 pH는 7.5였으며, 최적 온도는 $40^{\circ}C$였고 최적 조건하에서 2시간의 소화로 약 60%의 용해도를 보였다. 단백질의 용해도는 참깨 단백질이 pH4.5에서 가장 낮아 등전점을 보였고, pH3.0과 pH6.0 이상에서는 크게 증가하였다. 효소처리 참깨박 단백질을 전기영동한 결과 수용성 단백질은 4개, 염용성 단백질은 2개의 밴드가 관찰되었다. 분리 단백의 아미노산 조성은 수용성 단백질의 경우 serine이 17.24 mg/g, 염용성 단백질은 glutamic acid가 10.77 mg/g, 유리아미노산은 glutamic acid가 6.55 mg/g으로 가장 많이 함유되어 있었으며 특히 필수아미노산의 조성도 상당히 좋은 편이었다.

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A TMT-based quantitative proteomic analysis provides insights into the protein changes in the seeds of high- and low- protein content soybean cultivars

  • Min, Cheol Woo;Gupta, Ravi;Truong, Nguyen Van;Bae, Jin Woo;Ko, Jong Min;Lee, Byong Won;Kim, Sun Tae
    • Journal of Plant Biotechnology
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    • 제47권3호
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    • pp.209-217
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    • 2020
  • The presence of high amounts of seed storage proteins (SSPs) improves the overall quality of soybean seeds. However, these SSPs pose a major limitation due to their high abundance in soybean seeds. Although various technical advancements including mass-spectrometry and bioinformatics resources were reported, only limited information has been derived to date on soybean seeds at proteome level. Here, we applied a tandem mass tags (TMT)-based quantitative proteomic analysis to identify the significantly modulated proteins in the seeds of two soybean cultivars showing varying protein contents. This approach led to the identification of 5,678 proteins of which 13 and 1,133 proteins showed significant changes in Daewon (low-protein content cultivar) and Saedanbaek (high-protein content cultivar) respectively. Functional annotation revealed that proteins with increased abundance in Saedanbaek were mainly associated with the amino acid and protein metabolism involved in protein synthesis, folding, targeting, and degradation. Taken together, the results presented here provide a pipeline for soybean seed proteome analysis and contribute a better understanding of proteomic changes that may lead to alteration in the protein contents in soybean seeds.

Orchardgrass 및 White Clover의 단파 및 혼파재배에서 붕소의 시용이 목초의 여러 특성에 미치는 영향. III. 목초 중 질소화합물(조/순단백질 및 수용성 질소화합물)의 함량 및 수량 변화 (Effects of Boron Application on the Forage Traits in the Pure and Mixed Cultures of Orchardgrass and White Clover. III. Changes in the contents and yields of N-compounds(crude/pure protein and soluble N-compounds) in forages)

  • 정연규
    • 한국초지조사료학회지
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    • 제23권3호
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    • pp.169-178
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    • 2003
  • Orchardgrass 및 white clover의 단파 및 혼파재배에서 미량요소 붕소(B)의 시비수준별 목초의 생육상, 뿌리/근류 형성, 수량성, 영양성분/무기양분의 함량 및 초종간 경합지수 등에 미치는 영향을 구명하였다. 처리내용은 다양 및 미량요소 시비를 동일하게 한 조건에서 5 수준의 붕소 처리: 1) $B_{0}$ ; 0.0, 2) $B_1$; 0.2, 3) $B_2$; 2.0. 4) $B_3$; 6.0, 5) B$_4$; 15.0me B/pot로 glasshouse에서 시험으로 수행하였다. 본 III보에서는 목초 중 질소화합물(조/순단백질 및 수용성 N-화합물)의 함량 및 이들의 수량성 등에 미치는 영향을 검토하였다. 1. 단파재배에서 white clover는 무추비시 $B_{0}$$B_4$ 처리구는 함량 감소와 N-결핍증상을 보였다. 이에 비해서 $B_2$ 처리구는 이들의 함량이 양호하였으며 추비(특히 N)시보다 순단백질 함량이 더 높았고 이에 따라서 순단백질/수용성 질소화합물의 함량비도 높았다. 추비(특히 N)시에는 B-처리별 조/순단백질의 함량차이가 상대적으로 경미하였다. 2. 혼파재배에서는 추비(특히 N)로 인한 white clover 생육의 열세로 단파재배의 경우와는 다소 다른 B 시비효과를 보였으며, 조/순단백질 함량이 단파보다는 낮았다. 그러나 적합한 B 시비($B_2$)의 시비효과는 단파재배시와 같이 양호하였다. orchardgrass에 대한 붕소의 시용효과는 단파 및 혼파재배 공히 $B_2$, 처리구가 순단백질의 함량 제고에 다소 긍정적 효과를 보였으나 white clover보다 상대적으로 경미하였다. 3. 적합한 B 시비($B_2$)는 white clover의 건물 수량에 미치는 효과보다 조/순단백질의 수량에 미치는 효과가 상대적으로 더 높았다. N-화합물의 함량과 이들의 수량에 미치는 붕소의 시비효과는 초종, 단파와 혼파재배, 그러고 추비의 시비조건에 따라서 차이가 있었다

Optimization for Scenedesmus obliquus Cultivation: the Effects of Temperature, Light Intensity and pH on Growth and Biochemical Composition

  • Zhang, Yonggang;Ren, Li;Chu, Huaqiang;Zhou, Xuefei;Yao, Tianming;Zhang, Yalei
    • 한국미생물·생명공학회지
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    • 제47권4호
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    • pp.614-620
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    • 2019
  • Microalgae have been explored as potential host species for biofuel production. Environmental factors affect algal growth and cellular composition. The effects of several key environmental factors, such as temperature, light, and pH of the medium on the growth and biochemical composition of Scenedesmus obliquus were investigated in this study. The highest growth rate of microalgae was observed at an optimal temperature of 25℃, 150 μmol/(m2·s) light intensity, and pH 10.0. The biochemical composition analysis revealed that the carbohydrate content decreased at lower (20℃) or higher temperature (35℃), whereas the protein and lipid contents increase at these temperatures. The fluctuation of light intensity significantly affected the contents of protein, carbohydrate, and lipid. The protein levels varied greatly when the pH of the medium was below 7.0. The carbohydrate and lipid contents significantly increased at pH above 7.0.