• 한국콘텐츠학회논문지
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• 제5권6호
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• pp.31-40
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• 2005

포스트 게놈 시대에는 단백질에 대한 연구의 필요성이 증대되고 있다. 특히 단백질-단백질 상호작용 및 단백질 네트워크에 대한 연구를 기반으로 전체 생물 체계를 분석하는 연구가 중요하게 대두되고 있다. 기존에 생물학자들이 실험을 통해서 증명한 사실들을 논문이나 기타 매체를 통해서 공개를 하고 있다. 하지만 공개된 정보의 양이 방대하므로 생물학자들이 정보를 효율적으로 이용하지 못하는 경우가 많다. 다행히도 인터넷의 발달로 하루에도 수 없이 쏟아져 나오는 연구 성과들에 쉽게 접근이 가능해졌다. 이러한 매체로부터 생물학적 의미를 가지는 정보를 효과적으로 추출하는 일이 중요하게 대두되었다. 따라서 본 연구에서는 인터넷상에 공개된 다량의 논문 및 기타 정보 매체로부터 단백질 정보를 추출한 데이터베이스로부터 단백질 네트워크를 구성하고, 단백질 네트워크를 통해서 생물학적 의미를 가지는 여러가지 경로 분석 알고리즘을 설계하고 구현한다.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2004년도 추계 종합학술대회 논문집
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• pp.511-515
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• 2004

Post-genome 시대에는 유전체뿐만 아니라 단백질에 대한 연구의 필요성이 증대되고 있다. 특히 단백질-단백질 상호작용 및 단백질 네트워크에 대한 연구를 기반으로 전체 생물 시스템을 분석하는 연구가 중요한 이슈로 떠오르고 있다. 기존에 생물학자들이 실험을 통해서 증명한 사실들을 논문이나 기타 매체를 통해서 공개를 하고 있다. 하지만 공개된 정보의 양이 방대하므로 생물학자들이 정보를 효율적으로 이용하지 못하는 경우가 많다. 인터넷의 발달로 하루에도 수 없이 쏟아져 나오는 연구 성과들에 쉽게 접근이 가능해졌다. 이러한 매체로부터 생물학적 의미를 가지는 정보를 효과적으로 추출하는 일이 중요하게 대두되었다. 따라서 본 연구에서는 인터넷상에 공개된 다량의 논문 및 기타정보 매체로부터 단백질-단백질 상호작용 정보를 추출한 데이터베이스로부터 단백질의 네트워크를 구성하고 단백질 네트워크를 통해서 생물학적 의미를 가지는 여러 가지 경로 분석 알고리즘을 설계하고 구현한다.

• Journal of Nutrition and Health
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• 제19권5호
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• pp.323-332
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• 1986

This study was performed to see the effects of lead poisoning and dietary protein levels(6, 15 and 40 % casein diets) on growth, protein and lipid metabolisms in growing rats. It was also investigated whether the high protein intake would alleviate lead toxicity by decreasing Pb absorption and/or increasing Pb excretion. The results obtained were summarized as follows ; 1) Weight gain, F.E.R liver weight, weight and length of bone in Pb-administered groups were lower than in Pb-free groups. However, these values in the 40% casein diet group with Pb were increased to the level in 15% casein diet group without Pb. 2) Hematocrt and hemoglobin content in blood were lower in Pb -adminstered groups than in Pb free groups. Especially, these levels were lower in 6% casein diet group with Pb than in any other group. 3) Plasma protein level in th e 40% casein casein diet group was the highest of all groups and those of Pb-administered groups tended to be lower than those of Pb-free groups. Plasma lipid and cholesterol levels were increased with decreasing dietary protein level, and these levels were higher in the animals exposed to Pb than in free groups.4) Total liver protein, lipid and cholesterol contents were increased with increasing dietary protein level, and these contents were lower in Pb-administered groups than in Pb free groups. 5) Fecal Pb excretion was not different between 6 and 40% casein diet groups. However, urinary Pb excretion was higher in the 40% casein diet group than in the 6% casein diet group. Above results suggest that, in exposing to the Pb pollution, sufficient protien intake must be recommended. High protein intake seemed to alleviate lead toxicity by increasing urinary Pb excretion.

• 한국작물학회지
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• 제46권5호
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• pp.401-406
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• 2001

The applicability of non-destructive near infrared reflectance spectroscopic (NIRS) method was tested to determine the protein and oil contents of intact soybean [Glycine max (L.) Merr.] seeds. A total of 198 soybean calibration samples and 101 validation samples were used for NIRS equation development and validation, respectively. In the developed non-destructive NIRS equation for analysis of protein and oil contents, the most accurate equation was obtained at 2, 8, 6, 1(2nd derivative, 8 nm gap, 6 points smoothing, and 1 point second smoothing) and 2, 1, 20, 10 math treatment conditions with Standard Normal Variate and Detrend (SNVD) scatter correction method and entire spectrum (400-2500 nm) by using Modified Partial Least Squares (MPLS) regression, respectively. Validation of these non-destructive NIRS equations showed very low bias (protein: 0.060%, oil: -0.017%) and standard error of prediction (SEP, protein: 0.568 %, oil : 0.451 %) as well as high coefficient of determination ($R^2$, protein: 0.927, oil: 0.906). Therefore, these non-destructive NIRS equations can be applicable and reliable for determination of protein and oil content of intact soybean seeds, and non-destructive NIRS method could be used as a mass screening technique for selection of high protein and oil soybean in breeding programs.

• 한국연초학회지
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• 제15권1호
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• pp.75-89
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• 1993

Effect of the amount of fertilizer, plant density, and harvesting time on the production of tobacco leaf protein and fresh biomass was investigated. Flue-cured tobacco(M tabacum, L., cv. NC 82) seedlings were transplanted in the field dressed 200kg N per ha at 1$\times$105, 3$\times$105, 5$\times$105, 7$\times$105 plants per ha, and were harvested at the time when 6 and 10 weeks after transplanting, respectively. Harvest at 10 weeks after transplanting increased greatly number of leaves per plant and fresh weight of a plant, Precentage of senescent leaf weight, but significantly decreased fresh weight of a leaf and total protein contents g-1 fresh weight of leaf and stalk over the amount obtained from the harvest at 6 weeks after transplanting. Also, fresh leaf numbers of a plant, fresh weight of a leaf and of a plant, and total protein contents g-1 fresh weight of biomass were more decreased, but percentage of senescent leaf weight were remarkably increased under higher plant density. Therefore, it was seemed that harvesting at 6 weeks after transplanting under 1$\times$105 plant density per ha is more effective for producing higher yield of biomass and protein per plant than 10 weeks harvesting with 7$\times$105 population per ha. A trend was observed that biomass and protein yields per ha are positively correlated with plant population. Biomass yield per ha was the greatest at 7$\times$105 density(80.5t), but the peak of protein yield was at the near of 5$\times$105 population(2454kg as total protein) per ha on the regression curve. It was assumed that if tobacco plants are transplanted under 5$\times$105 plant density at the mid of May, and thereafter harvest at 6 weeks repeatedly during the growing season, it is possible to harvest 2~3 times per year, and to yield more 6.024kg of protein and over 140me1ric tons of fresh biomass ha 1 year 1 statistically in the korea tobacco growing regions.

• Journal of Biosystems Engineering
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• 제34권4호
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• pp.269-277
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• 2009

In this research, the vegetation growth and rice taste of the liquid fertilizer applied fields (LF) were compared with those of chemical fertilizer applied fields(CF) in order to confirm the possibility of methane fermentation digested sludge as liquid fertilizer using precision agriculture and remote sensing technology. In panicle initiation stage, the vegetation growth at LF was 60%~80% of it at CF and there were significant difference of nitrogen contents between CF and LF. The estimation model of nitrogen contents was established by GNDVI (R=0.607, RMSE=$1.04\;g/m^2$, n=36, p<0.01). In heading stage, vegetation growth at LF went close to it at CF as ratio of 80%~95%. The nitrogen content estimation model was also established (R=0.650, RMSE=$1.73\;g/m^2$, n=35, p<0.01) and there were significant difference of spatial variability between LF and CF. There were not significant difference of rice taste and it's elements, when three samples, which were more than twice of standard deviation, were excepted. The protein contents estimation model using GNDVI of before harvesting (R=0.700, RMSE=0.470%, n=29, p<0.01) were more suitable to predict the protein contents at harvesting comparing with it of heading stage(R=0.610, RMSE=0.521%, n=29, p<0.01).

• 한국식품과학회지
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• 제23권5호
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• pp.627-632
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• 1991

본 실험에서는 국산 유아용 조제분유의 열처리 정도를 파악하기 위하여 HMF 함량, lactulose 함량, 유청단백질 변성율 등을 측정하였다. 조제분유의 HMF 함량은 $21.0{\sim}43.9{\mu}mol/l$로 분말상의 경우 각종 영양성분의 함량이 더 강화된 (ii)단계 제품에서 많은 HMF 함량을 보였으나, 액상의 경우 can 포장용 제품에서 더 많았다. 조제분유의 lactulose 함량은 분말상에서 $2.5{\sim}11.4mg/100ml$였고 액상에서 $27.0{\sim}164.8mg/100ml$으로 제품의 형태에 따라 많은 차이가 있었다. ADPI에서 제시한 열처리 등급을 기준으로 분류했을 때 대부분의 조제분유 제품이 medium-heat 등급에 해당되었으며, 조제분유의 유청단백질 열변성율을 측정한 결과, 분말상에서는 $1.1{\sim}69.4%$였고 액상에서는 $37.4{\sim}71.3%$이었다.

• 한국식품조리과학회지
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• 제33권1호
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• pp.72-77
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• 2017

Purpose: Lycium ruthenicum Murr. is a nutritional food that has been used widely for treatment of heart disease, abnormal menstruation, and menopause. Methods: In this study, the crude protein, crude lipid and crude ash contents of two different Lycii fruits with different colors were investigated, and their color values, total sugar, pH, total anthocyanins and total carotenoids were analyzed. Results: Regarding crude ash, crude fat and crude protein contents, the L. barbarum showed higher in crude fat and crude protein contents than black fruits, whereas L. ruthenicum showed higher contents than black fruits. Regarding mineral composition, mineral contents were in the other of K, Mg, Mn, Na, Zn, and Fe. The K content was high in all of the samples, and the contents of Cr and Cu were not measured. The pH values of L. ruthenicum and L. barbarum were $5.00{\pm}0.01$ and $5.08{\pm}0.02$, respectively. The total sugar content of L. ruthenicum was 45.45% while that of L. barbarum was 45.43%. Ascorbic acid content of L. barbarum was $50.86{\pm}3.63%$ while that of L. ruthenicum was $6.3{\pm}1.40%$. The total anthocyanin content of L. ruthenicum was $462.22{\pm}0.41mg/100g$, although no anthocyanin was detected in L. barbarum. The total carotenoids content was $812.25{\pm}6.01mg/100g$ in L. barbarum, although that of L. ruthenicum was not measured. Conclusion: The results from this study indicate that there is a large difference in the composition of functional ingredients of L. ruthenicum and L. barbarum. There is a strong possibility of L. ruthenicum to be developed into color food sources.

• Journal of Nutrition and Health
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• 제28권2호
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• pp.144-151
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• 1995

This study investigates the supplementing effects of milks by various heat treatment on growth performance and protein and calcium metabolism of rats. For 4 weeks, raw, LTLT-HTST-and UHT-processed milks were given to rats which fed on a calcium free, semi-synthetic diet containing 5%casein. There were no significant differences among the experimental groups in weight gain, feed efficiency ratio and the serum level of total protein and calcium. Also, no significant differences were showed in protein efficiency, nitrogen balance, apparent protein digestibiltiy and the contents of weight and calcium of the left femur as well as 2 incisors. However, the biological value of protein in the UHT-milk group was significantly higher than that of the raw-milk group. The apparent calcium digestibility and calcium balance in the UHT-milk group were higher than those in the raw-, LTLT- and HTST-milk groups. The weight of left femur in all the groups supplemented with various heat-treated milks was significantly impair the nutritive value of protein and calcium in milk. Futhermore, UHT-processing may improve the bioavailability of protein and calcium in milk.

• Journal of Nutrition and Health
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• 제30권7호
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• pp.840-847
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• 1997

In order to investigate the protein intake of breast-fed infants, we examined sixty infants during the first 5 months of lactation. Human milk intake infants increased during lactation with the respective values of 525, 671, 734, 744 , 765 and 768g/day t 0.5, 1, 2, 3, , 4 and 5 months postpartum. The average protein contents of human milk showed 1.58, 1.38, 1.23, 1.11, 1.08and 1.07g/100g respectively. The protein intake of boys during the first 5 months of lactation averaged 9.11g/day which was higher than the 7.71g/day average for girls. Body weight of infants at birth was 3337g, which increased significantly during lactation. The protein intake per body weight of breast-fed infants in boys was significantly higher than that in girls(p<0.01). Protein intake of breast-fed infants had survey, a revaluation of the protein intake and recommended dietary allowance of protein during early infancy should be considered.