• Journal of Korean Society of Forest Science
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• v.96 no.4
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• pp.425-431
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• 2007

To investigate genetic diversity and PCR detection of Rhizina undulata, PCR detection and sequence analysis of rDNA ITS region of R. undulata in soil were analyzed and developed. The length of partial 18S rDNA from four R. undulata isolates were 1,375 nt. The sequence similarity of R. undulata isolates was 100%. The rDNA ITS regions of R. undulata isolates were 585 nt long. Nucleotide sequencing of the ITS regions showed that PDK-1, PTT-1 and PDJ-9 isolates had 100% sequence identity. But, PDS-5 isolate differed from the three isolates by two nucleotide substitution. R. undulata-specific primers designed by the sequence of ITS region were used in PCR detection of R. undulata. PCR products about 525 bp size, which is specific to R. undulata, were amplified from total DNAs of R. undulata isolates. To assay the sensitivity of PCR detection by R. undulata ITS-specific primer, purely cultured mycelial suspension of R. undulata was serially diluted and mixed with 100g of sterile sandy loam soil, respectively. And then, PCR products of total DNAs extracted from each mycelium-soil mixtures were analysed. The PCR protocol could detected up to 1ng mycelium of R. undulata within 100g of soil.

• The Plant Pathology Journal
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• v.24 no.3
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• pp.309-316
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• 2008

Mycelium of Ophiostoma quercus albino strain cultured in liquid culture media was harvested, lyophilized, and stored for examining biocontrol efficacy against wood discoloration by staining fungi in the laboratory and field conditions. Dry weight of mycelium grown in brown sugar yeast extract broth(BYB) showed 3.8 times higher than that grown in potato dextrose broth(PDB). The optimum culture period in BYB was 4 weeks. In vitality test of the albino strain, the lyophilized mycelium stored in liquid nitrogen($-196^{\circ}C$) or in a refrigerator($4^{\circ}C$) kept the vitality until 13 months after storage; however, the mycelium stored at room temperature lost the vitality completely after 13 months. The mycelium stored in liquid nitrogen or in a refrigerator protected wood chips from the discoloration by pretreating mycelial suspension on pine wood chips. The mycelium stored at room temperature for 7 months also showed complete protection. These results suggest that the lyophilized mycelium have a biocontrol efficacy only if it keeps the least vitality. In the field conditions, both albino strain and $Woodguard^{(R)}$(commercial chemical protectant) showed significant differences(p=0.05) in discoloration rate as compared to the non-treated control when these were treated on the wood logs of Pinus rigida. The albino strain showed better protection than $Woodguard^{(R)}$. Isolation frequency of blue stain fungi from the chips of wood logs treated with the albino strain was 0% at three months after treatment, while that treated with $Woodguard^{(R)}$ was 76.7%. In another experiment, pre-treatment of mycelial suspension on the cut surface of wood logs also showed significant protection from wood discoloration. Spraying of both albino strain on the cut surface and insecticides on the bark also showed relatively good control effects as compared to insecticide alone on the bark or nontreated control.

• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.77-88
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• 1999

This experiment was carried out to compare the cultural characteristics of mycorrizal fungi associated with Pinus species, and to form mycorrhizal association with Pinus thunbergii by artificial inoculation of these fungi. Mycorrhizal fungi tested showed great variations in cultural characteristics. Most fungal isolates was best grown on MP medium, except PDA for Lepista sp.(Ln73/92). Hagem for Rhizopogon rubescens(FRI91017), and FDA for Paxillus sp.(Pa60/92). Optimum temperature for these fungi was $25^{\circ}C$, except $30^{\circ}C$ for Pisolithus tinctorius(FRI91004 and Pt1). The range of pH conditions favorable for these fungal isolates were also variable from weak acidic(pH5) to weak alkalic(pH8). Utilization of the carbon sources for these mycorrhizal fungi was different. Fructose, glucose, and maltose were all utilized well, while xylose was not utilized generally. Mycelial growth on the media supplemented with potassium nitrate was better than those on other media with urea, asparagine, or peptone as a nitrogen source, and the poor growth was observed on the media with urea. Pisolithus tinctorius(Pt1) among 7 mycorrhizal fungi artificially inoculated for the mycorrhizal synthesis on pinus thunbergii seedlings in the test tube containing a mixture of peat moss-vermiculite(2:1, v/v) formed mycorrhizae successfully after 3 months. P. tinctorius formed branched and unbranched roots covered with thick fungal mantle and radiating extemal hyphae. Mycorrhizal root cross-sectioned by hand, stained, and observed by Nomarski interference microscope showed typical characteristics of ectomycorrhizae: fungal mantle on epidermal cells and thick Hartig net hyphae around cortex cells.

• Journal of Forest and Environmental Science
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• v.31 no.4
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• pp.280-287
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• 2015

Soil samples were collected from three depths (0-10 cm, 10-40 cm and 40-80 cm) of two forest sites including one plantation dominated by teak with some other minor species and another degraded natural forest in Cox's Bazar, Bangladesh to compare their soil properties. Some vegetation parameters were also studied. For this study $10{\times}10\;m$ and $2{\times}2\;m$ quadrats were used for the tree and undergrowth parameters, respectively. Soil samples were also collected from these quadrats. Between the two forest types, the highest levels of organic carbon, total nitrogen, available phosphorus, exchangeable bases and cation exchange capacity (CEC) were found in soils of the plantation. The soils were acidic in nature and exchangeable Al concentrations were low. Teak dominated forest plantation had higher soil fertility index (SFI) than the degraded natural forest site. Steps for reforestation and appropriate protection are needed to improve the situation.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.352-359
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• 2015

This study was conducted to evaluate how the special protection area designations of degraded trails effect on the soil and vegetation recovery of degraded trails. The study areas were established on an opened trail and a 16-year closed trail, which was prohibited to enter after the designation as a special protection area for recovery, at Bukhansan National Park. Soil core sampling and measurements of vegetation cover were performed to compare soil and vegetation properties of the trails. Soil bulk density increased and soil water total nitrogen decreased on the opened trail, while no significant differences were found on bulk density, soil water, total nitrogen, acidity, and organic matter on the closed trail. On the opened trail, vegetation cover was seemed to be degraded, because vegetation litter cover ratio was low and barren rock cover ratio was high. On the closed trail, litter rock barren cover ratio of the closed trail was recovered, but only limited recovery was found on vegetation cover by applying environmental damage condition rating class. In conclusion, the closed trail was recovered by designation of special protection area, while difference in recovery progress of soil and vegetation was found. Therefore, designation of special protection area of degraded area should be based on scientific basis of recovery characteristics of the area. In order to improve the effectiveness of special protection area system, further specific standards for special protection area designation and management would be needed, considering ecological and social importance of target areas.

• Journal of Forest and Environmental Science
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• v.19 no.1
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• pp.12-17
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• 2003

The last-instar larval external morphologies of Orthosia carnipennis (Butler) and Pseudaletia separata (Walker) of Hadeninae (Noctuidae) were observed.

• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.10-17
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• 1999

The larval external morphologies of Pandemis heparana (Denis et $Schifferm{\ddot{u}}ller$) and P. cinnamomeana (Treitschke) of Archipini were described.

• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.18-26
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• 1999

The external morphologies of Hypena tristalis Lederer and Bomolocha nigrobasalis Herz larvae of Hypeninae were observed.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2005.09a
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• pp.230-233
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• 2005

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.160-167
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• 2006

Most of the forest fires that occur in South Korea are caused by human. We partitioned South Korea into nine districts and used observed weather data and daily fire occurrence records for the 1994 to 2003 period to develop a human-caused fire occurrence model of South Korea. Logistic regression analysis techniques were used to relate the probability of a fire day to Fine Fuel Moisture Code (FFMC) component of the Canadian Forest Fire Danger Rating System. The probability of the number of fire day was increased as FFMC increased in the nine districts of South Korea.