• Journal of Ginseng Research
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• v.43 no.2
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• pp.179-185
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• 2019

Background: Oxidative stress induces the production of reactive oxygen species (ROS), which play important causative roles in various pathological conditions. Black ginseng (BG), a type of steam-processed ginseng, has drawn significant attention due to its biological activity, and is more potent than white ginseng (WG) or red ginseng (RG). Methods: We evaluated the protective effects of BG extract (BGE) against oxidative stress-induced cellular damage, in comparison with WG extract (WGE) and RG extract (RGE) in a cell culture model. Ethanolic extracts of WG, RG, and BG were used to evaluate ginsenoside profiles, total polyphenols, flavonoid contents, and antioxidant activity. Using AML-12 cells treated with $H_2O_2$, the protective effects of WGE, RGE, and BGE on cellular redox status, DNA, protein, lipid damage, and apoptosis levels were investigated. Results: BGE exhibited significantly enhanced antioxidant potential, as well as total flavonoid and polyphenol contents. ATP levels were significantly higher in BGE-treated cells than in control; ROS generation and glutathione disulfide levels were lower but glutathione (GSH) and NADPH levels were higher in BGE-treated cells than in other groups. Pretreatment with BGE inhibited apoptosis and therefore protected cells from oxidative stress-induced cellular damage, probably through ROS scavenging. Conclusion: Collectively, our results demonstrate that BGE protects AML-12 cells from oxidative stress-induced cellular damages more effectively than WGE or RGE, through ROS scavenging, maintenance of redox status, and activation of the antioxidant defense system.

• The Korea Journal of Herbology
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• v.24 no.2
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• pp.87-92
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• 2009

Objectives : This study was performed to evaluate the neuroprotective effect of water extracts from Nelumbinis semen (NSW) in dopaminergic cells. Methods : We performed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, 2,2-azinobis3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) cation scavenging assay, and determination of total polyphenolic content to examine the antioxidant effects of NSW. We also evaluated the neuroprotective effects against 6-hydroxydopamine (6-OHDA)-induced toxicity using 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide assay (MIT) assay, trypan blue cytotoxicity assay, and nitric oxide assay in SH-SY5Y cells and tyrosine hydroxylase (TH) immunohistochemistry in primary rat dopaminergic neurons. Results : NSW showed $IC_{50}$ values of 184.80 and 92.90 ${\mu}$g/mL in DPPH and in ABTS assays, respectively. NSW showed 1.05% of total polyphenol contents. NSW showed protective effect against 6-0HDA-induced neurotoxicity whereas no influence on cell viability at the concentration of 1${\sim}$50 ${\mu}$g/mL. NSW reduced NO generation while 6-OHDA produced it. Moreover, it protected rat dopaminergic neurons against 6-0HDA at a dose of 1 ${\mu}$g/mL. Conclusions : These results indicated that NSW has neuroprotective effect against 6-0HDA-induced neurotoxicity through antioxidant activity in dopaminergic cell culture.

• Journal of The Korean Society of Integrative Medicine
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• v.11 no.2
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• pp.169-179
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• 2023

Purpose : Although human periodontal ligament stem cells (hPDLSCs) are a supportive factor for tissue engineering, oxidative stress during cell culture and transplantation has been shown to affect stem cell viability and mortality, leading to failed regeneration. The aim of this study was to evaluate the antioxidant and protective effects against cell damage of celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, and the antioxidant signal of hPDLSCs in H₂O₂-induced oxidative stress. Methods : To induce oxidative stress in cultured hPDLSCs, H₂O₂ was used as an exogenous reactive oxygen species (ROS). Dose-dependent celecoxib (.1, 1, 10, or 100 µM) was administered after H₂O₂ treatment. WST-1 assay was used to assess cell damage and western blot was used to observe antioxidant activity of hPDLSCs in oxidative stress. Immunohistochemistry was performed for inverting the localization of the SOD and Nrf2 antibody. Results : We found that progressive cell death was induced in hPDLSCs by H₂O₂ treatment. However, low-dose celecoxib reduced H₂O₂-induced cellular damage and eventually enhanced the SOD activity and Nrf2 signal of hPDLSCs. Oxidative stress-induced morphological change in hPDLSCs included lowered the survival and number of spindle-shaped cells, and shrinkage and shortening of cell fibers. Notably, celecoxib promoted cell survival function and activated antioxidants such as SOD and Nrf2 by positively regulating the cell survival signal pathway, and also reduced the number of morphological changes in hPDLS. Immunohistochemistry results showed a greater number of SOD- and Nrf2-stained cells in the celecoxib-treated group following oxidative stress. Conclusion : By increasing SOD and Nrf2 expression at the antioxidant system, the findings suggest that celecoxib enhanced the antioxidative ability of hPDLSCs and protected cell viability against H₂O₂-induced oxidative stress by increasing SOD and Nrf2 expression in the antioxidant system.

• Proceedings of the Korean Society of Computer Information Conference
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• 2023.01a
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• pp.439-441
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• 2023

National park is one of the important types of protected area management systems established by IUCN and a management model for effective conservation and sustainable use of natural and cultural heritage in countries around the world, and it assumes important roles in conservation, scientific research, education, recreation and driving community development. This study takes Liaohe National Park in China, a typical representative of global coastal wetlands, as a case study, and uses python technology to collect travelogues and reviews of visitors from Mafengwo.com, Ctrip.com, Go.com, Meituan.com and Dianping.com as a source, and the text spans from 2015 to 2022. The results show that wildlife resources, natural landscape with river and sea, wetland ecology and fishing and hunting culture of northern China are fully reflected in the perceptions of visitors to Liaohe National Park. However, there is still much room for improvement in terms of supporting services and facilities, public education and tourists' experience and participation in Liaohe National Park. In this paper, we use python data visualization technology to study the public perception of wetland wildlife as the theme, and grasp the satisfaction, spatial distribution, activity content and emotional tendency of the public in the process of wetland wildlife as the theme, so as to better promote the Liaohe National Park to better carry out the public experience while strictly adhering to ecological protection, and to provide the Liaohe National Park with a better opportunity to This will provide scientific basis for the Liaohe National Park to play a better role in ecological civilization construction and education of ecological civilization awareness.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.279-279
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• 2022

Rice is 34% of the world's population used as a staple food. But the world population is increasing. Food security is not well protected. Improving cultivar development can address food security. Quantitative trait locus (QTL) mapping is a statistical analysis using both phenotypic and genotypic dates. The purpose of QTL mapping is to determine a gene. Increasing grain size is a way to increase yield in rice. Grain size-related genes were mapped using CNDH population obtained by cross-breeding Cheongcheong (Indica) and Nagdong (Japonica) through anther culture. Grain harvested from experimental field of Kyungpook National University in Gunwi in 2021. Genes related to grain length were detected between RM5964-RM12285, RM20924-RM20967 in chromosome 1, 7. LOD score is 5.88 and 5.6. Genes related to grain width was detected between RM289-RM18130 in chromosome 5. LOD score is 4.57. Genes related to grain length/width ratio were detected between RM5459-RM3482, RM5699-RM1211 and RM3838-RM3381 in chromosome 1, 2, 5. LOD score is 3.75, 3.14 and 3.41. 4 genes was detected in chromosome 1 and 2 genes was detected in chromosome 2 and 7 genes was detected in chromosome 5. 2 genes related to grain shape and quality were detected. 4 genes related to grain length were detected. 4 genes related to grain size were detected. 1 gene related to grain size and weight was detected. 2 genes related to grain length and weight were detected. By finding the gene related to grain size, it provides food to people threatened by food security and solves the food shortage.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.37 no.2
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• pp.54-65
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• 2019

China government recently tries to preserve not only the famous national historic-culture cities, but also the small and medium-sized cities due to the damages and destruction of the cities by the rapid economic development, urban expansion and the development of tourism industry. Hence, this study chose 'Zhoucun ancient mall' which is one of the hotness of cultural heritage located in a mid-sized city named 'Zhoucun district Zibo city Shandong province' that has continuously cared historical landscapes by the protective policy regarding the city as a historic-culture resource from '80s, and considered the character of the city's development process on how the historical landscapes were well protected. In fact, the protection policy in Zhoucun ancient mall had been started in early '80s, however, the viable plan was established in 2001 entitled 'Historical block protection planning of Zhoucun Da street'. And then, 'Regulatory plan for the ancient mall site of Zhoucun Da street' established in 2008 has expanded the area of the blocks for protection. However, this plan organized area only block-by-block so that the small blocks couldn't have the unity when expanded. Later in 2015, 'Regulation Organization Plan' hastried to expanded the protect-area to solve the unity problem. As a result of the study, the historical landscape management plan of Zhoucun in Zibo city has been systematically changed in 2001 after. In other words, this 2001's plans established buffer zones for the maintenance of protected areas and the surrounding environment. And then the detailed regulatory plan for 2008 led to a concrete urban management plan. After that, in 2015, it was found that the expansion plan to protect and manage the whole area of Zhoucun disrict is to manage the landscape of the surrounding area including the historical landscape of 'Zhoucun ancient mall'.

• Journal of Bio-Environment Control
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• v.20 no.1
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• pp.21-26
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• 2011

Differences among cultivars, conditions of nutrient solution, nursing materials, and soilless culture systems were investigated to select suitable cultivar and cultivation methods for stable production of summer spinach in the highlands. The 'Quinto' spinach showed the earliest growth and highest yield. For yield increase of summer spinach, optimal solution pH was 6.0, EC was $2.0dS{\cdot}m^{-1}$, and $NH_4-N$ ratio of nutrient solution was 30 percent Stand rate of spinach in nursing seedlings, at 200-cell-tray filled with mixed nursing soil (peat : perlite = 7 : 3), was higher than those grown in urethane sponge and rock-wool plug. Yield was also 18 to 24 percent higher than those in rock-wool plug and urethane sponge. Plant length and yield of spinach in mixed substrate (peat : perlite = 7 : 3) filled nutrient film technique (MSNFT) system were longer of 18 percent, and higher of 9 percent than those in deep flow technique (DFT) system, respectively. However, changes of root zone temperature, pH and EC showed similar pattern with DFT. Therefore, growing 'Quinto' cultivar at 6.0 of pH, $2.0dS{\cdot}m^{-1}$ of EC, 30 percent of $NH_4-N$ ratio, at 200-cell-tray filled with mixed nursing soil, and MSNFT cultivation system, was the best for production of summer spinach in the highlands.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.540-544
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• 2010

The nursery plant quality and flower bud induction of new strawberry cultivars, 'Maehyang' and 'Seolhyang' in forcing culture were evaluated in the highland and lowland region. In order to produce daughter plants, the new cultivars were grown in the open field located at both highland (Daekwallyung, above 800 m sea level) and lowland (Gangneung, above 20 m sea level) region, respectively. The average air temperature at highland during nursery plant propagation period was $5.3^{\circ}C$ lower than those at lowland. The number of daughter plants produced at lowland was 2 times as high as those of highland, presumably due to the higher air temperature. Anthracnose incidence rates of runner plants produced at lowland were 4 to 7% as high as those in highland. Mean temperature in the highland ($23.5^{\circ}C$) during flower bud differentiation treatment was $5.1^{\circ}C$ lower than that in the lowland ($28.6^{\circ}C$). Seedlings produced in highland showed higher C/N ratio and lower T/R ratio compared to those produced in lowland. The average flower bud formation date of the daughter plants grown in highland were advanced by 30 days compared to the date in lowland. Accordingly, highland was supposed to be appropriate region for raising seedlings in forcing culture of the new strawberry cultivars of 'Maehyang' and 'Seolhyang'.

• The Journal of the Convergence on Culture Technology
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• v.6 no.1
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• pp.321-326
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• 2020

This paper is to analyze the language education policy in the context of multilingualism. As the majority of the population are multilingual, language policy should be centered on the multilingual speakers as the norm, and multilingual language policy is the best route which we can follow as a language policy in education. The motivation and legitimacy of the multilingual policies are suggested in terms of 6 different perspectives: identity, sustainability, equity, World Englishes, machine translation, and Universal Grammar (UG). As a model of language policy, the English-Plus (i.e., English+n) policy and similarly the Korean-Plus (i.e., Korean+n) policy are suggested to be the most appropriate language policies in the field of education in America and Korea respectively. These plus policies aim at bilingual fluency in both the native language and other foreign languages that are constitutive of the multilingualism of the country in which the bilingualism is treated as a variant of multilingualism. In a period of convergence and diversity in the 4^th Industrial Revolution, language diversity and multilingual policy should be considered as a right to be protected or as a resource to be conserved rather than as a problem to be solved.

• The Journal of the Convergence on Culture Technology
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• v.6 no.4
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• pp.139-147
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• 2020

The number of deaths from child traffic accidents in Korea is currently higher than the average figure of OECD members, and attention needs to be paid to reduce the school zone accident rate. According to the TAAS statistics of the Traffic Accident Analysis System, the number of deaths from traffic accidents in children (under 12 years of age) in Korea has been steadily decreasing recently, but the number of accidents and injuries has been continuously increasing and decreasing. With the number of accidents and injuries of children in Korea continuing to increase and decrease, the number of traffic accidents, deaths, and injuries of children in the school zone (under 12 years of age) has also been on the rise since 2019. Therefore, we were different from the preceding study (Study on Improvement through School Zone Accident Risk Survey, Study on Improvement of Children's Traffic Safety Facilities through Simulation Program). In order to prevent traffic accidents among school zones, which have the highest accident rate among children, a survey was conducted on traffic safety facilities and safety awareness of drivers. After that, we will analyze the problems and present measures for improving traffic safety in the school zone and improving drivers' safety awareness.