• Journal of the Korean Chemical Society
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• v.39 no.6
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• pp.466-470
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• 1995

• Applied Biological Chemistry
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• v.15 no.2
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• pp.175-180
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• 1972

The chemical composition of amino acids in the rind and fresh of Citrus Junos Sieb was studied and compared with that of Citrus natsudaidai Hayate. The results were summarized as follows. 1. Beth of them contained twenty kinds of amino acids, including three kinds of unknown amino acids. 2. Proline in the rind of Citrus Junos and aspartic acid in the rind of Citrus natsudaidai were the richest of all amino acids but on the contrary. Histidine was the poorest of all amino acids in the rind of them. The content of proline amounted to 16.48 mg/100mg in the rind of Citrus Junos and the content of aspartic acid amounted to 32.18 mg/100mg in the rind of Citrus natsudaidai. 3. Aspartic acid was the richest of all amino acids in the flesh of Citrus Junos and the content of it amounted to 32.68mg/100mg. On the other hand, Proline was the richest of all amino acids in the flesh of Citrus natsudaidai and the content of it amounted to 20.93mg/100mg. But the content of histidine as 1.32 mg/100 mg in the flesh of former and tyrosine as 1.18 mg/100 mg in the flesh of latter were relatively small. 4. In the fruits of Citrus Junos and Citrus natsudaidai, aspartic acid and Proline were rich and histidine was poor in quantity. Generally, Flesh contained more amounts of all kinds of amino acids than those rind and especially glutamic acid was richer, compared with other amino acids in flesh.

• Korean Journal of Environmental Agriculture
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• v.36 no.2
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• pp.97-105
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• 2017

BACKGROUND : Environmental stress has a major effect on the growth and yields of vegetables, and can significantly affect nutritionally important phytochemicals, causing large economic losses. METHODS AND RESULTS : The present study was aimed at exploring the effects of water stress on the carotenoid and proline contents in kale leaves to understand drought tolerance of kale plants. Kale was randomly divided into two groups at 57 days after sowing (DAS). One of the groups was well-watered (WW) and the other was water stressed (WS). Harvesting of kale leaves was started one day after treatment (58 DAS) and continued for 10 days (~67 DAS). We investigated the status of plant growth (leaf number, length, width, fresh weight) of kale throughout the study. Carotenoid (lutein, ${\alpha}-carotene$, zeaxanthin, ${\beta}-carotene$) and proline contents were analyzed by high-performance liquid chromatography (HPLC). Our results showed that the total carotenoid contents ranged from 926.0 to 1,212.0 mg/kg dry wt. (at 3 and 2 days, respectively) in WW treatment and 887.8 to 1,157.4 mg/kg dry wt. (at 10 and 4 days, respectively) in WS treatment. The ratio of individual carotenoid to the total carotenoid contents of kale leaves was 51.4 for lutein, 4.44 for zeaxanthin, 2.76 for ${\alpha}-carotene$, and 41.4% for ${\beta}-carotene$. Total carotenoid contents showed a significant reduction from 7 days (1,037.2 mg/kg dry wt.) to 10 days (887.8 mg/kg dry wt.) in WS treatment. The lutein content did not show a significant difference in WW between 7 and 10 days after treatment but showed a significant difference in WS treatment. The ${\alpha}-carotene$ content showed no significant difference between the treatments. However, zeaxanthin content was higher during 4-10 days and ${\beta}-carotene$ content was lower during 6-10 days in WS than in WW on each harvest day. In WW, the proline content showed no significant difference, but in WS, the proline content started to increase at 7 days and almost doubled in 10 days. CONCLUSION : The marked increase in zeaxanthin and proline contents in kale leaves indicated that the two phytochemicals are associated with drought tolerance in the plant.

• Korean Journal of Environmental Agriculture
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• v.16 no.3
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• pp.245-248
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• 1997

The study was carried out to elucidate the changes of ${\alpha}-amylase$ activity and free proline content for zinc toxicity in two rice cultivars(Ilpumbyeo and Namchunbyeo) during germination and early growth stages. Plant height in all kinetin treatments was promoted but zinc 120ppm treatment was decreased. Soaking treatment of kinetin $10^{-3}M$ increased germination rate of both cultivars, Ilpumbyeo and Namchunbyeo by 95% and 96% as compared with zinc 120ppm. Chlorophyll content of Ilpumbyeo was higher than that of Namchunbyeo. Activity of ${\alpha}-amylase$ in kinetin $10^{-3}M$ of both rice cultivars was most highest in the 3days after treatment of zinc 120ppm. Free proline content in all rice cultivars of zinc 120ppm treatment was sharply increased at the 3days after treatment of zinc 120ppm. As a result, the effects of kinetin treatment were recognized to promote the plant height and germination rate under zinc toxicity(120ppm) during rice seed germination and early growth stages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.182-188
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• 1997

The study was carried out to determine an optimum concentration of plant growth regulators(ABA and kinetin) on reducing NaCl toxicity during germination in rice. Plant height of a japonica cultivar Ilpumbyeo in ABA 10$^{-5}$ M was increased, while all treatments of kinetin had no clear effects on increasing the plant height. However, other rice cultivars showed slightly different reaction by PGRs compared with Ilpumbyeo. Germination rate of four rice cultivars was ranged from 53.1 to 58.2％ in NaCl 1.3％. All treatments of kinetin accelerated germination rate of tested rice cultivars except Dasanbyeo compared with NaCl 1.3％ treatment. The higher concentration of ABA and kinetin treatment induced the higher starch content, and while the lower concentration of two plant growth regulators induced the higher sugar content in all four rice cultivars. The free proline content of rice seedlings was highest in Ilpumbyeo among the tested cultivars under NaCl 1.3％ treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.189-195
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• 1997

To cope with increasing importance of water stress in food crop production, characteristics and cultivar differences in physiological responses of winter barley plants to water stress during seedling stage were studied employing three covered-barley, Milyang 12, Durubori, and Olbori, one naked-barley, Baekdong, and one two-row beer-barley, Hyangmaeg. Water stress was given to barley plants by withholding watering in soil-culture pots or by adding P.E.G. (polyethylene glycol, M. W. 4,000) to the nutrient culture solution. When water stress was imposed by withholding watering for 10 days to the seedlings grown in pot-soil for 10 days after emergence, leaf water potential of 5 cultivars was decreased almost to the same degree, from control -3.5 bar to the water stressed -26.6 bar. However, growth and some physiological responses were differently affected by cultivars: The plant height was decreased by 16-26％ : seedling dry weight by 25∼42％ ; chlorophyll content by 21∼39％ ; second leaf survival rate by 8∼18％ ; and free proline content per gram of leaf dry weight was increased from control 0.2∼0.5mg to the water stressed 5.8∼13.2mg. Drought resistances of 5 barley cultivars, based on the leaf survival rate and the decreased rate in seedling dry weight at the end of stress treatment, were high in the order of 3 covered-barley＞naked-barley＞two-row barley.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.534-538
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• 1997

Some physiological characteristics and cultivar differences of winter barley to salt stress were studied during seedling stage. Salt stress was caused by adding NaCl solution to the pot culture soil. Measurements of the responses to salt stress and of the responses after relief from stress were done in terms of leaf water potential, chlorophyll and free proline contents, seedling height and seedling dry weight, and survival rate of leaves. Under salt stress ($\Psi_{\pi}$ =-20bar) seedling height and seedling weight were decreased by 2~22% and by 25~39% respectively, showing some differences among cultivars. Chlorophyll contents was decreased by 33~49%, and free proline content was remarkably increased from control 0.2~0.3mg to salt stress 9.6~14.7mg. The leaf water potential of seedling grown under salt stress with NaCl solution($\Psi_{\pi}$ =-10 or -20bar) was decreased from control -3.3bar to salt stress -9.0bar or -16.2bar respectively but there were no large differences among cultivars with time after relief from salt stress. Leaf survival rate was high in order of Baegdong, Milyang12, Olbori, Durubori and Hyangmaeg, and decrease rate of seedling dry weight was low in the order of Baegdong, Olbori, Hyangmaeg, Milyang12, Durubori. The increase in free proline contents was high in the order of Milyang12, Hyangmaeg, Baegdong, Durubori and Olbori.

• Journal of Oral Medicine and Pain
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• v.15 no.1
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• pp.91-104
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• 1991

After alkaline slab polyacrylamide gel electrophoresis and 3.3'-DMB staining of parotid saliva from 48 peoples in Ullung-do and 35 peoples in Jawall-do, the author have got following conclusions. 1. The gene frequencies of Proline-rich protein in Ullung-do were Pr1 =0.719, Pr2=0.281 2. The gene frequencies of Proline-rich protein in Jawall-do were Pr1=0.671, Pr2=0.329 3. The gene frequencies of Double band protein in Ullung-do were Db+=0.087, Db-=0.913 4. The gene frequencies of Double band protein in Jawall-do were Db+=0.0.106, Db-=0.0.894 5. The gene frequencies of Pa protein in Ullung-do were Pa+=0.179, Pa-=0.821 6. The gene frequencies of Pa protein in Jawall-do were Pa+=0.167, Pa-=0.833 7. The gene frequencies of Pr1 of Ullung-do and jawall-do were lower than those of Pr1 in Seoul and Onyang, but similar to those of Pr1 in kangnung and Cheju. 8. The gene frequencies of Db- of Ullung-do and Jawall-do were much higher than those of Db+ in Japanese, Chinese and other populations in Korean.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.28 no.1
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• pp.31-41
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• 2002

Proline-rich proteins (PRPs) are major components of human saliva. In order to know the biological roles of PRPs, we explored the expression pattern and functional protein structures of PRPs by the immunohistochemical and various molecular biological methods. Polyclonal antibody against human gPRP was generated from rabbit by the injection of oral exfoliated cells specially treated by urea and SDS buffer. The PRPs began to be expressed both in the acinar cells and ductal cells from the EIDS (Early Intermediate Developmental Stage) of fetal salivary glands and became intense in the salivary epithelium in the LDS (Late Developmental Stage) and adult salivary glands. The polyclonal antibody against the gPRP showed the cross-reactivity with aPRP and bPRP, these results were relevant to the high homology among subtypes of PRP. However, the simulated protein structures of PRPs showed the characteristic repetitive whorling domains except the N-terminal signal peptide. The whorling domains were also contained the multiple amino acids of glutamine and glycine, which may provide the receptor binding or cross-linking sites of PRPs.

• The korean journal of orthodontics
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• v.20 no.2
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• pp.227-245
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• 1990

The purpose of this study was to analyze the reorganization of periodontal ligament after collagenase treatment with autoradiography. The author compared the collagenase-treated experimental group and no-treated experimental group with control group. Fourty eight Sprague-Dawley rats were divided into nine groups, including normal control and immediate group. Closed coil springs were used between the upper incisors and the first molars with 100 grams. Collagenase and $^3H-proline$ were adminstered and the samples were sacrificed and sectioned. After being dipped into the NTB-3 emulsion the samples were analyzed with light microscope under H/E stain. Data were analyzed by t-test and ANOVA. The results were as follows: 1) Generally collagenase-treated groups got more $^3H-proline$ uptake than no-treated groups. 2) Compared with normal control group, collagenase-treated group had the same $^3H-proline$ uptake in amount at 21th day. 3) Among cemento-enamel junction, middle, apex areas, cementa-enamel junction area of collagenase-treated group arrived at normal control level earlier than no-treated group. 4) Cemento-enamel junction area had the most $^3H-proline$ incorporation amount in no-treated group, but apex area had the most in collagenase group.