• Title/Summary/Keyword: program similarity

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Cloning of Two chitin Synthase Gene Fragments from Penicillium diversum (Penicillium diversum으로부터 두 chitin synthase 유전자 절편의 분리)

  • Cho, Seong-Pil;Lee, Sang-Keun;Lee, Dong-Hun;Bae, Kyung-Sook;Park, Hee-Moon;Maeng, Pil-Jae
    • The Korean Journal of Mycology
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    • v.25 no.3 s.82
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    • pp.167-175
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    • 1997
  • The PCR fragments of two distinct chitin synthase genes, PdCHSl and PdCHS2, were cloned from Penicillium diversum KCTC 6786. The nucleotide sequences of PdCHSl and PdCHS2 contained uninterrupted open reading frames (ORFs) of 570 bp excluding the primer sequence. The similarity analysis of the deduced amino acid sequences using BLASTP indicated that the possible evolutionary relationship between P. diversum and ascomycetous fungi. Multialignment of the deduced amino acid sequences of PdCHSs using CLASTAL W revealed that the PdCHSs fell into two different classes: PdCHSl into Class I and PdCHS2 into Class II of chitin synthase defined by Bowen et al. (1992). By Southern blot analysis, it was shown that each of the two genes is present as a single copy in the genome of P. diversum KCTC 6786.

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Complete Mitochondrial Genome of Crangon hakodatei (Rathbun, 1902) (Crustacea: Decapoda: Crangonidae) (마루자주새우[Crangon hakodatei (Rathbun, 1902)]의 전장 미토콘드리아 유전체에 대한 분석 연구)

  • Kim, Gyungryul;Kim, Hyun-Woo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.6
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    • pp.867-874
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    • 2016
  • Although shrimps belonging to family Crangonidae are known to be genetically divergent and ecologically important among the various benthos, any of their mitochondrial genome has not been reported yet. We here determined the complete mitochondrial genome sequence of Crangon hakodatei (Rathbun, 1902), which was collected from East China Sea ($124^{\circ}E$ and $34.5^{\circ}N$). Total mitochondrial genome length of C. hakodatei was 16,060 bp, in which 13 proteins, 2 ribosomal RNAs, 22 transfer RNAs and a putative control region were encoded. Secondary structure prediction analysis showed that twenty tRNA genes exhibit the conserved structure but two genes, $tRNA^{Cys}$ and $tRNA^{Ser}$ (AGN), lack T and D arm, respectively. Based on the sequence similarity of the COI region from the currently reported five species belonging to genus Crangonidae, C. hakodatei was most closely related to Crangon crangon. Phylogenetic analysis of full COXI genes belonging to infraorder Caridea showed that only crangonid shrimps were clustered together with those of Dendrobranchiata. Gene order were well conserved from Penaeoidea to Caridea but $tRNA^{Pro}$ and $tRNA^{Thr}$ in Palaemonid shrimp were flipped each other by the recombination. Further study about mitochondrial genome sequences of shrimps belonging to Crangonidae should be made to know better about their evolutional relationships with other those in infraorder Caridea.

A Study on the Method of Deriving Emotional Images of Digital Materials Using KES-FB Hand Evaluation Data (KES-FB 태 평가 데이터를 활용한 디지털소재 감성이미지 도출방법 연구)

  • Yoon, Hye Jun
    • Fashion & Textile Research Journal
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    • v.23 no.5
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    • pp.667-673
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    • 2021
  • The purpose of this study was to obtain drape information and objective texture of fabrics easily and quickly by using a constructed fabric database. For the construction of the fabric database, 287 woven fabrics were examined by using the CLO fabric kit, KES-FB system, and drape test system. The k-means cluster analysis method was used to classify the fabrics into 7 grades. After correlation analysis of the fabric properties for each experiment, similar properties of the CLO fabric kit and KES-FB system were chosen, which were then designed to extract similar fabrics from the database. It was confirmed that inferring the drape information and objective hand feeling of fabrics was to some extent possible by extracting similar fabrics from the database. In this study, the primary hand and total hand value(THV) of KES-FB system, which was constructed by Kawabata and other experiments, were used to quantify the objective hand feeling, because they are the most widely used. However, these standards can be changed over time; in order to be applied within the clothing industry, these standards may have to be changed to some extent. Moreover, it is notable that although objective hand feeling cannot be expressed in the 3D virtual costume program, it can be easily derived from the constructed database. Additionally, it is expected that the existing 3D virtual costume program will express the costumes more realistically by improving these results.

Comprehensive Evaluation System for Post-Metabolic Activity of Potential Thyroid-Disrupting Chemicals

  • Yurim Jang;Ji Hyun Moon;Byung Kwan Jeon;Ho Jin Park;Hong Jin Lee;Do Yup Lee
    • Journal of Microbiology and Biotechnology
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    • v.33 no.10
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    • pp.1351-1360
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    • 2023
  • Endocrine-disrupting chemicals (EDCs) are compounds that disturb hormonal homeostasis by binding to receptors. EDCs are metabolized through hepatic enzymes, causing altered transcriptional activities of hormone receptors, and thus necessitating the exploration of the potential endocrine-disrupting activities of EDC-derived metabolites. Accordingly, we have developed an integrative workflow for evaluating the post-metabolic activity of potential hazardous compounds. The system facilitates the identification of metabolites that exert hormonal disruption through the integrative application of an MS/MS similarity network and predictive biotransformation based on known hepatic enzymatic reactions. As proof-of-concept, the transcriptional activities of 13 chemicals were evaluated by applying the in vitro metabolic module (S9 fraction). Identified among the tested chemicals were three thyroid hormone receptor (THR) agonistic compounds that showed increased transcriptional activities after phase I+II reactions (T3, 309.1 ± 17.3%; DITPA, 30.7 ± 1.8%; GC-1, 160.6 ± 8.6% to the corresponding parents). The metabolic profiles of these three compounds showed common biotransformation patterns, particularly in the phase II reactions (glucuronide conjugation, sulfation, GSH conjugation, and amino acid conjugation). Data-dependent exploration based on molecular network analysis of T3 profiles revealed that lipids and lipid-like molecules were the most enriched biotransformants. The subsequent subnetwork analysis proposed 14 additional features, including T4 in addition to 9 metabolized compounds that were annotated by prediction system based on possible hepatic enzymatic reaction. The other 10 THR agonistic negative compounds showed unique biotransformation patterns according to structural commonality, which corresponded to previous in vivo studies. Our evaluation system demonstrated highly predictive and accurate performance in determining the potential thyroid-disrupting activity of EDC-derived metabolites and for proposing novel biotransformants.

A Study on Generation and Types of Mandelbrot Fractal Images (만델브로 프랙탈 이미지의 생성 및 형태 연구)

  • Lim, Mi-Jeong;Cho, Hyong-Je
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.1
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    • pp.217-222
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    • 2015
  • As a Creative Director one is always looking forward to formative elements of new design. The fractal image that is generated by a computer program instead of by hand suggests a geometric pattern that can grafted into a new design for each field. In this paper we look for information about the creation of a Mandelbrot fractal image that is being utilized in the design of various sectors like textile design, architectural design, exhibition design from pure painting by convergence of both technology and art composites. And it analyses about forms based on the formative principle of creation images.

Syntaxonomical and Synchorological Characteristics of the Cool-temperate Mixed Forest in the Southern Sikhote Alin, Russian Far East (극동러시아 남연해주의 냉온대 침활혼합림의 군락분류 및 분포적 특성)

  • Kim, Jong-Won;I. Manyko
    • The Korean Journal of Ecology
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    • v.17 no.3
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    • pp.391-413
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    • 1994
  • The northernmost type of the monogolian oak forests in the Russian Far East was studied in terma of syntaxonomy and synchorology. Hand-sorting method, computer program TWIN-SPAN, CANOCO and SYN-TAX III were engaged for the classification and data analysis. Correlation between plant communitie and environmental factors was analyzed by DCA (Detrended Correspondence Analysis) using NeCD (Net Contribution Degree) of species. Three plant communities were recognized: the Abieti holophyllae-Quercetum mongolicae, the Lespedezo-quercetum mongolicas and the Rosa ussuriensis-Quercus mongolica communit. They were included into the alliance Jeffersonio-Quercion monogolicae which is representative to the cool-Temperate forests mixed by conierous and broadleaved trees in southern Sikhote Alin. Human impact was signified as the most important factor to interpret the disjunction of plant communities of which DCA ordination yielded sharp contrasting objects (i.e. stands and species). In the amhi-Tonghae region, alaogous vegetation types such as the Jeffersonio-Quercion monogolicae of Russian Far East, the Quercion grosseserratae of Japanese Hokkaido and the Pino koraiensis-Quercion monogolicae of Koream peninusula were reviewed in terms of similarity of species composition and stratum structure. It is signified that the Jeffersonio-Quercion monogolicae was one of the continental types more similar to Korean alliance that to Japanese one.

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Backbone 1H, 15N, and 13C Resonance Assignments and Secondary-Structure of the Conserved Hypothetical Protein HP0892 of Helicobacter pylori

  • Han, Kyung-Doo;Park, Sung-Jean;Jang, Sun-Bok;Lee, Bong-Jin
    • Molecules and Cells
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    • v.25 no.1
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    • pp.138-141
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    • 2008
  • HP0892 (SwissProt/TrEMBL ID O25552) is a 90-residue conserved hypothetical protein from Helicobacter pylori strain 26695, with a calculated pI of 9.38 and a molecular mass of 10.41 kDa. It belongs to the Plasmid stabilization system protein family (PF05016) in the Pfam database. Proteins with sequence similarity to HP0892 exist in Vibrio choierae, Enterococcus faecalis, Campylobacter jejuni, Streptococcus pneumoniae, Haemophilus influenzae, Escherichia coli O157. Here we report the sequence-specific backbone resonance assignments of HP0892 using multidimentional heteronuclear NMR spectroscopy. About 97.0% (422/435) of the HN, N, CO, $C{\beta}$, $C{\alpha}$ resonances of 90 residues of HP0892 were assigned. On the basis of the resonance assignments, three helical regions and four strand regions were identified using the CSI program. This study is a prerequisite for calculating the solution structure of HP0892, and will be useful for studying its interaction with other molecules.

The Effect of Fashion Product Consumers' Need for Uniqueness on their Criteria of Clothing Selection and Switching Intentions

  • Park, Soojin;Park, Sookyeong
    • Journal of Fashion Business
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    • v.20 no.3
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    • pp.54-67
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    • 2016
  • The purpose of this study was to investigate the The effect of fashion consumer's need for uniqueness in their clothing purchase behavior and switching intention. A total of 332 cases were included as the subjects of this study and a survey method was used. The data were analyzed by factor analysis, reliability analysis, t-test and multiple regression analysis using the SPSS 22.0 statistical program. The results were as follows. 1. The 3 dimensions (creative choice, unpopular choice, and avoidance of similarity) were extracted from the consumer's need for uniqueness and consumer divided high uniqueness group, middle uniqueness group, low uniqueness group. 2. The 3 factor (esthetic, functional, practical) were extracted from the clothing selection criteria. The three group had a significant difference in clothing selection and switching intention. Aesthetic factor and practical factor were the highest in high uniqueness group. 3. The need for uniqueness has influence on the aesthetic factor and practical factor. The creative choice factor had positive effect on the aesthetic and practical factor, yet unpopular choice factor had negative effect on the practical factor. The need for uniqueness and aesthetic factor has positive influence on brand switching intentions. 4. There was difference in unpopular choices factor by gender. A monthly average expenditure on fashion products had a significant difference in three kind of need for uniqueness. Results of this study provide a basis understand need for uniqueness and their effect on consumer behavior.

CoMFA and CoMSIA Study on Angiotensin-Converting Enzyme (ACE) Inhibitors: a Molecular Design of Potential Hypertensive Drugs

  • San Juan, Amor A.;Cho, Seung-Joo
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2005.09a
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    • pp.249-255
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    • 2005
  • Angiotensin-converting enzyme (ACE) is primarily responsible for human hypertension. Current ACE drugs show serious cough and angiodema health problems due to the un-specific activity of the drug to ACE protein. The availability of ACE crystal structure (1UZF) provided the plausible biological orientation of inhibitors to ACE active site (C-domain). Three-dimensional quantitative structure-activity relationship (3D-QSAR) models have been constructed using the comparative molecula. field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) for a series of 28 ACE inhibitors. Alignment for CoMFA obtained by docking ligands to 1UZF protein using FlexX program showed better statistical model as compared to superposition of corresponding atoms. The statistical parameters indicate reasonable models for both CoMFA (q$^2$ = 0.530, r$^2$ = 0.998) and CoMSIA (q$^2$= 0.518, r$^2$ = 0.990). The 3D-QSAR analyses provide valuable information for the design of ACE inhibitors with potent activity towards C-domain of ACE. The group substitutions involving the phenyl ring and carbon chain at the propionyl and sulfonyl moieties of captopril are essential for specific activity to ACE.

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Imbedded Type Real-Time Fault Diagnosis for BLDC Motors (임베디드 타입의 실시간 BLDC 전동기 고장진단 시스템 구현)

  • Park, Jin-Il;Kim, Yong-Min;Lee, Dae-Jong;Cho, Jae-Hoon;Chun, Myung-Geun
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.23 no.4
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    • pp.62-71
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    • 2009
  • In this paper, we propose a fault diagnosis algorithm for BLDC motors by principle component analysis (PCA) and implement a real-time fault diagnosis system for BLDC motors. To verify the proposed diagnosis algorithm, various faulty data are acquired by Lab VIEW program from experimental system. We extract a fault feature using principle component analysis after preprocessing and then finally the fault diagnosis is performed by Euclidean similarity. Also, we embed the PCA algorithm and k-NN classification algorithm into a digital signal processor. From various experiments, we found that the proposed algorithm can be used as a powerful technique to classify the several fault signals acquired from BLDC motors.