• The Plant Pathology Journal
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• v.35 no.1
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• The Journal of the Korea Contents Association
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• v.19 no.5
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• pp.82-93
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• 2019

Fashion cannot encourage co-consumption with other people as a personal item, but it can lead to new consumer needs if fashion sharing service can professionally replace the time and cost of purchasing and managing goods. The purpose of this study is to empirically investigate the factors influencing the acceptance of fashion-sharing services based on the integration theory of user acceptance and utilization (UTAUT), and to discuss the virtuous cycle and sustainability pursuit of resources through the activation of the sharing. In this study, the research model for the acceptance of fashion sharing applications is schematized, and the survey was conducted 300 women aged 20~49 years. The screens of 'Project Anne', a representative fashion sharing service in Korea, were provided as a visual data. Reliability analysis, correlation analysis, confirmatory factor analysis, structural equation analysis, and multiple group analysis were performed using SPSS 23.0 and AMOS 22.0 statistical package for statistical analysis. As a result, efficiency and social influence positively influenced behavioral intention to use, and age has found that efficiency and social influences modulate the intensity of behavioral intention to use. Therefore, for the consumer acceptance and activation of fashion sharing services, marketing activities emphasizing efficiency and strengthening social influence factors are essential. Also, it is necessary to maintain the existing target group, 30~40s, and also construct additional products and price services for the 20s. This study is of academic significance in presenting basic data for empirical research on consumer acceptance of fashion sharing, and suggests a study on the influence relationship among user experience components for real users in the future.

• Journal of radiological science and technology
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• v.44 no.4
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• pp.359-365
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• 2021

A heavy particle accelerator is a device that accelerates particles using high energy and is used in various fields such as medical and industrial fields as well as research. However, secondary neutrons and particle fragments are generated by the high-energy particle beam, and among them, the neutrons do not have an electric charge and directly interact with the nucleus to cause radiation of the material. Quantitative evaluation of the radioactive material produced in this way is necessary, but there are many difficulties in actual measurement during or after operation. Therefore, this study compared and evaluated the generated radioactive material in the concrete shield for protons and carbon ions of specific energy by using the simulation code FLUKA. For the evaluation of each energy of proton beam and carbon ion, the reliability of the source term was secured within 2% of the relative error with the data of the NASA Space Radiation Laboratory(NSRL), which is an internationally standardized data. In the evaluation, carbon ions exhibited higher neutron flux than protons. Afterwards, in the evaluation of radioactive materials under actual operating conditions for disposal, a large amount of short-lived beta-decay nuclides occurred immediately after the operation was terminated, and in the case of protons with a high beam speed, more radioactive products were generated than carbon ions. At this time, radionuclides of ⁴⁴Sc, ³H and ²²Na were observed at a high rate. In addition, as the cooling time elapsed, the ratio of long-lived nuclides increased. For nonparticulate radionuclides, ³H, ²²Na, and for particulate radionuclides, ⁴⁴Ti, ⁵⁵Fe, ⁶⁰Co, ¹⁵²Eu, and ¹⁵⁴Eu nuclides showed a high ratio. In this study, it is judged that it is possible to use the particle accelerator as basic data for facility maintenance, repair and dismantling through the prediction of radioactive materials in concrete according to the cooling time after operation and termination of operation.

• Journal of Digital Convergence
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• v.19 no.7
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• pp.147-154
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• 2021

The pandemic age came to us after the COVID19 broke out in the early of 2020, which resulted in many changes in our everyday life. One of the changes is non-contact transactions in economic activities, especially contact-free financial transactions are ever-increasing activities in the pandemic age. In non-contact financial transactions, security and trust are important issues that users are concerned about the most. So the paper examines how security and trust have effects on the attitudes of bank customers who use mobile banking applications on their smartphones. The study conducted factor ananlysis to extract research constructs using survey data gathered during Nobember of 2020 through February of 2021 across the nation. After extracting the constructs, the study checked validity, sampling adequacy, and internal reliability with KMO, Bartlett's test, and Crombach Alpha respectively. To make sure the purpose of the study, I do regression analysis. All factors of attitude had impacts on willingness on both of transactions and purchasing financial products. The results could give guidelines banks which want to attract more customers in open-banking environment through ever-more secure and trusty mobile banking application.

• Journal of Food Hygiene and Safety
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• v.36 no.1
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• pp.34-41
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• 2021

The purpose of this research is to improve analysis methods of determining the contents of fatty acids in infant formulas and follow-up formulas. A gas chromatography (GC) method was performed on a GC system coupled to flame ionization detector, with a fused silica capillary column (SP2560, 100 m×0.25 mm, 0.20 ㎛). The method was validated using standard reference material (SRM, NIST 1849a). Performance parameters for method validation such as specificity, linearity, limits of detection (LOD) and quantification (LOQ), accuracy and precision were examined. The linearity of standard solution with correlation coefficient was higher than 0.999 in the range of 0.1-5 mg/mL. The LOD and LOQ were 0.01-0.06 mg/mL and 0.03-0.2 mg/mL, respectively. The recovery using standard reference material was confirmed and the precision was found to be between 0.8% and 2.9% relative standard deviation (RSD). Optimized methods were applied in sample analysis to verify the reliability. All the tested products had acceptable contents of fatty acids compared with component specification for nutrition labeling. The result of this research will provide efficient experimental information and strengthen the management of nutrients in infant formula and follow-up formula.

• Journal of Applied Biological Chemistry
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• v.64 no.1
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• pp.25-32
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• 2021

Phytochemicals include plant-derived natural products that promote and improve the human metabolism and physiological activity, and there is a lot of research to find the value of the molecules is in progress. Likewise, we obtained 288 fractions of Capsicum annuum L. extract in less than 20 h using HPLC/SPE/HPLC coupling experiment through Sepbox system, an effective separation system to search for active substances in natural resources and ensure efficacy and reliability. Therefore, this experiment allowed rapid identification of biologically active molecules from the extract compared to traditional separation processes. Of the above fractions, eight fractions showed the α-glucosidase inhibitory (AGI) activity and subsequent LC-MS analysis revealed one of the active molecules as luteolin 7-O-glucoside. In addition, we proved the increase in AGI activity according to deglycosylation of flavonoid glycoside. Therefore, this study suggests that the Sepbox system can quickly separate and identify active components from plant extract, and is an effective technique for finding new active substances.

• Korean Journal of Remote Sensing
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• v.38 no.6_4
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• pp.1935-1943
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• 2022

The development and application of a high-resolution soil moisture mapping method using satellite imagery has been considered one of the major research themes in remote sensing. In this study, soil moisture mapping in the test area of Jeju Island was performed. The soil moisture was calculated with optical images using linearly adjusted Synthetic Aperture Radar (SAR) polarization images and incident angle. SAR Backscatter data, Analysis Ready Data (ARD) provided by Google Earth Engine (GEE), was used. In the soil moisture processing process, the optical image was applied to normalized difference vegetation index (NDVI) by surface reflectance of KOMPSAT-3 satellite images and the land cover map of Environmental Systems Research Institute (ESRI). When the SAR image and the optical images are fused, the reliability of the soil moisture product can be improved. To validate the soil moisture mapping product, a comparative analysis was conducted with normalized difference water index (NDWI) products by the KOMPSAT-3 image and those of the Landsat-8 satellite. As a result, it was shown that the soil moisture map and NDWI of the study area were slightly negative correlated, whereas NDWI using the KOMPSAT-3 images and the Landsat-8 satellite showed a highly correlated trend. Finally, it will be possible to produce precise soil moisture using KOMPSAT optical images and KOMPSAT SAR images without other external remotely sensed images, if the soil moisture calculation algorithm used in this study is further developed for the KOMPSAT-5 image.

• Korean Journal of Optics and Photonics
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• v.33 no.5
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• pp.218-229
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• 2022

We have investigated reliable inspection methods for finding the defects generated during the manufacturing process of lightweight, large-aperture satellite telescope mirrors using silicon carbide, and we have measured the basic physical properties of the mirrors. We applied the advanced ceramic material (ACM) method, a combined method using liquid-silicon penetration sintering and chemical vapor deposition for the carbon molded body, to manufacture four SiC mirrors of different sizes and shapes. We have provided the defect standards for the reflectors systematically by classifying the defects according to the size and shape of the mirrors, and have suggested effective nondestructive methods for mirror surface inspection and internal defect detection. In addition, we have analyzed the measurements of 14 physical parameters (including density, modulus of elasticity, specific heat, and heat-transfer coefficient) that are required to design the mirrors and to predict the mechanical and thermal stability of the final products. In particular, we have studied the detailed measurement methods and results for the elastic modulus, thermal expansion coefficient, and flexural strength to improve the reliability of mechanical property tests.

• The Korean Journal of Franchise Management
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• v.13 no.2
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• pp.17-31
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• 2022

Purpose: With the development of the Internet, consumers can quickly access the electronic word-of-mouth. Consumers seek to reduce uncertainty by referring to the opinions of other consumers about products and services when making purchase decisions. In the food service industry, evaluating a restaurant before an actual visitation is difficult. Therefore, electronic word-of-mouth is important to interact with the customer in restaurants. as it can be used as an exchange of information in which consumers participate and interact with other customers. This study was conducted to verify how online word-of-mouth characteristics (Consensus, Vividness, Neutrality) on attitudes and visit intention from the perspective of social exchange theory. And it was performed to verify the structural relationship between short-term visit intention, mid-term visit and long-term visit intention. Research design, data, and methodology: A survey was conducted on customers who have visited restaurants. Of a total of 312 responses, 306 responses were used, excluding insincere responses and missing values for factors analysis. SPSS 25.0 and AMOS 25.0 were used for statistical analysis, and hypothesis testing was conducted after verifying the validity and reliability of the questionnaire items. Result: The result of the analysis showed that, consensus and neutrality have a positive effect on attitude but not much on vividness. In addition, consensus, vividness, and neutrality have no effect on the short-term visit intention. Finally, the short-term visit intention has a positive effect on mid-term visit intention, and mid-term visit intention has a positive effect on long-term visit intention. Conclusions: Based on the results, this study suggested that it is necessary to have practical implications for marketing and monitoring restaurant reviews in consideration of the characteristics of electronic word-of-mouth. When managing electronic-word-of-mouth, it is necessary to manage the consensus and neutrality is essential to provide sufficient information about the restaurant. The focus should not only be on vividness, such as photos and videos. In addition, restaurants should also provide a good experience for first-time visitors as the short-term visit intention positively affects mid-term and long-term visit intention.

• The Korean Journal of Franchise Management
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• v.13 no.3
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• pp.1-15
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• 2022

Purpose: In the restaurant sector, it has been known that consumers' positive perception of brands influences their positive WOM intention, and information sources play an important role in increasing credibility by enhancing consumer awareness and developing differentiated brands. This study examines the effects of information sources (e.g., advertisement, WOM, SNS) on trust (cognitive and affective) and, WOM and eWOM intention in the restaurant context. In the model, cognitive and affective trust play mediating roles in the relationships between information sources (e.g., advertisement, WOM, SNS) WOM and eWOM intention. Research design, data, and methodology: Research models and hypotheses were developed according to the research direction. The survey questionnaire items were developed and used appropriately according to the contents of this paper based on prior studies. All constructs were measured with multiple items developed and validated in prior studies. A total of 502 responses were collected from an online survey. The research model was evaluated using SmartPLS 4.0. Frequency analysis was performed to understand the demographic characteristics of the survey respondents. The reliability, convergent validity, and discriminant validity were assessed using measurement model analysis. The proposed model was verified using the structural equation model. Results: Advertisement, WOM, and SNS information sources all had a positive effect on affective trust, whereas only WOM had a significant effect on cognitive trust. In addition, affective trust had a positive effect on cognitive trust and eWOM intention but did not affect WOM intention. Finally, cognitive trust was found to have a positive effect on both WOM intention and eWOM intention. Conclusions: This study redefines the concept of where restaurant service companies should focus when providing consumers with information about their products and services. As a result, the conceptual framework of positive word of mouth intention to increase new customer visits to the restaurant brand has been expanded. In addition, this study not only presents an information source management strategy for restaurant brands, but also presents practical implications for resource allocation guidelines for customer management in the restaurant sector.