• BMB Reports
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• v.46 no.3
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• pp.157-162
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• 2013

Human ${\alpha}$-galactosidase A (GLA) has been used in enzyme replacement therapy for patients with Fabry disease. We expressed recombinant GLA from Chinese hamster ovary cells with very high productivity. When compared to an approved GLA (agalsidase beta), its size and charge were found to be smaller and more neutral. These differences resulted from the lack of terminal sialic acids playing essential roles in the serum half-life and proper tissue targeting. Because a simple sialylation reaction was not enough to increase the sialic acid content, a combined reaction using galactosyltransferase, sialyltransferase, and their sugar substrates at the same time was developed and optimized to reduce the incubation time. The product generated by this reaction had nearly the same size, isoelectric points, and sialic acid content as agalsidase beta. Furthermore, it had better in vivo efficacy to degrade the accumulated globotriaosylceramide in target organs of Fabry mice compared to an unmodified version.

• Journal of Navigation and Port Research
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• v.41 no.3
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• pp.137-148
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• 2017

Port service level is a metric of competitiveness among ports for the operating/managing bodies such as the terminal operation company (TOC), Port Authority, or the government, and is used as an important indicator for shipping companies and freight haulers when selecting a port. Considering the importance of metrics, we developed software to objectively define and manage six important service indicators exclusive to container and bulk terminals including: berth occupancy rate, ship's waiting ratio, berth throughput, number of berths, average number of vessels waiting, and average waiting time. We computed the six service indicators utilizing berth 1 through berth 5 in the container terminals and berth 1 through berth 4 in the bulk terminals. The software model allows easy computation of expected ship's waiting ratio over berth occupancy rate, berth throughput, counts of berth, average number of vessels waiting and average waiting time. Further, the software allows prediction of yearly throughput by utilizing a ship's waiting ratio and other productivity indicators and making calculations based on arrival patterns of ship traffic. As a result, a TOC is able to make strategic decisions on the trade-offs in the optimal operating level of the facility with better predictors of the service factors (ship's waiting ratio) and productivity factors (yearly throughput). Successful implementation of the software would attract more shipping companies and shippers and maximize TOC profits.

• Journal of Navigation and Port Research
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• v.32 no.8
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• pp.621-628
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• 2008

Container terminals keenly compete with one another because of the continuous increase of container flows and the appearance of large-sized vessels. Major cargo handling equipment manufacturers are interested in the development of new conceptual equipment to greatly increase its productivity. In this study, a two-phase procedure is suggested for selecting the optimal development alternatives, when various development alternatives for container cranes are given. The first phase removes the alternatives that violate essential requirements and the second phase selects final alternatives by the evaluation of experts and the linear assignment method. Finally, a case applying the procedure is provided.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.339-344
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• 2006

Container terminals compete with other ones because of the continuous increase of container flows and the appearance of large-sized vessels. Major port equipment manufacturers are interested in the development of new conceptual equipment ot greatly increase the productivity. In this study, a two-phase procedure is suggested for selecting the optimal development alternatives, when there are various development alternatives for container cranes, typical equipment in ports. The first phase removes the alternatives that violate essential requirements and the second phase selects find alternatives by using AHP (Analytic Hierarchy Process). Finally and example applying the procedure is provided.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.156-156
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• 2023

Salinity is a major abiotic stress that limits rice productivity in many regions of the world. In order to develop salt stress tolerant rice plants, genetic engineering is a promising approach. We characterized the molecular function of rice C3H2C3 as a really interesting new gene (RING). Oryza sativa RING finger protein H2-3 (OsRFPH2-3) was highly expressed in 100 mM NaCl. To identify the localization of OsRFPH2-3, we fused vectors that include C-terminal GFP protein (35S;;OsRFPH2-3-GFP). OsRFPH2-3 was expressed in the nucleus in rice protoplasts. An in vitro ubiquitin assay demonstrated that OsRFPH2-3 possessed E3-ubiquitin ligase activity. However, the mutated OsRFPH2-3 were not possessed any E3-ubiquitin ligase activity. Under salinity conditions, OsRFPH2-3-overexpressing plants exhibited higher chlorophyll, proline, SOD, POD, CAT, and soluble sugar contents and lower H₂O₂ accumulation than wild-type plants, supporting transgenic plants with enhanced salinity tolerance phenotypes. OsRFPH2-3-overexpressing plants exhibited low Na+ accumulation and Na+/K+ ratios in their roots. Theses results suggest that overexpression of OsRFPH2-3 can make plant insensitivity about salinity conditions.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.189-189
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• 2022

Soil salinity negatively affects plant growth, productivity, and metabolism. Rice is known to have more sensitive phenotypes than other cereal crops, such as wheat, sorghum, and barley. We characterized the molecular function of rice C3HC4 as a really interesting new gene (RING). Oryza sativa RING finger protein H2-3 (OsRFPH2-3) was highly expressed in 100 mM NaCl. To identify the localization of OsRFPH2-3, we fused vectors that include C-terminal GFP protein (35S;;OsRFPH2-3-GFP). OsRFPH2-3 was expressed in the nucleus in rice protoplasts. An in vitro ubiquitin assay demonstrated that OsRFPH2-3 possessed E3-ubiquitin ligase activity. However, the mutated OsRFPH2-3 were not possessed any E3-ubiquitin ligase activity. Under normal conditions, there is no significant phenotypic difference between transgenic plants and WT plants. However, OsRFPH2-3-overexpressing plants exhibited higher fresh weight and length under saline conditions. Also, transgenic plants maintain higher chlorophyll, proline, and soluble sugar contents and lower H₂O₂ and MDA contents than the wild type; these results support transgenic plants with enhanced salinity tolerance phenotypes.

• Journal of the Korea Society of Computer and Information
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• v.14 no.10
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• pp.55-61
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• 2009

This paper describes a point of production(POP) system which collects and manages real-time shop floor machining information in a wafer dicing process. The system are composed of POP terminal, line controller and network. In the configuration of the system, LAN and RS485 network are used for connection with the upper management system and down stratum respectively. As a bridge between POP terminal and server, a line controller is used. The real-time information which is the base of production management are collected from information resources such as machine, product and worker. The collected information are used for the calculation of optimal cutting condition. The collection of the information includes cutting speed, spout of pure water, accumulated count of cut in process for blade and wafer defect. In order to manage machining information in wafer dicing process, production planning information is delivered to the shop floor, and production result information is collected from the shop floor, delivered to the server and used for managing production plan. From the result of the system application, production progress status, work and non-working hour analysis for each machine, and wafer defect analysis are available, and they are used for quality and productivity improvements in wafer dicing process. A case study is implemented to evaluate the performance of the system.

• Journal of Digital Convergence
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• v.15 no.10
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• pp.31-43
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• 2017

This study was aimed at analyzing the operational efficiency of DPAs(the delivery and pickup agents), which serve as sub-terminals in parcel delivery services, using CCR/BCC models in DEA. The input variables included the number of employees, and the amount of equipment and the operating expenses. The output variable was revenue value. The efficiency for the period from 2014 to 2016 was analyzed. The results revealed that the operational efficiency improved in 2016, compared with 2014, in both CCR and BCC models. According to the benchmarking analysis, DMU 1 and DMU 7 showed higher efficiency in 2016. The inefficiency analysis based on the BCC model showed increased efficiency of all factors in 2016 when compared with 2014. The Malmquist productivity index (MPI) dropped slightly as a result of technical changes and indicated a declining technical efficiency in all DMUs. This study suggests the need for government-led systematic improvement and support for DPAs by providing current insight into the parcel delivery industry and analyzing DPAs' operational efficiency in Korea for the first time. This research performed efficiency analysis of DPAs located in new town of paju and gimpo cities. In future research, comparative study on efficiency analysis including new town, old town area, and other cities are needed.

• Journal of Navigation and Port Research
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• v.42 no.1
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• pp.47-56
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• 2018

In this study, the focus is on the issue of whether a container terminal is facing the limitation of its productivity for serving mega-vessels with numerous containers. In order to enhance the terminal operations, a new conceptual design of the container handling system have been proposed. This research focuses on the rail-based container transport system and its operations. This system consists of rail-based shuttle cranes and rail-based transporters called flatcars. The deadlock problem for managing automated transporters in container terminals has been an important issue for a long measurement of time. Therefore, this study defines the deadlock situation and proposes its avoidance rules at the rail-based container transport system, which is required to handle numerous container throughput operations. The deadlock in the rail-based container transport system is classified into two parts: deadlock between cranes and flatcars; deadlock between flatcars. We developed the simulation model for use with characterizing and analyzing the rail-based container transport system. By running the simulation, we derived possible deadlock situations, and propose the several deadlock avoidance algorithms to provide results for these identified situations. In the simulation experiments, the performances of the deadlock avoidance algorithms are compared according to the frequency of deadlocks as noted in the simulations.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.21 no.6
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• pp.119-132
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• 2011

Mobile devices such as smart phones have brought big changes to be called as 'mobile big bang' against which we can't go. Mobile banking service and MTS(Mobile Trading System) are freely available at any time, anywhere and we are able to activate communications between financial company staffs out of the office and take care of business works even remotely by using mobile devices. Mobile devices are approaching as 'smart mobile innovation' to improve an enterprise productivity and competitiveness, but threats which engaged in unfair trading behaviors or unwholesome business works in finance companies are increasing and the customer's information can be leaked out by using the nonpublic official information and mobile devices. Therefore, we have to analyze the potential problems and take the necessary countermeasures with preemptive steps to protect the customer's information and improve the financial trading soundness and fairness. In this paper, we would like to suggest countermeasures and threats against using the financial company's mobile devices focusing on the financial investment companies by 'Capital Market and Financial Investment Business Act'.