• Title/Summary/Keyword: producing

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PRIME-PRODUCING POLYNOMIALS RELATED TO CLASS NUMBER ONE PROBLEM OF NUMBER FIELDS

  • Jun Ho Lee
    • Bulletin of the Korean Mathematical Society
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    • v.60 no.2
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    • pp.315-323
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    • 2023
  • First, we recall the results for prime-producing polynomials related to class number one problem of quadratic fields. Next, we give the relation between prime-producing cubic polynomials and class number one problem of the simplest cubic fields and then present the conjecture for the relations. Finally, we numerically compare the ratios producing prime values for several polynomials in some interval.

Detection of VTe-producing E coli using PCR method (PCR 기법 이용 VTe 분비 대장균 검출)

  • Yoon, Soon-seek;Park, Nam-yong;Lim, Jeong-taek
    • Korean Journal of Veterinary Research
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    • v.36 no.3
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    • pp.607-614
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    • 1996
  • Several methods for rapid and accurate detection of VTe-producing E coli were established. These methods contain beta-glucuronidase-secretion test, beta-haemolysis-production test in blood agar, verocytotoxicity test, and PCR. All of the VTe-producing strains made beta-haemolysis on 5% sheep blood agar. VTe-producing strains secreted beta-glucuronidase whereas 0157:H7 strains producing VTI or VTII did not secrete that enzyme. Verocytotoxicity test was established for rapid diagnosis. VTe detection was rapider in Vero cell suspension than Vero cell monolayer. In PCR, there was a positive result only in VTe-producing E coli, not in VTI or VTII-producing E coli. In this experiment, 165 strains of E coli were islated from feces or intestinal contents of post-weaning piglets showing nervous sign or diarrhea. And 20 strains of E coli that produced VTe were selected by verocytotoxicity test and PCR. According to these experiments, there was a direct correlation between verocytotoxicity test and PCR. And verocytotoxicity test is recommended as a routine diagnostic method and PCR does as a accurate diagnostic method to detect VTe-producing E coli.

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Comparative in vitro and in vivo Antibacterial Activities of Cefatrizine/clavulanic Acid Combination and Other $\beta$-lactam Antibiotics (Cefatrizine과 clavulanic acid 병합제의 in vitro 및 in vivo 항균력)

  • 최성학;김지영;김계원;김원배;심미자
    • Biomolecules & Therapeutics
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    • v.7 no.1
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    • pp.44-53
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    • 1999
  • The resistant strains due to the extended-spectrum $\beta$-lactamase (ESBL) were susceptible to cefatrizine combined with clavulanic acid. The purpose of this study was to evaluate the in vitro and in vivo antibacterial activities of cefatrizine/clavulanic acid (CTRZ/CV) combination at a ratio of 2 : 1 in comparison with cefaclor (CCLO), cefuroxime (CRXM), cefuroxime axetil (CRXMA) and amoxicillin/clavulanic acid (AMXCCV). CTRZ/CV showed good activity against laboratory strains of gram-positive and gram-negative bacteria and exhibited excellent antibacterial activity against $\beta$-lactamase-producing strains. The bactericidal activity of CTRZ/CV was superior to that of CCLO and CRXM, and almost equal to that of AMXCCV against the $\beta$-lactamase-producing strains. The in vitro results were substantiated. by in vivo mouse experimental infection studies with $\beta$-lactamase-producing and non-producing strains. In mixed experimental infection due to $\beta$-lactamase-producing and non-producing strains, the therapeutic efficacy of CTRZ/CV was superior to that of CTRZ, CCLO, CRXMA and AMXCCV. In respiratory tract infection in mice due to Klebsiella pneumoniae EB4O, CTRZ/CV was more erective than CCLO, CRXMA and AMXCCV and also more efficacious than CCLO, CRXMA and AMXCCV in urinary tract infection in mice due to Escherichia coli EB13. These results indicate that CTRZ/CV is a useful drug for the treatment of infection caused by $\beta$-1actamase-producing strains including ESBL-producing strains.

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The Effective Isolation of a Mosquitocidal Bacteria, Bacillus thuringiensis Subsp. israelensis (모기 살충성 세균 B. thuringiensis subsp. israelensis의 효과적인 분리 방법)

  • 김광현;이광배;신두만
    • Journal of environmental and Sanitary engineering
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    • v.13 no.2
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    • pp.34-39
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    • 1998
  • For more convenient and rapidly isolation of Bacillus thuringiensis subsp. israelensis(Bti), 1) heat treatment spore forming bacteria, 2) growth in enrichment culture media for Bacillus sp. and 3) selection of bacteria producing a lecithinase for Bacillus thuringiensis subsp. israelensis, were performed. Spore forming bacteria were counted 4.8 $\times $ 10$^{8}$cells/g soil on NAPGCY media, 9.2 $\times $ 10$^{7}$cells/g soil on NA media, and 3.6 $\times $ 10$^{8}$cells/g soil on NAAC media, respectively. Bacteria producing only a lecithinase were reached at 25.2% on medium contained egg york, bacteria only producing a delta-endotoxin were reached at 23.2% by phase contrast microscope, and bacteria producing a lecithinase & a delta-endotoxin simultaneously were reached at 13.7%. Bacillus thuringiensis which producing a lecithinase and a delta-endotoxin simultaneously among bacteria producing a lecithinase, were reached at 56.5%; A half of Bacillus thuringiensis was produced a delta-endotoxin, but not produced a lecithinase. Among 8 isolates of Bacillus thuringiensis, two strain of Bti which has a mosquito-cidal toxin, were detected by PCR using a specific primer of $\delta $-endotoxin gene from Bti.

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Expression of E. coli LacZ Gene in Bovine Morular or Blastocysts after Microinjection of Retrovirus Vector-Producing Cells into the Perivitelline Space of One-to Four-Cell Embryos (체외생산된 우유정란으로부터 형질전환우의 생산성 제고를 위한 Retrovirus Vector System의 이용성 검토)

  • 김태완;박세필
    • Korean Journal of Animal Reproduction
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    • v.19 no.1
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    • pp.35-41
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    • 1995
  • In this study, we have tested whether the retrovirus vector system is applicable in transgenic cattle production. To overcome low infectivity of currently available retrovirus vector system we have directly microinjected retrovirus-producing cells into the perivitelline space of the day 1.5 embryos. The virus-producing cell line was designed to release replication-defective retrovirus encapsidated with Gibbon ape leukemia virus (GaLV) envelope protein. E. coli LacZ gene was used as a marker gene to facilitate evaluation of the transgene expression and X-gal staining at morula or blastocyst stage resulted in expression of E. coli LacZ gene The results in these experiments were summarized as follows : 1. The lowest concentration of polybrene necessary for efficient virus infection was Sf' g/ml. 2. Development rate from day 1.5 embryos microinjected with virus-producing cells to the morulae /blastocysts was 29%. 3. 21% of the morulae /blastocysts were LacZ+. 4. There was no evidence that the retrovirus-producing cells used in this study produced replication-competent retrovirus.

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On Production System by One-Person Production System in Broadcasting Program Production (방송프로그램제작에 있어 1인 제작시스템 변화)

  • Lee, Jong-Tak;Kwak, Hoon-Sung
    • The Journal of the Korea Contents Association
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    • v.7 no.8
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    • pp.117-124
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    • 2007
  • The one-person producing system is that planning program, arrangements, composition, taking pictures and edition are all done by only one person. In the reality of the producing systems of the broadcasting programs, as the number of the broadcasting stations, which apply one-person producing systems, is gradually increasing, the study on the change is going on. In this study I grasped the merits and demerits of both systems ; multi men producing system and one-person producing system. And then I analyzed the sudden change of the method of the producing programs into one using the 6m digital camera. The merits of producing programs by means of the 6m digital cameras are following ; the mobility of producing programs, a close contact with the source, the enlargement of the diversity of the contents and cutting down expenses. The demerits of it are following ; a lowering of the degree of completion and the unstableness of an image. Nowadays because of the enlargement of the rate of the outsourcing production, the number of the independent production studios rapidly increased. From the viewpoint of cutting down expenses and the diversity of contents, the number of broadcasting programs produced by means of one-person producing broadcasting program system is increasing.

A Study of the Effects of Vowels on the Production of English Labials /p, b, f, v/ by Korean Learners of English (영어학습자의 순음 /p, b, f, v/ 발성에 미치는 모음의 영향 연구)

  • Koo, Hee-San
    • Phonetics and Speech Sciences
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    • v.2 no.3
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    • pp.23-27
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    • 2010
  • The purpose of this study was to find how English vowels /a, e, i, o, u/ affect the production of English labials /p, b, f, v/ by Korean learners of English. Sixty syllables were composed by five vowels and four labials in the syllable types CV, VC, and VCV. The nonsense syllables were produced three times by nine subjects. The major results show that (1) in inter-vocalic position, the subjects had higher scores in producing /v/ composed with /a, e, o/ and /u/, while subjects had lower scores in producing /p/ with /i/ and /o/, (2) in post-vocalic position, the subjects had higher scores in producing /v/ and /f/ with /a, e/, and /o/, while subjects had lower scores in producing /b/ with /e/ and /i/, and (3) in pre-vocalic position, the subjects had higher scores in producing /v/ with /e, o, u/ and /f/ with /u/, while subjects had lower scores in producing /b/ with /e/, /i/ and /u/. The results suggest that on the whole, Korean learners of English have much difficulty in producing /p/ with /i/ in inter-vocalic condition and /b/ with /i, /e/ in pre-vocalic position.

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Characterization of Tryptamine-Producing Bacteria Isolated from Commercial Salted and Fermented Sand Lance Ammodytes personatus Sauces (시판 까나리(Ammodytes personatus) 액젓에서 분리한 tryptamine 생성균의 특성)

  • Um, In-Seon;Kim, Tae-Ok;Kim, Hee-Dai;Park, Kwon-Sam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.6
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    • pp.792-799
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    • 2016
  • We isolated seven tryptamine-producing bacteria from commercial salted and fermented sand lance (Ammodytes personatus) sauces using an L-tryptophan decarboxylating medium. These tryptamine-producing bacteria, identified using an API kit and 16S rRNA analysis, included Lysinibacillus xylanilyticus (one strain), Lysinibacillus fusiformis (four strains), and Staphylococcus epidermidis (two strains). Lysinibacillus spp. produced the highest levels of tryptamine in culture broth containing 0.5% L-tryptophan, compared with 1.0% and 2.0% preparations. After 72 h of incubation, Staphylococcus epidermidis produced the highest levels of tryptamine ($60.50{\mu}g/mL$ and $664.86{\mu}g/mL$) in culture broth containing 2.0% L-tryptophan. While Lysinibacillus spp. comprised the dominant tryptamine-producing bacteria in sand lance sauces, Staphylococcus epidermidis also showed high tryptamine-producing activity. This is the first report on the isolation and identification of tryptamine-producing bacteria in sand lance sauces.

A Comparative Study on the Mushroom Populations between Matsutake-Producing and Non-producing Pinus densiflora Stands (송이발생림(松栮發生林)과 미발생림(未發生林)의 버섯분포(分布)에 관(關)한 비교연구(比較硏究))

  • Lee, Kyung Joon;Kim, Yang Sup;Lee, Tae Soo;Kim, Kyo Soo
    • Journal of Korean Society of Forest Science
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    • v.72 no.1
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    • pp.27-31
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    • 1986
  • To compare the fungal distribution of matsutake-producing (Tricholoma matsutake) stand with that of non-producing stand, mushrooms on forest floor were collected in September of 1984 from 28-year-old non-producing and 55-year-old matsutake-producing stands of Pinus densiflora in Kangwon Province. A total of 18 fungal species in 12 genera were collected from a non-producing stand, and all the species except one were putative ectomycorrhizal. Common genera in non-producing stand included Suillus, Russula, Lactarius, Hydnum, and Amanita. From a matsutake-producing stand a total of 30 fungal species in 22 genera were collected, of which 22 species were ectomycorrhizal. Common genera in the producing stand included Hydnum, Tricholoma, Laccaria, and Cantharellus. Common species in both stands were six species only. It is concluded that matsutake-producing stand had more diversified fungal population than non-producing stand, probably because of higher contents of organic matters in soil and better development of understory vegetation through the ageing of the stand.

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Virulence factors, antimicrobial resistance patterns, and genetic characteristics of hydrogen sulfide-producing Escherichia coli isolated from swine

  • Park, Hyun-Eui;Shin, Min-Kyoung;Park, Hong-Tae;Shin, Seung Won;Jung, Myunghwan;Im, Young Bin;Yoo, Han Sang
    • Korean Journal of Veterinary Research
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    • v.55 no.3
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    • pp.191-197
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    • 2015
  • Escherichia (E.) coli is commensal bacteria found in the intestine; however, some pathogenic strains cause diseases in animals and humans. Although E. coli does not typically produce hydrogen sulfide ($H_2S$), $H_2S$-producing strains of E. coli have been identified worldwide. The relationship between virulence and $H_2S$ production has not yet been determined. Therefore, characteristics of $H_2S$-producing isolates obtained from swine feces were evaluated including antibiotic resistance patterns, virulence gene expression, and genetic relatedness. Rates of antibiotic resistance of the $H_2S$-producing E. coli varied according to antibiotic. Only the EAST1 gene was detected as a virulence gene in five $H_2S$-producing E. coli strains. Genes conferring $H_2S$ production were not transmissible although the sseA gene encoding 3-mercaptopyruvate sulfurtransferase was detected in all $H_2S$-producing E. coli strains. Sequences of the sseA gene motif CGSVTA around Cys238 were also identical in all $H_2S$- producing E. coli strains. Diverse genetic relatedness among the isolates was observed by pulsed-field gel electrophoresis analysis. These results suggested that $H_2S$-producing E. coli strains were not derived from a specific clone and $H_2S$ production in E. coli is not associated with virulence genes.