• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1176-1184
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• 2013

Anti-hepatocyte growth factor (anti-HGF) monoclonal antibodies (mAbs) are potential therapeutics against various cancers. Screening for high-producer clones is a time-consuming and complex process and is a major hurdle in the development of therapeutic mAbs. Here, we describe an efficient approach that allows the selection of high-producer Chinese hamster ovary (CHO) cell lines producing the novel anti-HGF mAb SFN68, which was generated previously by immunizing HGF bound to its receptor c-Met. We selected an SFN68-producing parental cell line via transfection of the dihydrofolate reductase-deficient CHO cell line DG44, which was preadapted to serum-free suspension culture, with an SFN68-expression vector. Subsequent gene amplification via multiple passages of the parental cell line in a methotrexate-containing medium over 4 weeks, followed by clonal isolation, enabled us to isolate two cell lines, 2F7 and 2H4, with 3-fold higher specific productivity. We also screened 72 different media formulated with diverse feed and basal media to develop a suboptimized medium. In the established suboptimized medium, the highest anti-HGF mAb yields of the 2F7 and 2H4 clones were 842 and 861 mg/l, respectively, which were about 10.5-fold higher than that of the parental cell line in a non-optimized basal medium. The selected CHO cell lines secreting high titers of SFN68 would be useful for the production of sufficient amounts of antibodies for efficacy evaluation in preclinical and early clinical studies.

• The Journal of Fisheries Business Administration
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• v.26 no.1
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• pp.9-27
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• 1995

From now on, the direction of fishery products circulation measure should be improved the system to give just the right of free choice of sale route like retail, direct sale, direct dealings and direct shipping only for fishermen to produce them diversifing the function and the role of current fish market in the production to prepare the fishery internationalization, to improve the competitiveness of coastal and off-shore fishery. Practically wholesale market in land and fish market in the production have the different function and role, the organization of wholesale market in land can't substitute the essential function and role which fish market in the production should perform. So far, fisheries cooperatives as a managing subject of fish market in the production have depended on the consignment sales and purchases of fishery products but, from now on, it should be explore the widespread supply way of fishery products by producing value - added fishery products that should bring higher demand from the consumers, producer - consumer direct marketing system through not only consignment sales but also direct sales that common processing and manufacturing system of fishery products under the management of FC has been established. The direction of the idealest structural improvement of fishery production circulation organization is to establish the the position of the fishery retail price leader based on the standard of market in the fisheries production due to existing characteristics of fisheries, accordingly, to harmony the market organization of direct transportation, direct sales and direct dealings form by producer and group of producers with market organization in the fisheries production, and to make the condition to do the appropriate distributive function.

• Journal of the Korean earth science society
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• v.29 no.1
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• pp.13-28
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• 2008

About 450 specimens of Lockeia were discovered from the lacustrine siltstone of the Early Cretaceous Jinju Formation of Jin Island, southern coast of Korea. They are very elongated, seed-shaped Lockeia characterized by a large size, mostly 60-70 mm long and 15-20 mm wide. They are characteristically sharp longitudinal furrow bounded by steeply inclined both margins, elevated marginal rims and sharp pointed both longitudinal furrow ends. This trace fossil is herein described as Lockeia gigantus ichnosp. nov. Plicatounio, a freshwater bivalve which does not occur occurs occasionally within Lockeia gigantus is regarded as the most-likely producer of this resting trace fossil. This new trace fossil represents the largest Lockeia ever known and the first record of Lockeia from the Cretaceous non-marine deposits in the world. This fossil also represents an unusual example of resting trace fossil (Lockeia) associated with a possible producer (bivalve Plicatounio) lived in community in the shallow lacustrine environment.

• The Journal of Fisheries Business Administration
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• v.32 no.2
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• pp.73-89
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• 2001

The market conditions of sea mustard is changing by overproduction, decreasing price, Import of blanched and salted sea mustard from China into Korea domestic market and increasing market share of sea mustard of China in Japan. In addition, the price support program in sea mustard aquaculture must be modified due to the restriction of domestic support by international organization such as WTO. There are two ways to solve those problems. First is that finding a way to solve the overproduction of sea mustard. One of possible ways is the production adjustment by Marketing Order. Second is that finding an alternative way to replace price support program. The possible way is Direct Payment instead of purchase stockpile system. To introduce marketing Order, outlook center, organization of self-management, production adjustment through output control measure, improvement of market structure, and education/publicity arc necessary. Also, to implement marketing order, setting a model business by government is required. There are two steps for implementation of marketing order. First step is to construct Order Committee including organization of producer, people related marketing. However, this committee must run by government for certain short-term. Second step is to improve quality of product and acceleration of demand. At visual point that enforcement of the first step is completed, government has process that government transfers Order Committees self-correcting. It is desirable that government only conduct the support acts such as quality improvement and acceleration of demand. Also, at early stage it is necessary to have aid system for marketing order For example, we can expect that income increase by production adjustment in long run. However, in short run the income of producer may decrease so, it is required to compensate his economic lose. For compensation, The useful means that can be utilized is direct payment. Direct payment is not continued policy. Also, when production adjustment policy such as Marketing Order has effective results, Direct Payment as an assistant measure must be reduced or abolished. Therefore, when production adjustment acts as an effective tool to control overproduction, Direct Payment system.

• Fashion & Textile Research Journal
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• v.17 no.6
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• pp.907-918
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• 2015

With the pervasiveness of digital environment and expansion of new media, ICT (Information and Communication Technology) leads the role of consumer to change from a passive customer into an active co-producer. As customer participation in the fashion industry increases, this study attempts to explore the cases of customer participation as a co-producer in fashion industry through analyzing business models of companies, including Threadless, Blank label, Cut on Your Bias, Acustom Apparel, Thinkgivers. The results show that customer participation is mainly facilitated through design participation in fashion industry, with different level of participation and IT as well as different contribution target. Compared to the past, consumer’s preference is reflected through participation before the stage of production. Results indicate three main characteristics of customer participation in fashion industry. First, value co-creations using customer experiences and understanding customer needs are important. Second, active online communication among customers are critical. Third, it is important to ensure customer’s own item. From a customer perspective, participation enables them to purchase their own distinctive product and offers exciting experience and satisfaction over the production processes, and compensation can be used to increase customer participation. On the other hand, companies using customer participation can take advantage of cost reduction in product development, inventory, and marketing. Customer participation, as a win-win strategy for business and consumer, is expected to expand further in the future with the development of ICT.

• Journal of Mushroom
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• v.12 no.2
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• pp.82-87
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• 2014

Commonly known as the button mushroom, Agaricus bisporus is one of the most widely cultivated mushroom species of edible fungi. In the breeding of new button mushroom, Seolwon was developed by crossing two homokaryons. Because of the predominantly pseudohomothallic life cycle, only a small percentage of homokaryotic meiospores are produced, which do not fruit. Homokaryotic cultures derived from these types of single spores produce a vegetative mycelium that contain a variable number of genetically identical nuclei per cell. After crossing two homokaryons, hybrids were cultivated on a small scale and on a commercial scale at a farm. The spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of Seolwon on CDA was better at $25^{\circ}C$ when it was compared with that of Seolgang. The mature cap shape of new strain Seolwon is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 39.7 mm on average. In comparison with white strain Seolgang, the strain had a yield that was 11% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 9.7% firmer with a good shelf life. Days of fruiting body were 1-2 days later than those of Seolgang. The physical characteristics such as springiness, chewiness, adhesiveness, gumminess were better than that of Seolgang.

• Journal of the Economic Geographical Society of Korea
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• v.16 no.3
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• pp.344-358
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• 2013

Vietnam's growth as the second largest coffee producer in the world has been fueled by domestic migration into central highlands. However, the detail migration processes have not been explicitly discussed. In particular, the existing literatures tend to describe the migration flow in a framework of planned migration versus spontaneous migration, paying less attention to the linkage between them. With a case study of Dak Lak province which is the leading robusta producer in Vietnam, this research investigates the linkage between planned and spontaneous migration in a multiscalar approach. The statistic data allow the researcher to know temporal association between the two type of migration in a provine scale. The survey and interview about two coffee-producing communes - Ea H'Ding in Cu M'gar district and Doan Ket in Krung Buk district - inform us that the linkage between planned and spontaneous migration is contingent to the local contexts including ethnic composition, timing of settlements, governement's development program, and timing of a large flow of in-migration.

• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.59-65
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• 2005

Poly-${\gamma}$-glutamic acid (PGA) is an unusual anionic polypeptide and has great potential as an environmentally and industrially significant biodegradable material. A new ${\gamma}$-PGA producer, Bacillus mesentericus MJM1, with high production capacity was isolated from Korean domestic Chungkuckjang bean paste. It produced ${\gamma}$-PGA at the level of 10 g/l in suitable media. The viscosities of 5% initially extracted mucin and purified ${\gamma}$-PGA solutions were 660 cps and 600 cps, respectively. The produced ${\gamma}$-PGA polymer consisted of 2,000 glutamic acid residues with even proportion of L and D types with molecular mass of about 200- 300 kDa. Bacillus mesentericus MJM1 displayed ${\gamma}$-glutamyltranspeptidase (${\gamma}$-GTP) activity that is known to play a key role in ${\gamma}$-PGA biosynthesis. The ${\gamma}$-GTP coding region was located on the plasmid of 5.8 kb. The plasmid, named pMMH1, is a rolling-circle replication (RCR) plasmid and additionally contained a replication origin and type I signal peptidase (sipP) coding region.

• Journal of IKEEE
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• v.24 no.3
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• pp.791-796
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• 2020

In this paper, "Q-Bone", a 3^rd generation blockchain platform with enhanced security and flexibility, was developed. As a 3^rd generation blockchain platform, it exploits BP (block producer) to increase processing speed. It has many advantages as follows. It improves both security and speed by mixing RSA (Rivest-Shamir-Adleman) and AES (advanced encryption standard). It improves flexibility by exploiting gateway to convert between apps and blockchain with different programming language. It increases processing speed by combining whole transactions into one block and distribute it when too many transactions occur. It improves search speed by inserting sequence hash into transaction data. It was implemented and applied to pet communication service and academy-instructor-student matching service, and it was verified to work correctly and effectively. Its processing speed is 3,357 transactions/second, which shows excellent performance.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1369-1373
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• 2009

Succinic acid was produced by continuous fermentation of Actinobacillus succinogenes sp. 130Z in an external membrane cell recycle reactor to improve viable cell concentration and productivity. Using this system, cell concentration increased to 16.4 g/l at the dilution rate $0.2\;h^{-1}$, up to 3 times higher than that of batch culture, and the volumetric productivity of succinic acid increased up to 6.63 g/l/h at the dilution rate $0.5\;h^{-1}$, 5 times higher than that of batch fermentation. However, in the continuous culture using a high dilution rate, operational problems including severe membrane fouling and contamination by lactic acid producer were observed. Another succinic acid producer, Mannheimia succiniciproducens MBEL55E, was also utilized in this system, and the cell concentration and productivity of succinic acid at the dilution rate of $0.3\;h^{-1}$ were found to be above 3 and 2.3 times higher, respectively, compared with those obtained at the dilution rate of $0.1\;h^{-1}$. These observations give a deep insight into the process design for a continuous succinic acid production by microorganisms.