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Optimization of Separation Process of Bioflavonoids and Dietary Fibers from Tangerine Peels using Hollow Fiber Membrane (중공사 막을 이용한 감귤 과피 bioflavonoids 분리 및 식이 섬유 회수 공정 최적화)

  • Lee, Eun-Young;Woo, Gun-Jo
    • Korean Journal of Food Science and Technology
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    • v.30 no.1
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    • pp.151-160
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    • 1998
  • Tangerine peel is mostly discarded as waste in citrus processing. However, tangerine peel contains besides dietary fibers bioflavonoids such as naringin and hesperidin which act as antimicrobials and blood pressure depressants, respectively. A continuous membrane separation process was optimized for the production of bioflavonoids relative to feed flow rate, transmembrane pressure, temperature, and pH. The tangerine peel was blended with 7.5 times water volume and the extract was prefiltered through a prefiltration system. The prefiltered extract was ultrafiltered in a hollow fiber membrane system. The flux and feed flow rate didn't show any apparent correlation, but we could observe a mass-transfer controlled region of over 8 psi. When temperature increased from $9^{\circ}C\;to\;25^{\circ}C$, the flux increased about $10\;liters/m^2/min\;(LMH)$ but between $25^{\circ}C\;and\;33^{\circ}C$, the flux increased only 2 LMH. At every transmembrane pressure, the flux of pH 4.8 was the most highest and the flux at pH 3.0 was lower than that of pH 6.0, 7.0, or 9.0. Therefore, the optimum operating conditions were 49.3 L/hr. 10 psi, $25^{\circ}C$, and pH 4.8. Under the optimum conditions, the flux gradually decreased and finally reached a steady-state after 1 hr 50 min. The amount of dietary fibers in 1.0 g retentate in each separation step was analyzed and bioflavonoids concentration in each permeate was measured. The contents of total dietary fiber in the 170 mesh retentate and soluble dietary fiber in the prefiltered retentate were the highest. Naringin and hesperidin concentration in the permeate were $0.45{\sim}0.65\;mg/g\;and\;5.15{\sim}6.86\;mg/g$ respectively, being $15{\sim}22$ times and $79{\sim}93$ times higher than those in the tangerine peel. Therefore, it can be said that PM 10 hollow fiber membrane separation system may be a very effective method for the recovery of bioflavonoids from tangerine peel.

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A Study on the Representation Characteristics of Yuanming New Garden in China by Traditional Landscape Creation Techniques (전통조경 조성 기법으로 본 중국 원명신원의 재현 특성 고찰)

  • Kim, Dong-Hyun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.1
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    • pp.103-114
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    • 2020
  • This study was conducted on Yuanming New Garden in China, which re-lighted the previously lost or damaged original space through representation from the perspective of creating traditional landscape. We looked at the composition of this place, the comparison of relevant literature and actual representation, and the characteristics of representation techniques. The results are as follows. First, TYuanming New Garden is a representation space with the motif of Yunamingyuan in Beijing. An advisory group of some 400 related experts discussed the prototype. The historicity of the real space was considered in the representation process. Second, New Yuanmingyuan garden was recreated based on the 'The Forty Scenic Views of Yuanmingyuan', and 31 of the scenic spots were created. The buildings in each precinct had a 1:1 scale response with structures that were previously constructed in Yuanmingyuan, Beijing. New Yuanmingyuan garden's way in which the main landscape is laid out around the Fuhai Lake(福海), which the landscape is drawn appeared was the same as Yuanmingyuan in Beijing. However, some of the facilities were reproduced on the basis of the 'The Forty Scenic Views of Yuanmingyuan', so they turned out to be different from what Yuanminyuan in Beijing looks like today. New Changchunyuan garden was represented around water landscape of the Western Mansins(Xiyang Lou, 西洋樓) area and the Haeakgaegum(海岳開襟), and the buildings and the facilities were reconstructed based on the 'Copper Print of Western Mansins'. Third, Yuanming New Garden made a prototype of the 'The Forty Scenic Views of Yuanmingyuan' and 'Copper Print of Western Mansins' during the process of synchronic changes in the garden. In addition, the original space and the ambiguous original space or exhibition space were clearly identified through the plant. On the other hand, due to the reenactment of buildings, the spatial composition and placeability of the original spaces of Yuanmingyuan garden and Changchunyuan garden in Beijing were not inherited, and the introduction of elements that did not match traditional landscaping spaces, and the introduction of garden elements that were not prototype and other variations for the use of tourism were found to be drawback.

The Origin of Meju Fungi - Fungal Diversity of Soybean, Rice Straw and Air for Meju Fermentation

  • Kim, Dae-Ho;Lee, Jong-kyu;Hong, Seung-Beom
    • 한국균학회소식:학술대회논문집
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    • 2014.10a
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    • pp.32-32
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    • 2014
  • Meju is a brick of dried fermented soybeans and is the core material for Jang such as Doenjang and Ganjang. Jang is produced by addition of salty water to Meju and is considered the essential sauces of authentic Korean cuisine. Meju is fermented by diverse microorganisms such as bacteria, fungi and yeasts. It is known that fungi play an important role in the Meju fermentation and they degrade macromolecules of the soybeans into small nutrient molecules. In previous study, 26 genera and 0 species were reported as Meju fungi. However, it is not comprehensively examined where the fungi present on the Meju are originated. In order to elucidate the origin of the fungi present on the Meju, the mycobiota of 500 samples soybean kernels, 296 rice straw pieces and air samples of Jang factories was determined in 0, 2 and 7 Jang factories respectively. Forty-one genera covering 86 species were isolated from the soybeans and 33 species were identical with the species from Meju. From sodium hypochlorite untreated soybeans, Eurotium herbariorum, Eurotium repens, Cladosporium tenuissimum, Fusarium fujikuroi, Aspergillus oryzae/flavus and Penicillium steckii were the predominant species. In case of sodium hypochlorite-treated soybeans, Eurotium herbariorum, E. repens and Cladosporium tenuissimum were the predominant species. Of the 4 genera and 86 species isolated from soybeans, 3 genera and 33 species were also found in Meju. Thirty-nine genera and 92 species were isolated from the rice straws and 40 species were identical with the species from Meju. Fusarium asiaticum, Cladosporium cladosporioides, Aspergillus tubingensis, A. oryzae, E. repens and Eurotium chevalieri were frequently isolated from the rice straw obtained from many factories. Twelve genera and 40 species of fungi that were isolated in the rice straw in this study, were also isolated from Meju. Especially, A. oryzae, C. cladosporioides, E. chevalieri, E. repens, F. asiaticum and Penicillium polonicum that are abundant species in Meju, were also isolated frequently from rice straw. C. cladosporioides, F. asiaticum and P. polonicum that are abundant in low temperature fermentation process of Meju fermentation, were frequently isolated from rice straw incubated at $5^{\circ}C$ and $25^{\circ}C$, while A. oryzae, E. repens and E. chevalieri that are abundant in high temperature fermentation process of Meju fermentation, were frequently isolated from rice straw incubated at $25^{\circ}C$ and $35^{\circ}C$. This suggests that the mycobiota of rice straw have a large influence in mycobiota of Meju. Thirty-nine genera and 92 species were isolated from the air of Jang factories and 34 species were identical with the species from Meju. In outside air of the fermentation room, Cladosporium sp. and Cladosporium cladosporioides were the dominant species, followed by Cladosporium tenuissimum, Eurotium sp., Phoma sp. Sistotrema brinkmannii, Alternaria sp., Aspergillus fumigatus, Schizophyllum commune, and Penicillium glabrum. In inside air of the fermentation room, Cladosporium sp., Aspergillus oryzae, Penicillium chrysogenum, A. nidulans, Aspergillus sp., C. cladosporioides, Eurotium sp., Penicillium sp., C. tenuissimum, A. niger, E. herbariorum, A. sydowii, and E. repens were collected with high frequency. The concentrations of the genus Aspergillus, Eurotium and Penicillium were significantly higher in inside air than outside air. From this results, the origin of fungi present on Meju was inferred. Of the dominant fungal species present on Meju, Lichtheimia ramosa, Mucor circinelloides, Mucor racemosus, and Scopulariopsis brevicaulis are thought to be originated from outside air, because these species are not or are rarely isolated from rice straw and soybean; however, they were detected outside air of fermentation room and are species commonly found in indoor environments. However, A. oryzae, P. polonicum, E. repens, P. solitum, and E. chevalieri, which are frequently found on Meju, are common in rice straw and could be transferred from rice straw to Meju. The fungi grow and produce abundant spores during Meju fermentation, and after the spores accumulate in the air of fermentation room, they could influence mycobiota of Meju fermentation in the following year. This could explain why concentrations of the genus Aspergillus, Eurotium, and Penicillium are much higher inside than outside of the fermentation rooms.

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Characteristics of Sand-Silt Mixtures during Freezing-Thawing by using Elastic Waves (탄성파를 이용한 모래-실트 혼합토의 동결-융해 특성)

  • Kang, Mingu;Kim, Sangyeob;Hong, Seungseo;Kim, Youngseok;Lee, Jongsub
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.5
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    • pp.47-56
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    • 2014
  • In winter season, the pore water inside the ground freezes and thaws repetitively due to the cold air temperature. When the freezing-thawing processes are repeated on the ground, the change in soil particle structure occurs and thus the damage of the infrastructure may be following. This study was performed in order to investigate the stiffness change of soils due to the freeze-thaw by using elastic waves. Sand-silt mixtures are prepared with in the silt fraction of 40 %, 60 % and 80 % in weight and in the degree of saturation of 40 %. The specimens are placed into the square freezing-thawing cell by the temping method. For the measurement of the elastic waves, a pair of the bender elements and a pair of piezo disk elements are installed on the cell, and a thermocouple is inserted into soils for the measurement of the temperature. The temperature of the mixtures is decreased from $20^{\circ}C$ to $-10^{\circ}C$ during freezing, is maintained at $-20^{\circ}C$ for 18 hours, is gradually increased up to the room temperature of $20^{\circ}C$ to thaw the specimens. The shear waves, the compressional waves and the temperature are measured during the freeze-thaw process. The experimental result indicates that the shear and the compressional wave velocities after thawing are smaller than those of before freezing. The velocity ratio of after thawing to before freezing of shear wave is smaller than that of the compressional wave. As silt fraction increases from 40 % to 80 %, the shear and compressional wave velocities are gradually increased. This study suggests that the freezing-thawing process in unsaturated soil loosens the soil particle structure, and the shear wave velocity reflects the effect of freezing-thawing more sensitively than the compressional wave velocity.

Prior Eco-preserve Zoning through Stream Ecosystem Evaluation on Dam Basin -A Case of Yongdam-dam Watershed, Jeollabukdo Province- (댐유역 하천생태계평가를 통한 생태보전우선지역설정 -용담다목적댐 유역을 사례로-)

  • Lim, Hyun-Jeong;Lee, Myung-Woo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.39 no.2
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    • pp.103-112
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    • 2011
  • The purpose of this study is to specify the prior eco-preserve zone by establishing the eco-landscape unit on the stream corridor and evaluating the stream ecosystem in the dam basin. The fundamental ecological data was surveyed and collected through "the ecosystem project on Yongdam multipurpose dam watershed" from 2008 to 2009. The Yongdam Dam Watershed has several streams, Jujacheon, Jeongjacheon and Guryangcheon, of which the area is $930km^2$, stretching to Jinangun, Jangsugun and Mujugun Jellabukdo. In spite of being used for drinking purpose, the dam water quality and ecosystem is threatened by in-watershed pollution produced by development, golf course grounds and sports complex, etc. The landscape unit of stream corridor was zoned across by 250m, 500m, and 750m from the vicinity line of stream, which was decided to the accuracy of mapping and surveying. Types of evaluation are the Stream Corridor Evaluation(SCE) and the Vegetated Area Evaluation(VAE). In the process of SCE, several indices were analysed, fish species diversity, species peculiarity, and stream naturality. Indices for VAE were forest stand map, vegetation protection grade, species diversity and peculiarity for wild bird and mammal life. The importance of the ecological items is categorized into three levels and overlapped for specifying the prior preserve zone. The area at which legally protecting species appeared is categorized as absolute preserve area. This study might be meaningful for proposing the evaluation process of a stream corridor ecosystem, which can synthesize a lot of individual ecological surveys. We hope further research will be actively performed about the ecotope mapping which is based on a individual wildlife territory and habitats and also their relationships.

Continuous Wet Oxidation of TCE over Supported Metal Oxide Catalysts (금속산화물 담지촉매상에서 연속 습식 TCE 분해반응)

  • Kim, Moon Hyeon;Choo, Kwang-Ho
    • Korean Chemical Engineering Research
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    • v.43 no.2
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    • pp.206-214
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    • 2005
  • Heterogeneously-catalyzed oxidation of aqueous phase trichloroethylene (TCE) over supported metal oxides has been conducted to establish an approach to eliminate ppm levels of organic compounds in water. A continuous flow reactor system was designed to effect predominant reaction parameters in determining catalytic activity of the catalysts for wet TCE decomposition as a model reaction. 5 wt.% $CoO_x/TiO_2$ catalyst exhibited a transient period in activity vs. on-stream time behavior, suggesting that the surface structure of the $CoO_x$ might be altered with on-stream hours; regardless, it is probable to be the most promising catalyst. Not only could the bare support be inactive for the wet decomposition reaction at $36^{\circ}C$, but no TCE removal also occurred by the process of adsorption on $TiO_2$ surface. The catalytic activity was independent of all particle sizes used, thereby representing no mass transfer limitation in intraparticle diffusion. Very low TCE conversion appeared for $TiO_2$-supported $NiO_x$ and $CrO_x$ catalysts. Wet oxidation performance of supported Cu and Fe catalysts, obtained through an incipient wetness and ion exchange technique, was dependent primarily on the kinds of the metal oxides, in addition to the acidic solid supports and the preparation routes. 5 wt.% $FeO_x/TiO_2$ catalyst gave no activity in the oxidation reaction at $36^{\circ}C$, while 1.2 wt.% Fe-MFI was active for the wet decomposition depending on time on-stream. The noticeable difference in activity of the both catalysts suggests that the Fe oxidation states involved to catalytic redox cycle during the course of reaction play a significant role in catalyzing the wet decomposition as well as in maintaining the time on-stream activity. Based on the results of different $CoO_x$ loadings and reaction temperatures for the decomposition reaction at $36^{\circ}C$ with $CoO_x/TiO_2$, the catalyst possessed an optimal $CoO_x$ amount at which higher reaction temperatures facilitated the catalytic TCE conversion. Small amounts of the active ingredient could be dissolved by acidic leaching but such a process gave no appreciable activity loss of the $CoO_x$ catalyst.

Removal Characteristics of Lead-contaminated Soil at Military Shooting Range by Using Soil Washing Process (토양세척공정을 이용한 군사격장 납 오염토양의 제거특성)

  • Ahn, Sung-Kyun;Kim, Chul;Lee, Joung-Man;Lee, Gang-Choon;Shon, Zang-Ho;Jung, Byung-Gil;Yoon, Tae-Kyung
    • Clean Technology
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    • v.18 no.4
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    • pp.390-397
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    • 2012
  • Removal characteristics of lead-contaminated soil at the military shooting range located in the Changwon city were studied experimentally using soil washing process. As a washing solution, hydrogen chloride (HCl) concentrations of 0.001, 0.01, 0.1 and 0.2 N were used, and soil : solution ratios were 1 : 2, 1 : 3, 1 : 4, and 1 : 5. Particle diameter of contaminated soil of 4-0.075 mm, and washing period of 5, 10, 15, 20, 30, 60, and 120 min were used as operating parameters. The optimum concentration of HCl solution was 0.1 N (56.3% of Pb removal efficiency) with 15 minutes operation period in views of economics, and the optimum soil : solution ratio was determined as 1 g : 3 mL for 69.7% of Pb removal efficiency with 0.1 N HCl and 15 minutes washing period. As washing period increased, removal efficiency was increased until 20 min of the removal efficiency of 75.3%, and then almost stable. Pb removal efficiency in soil particle diameters of 0.075 mm or more was ranged from 77.0% to 82.0%, but it was decreased to 52.8% in diameter of less than 0.075 mm. Therefore, the optimum cut-off size of the soil particle diameter was found less than 0.075 mm. Combined HCl solution and ultrasonic washing method showed better removal efficiency compared to only water or HCl washing method for particle sizes above 0.075 mm.

Effect of Blanching Condition, Acid and Alkali Treatments on the Qualities of Carrot Juices (Blanching 조건(條件) 및 산(酸)·알칼리 처리(處理)가 당근주우스의 품질(品質)에 미치는 영향(影響))

  • Kim, Woon Sung;Kim, Seung Yeol
    • Korean Journal of Agricultural Science
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    • v.10 no.1
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    • pp.135-145
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    • 1983
  • This study was conducted to obtain the fundamental data for the processing of carrot juices and know the effects of blanching conditions, acid and alkali treatments and sterilization on the quality factors of carrot juices. The result obtained was as follows. 1. Blanching condition, $100^{\circ}C$, 5min. was the most effective for the Brix, amino-N content, suspended solid, light transmittance and yield of carrot juices among $90^{\circ}C$, 15min., $95^{\circ}C$, 10 min., and $100^{\circ}C$, 5min. 2. 0.05N-acetic acid solution was the most effective blanching solution for the suspended solid, light transmittance, viscosity and yield of the juices compared to 0.05N-citric acid and 0.03N-hydrochloric acid solution. 3. The color changes during the processing of carrot juices caused by blanching process rather than sterilization process. 4. The ${\beta}$-carotene in carrot juices was very stable and about 80 % of it was remained in the carrot juice which had been blanched, extracted and sterilized at $115^{\circ}C$ for 30min. 5. Alkali treatment for the juice from acid - blanched carrots formed discoloration after sterilization. 6. Relative content (%) of sugars in raw carrot juice we re ribose, 8.51%; fructose. 10.15%; glucose, 12.25%; sucrose, 49.53% and oligosaccharide, 19.56%. When the carrots were blanched in boiling water, the contents of monosaccharide and disaccharide decreased slightly but that of oligosaccharide increased slightly, however, when the carrots were blanched in acid solution, and then neutralized and sterilized, relative contents of ribose and sucrose decreased remarkably but that of oligosaccharide increased considerably and those of glucose and fructose increased slightly. 7. Nineteen sorts of free amino acid were detected from the carrot juices and the mains of them were threonine+asparagine, alanine, serine+glutamine, aspartic acid, arginine, and glutamic acid.

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Feasibility Study of the Stabilization for the Arsenic Contaminated Farmland Soil by Using Amendments at Samkwang Abandoned Mine (삼광광산 주변 비소 오염 토양에 대한 안정화 공법 적용성 평가)

  • Lee, Jung-Rak;Kim, Jae-Jung;Cho, Jin-Dong;Hwang, Jin-Yeon;Lee, Min-Hee
    • Economic and Environmental Geology
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    • v.44 no.3
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    • pp.217-228
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    • 2011
  • The feasibility study for the stabilization process using 5 amendments was performed to quantify As-immobilization efficiency in farmland soils around Samkwang abandoned mine, Korea. For the batch experiments, with 2% and 3% of granular lime(2-5 mm in diameter), leaching concentration of As from the soil decreased by 86% and 95% respectively, compared to that without the amendment. When 5% and 10% of granular limestone was added in the soil, As concentration decreased by 82% and 95%, showing that lime and limestone has a great capability to immobilize As in the soil. From the results of batch experiments, continuous column(15 cm in dimeter and 100 cm in length) tests using granular lime and limestone as amendments was performed. Without the amendment, As concentration from the effluent of the column ranged from 167 ${\mu}g$/L to 845 ${\mu}g$/L, which were higher than Korea Drinking Water Limit(50 ${\mu}g$/L). However, only with 1% and 2% of lime, As concentration from the column dramatically decreased by 97% for 9 years rainfall and maintained below 50 ${\mu}g$/L. With 5% of limestone and the mixed amendment(1% of lime + 2% of limestone), more than 95% diminution of As leaching from the column occurred within I year rainfall and maintained below 20 ${\mu}g$/L, suggesting that the capability of limestone to immobilize As in the farmland soil was outstanding and similar to that of lime. Results of experiments suggested that As stabilization process using limestone could be more available to immobilize As from the soil than using lime because of low pH increase and thus less harmful side effect.

Histological and Histochemical Studies on the Cutaneous Mucous Glands According to the Development of Frog, Rana nigromaculata (개구리(Rana nigromaculata)발생에 따른 피부점액선의 조직학적 및 조직화학적 연구)

  • Kim, Han-Hwa;Noh, Yong-Tai;Chung, Young-Wha
    • The Korean Journal of Zoology
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    • v.18 no.2
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    • pp.51-60
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    • 1975
  • The present study was performed histologically and histochemically to observe the cutaneous mucous glands in the frog, Rana nigromaculata during metamorphosis. The cutaneous thssues including dermal plicae in the dorsal portions of the frog tadpoles at each metamorphosis stage were fixed in 10% buffered formalin at$4^{\circ}C$, embedded in paraffin wax, sectioned 4 $\mu$m thickness and stained with periodic acid-Schiff(PAS) and alcian blue (AB) at both pH 2.5 and pH 1.0. The results of observation were as follows: 1. The developments of cutaneous mucous glands of the frog tadpole were begun with appearance of gland cell nest in the dermis at metamorphosis XV stage and significant numerical increases could be seen at metamorphosis XX, XXIII and XXIV stages. 2. This cutaneous mucous gland of the frog tadpole could be divided into two types; A-type glands showed strong positivities to both PAS and AB at pH 2.5 in the gland body cells and to PAS in the gland neck cells, and B-type glands at AB pH 2.5 in the gland body cells. 3. In the A-type mucous glands, the reactivities of the glandular epithelial cells to both PAS and AB stain could be first seen at the metamorphosis XIX stage of frog tadpole. The reactivities of the glandular epithelial cells to both PAS and AB pH 2.5 were gradually increased according to the process of metamorphosis after XX stage of metamorphosis. 4. The B-type mucous glands were first seen at the XX stage and the reactivity of the glandular epithelial cells to AB at pH 2.5 was gradually increased according to the process of metamorphosis after XX stage. 5. The A-type and the B-type mucous glands were in the ratio of 99 : 1, 7 : 3 and 5.5 : 4.5 for each of metamorphosis XX, XXI-XXII and XXIII-XXV stages. 6. The remarkable development of the cutaneous mucous glands of the frog tadpoles might be needed to maintain water and electrolyte balances according to the change of way from aquatic life to amphibious.

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