• Genomics & Informatics
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• 제19권4호
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• pp.39.1-39.8
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• 2021

Tamoxifen (TAM) is an anticancer drug used to treat estrogen receptor (ER)-positive breast cancer. However, its ER-independent cytotoxic and antifungal activities have prompted debates on its mechanism of action. To achieve a better understanding of the ER-independent antifungal action mechanisms of TAM, we systematically identified TAM-sensitive genes through microarray screening of the heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by a spotting assay, finally yielding 13 TAM-sensitive genes under the drug-induced haploinsufficient condition. For these 13 TAM-sensitive genes, we conducted a comparative analysis of their Gene Ontology (GO) 'biological process' terms identified from other genome-wide screenings of the budding yeast deletion library and the MCF7 breast cancer cell line. Several TAM-sensitive genes overlapped between the yeast strains and MCF7 in GO terms including 'cell cycle' (cdc2, rik1, pas1, and leo1), 'signaling' (sck2, oga1, and cki3), and 'vesicle-mediated transport' (SPCC126.08c, vps54, sec72, and tvp15), suggesting their roles in the ER-independent cytotoxic effects of TAM. We recently reported that the cki3 gene with the 'signaling' GO term was related to the ER-independent antifungal action mechanisms of TAM in yeast. In this study, we report that haploinsufficiency of the essential vps54 gene, which encodes the GARP complex subunit, significantly aggravated TAM sensitivity and led to an enlarged vesicle structure in comparison with the SP286 control strain. These results strongly suggest that the vesicle-mediated transport process might be another action mechanism of the ER-independent antifungal or cytotoxic effects of TAM.

• Journal of Korean Society for Quality Management
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• 제20권1호
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• pp.136-146
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• 1992

Most of scheduling papers on FMS have been considered that the fabrication process, the machining process and the assembly process etc. are independent and the releasing, routing, dependent, batching, loading problem are treated separetely. In this paper, we discuss that the integrated scheduling problem which can be solved for efficient use. We combine two systems that each process system which in order to produce a product is treated dependently, the releasing and the routing problem are combined one system and we present the efficient flexible manufacturing system as including the alternate process.

• Journal of Korean Society of Environmental Engineers
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• 제28권7호
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• pp.752-756
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• 2006

The independent anoxic reactor was introduced in biological aerated filters as the regulation of water quality requirement, especially total nitrogen, had been strengthened. The process studied in this work was upflow $Biobead^{(R)}$ process which was used commercial invented for removal of organic materials and nitrification. For the purpose of evaluating the independent anoxic reactor, PCR-DGGE, of the molecular biological methods, was performed. Two types of nitrite reductase genes were selected. One is nirS represented cytocrome $cd_1$ nitrite reductase gene and the other is nirK represented Cu-containing nitrite reductase gene. Denitrifier community in the independent anoxic reactor was analyzed with PCR-DGGE using these two denitrifying functional genes. As the result of the PCR, only nirS gene was detected between nirS and nirK. With the result of the DGGE, specific bands became strong, as the operating days were longer, nitrate loading rate was increased. otherwise those of the initial activated sludge showed various bands. In the consequence of the sequence of DGGE bands, various denitrifiers were sequenced in the initial activated sludge, while specific denitrifiers like alcaligenes faecalis were predominant in the anoxic reactor. Consequently, introduction of the independent anoxic reactor made it possible to achieve 96% denitrification efficiency, and was proper for the modification of BAF process.

• Bulletin of the Korean Mathematical Society
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• 제40권3호
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• pp.373-383
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• 2003

We investigate a uniform local asymptotic normality for likelihood ratio processes based on an independent and identically distributed local asymptotic problem. Our tool is an empirical process theory.

• Communications for Statistical Applications and Methods
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• 제3권1호
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• pp.207-217
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• 1996

The problem of detecting change with independent data is considered. The asymptotic distribution of the sample change process with the sample Fourier coefficients is shown as a Brownian Bridge process. We suggest to use dynamic statistics such as a sample Brownian Bridge and graphs as statistical animation. Graphs including change PP plots are given by way of illustration with the simulated data.

• Korean Journal of Optics and Photonics
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• 제14권4호
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• pp.359-364
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• 2003

We propose a novel vertical directional coupler with polarization independent very short coupling lengths using the double-sided deep-ridge waveguide structure which could be implemented using double-sided process to polarization insensitive deep-ridge waveguide structures and investigate the effect of various structure parameters on the coupling length. Variation of coupling length for the variation of the waveguide width is smaller than that for the variation of the core thickness. Coupling length decreases as the inner cladding layer thickness and the core thickness decrease. The waveguide width with the polarization independent coupling length decreases as the inner cladding layer thickness decreases for the same core thickness and the core thickness decreases for the same inner cladding layer thickness.

• Korean Journal of Social Welfare
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• 제62권2호
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• pp.375-397
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• 2010

Previous studies on empowerment have commonly noted that current practice of empowerment understands empowerment only macroscopically and limitedly without reflecting the true idea of empowerment. In addition, they are emphasizing lack of information on specific techniques for the practice. Thus, the present study purposed to examine independent living movement for the disabled, which have experienced structural and institutional changes and produced actual improvements in policies as their results, and the characteristics of empowerment observed in the development process. According to the results of this study, empowerment consists of a total of five stages, and among them, the stage of conscientization is very important for individual empowerment and the stage of organization for macroscopic empowerment.

• Transactions of the Korean Society of Mechanical Engineers A
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• 제39권3호
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• pp.327-335
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• 2015

In this paper, we propose an independent cooling control (ICC) scheme for high strength and atmosphere corrosion resisting steel to obtain the desired temperature and properties along the longitudinal direction of the steel in the run-out table (ROT) process. A temperature model of the independent process is developed to divide the ROT into front and back sections. The control concept uses field data, problem analysis, and a time-temperature transformation diagram. The effectiveness of the proposed control is verified using simulation results under a temperature disturbance by the transformation in the middle of the ROT. The results of a hot strip mill field test show that the temperature control performance is significantly improved by the proposed control scheme.

• Korean Institute of Interior Design Journal
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• 제18권2호
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• pp.93-101
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• 2009

This study is including the independent project, "building children's library of the town" that is conducted by a local resident. The study object is the contents of the "design workshop for library planning" and the general process during one year since the opening. This study is delivering "information of children's library" through the work-shop and is conducting the role of a professional space planner by measuring the blueprint. Thus, the study could be conducted with synthetic viewpoint on each library. The followings are the results from the study. First, there were various space operations and a variety of programs in the case library that is independently managed. This is directing to "the constructive and active space usage" by the users and finally results in positive circulation. Secondly, there is a change through the process of execution and operation in the library construction plan. There are needs for the flexible space expansion and adjustment, modifiable furniture layout and design and various keeping areas because there is on-going tendency of corresponding space change. The third, the author could draw more practicable consideration points for space planning by looking at the trend of space change in Transactionist viewpoint. Thus, this study is very meaningful in terms of planning of sustainable public areas.

• Environmental Engineering Research
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• 제11권2호
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• pp.63-76
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• 2006

Multivariate analysis and batch monitoring on a pilot-scale sequencing batch reactor (SBR) are described for integrated wastewater treatment management system, where a batchwise multiway independent component analysis method (MICA) are used to extract meaningful hidden information from non-Gaussian wastewater treatment data. Three-way batch data of SBR are unfolded batch-wisely, and then a non-Gaussian multivariate monitoring method is used to capture the non-Gaussian characteristics of normal batches in biological wastewater treatment plant. It is successfully applied to an 80L SBR for biological wastewater treatment, which is characterized by a variety of error sources with non-Gaussian characteristics. The batchwise multivariate monitoring results of a pilot-scale SBR for integrated wastewater treatment management system showed more powerful monitoring performance on a WWTP application than the conventional method since it can extract non-Gaussian source signals which are independent and cross-correlation of variables.