• Journal of Microbiology and Biotechnology
• /
• 제28권4호
• /
• pp.551-560
• /
• 2018

Bellflower root (Platycodon grandiflorum), which belongs to the Campanulaceae family, is a perennial grass that grows naturally in Korea, northeastern China, and Japan. Bellflower is widely consumed as both food and medicine owing to its high nutritional value and potential therapeutic effects. Since foodborne disease outbreaks often come from vegetables, understanding the public health risk of microorganisms on fresh vegetables is pivotal to predict and prevent foodborne disease outbreaks. We investigated the microbial communities on the bellflower root (n = 10). 16S rRNA gene amplicon sequencing targeting the V6-V9 regions of 16S rRNA genes was conducted via the 454-Titanium platform. The sequence quality was checked and phylogenetic assessments were performed using the RDP classifier implemented in QIIME with a bootstrap cutoff of 80%. Principal coordinate analysis was performed using the weighted Fast UniFrac distance. The average number of sequence reads generated per sample was 67,192 sequences. At the phylum level, bacterial communities from the bellflower root were composed primarily of Proteobacteria, Firmicutes, and Actinobacteria in March and September samples. Genera Serratia, Pseudomonas, and Pantoea comprised more than 54% of the total bellflower root bacteria. Principal coordinate analysis plots demonstrated that the microbial community of bellflower root in March samples was different from those in September samples. Potential pathogenic genera, such as Pantoea, were detected in bellflower root samples. Even though further studies will be required to determine if these species are associated with foodborne illness, our results indicate that the 16S rRNA gene-based sequencing approach can be used to detect pathogenic bacteria on fresh vegetables.

• 정보과학회 논문지
• /
• 제44권4호
• /
• pp.392-398
• /
• 2017

포인트 클라우드를 이용한 물체의 표현은 레이저 스캐너를 통해 공간을 스캔하여 점의 집합을 추출하고, 정합(Registration)을 통해 하나의 좌표계로 통합하는 과정을 거쳐 이루어진다. 정합이 완료된 포인트 클라우드 집합은 수학적 해석을 통해 의미 있는 영역, 형태, 잡음 등으로 분류되어 쓰이게 된다. 본 논문은 3차원 포인트 클라우드 데이터에서 실린더 형태의 굽은 영역 매칭을 목표로 한다. 매칭 절차는 포인트 클라우드에서 RANdom SAmple Consensus(RANSAC)을 통한 구(sphere) 적합(fitting)으로 실린더 형태의 점 후보군을 추출하여 중심과 반지름 데이터를 얻고, 추출된 중심점 데이터에서 주성분 분석(Principal Component Analysis)을 통해 굽은 영역인지 판별한 후 캣멀롬 스플라인(Catmull-Rom spline)으로 굽은 영역 매칭을 완료한다. 제안된 방법은 제약조건 및 분할 없이 중심축 추정에 이은 직선 및 굽은 형태의 실린더 추정으로 비교적 빠른 추정결과를 도출하고, 역설계의 작업효율을 높일 수 있을 것으로 기대된다.

• 한국약용작물학회지
• /
• 제21권4호
• /
• pp.247-254
• /
• 2013

Collected germplasms of five representative dandelion species (Taraxacum ohwianum, T. platycarpum, T. platypecidum, T. officinale, and T. coreanum) were 104 lines from different habitates in Korea and China. Their genetic diversity was analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 1,176 total DNA fragments and 523 polymorphic bands with a 44.4% ratio of polymorphism. On the basis of similarity coefficient analysis by unweight pair group method with arithmetic averages (UPGMA), 104 dandelion germplasm lines were ranged from 0.64 to 0.99 and clustered distinct five group depending on the species. Furthermore, a principal coordinate analysis (PCA) by the application of multi-variate analysis indicated significantly greater differences among species than geographical origins.

• 한국약용작물학회지
• /
• 제21권6호
• /
• pp.455-460
• /
• 2013

Collected germplasms of five representative species belonging to Curcuma genus (C. longa, C. aromatica, C. zedoaria, C. phaeocaulis and C. kwangsiensis) were 52 samples from different farmhouse in Korea and China. To elucidate the genetic diversity among the species, 52 samples were analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 643 total DNA fragments and 349 polymorphic bands with the 54.3% ratio of polymorphism. In the analysis of coefficient similarity using unweight pair group method with arithmetic averages (UPGMA), 52 Curcuma germplasm lines were ranged from 0.60 to 0.99 and clustered distinct five groups according to the species and collected geographical levels. However, the result of principal coordinate analysis (PCA) by multi-variate analysis was shown significantly greater differences among species than geographical origins based on AFLP profiling data of these samples.

• Structural Engineering and Mechanics
• /
• 제31권6호
• /
• pp.625-638
• /
• 2009

Applications of membrane mechanisms are widely found in nano-devices and nano-sensor technologies nowadays. An alternative approach for large deflection analysis of the orthotropic, elliptic membranes - subject to gravitational, uniform pressures often found in nano-sensors - is described in this paper. The material properties of membranes are assumed to be orthogonally isotropic and linearly elastic, while the principal directions of elasticity are parallel to the coordinate axes. Formulating the potential energy functional of the orthotropic, elliptic membranes involves the strain energy that is attributed to inplane stress resultant and the potential energy due to applied pressures. In the solution method, Rayleigh-Ritz method can be used successfully to minimize the resulting total potential energy generated. The set of equilibrium equations was solved subsequently by Newton-Raphson. The unparalleled model formulation capable of analyzing the large deflections of both circular and elliptic membranes is verified by making numerical comparisons with existing results of circular membranes as well as finite element solutions. The results are found in excellent agreements at all cases. Then, the parametric investigations are given to delineate the impacts of the aspect ratios and orthotropic elasticity on large static tensions and deformations of the orthotropic, elliptic membranes.

• Advances in Computational Design
• /
• 제9권1호
• /
• pp.39-52
• /
• 2024

If the governing differential equation arising from engineering problems is treated as an analytic, continuous and derivable function, it can be expanded by one point as a series of finite numbers. For the function to be zero for each value of its domain, the coefficients of each term of the same power must be zero. This results in a recursive relationship which, after applying the natural conditions or the boundary conditions, makes it possible to obtain the values of the derivatives of the function with acceptable accuracy. The elastoplastic analysis of an inhomogeneous thick sphere of metallic materials with linear variation of the modulus of elasticity, yield stress and Poisson's ratio as a function of radius subjected to internal pressure is presented. The Beltrami-Michell equation is established by combining equilibrium, compatibility and constitutive equations. Assuming axisymmetric conditions, the spherical coordinate parameters can be used as principal stress axes. Since there is no analytical solution, the natural boundary conditions are applied and the governing equations are solved using a proposed new method. The maximum effective stress of the von Mises yield criterion occurs at the inner surface; therefore, the negative sign of the linear yield stress gradation parameter should be considered to calculate the optimal yield pressure. The numerical examples are performed and the plots of the numerical results are presented. The validation of the numerical results is observed by modeling the elastoplastic heterogeneous thick sphere as a pressurized multilayer composite reservoir in Abaqus software. The subroutine USDFLD was additionally written to model the continuous gradation of the material.

• Animal Bioscience
• /
• 제35권9호
• /
• pp.1314-1326
• /
• 2022

Objective: Alongside the rise of animal-protection awareness in Taiwan, the public has been paying more attention to dog genetic deficiencies due to inbreeding in the pet market. The goal of this study was to isolate novel microsatellite markers for monitoring the genetic structure of domestic dog populations in Taiwan. Methods: A total of 113 DNA samples from three dog breeds-beagles (BEs), bichons (BIs), and schnauzers (SCs)-were used in subsequent polymorphic tests applying the 14 novel microsatellite markers that were isolated in this study. Results: The results showed that the high level of genetic diversity observed in these novel microsatellite markers provided strong discriminatory power. The estimated probability of identity (P_(ID)) and the probability of identity among sibs (P_(ID)sib) for the 14 novel microsatellite markers were 1.7×10^-12 and 1.6×10^-5, respectively. Furthermore, the power of exclusion for the 14 novel microsatellite markers was 99.98%. The neighbor-joining trees constructed among the three breeds indicated that the 14 sets of novel microsatellite markers were sufficient to correctly cluster the BEs, BIs, and SCs. The principal coordinate analysis plot showed that the dogs could be accurately separated by these 14 loci based on different breeds; moreover, the Beagles from different sources were also distinguished. The first, the second, and the third principal coordinates could be used to explain 44.15%, 26.35%, and 19.97% of the genetic variation. Conclusion: The results of this study could enable powerful monitoring of the genetic structure of domestic dog populations in Taiwan.

• The Plant Pathology Journal
• /
• 제40권2호
• /
• pp.205-217
• /
• 2024

Brown rot disease, caused by Monilinia spp., poses a significant threat to pome and stone fruit crops globally, resulting in substantial economic losses during pre- and post-harvest stages. Monilinia fructigena, M. laxa, and M. fructicola are identified as the key agents responsible for brown rot disease. In this study, we employed the amplified fragment length polymorphism (AFLP) method to assess the genetic diversity of 86 strains of Monilinia spp. isolated from major stone fruit cultivation regions in South Korea. Specifically, strains were collected from Chungcheong, Gangwon, Gyeonggi, Gyeongsang, and Jeolla provinces (-do). A comparative analysis of strain characteristics, such as isolation locations, host plants, and responses to chemical fungicides, was conducted. AFLP phylogenetic classification using 20 primer pairs revealed the presence of three distinct groups, with strains from Jeolla province consistently forming a separate group at a high frequency. Furthermore, M. fructicola was divided into three groups by the AFLP pattern. Principal coordinate analysis and PERMANOVA were applied to compare strain information, such as origin, host, and fungicide sensitivity, revealing significant partition patterns for AFLP according to geographic origin and host plants. This study represents the utilization of AFLP methodology to investigate the genetic variability among M. fructicola isolates, highlighting the importance of continuous monitoring and management of variations in the brown rot pathogen.

• Journal of Ginseng Research
• /
• 제38권2호
• /
• pp.106-115
• /
• 2014

Background: Gut microbiota is regarded as one of the major factors involved in the control of body weight. The antiobesity effects of ginseng and its main constituents have been demonstrated, but the effects on gut microbiota are still unknown. Methods: To investigate the effect of ginseng on gut microbiota, 10 obese middle-aged Korean women took Panax ginseng extracts for 8 wk and assessment of body composition parameters, metabolic biomarkers, and gut microbiota composition was performed using 16S rRNA gene-based pyrosequencing at baseline and at 8 wk. Significant changes were observed in body weight and body mass index; however, slight changes were observed in gut microbiota. We divided the participants into two groups, the effective and the ineffective weight loss groups, depending on weight loss effect, in order to determine whether the antiobesity effect was influenced by the composition of gut microbiota, and the composition of gut microbiota was compared between the two groups. Results: Prior to ginseng intake, significant differences of gut microbiota were observed between both at phyla and genera and the gut microbiota of the effective and ineffective weight loss groups was segregated on a principal coordinate analysis plot. Conclusion: Results of this study indicate that ginseng exerted a weight loss effect and slight effects on gut microbiota in all participants. In addition, its antiobesity effects differed depending on the composition of gut microbiota prior to ginseng intake.

• 대한의용생체공학회:의공학회지
• /
• 제15권2호
• /
• pp.217-224
• /
• 1994

In order to study the shape and dimensions of heart, a procedure to reconstruct a three dimensional left ventricular geometry from two dimensional echocardiographic images was studied including the coordinate transformation, curve fitting and interpolation utilizing three dimensional position registration arm. Nonlinear material property of the left ventricular myocardium was obtained by finite element method performed on the reconstructed geometry and by optimization techniques which compared the computer predicted 3D deformation with the experimentally determined deformation. Elastic modulus ranged from 3.5g/$cm^2$ at early diastole to l53g/$cm^2$ at around end diastole showing slightly nonlinear relationship between the modulus and the pressure. Afterwards using the obtained nonlinear material propertry the stress distribution related with oxyzen consumption rate was analyzed. The maximum and minimum of ${\sigma}_1$ (max. principal stress) occurred at nodes on the second level intersection points of x-axis with endocardium and with epicardium, respectively. And the tendency of the interventricular septum to be flattened was observed from the compressive ${\sigma}_1$ on the anterior, posterior nodes of left ventricle and from the most significant change of dimension in $D_{RL}$ (septal-lateral dimension of right ventricle).