• Title/Summary/Keyword: priming solution

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The Effect of Deep Sea Water on Seed Priming of Sweet Pepper (Capsicum annum L.), Rice (Oryza sativa L.) and Ginseng (Panax ginseng C.A.Meyer)

  • Yoon Byeong-Sung;Shrestha Surendra Lal;Kang Won-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.3
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    • pp.411-417
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    • 2006
  • This experiment was conducted to study whether priming with deep sea water results in enhancement of seed germination and to identify the optimum concentration of the priming solution, and duration of priming using sweet pepper (Cv. California wonder), rice (Cv. Ilpum) and ginseng seed. Sweet pepper and rice seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) for deep sea water for 48 hours, 24 hours and 12 hours at $25^{\circ}C$ and ginseng seeds in 5%, 10%, 15%, 20%, 25% and 30%, and 2,4,6, and 8 electrical conductivity (EC) which were made by desalinating deep sea water. Priming in deep sea water (DSW) improved the early and final germination percentage, mean germinal on rate, emergence percentage and root and shoot length, compared with plain water, $KNO_3$ and without priming treatments. In sweet pepper, 24 hours priming with 5 percentage DSW significantly improved the early germination percentage and radical length. It has also improved the mean germination and emergence days and early emergence percentage, compared with $KNO_3$ and control. Whereas, in rice, 48 hours priming with 10 percent DSW significantly improved the early germination percentage, plumule emergence percentage, root length and shoot height. Hence the best seed priming treatment on sweet pepper and Rice are 24 hours with 5 percentage DSW and 48 hours with 10 percentage DSW, respectively, whereas in ginseng, priming with EC4, EC8 and 25% DSW had shown better germination.

Studies on Practical Methods to Control Seed Vigour in Several Food Crops (주요식량작물 종자세 개선에 관한 연구)

  • 김진기
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.2
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    • pp.128-138
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    • 1994
  • To improve seed vigour in rice, barley and soybean, several methods of presowing treatment, using chemicals and priming in polyethylene glycol solution, were investigated. Gibberellic acid($GA_3$) slightly improved germination of rice, but other chemical treatments showed no beneficial effect on seed vigour. Aged seeds were primed in polyethylene glycol solution then rinsed and germinated with drying back. In general mean germination time increased and percentage germination decreased with in- creasing water potential of the priming solution, but there were no significant effects on spread of germination times. Priming also showed no marked improvement in germination under cold, wet, or osmotic conditions. None of the treatments used was successful in practically improving the seed germination and vigour of the tested crops. However, seed treated with GA$_3$ gave the best overall germination response.

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Changes in Metabolites and Embryo Growth during Seeds Priming in Tobacco

  • Min, Tai-Gi;Seou, Byung-Moon;Lee, Suk-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.3
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    • pp.273-276
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    • 1999
  • Some metabolites and embryo growth of primed tobacco (Nicotiana tabacum L. cv. ‘KFI09’) seeds were observed during priming. The seeds were primed at 15 and $25^{\circ}C$ for 1, 2, 3, 5, 10 and 15 days in a -0.8 MPa polyethylene glycol 6000 (PEG) solution. The time to 50% seed germination (T$_{50}$) was greatly reduced when the seeds were primed at $25^{\circ}C$ when compared with 15$^{\circ}C$. The $\alpha$-amylase activity and sugars and amino acid contents in the seeds primed at $25^{\circ}C$ greatly increased, while $\alpha$-amylase activity was similar, and sugar and amino acid contents increased slightly in the seeds primed at 15$^{\circ}C$. When the seeds were primed at $25^{\circ}C$, growth of the embryo which was enclosed by endosperm was detected, while the endosperm became thinner as the priming duration was extended.d.

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Priming Effects on Germination of Aged Tobacco Seeds

  • Min, Tai-Gi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.4
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    • pp.325-327
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    • 2001
  • Tobacco (Nicotiana tabacum L. cv KF109) seeds were artificially aged in a controlled environment of 45$^{\circ}C$ and 80% relative humidity condition for different duration up to 14 days before priming in polyethylene glycol 6000 solution of equivalent osmotic potential of -0.8 MPa for 8 days at $25^{\circ}C$. The seeds aged only and primed after aging were germinated at 15$^{\circ}C$ and $25^{\circ}C$ to observe the priming effects on the germination of aged seeds at different temperature. The germination percentage of the aged seeds was rapidly dropped starting from 8 days of aging and mean germination time (T$_{50}$) was greatly increased, particularly in germination at 15$^{\circ}C$. The germination capacity was greatly restored in the primed seeds after aging, particularly in the seeds of longer aging and germinating at 15$^{\circ}C$.>.

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Effect of Priming Treatment Using PEG on Seed Germination in Acer mono Max. (PEG를 이용한 priming 처리가 고로쇠나무 종자 발아에 미치는 영향)

  • Tak, Woo-Sik;Cho, Kyeong-Jin;Choi, Chung-Ho
    • Korean Journal of Plant Resources
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    • v.19 no.4
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    • pp.480-487
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    • 2006
  • This study was conducted to improve the germination percent and germination speed by seed priming using polyethylene glycol (PEG) 8,000 solution on Acer mono seed because it has low and irregular germination characteristics. Seed priming was carried out under 0, -0.25, -0.5, -1.0 and -2.0 MPa of water potential at $20^{\circ}C$ for 3 days. Primed seeds showed higher percent germination, germination speed, mean germination time (MGT) and germination performance index (GPI) than unprimed seeds. Especially, primed seed with -2.0 MPa of water potential had the highest germination properties. And primed seeds had a lower dormancy than the unprimed seeds. Relative growth rate and T/R ratio of seedling from primed seeds were measured to survey the effect of seed priming on the seedling vigor as well as seed germination. The seedlings from primed seeds at -2.0 MPa of water potential showed the highest relative growth rates of height and root collar diameter, however, the lowest value in T/R ratio. This study suggested that seeds priming at -2.0 MPa of water potential under $20^{\circ}C$ for 3 days enhanced not only the percent germination, germination speed, MGT and GPI but also the vigor and growth of seedlings in Acer mono.

Effect of Preservation Period, Light, Temperature, and Priming on the Seed Germination of Lysimachia mauritiana (자생 갯까치 수영(Lysimachia mauritiana)의 종자발아에 미치는 저장기간, 광, 온도 및 Priming 처리의 영향)

  • 안영희;설종호;조근호
    • Korean Journal of Environment and Ecology
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    • v.12 no.1
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    • pp.9-13
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    • 1998
  • Because Lysimachia mauritiana is not able to maintain the seed viablity for a long time, it has difficulties in using for the ornamental plants or landscape gardening. This study was conducted to elucidate the optimal germination conditions of Lysimachia mauritiana seeds. The germination rate of the seeds was higer in light condition than in dark one. In experment determining the proper germination temperature, the germination rates were 2.2%, 96.9% and 91.2% under 35$\circ $C, 25$\circ $C, and 20$\circ $C, respectively in light condition. The approprate temperature for the germination was 25$\circ $C in light. The decreases of germination rate with the increase of the preservation periods was able to recover by priming pretreated with NaCl solution and KNO$_{3}$ + K$_{3}$H$_{2}$PO mixed solutions. When the storage period was 300 days, the germination rate was 75% without priming treatments. But the seeds primed with NaCl 0.1M and KNO$_{3}$ + K$_{3}$H$_{2}$PO mixed solutions 0.1M, showed germination rate above 90%. T$_{50}$ also was shorten from 5.25 without priming to 3.78 with NaCl 0.1M. Seed priming may induce earlier sprouts and increase germination rate of long-preserved seeds of native wild plants.

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Effect of GA3, Moist Chilling Storage and Priming Treatment on Seed Germination of Caltha palustris var. membranacea Turcz (GA3, 냉습저장, 무기염 처리가 동의나물 종자의 발아에 미치는 영향)

  • La, Young Jin;Jeong, Jeong Hag
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.174-178
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    • 2008
  • This study was carried out to investigate the effect $GA_3$, moist chilling storage and priming treatments on seed germination of Caltha palustris var. membranacea. Percent germination was greatly improved by $GA_3$ treatment. However, no significant differences were observed among concentrations and soaking periods of $GA_3$. Optimum $GA_3$ treatment was one hour presoaking in a 100mg${\cdot}L^{-1}$ solution. Germination enhancing effect of moist chilling stroage was significantly greater than that of $GA_3$. Chilling treatment for five weeks resulted in the greatest percent germination of 60.3%. Unlike the role of $GA_3$ and moist chilling storage, no appreciable effect on seed germination was observed by the priming the seeds with either a $Ca(NO_3)_2 $ or $KNO_3$ solution.

Study of using Waste Industrial Catalyst for the Removal of Harmful Organic Compounds (유해 유기화합물의 제거를 위한 폐 산업용 촉매의 이용에 관한 연구)

  • Seo, Seong-Gyu;Kim, Sang-Chai
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.5
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    • pp.663-670
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    • 2004
  • The catalytic oxidation of benzene, toluene and xylene over a spent industrial catalyst (Pd-based) was investigated in a fixed bed flow reactor system. According to the priming condition, the properties of a spent Pd-based catalyst were characterized by XRD(X-ray diffraction). BET(Brunauer-Emmett-Teller) and ICP(Inductively coupled plasma). When air was used as a primer, optimum priming temperature was found to be 200$^{\circ}C$, and the catalytic activity decreased as the priming temperature increased. When a spent Pd-based catalyst primed with air at 200$^{\circ}C$ was re-treated with hydrogen at 200$^{\circ}C$, 300$^{\circ}C$ or 400$^{\circ}C$, respectively, the catalytic activity increased and thermal effect were negligible. $HNO_3$ aqueous solution priming resulted in slight decrease of the catalytic activity, with little effects on $HNO_3$ concentrations. The activity of a spent Pd-based catalyst with respect to VOC molecule was observed to follow sequence: xylene> toluene> benzene. Benzene. toluene and xylene could be removed to almost 100% by a spent Pd-based catalyst primed with hydrogen.

Morphological Change, Sugar Content, and $\alpha$-amylase Activity of Rice Seeds under Various Priming Conditions

  • Lee, Suk-Soon;Kim, Jae-Hyeun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.138-142
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    • 1999
  • An experiment was carried out to find out the changes in morphology, sugars, and $\alpha$-amylase activity during the priming of rice seeds (Oryza sativa L. cv. 'Ilpumbyeo'). For priming, seeds were soaked in -0.6 MPa PEG solution at 15$^{\circ}C$ for 4 days (properly primed) and at $25^{\circ}C$ for 4 and 10 days (over-primed) and dried at room temperature. The size of coleoptile and differentiated leaves of properly primed seeds were bigger and coleoptile was separated from the other part of embryo compared with non-primed and over-primed seeds. As priming of seeds advanced, compound starch grains in the endosperm disintegrated into tiny starch granules, and small holes were found in the tiny starch granules and a cavities developed between embryo and endosperm. The radicle and plumule of properly primed germinating seeds developed faster than non-primed and overprimed germinating seeds. Sucrose, maltose, and raffinose contents of properly primed seeds decreased, while content of glucose and fructose and $\alpha$-amylase activity increased. However, sugar content and $\alpha$-amylase activity of over-primed seeds were lower compared with non-primed seeds or properly primed seeds.

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Clinical Effect of Albumin Addition to the Prime solution in Cardiopulmonary Bypass (체외순환시 산화기 충전액에 첨가된 알부민의 임상적 효과)

  • Lee, Hyeong-Gyo;Yu, Se-Yeong
    • Journal of Chest Surgery
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    • v.23 no.6
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    • pp.1152-1157
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    • 1990
  • Colloid solution is commonly used to increase the oncotic pressures of priming solutions used in the cardiopulmonary bypass circuit. To study the effectiveness of this practice, we retrospectively evaluated 24 patients undergoing cardiac operations to receive isoelectric solution plus 50 gm of albumin[group A], isoelectric solution plus 25 gm of albumin[group B] and isoelectric solution without albumin[group C] as the prime solution for the bypass circuit. Various clinical parameters related to the perioperative fluid balance, cardiopulmonary function, and renal function were studied. There were no differences between the three groups with regard to postoperative clinical parameter of cardiopulmonary, liver function and renal function. But at the previous report by Cho et al. [38], there was a significant difference between groups with regard to hematologic property, especially in crenated red blood cells. These hematologic events did not affect the clinical outcome but thorough evaluations are needed in order to prove the clinical effect of crenated red blood cell. We conclude that there is no clinically detectable advantage for the routinely supplementing of albumin to the priming solution of bypass circuit in cardiac operations. Whether this practice can be of value in selected cases needs to be further studied.

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