• Asian-Australasian Journal of Animal Sciences
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• 제25권3호
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• pp.375-381
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• 2012

Two experiments were conducted to establish a digestible energy (DE) content prediction model of rapeseed meal for growing-finishing pig based on rapeseed meal's chemical composition. In experiment 1, observed linear relationships between the determined DE content of 22 rapeseed meal calibration samples and proximate nutrients, gross energy (GE) and neutral detergent fiber (NDF) were used to develop the DE prediction model. In experiment 2, 4 samples of rapeseed meal selected at random from the primary rapeseed growing regions of China were used for testing the accuracy of DE prediction models. The results indicated that the DE was negatively correlated with NDF (r = -0.86) and acid detergent fiber (ADF) (r = -0.73) contents, and moderately correlated with gross energy (GE; r = 0.56) content in rapeseed meal calibration samples. In contrast, no significant correlations were found for crude protein, ether extract, crude fiber and ash contents. According to the regression analysis, NDF or both NDF and GE were found to be useful for the DE prediction models. Two prediction models: DE = 16.775-0.147${\times}$NDF ($R^2$ = 0.73) and DE = 11.848-0.131${\times}$NDF+0.231${\times}$GE ($R^2$ = 0.76) were obtained. The maximum absolute difference between the in vivo DE determinations and the predicted DE values was 0.62 MJ/kg and the relative difference was 5.21%. Therefore, it was concluded that, for growing-finishing pigs, these two prediction models could be used to predict the DE content of rapeseed meal with acceptable accuracy.

• Korean Journal of Geomatics
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• 제5권1호
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• pp.7-19
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• 2005

Photogrammetric mapping procedures have gone through major developments due to significant improvements in its underlying technologies. The availability of GPS/INS systems greatly assist in direct geo-referencing of the acquired imagery. Still, photogrammetric datasets taken without the aid of positioning and navigation systems need control information for the purpose of surface reconstruction. Point features were, and still are, the primary source of control for the photogrammetric triangulation although other higher-order features are available and can be used. LIDAR systems supply dense geometric surface information in the form of three dimensional coordinates with respect to certain reference system. Considering the accuracy improvement of LIDAR systems in the recent years, LIDAR data is considered a viable supply of photogrammetric control. To exploit LIDAR data, new challenges are poised concerning the representation and reference system by which both the photogrammetric and LIDAR datasets are described. In this paper, registration methodologies will be devised for the purpose of integrating the LIDAR data into the photogrammetric triangulation. Such registration methodologies have to deal with three issues: registration primitives, transformation parameters, and similarity measures. Two methodologies will be introduced that utilize straight-line and areal features derived from both datasets as the registration primitives. The first methodology directly incorporates the LIDAR lines as control information in the photogrammetric triangulation, while in the second methodology, LIDAR patches are used to produce and align the photogrammetric model. Also, camera self-calibration experiments were conducted on simulated and real data to test the feasibility of using LIDAR patches for this purpose.

• 분석과학
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• 제18권3호
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• pp.216-223
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• 2005

연구에서는 일본의 공정시험법에 따라 7종의 아민 표준물질로부터 검량선을 작성하고 실제 공기 중의 아민시료를 채취하여 농도를 산정하였다. 검량선은 좋은 직선상관을 보였으며, 50 L의 시료채취시 트리메틸아민의 경우 검량한계가 0.033 ppb(0.040 ng)로 나타나 부지경계선에서의 규제농도(공업지역 20 ppb, 기타지역 5 ppb)까지 검출 가능함을 확인할 수 있었다. 실제 측정사례로서 하수처리장과 랜더링사업장에서 채취된 시료를 분석한 결과 methyl amine, dimethyl amine, trimethyl amine, i-propyl amine의 4종류의 아민류가 검출되었다. 하수처리장 A의 최초 침전지와 탈수실에서는 trimethyl amine이 각각 9.07 ppb, 7.79 ppb로 악취세기 2.5를 넘는 농도로 검출되었고 하수처리장 B의 폭기조와 분뇨투입동, 분뇨처리장의 기계실에서는 모두 dimethyl amine과 trimethyl amine이 평균 70.0 ppb, 2.44 ppb로 검출되어 악취에 대한 기여가 높다는 것을 알 수 있었다.

• Journal of Preventive Medicine and Public Health
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• 제39권2호
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• pp.165-170
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• 2006

Objectives : The determination of oxalate in urine is required for the diagnosis and treatment of primary hyperoxaluria, idiopathic stone disease and various intestinal diseases. We examined the possibility of using Fourier transform near-infrared (FT-NIR) spectroscopy analysis to quantitate urinary oxalate. The practical advantages of this method include ease of the sample preparation and operation technique, the absence of sample pre-treatments, rapid determination and noninvasiveness. Methods : The range of oxalate concentration in standard urine solutions was $0-221mg/{\ell}$. These 80 different samples were scanned in the region of 780-1,300 nm with a 0.5 nm data interval by a Spectrum One NTS FT-NIR spectrometer. PCR, PLSR and MLR regression models were used to calculate and evaluate the calibration equation. Results : The PCR and PLSR calibration models were obtained from the spectral data and they are exactly same. The standard error of estimation (SEE) and the % variance were $10.34mg/{\ell}$ and 97.86%, respectively. After full cross validation of this model, the standard error of estimation was $5,287mg/{\ell}$, which was much smaller than that of the pre-validation. Furthermore, the MCC (multiple correlation coefficient) was 0.998, which was compatible with the 0.923 or 0.999 obtained from the previous enzymatic methods. Conclusions : These results showed that FT-NIR spectroscopy can be used for rapid determination of the concentration of oxalate in human urine samples.

• 동의생리병리학회지
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• 제29권2호
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• pp.168-173
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• 2015

Bangpungtongseong-san(BPTSS, Fangfengtongsheng-san in Chineses) is a traditional herbal formula comprising 18 medicinal herbs. In the present study, we performed the simultaneous analysis for four compounds of BPTSS and examined anti-allergic effects in human keratinocytes and mouse splenocytes. The column for separation of four compounds was used Luna C18 column and maintained at 40℃. The mobile phase for gradient elution consisted of two solvent systems. The analysis was carried out at a flow rate of 1.0 mL/min with PDA detection at 254 and 280 nm. To evaluate production and expression of Th2 chemokines, ELISA and RT-PCR were conducted in tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated HaCaT cells with or without BPTSS or silymarin, a positive control for skin inflammation. To measure Th2 cytokines, primary mouse splenocytes were treated with BPTSS and performed ELISA for interleukin (IL)-4, 5, 13. Calibration curves were acquired with r2>0.9999. The contents of geniposide, liquiritin, baicalin, and glycyrrhizin in BPTSS were 5.06 ㎎/g, 7.33 ㎎/g, 27.56 ㎎/g, and 7.81 ㎎/g, respectively. BPTSS reduced TARC and RANTES production and mRNA expression in TNF-α and IFN-γ-treated HaCaT cells. BPTSS inhibited IL-4, 5, and 13 production in mouse splenocytes. Our data will be a helpful information to upgrade quality control and anti-allergic effects of BPTSS.

• 생약학회지
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• 제46권2호
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• pp.160-166
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• 2015

Yijung-tang (YJT) is a traditional herbal formula comprising 4 medicinal herbs. In the present study, we performed the simultaneous analysis for three compounds of YJT and examined anti-allergic effects in vitro. The column for separation of three compounds was used Gemini C18 column and maintained at 40$^{\circ}C$. The mobile phase for gradient elution consisted of two solvent systems. To evaluate Th2 chemokines, YJT was treated into tumor necrosis factor (TNF)-${\alpha}$ and interferon (IFN)-${\gamma}$-stimulated HaCaT cells, and performed ELISA for thymus and activation regulated chemokine (TARC) and regulated on activation, normal T-cell expressed and secreted (RANTES). To measure Th2 cytokines, YJT was added into primary mouse splenocytes, and performed ELISA for interleukin (IL)-4, 5, 13. Calibration curves were acquired with r² >0.9999. The contents of liquiritin, glycyrrhizin, and 6-gingerol in YJT were 4.50 mg/g, 11.10 mg/g, and 1.33 mg/g, respectively. YJT inhibited production of TARC and RANTES in TNF-${\alpha}$ and IFN-${\gamma}$-treated HaCaT cells. YJT also reduced production of IL-4, 5, and 13 in primary mouse splenocytes. In conclusion, our data will be a valuable information to improve quality control and anti-allergic effects of YJT.

• Toxicological Research
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• 제29권3호
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• pp.203-209
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• 2013

A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of ${\varepsilon}$-acetamidocaproic acid (AACA), the primary metabolite of zinc acexamate (ZAC), in rat plasma by using normetanephrine as an internal standard. Sample preparation involved protein precipitation using methanol. Separation was achieved on a Gemini-NX $C_{18}$ column ($150mm{\times}2.0mm$, i.d., 3 ${\mu}m$ particle size) using a mixture of 0.1% formic acid-water : acetonitrile (80 : 20, v/v) as the mobile phase at a flow rate of 200 ${\mu}l/min$. Quantification was performed on a triple quadrupole mass spectrometer employing electrospray ionization and operating in multiple reaction monitoring (MRM) and positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of AACA were linear over the concentration range of 20~5000 ng/ml in rat plasma. The coefficient of variation and relative error at four QC levels were ranged from 1.0% to 5.8% and from -8.4% to 6.6%, respectively. The present method was successfully applied for estimating the pharmacokinetic parameters of AACA following intravenous or oral administration of ZAC to rats.

• Ocean and Polar Research
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• 제33권spc3호
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• pp.359-369
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• 2011

This study documents KORDI's experience of successfully deploying a deep ocean buoy for monitoring oceanic and atmospheric variabilities in the tropical western Pacific Ocea nsince May 2010. The primary focus of this study was to compare TRITON (big and old type) with m-TRITON (smaller and new type) buoys within the JAMSTEC's buoy management system. The objective of operating a KORDI buoy is to ascertain oceanic variability in the tropical western Pacific. We adopted a slack-line mooring type to observe water temperatures at six layers from surface to 400 m depth. However, we could not acquire satisfactory results due to lack of expertise in buoy management system. A new KORDI buoy has been developing, which has been modified from both buoys, and ARGOS-3 satellite system and a slack-type mooring line.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 춘계학술대회 논문집
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• pp.264-267
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• 2003

Pitch measurements of 150 nm pitch one-dimensional grating standards were carried out using an contact mode atomic force microscopy(C-AFM) with a high resolution three-axis laser interferometer. It was called as 'Nano-metrological AFM' In Nano-metrological AFM, Three laser interferometers were aligned well to the end of AFM tip. Laser sources of the three-axis laser interferometer in the nano-metrological AFM were calibrated with an I$_2$-stablilzed He-Ne laser at a wavelength of 633 nm. So, the Abbe error was minimized and the result of the pitch measurement using the nano-metrological AFM has a traceability to the length standard directly. The uncertainty in the pitch measurement was estimated in accordance with the Guide to the Expression of Uncertainty in Measurement(GUM). The Primary source of uncertainty in the pitch-measurements was derived from repeatability of pitch-measurement, and its value was approx 0.186 nm. Expanded uncertainty(k=2) of less than 5.23 nm was obtained. It is suggested that the metrological AFM is a useful tool for the nano-metrological standard calibration.

• 센서학회지
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• 제18권2호
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• pp.116-121
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• 2009

Pressure-controlled sodium heat pipe furnace was designed and fabricated, and its characteristics was investigated. Pressure control system was controlled within ${\pm}0.5\;Pa$ at 150 kPa and the stability of pressure was decreased to ${\pm}2.5\;Pa$, when the pressure-controlled system connected with the heat pipe. The melting curve of Ag fixed-point realized well by the adiabatic method using the pressure-controlled sodium heat pipe furnace and its accuracy showed ${\pm}2.27\;mK$ from the calculation of 20% to 80% at the plateau. The freezing curve of Ag fixed-point also realized and its plateau value was 2.23 mK lower than that of the melting curve.