• 약학회지
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• 제60권2호
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• pp.92-99
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• 2016

Parkinson's disease (PD) is one of the representative neurodegenerative movement disorders with the selective loss of dopaminergic neurons in the substantia nigra. 6-Hydroxydopamine (6-OHDA) is widely used as an experimental model system to mimic PD and has been reported to cause neuronal cell death via oxidative and/or nitrosative stress. Therefore, daily intake of dietary or medicinal plants which fortifies cellular antioxidant capacity can exert neuroprotective effects in PD. In the present study, we have investigated the protective effect of Korean red ginseng (KRG) against 6-OHDA-induced nitrosative death in C6 glioma cells. Treatment of C6 cells with 6-OHDA decreased cell viability and increased expression of inducible nitric oxide synthase, production of nitric oxide as well as peroxynitrite, and formation of nitrotyrosine. 6-OHDA led to apoptotic cell death as determined by decreased Bcl-2/Bax, phosphorylation of JNK, activation of caspase-3, and cleavage of PARP. Conversely, pretreatment of C6 cells with KRG attenuated 6-ODHA-induced cytotoxicity, apoptosis, and nitrosative damages. To further elucidate the molecular mechanism of KRG protection against 6-OHDA-induced nitrosative cell death, we have focused on the cellular self-defense molecules against exogenous noxious stimuli. KRG treatment up-regulated heme oxygenase-1 (HO-1), a key antioxidant enzyme essential for cellular defense against oxidative and/or nitrosative stress via activation of Nrf2. Taken together, these findings suggest KRG may have preventive and/or therapeutic potentials for the management of PD.

• 치위생과학회지
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• 제13권3호
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• pp.281-289
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• 2013

본 연구는 구강건강을 중심으로 노인의 삶의 질에 관련된 요인을 찾아내어 향후 노인의 삶의 질을 향상시키기 위한 국가 정책 수립 평가에 기초자료로 제시하고자 한다. 연구 분석 자료는 2007년 7월부터 2009년 12월에 실시한 국민건강영양조사 4기 원시자료로부터 구강검진을 받은 만65세 이상 노인 3,882명의 자료를 활용하였으며 IBM SPSS Statistics 19.0 프로그램을 이용하여 분석하였다. 연구 결과는 잔존치아 수가 많을수록, 국소의치 및 총의치를 필요로 하지 않는 경우 삶의 질이 높았다. 따라서 보철물이 없는 치아 상실이 삶의 질에 크게 작용하므로 잔존치아 수를 증가시키는 노력과 함께 노인에게 치과 보철물 장착을 하여 구강건강을 회복시키는 것이 시급한 과제라고 생각된다.

• 디지털융복합연구
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• 제11권3호
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• pp.317-325
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• 2013

본 연구는 지역사회의 경증인지장애노인과 치매환자에게 인지작업치료를 적용한 후 인지기능과 일상생활활동 수행능력, 우울정도의 효과를 알아봄으로 인지작업치료가 인지기능회복에 미치는 효과를 알아보고자 하였다. 연구방법은 2012년 2월에서 2012년 6월까지 일개광역지역에 소재하는 노인종합복지관과 요양원에 내원한 환자 중 경증인지장애군 13명과 치매군 19명으로 전체 32명을 대상으로 8주 동안 주 1회 신체기능, 인지기능, craft를 이용한 작업치료 활동 프로그램을 실시하였다. 두군 모두에서 인지기능, 우울감에서 유의한 호전을 보였다. 특히 경증인지장애군은 치매보다 집중력과 기억력 유지에 필요한 인지기능에서 유의한 효과를 보여 향후 경증인지장애군에서 인지기능회복을 위한 프로그램의 개발과 예방적 효과에 대한 연구가 필요하다.

• Journal of Ginseng Research
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• 제41권2호
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• pp.188-194
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• 2017

Background: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. Methods: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the $4^{th}$ d. Results: The DPPH radical-scavenging activity of FBG ($IC_{50}=384{\mu}g/mL$) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of $NF-{\kappa}B/p65$, COX-2, and caspase-3 activation. Conclusion: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis.

• 한국식품저장유통학회지
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• 제23권1호
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• pp.74-79
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• 2016

본 연구에서 루꼴라(E. sativa)에 대한 다양한 생리활성을 조사하여 기능성소재 응용가능성을 검토하였다. 루꼴라 추출물은 멜라노마 세포에 대하여 낮은 세포독성을 나타냈다. 세포독성이 거의 없는 농도에서 루꼴라 추출물 처리 시 항산화 활성($ID_{50}$, 17.60 mg/L)이 매우 우수하게 나타났으며 루꼴라 추출물 처리시 높은 tyrosinase 활성저해($ID_{50}$, 132.54 mg/L) 및 멜라닌 함량 저하($ID_{50}$, 158.90 mg/L)를 보여주었다. 또한, 루꼴라 추출물 농도 의존적으로 tyrosinase 발현이 억제되었으며, 이는 루꼴라 추출물이 직접적인 tyrosinase 활성저해 및 세포 내 tyrosinase 발현을 억제시킴으로서 멜라닌 합성을 저해하는 것으로 판단된다. 이와 같은 결과로 미루어 볼 때 루꼴라 추출물은 피부미백 소재 등 피부개선 효과를 지닌 기능성 화장품에 활용하기 위한 매우 효과적인 재료가 될 수 있다고 판단되며 향후 주름개선 효과 등 추가적인 실험을 통하여 화장품 소재 개발 응용 가능성을 타진할 것이다.

• 한방안이비인후피부과학회지
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• 제28권4호
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• pp.51-61
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• 2015

Objective : This research has been conducted to comparative analysis of anti-inflammatory effects to suggest a usefulness of ethanol extracts fromGentianae sino-ornata(GSO) as a functional material for inflammatory activities.Methods : Cell viability was measured by neutral red (NR) assay, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. The expressions of cyclooxigenase-2 (COX-2) was determined by western blot analysis, and Inducible nitric oxide syntase (iNOS) and cytokine were determined by reverse transcriptase-polymerase chain reaction (RT-PCR).Results : When the GSO extract was added the concentration of 5-20 ㎍/㎕, the viability of cells was maintained 90% or more at all levels. NO production was suppressed by the treatment of GSO in LPS-stimulated RAW264.7 cells. GSO inhibited the expression of iNOS, COX-2, IL-1βand IL-6 in LPS-stimulated RAW264.7 cells.Conclusions: From this results, we consider that GSO can be a useful therapeutic and preventive approach to various inflammatory diseases as a functional material for inflammatory activities.

• 대한한의학회지
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• 제19권2호
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• pp.244-270
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• 1998

This study was performed to investigate an anti-HBV activities of the aqueous extracts from 10 Korean herbal medicines in the HepG2 2.2.15 cell culture system and the results were as follows: 1. The extracts of 6 plants (Herba Artemisiae Capillaris, Radix et Rhizoma Rhei, Cortex Cinnamomi, Fructus Chebulae, Fructus Rubi and Radix Rubi) decreased, significantly and dose-dependently, the levels of extracellular HBV virion in the concentrations (10, 100, 500 and $1,000\;{\mu}g/m{\ell}$) tested. 2. However, others (Radix lsatidis, Lignum Sappan, Herba Lysimachiae and Fructus Lycii) did not show any effect either on the replication of HBV or on the levels of virion DNA in the culture media of HepG2 2.2.15 cell. 3. Among the 6 plants which showed the inhibitory potency on the production of extracellular HBV virion, Radix et Rhizoma Rhei, Cortex Cinnamomi, Fructus Chebulae, Fructus Rubi and Radix Rubi except Herba Artemisiae Capillaris also showed the inhibition of the replication of intracellular HEV DNA in the range of $100{\sim}500\;{\mu}g/m{\ell}$. Considering the above results, it is thought that 6 plants(Herba Artemisiae Capillaris, Radix et Rhizoma Rhei, Cortex Cinnamomi, Fructus Chebulae, Fructus Rubi and Radix Rubi) possess the anti-HBV activities in the HepG2 2.2.15 cell culture system. We thus suggest that these plants possess a potential as a therapeutic agent for the chronic viral hepatitis. These results might be useful as a basic data for the development of the new preventive drugs for HBV diseases.

• 대한한의학회지
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• 제29권5호
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• pp.126-133
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• 2008

Purpose : The aim of this study is to identify current situation and issues of outcome measures to evaluate the public health programs using traditional medicine by public health centers in Korea. Method :This study analyse and review existing data and documents related to traditional Korean medicine and health policy using contents analysis method. To collect the information on outcome measures evaluating the programmes, this study reviewed annual reports for health promotion programmes using traditional Korean medicine(HP-TKM) of Hub public health centers, as pilot public health centers, which have implemented the health promotion programmes collectively. Additionally, the review included research articles, government documents and book chapters on the topics related assessments in health promotion. Results :HP-TKM are stroke prevention education, smoke free program, health promotion according to Four Constitutional Medicine, home visiting treatment, etc. However, there are only a few studies of traditional medicine focused health promotion evaluation strategies. The benefits of health promotion programs using TKM can be categorized as non-health benefit, physiological, psychological and physical effects. To manage and monitor the intervention programmes efficiently, attention should be given to developing relevant and valid outcome measures for evaluating the programmes by government and public health center. Conclusion :Up to now, considering number of researchers, research projects undertaken or published articles and reports, within traditional Korean medicine there is a lack of capacity in research. Thus, government should pay more attention to developing relevant and valid outcome measures for evaluating the programs.

• 대한한방소아과학회지
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• 제19권2호
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• pp.215-228
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• 2005

Objective : The study was aimed to evaluate the anti-stress effect of KYT on the cold-stressed Mice. Methods: The experimental animals were stressed in cold room $(4{\pm}0.5^{\circ}C)$ for one hour in a day during two weeks, and administred 131mg/100g KYT extract for two weeks before stress. For two weeks, while the experiment was taking place, we conducted a research about the change of weight and content of norepinephrine, epinephrine, dopamine, cortisol and GOT, GPT in the mice serum. Results : KYT extract significantly inhibited the decrease of body weight induced by stress, compared with the control group. KYT extract had no significant decreasing effect on the change of content of the serum GOT but had significant decreasing effect on the change of content of the serum GPT compared with the control group. KYT extract had significant decreasing effect on the change of content of the serum cortisol compared with the control group. KYT extract had decreasing effect on the change of content of the serum dopamine compared with the control group. KYT extract had no decreasing effect on the change of content of the serum norepinephrine compared with the control group. KYT extract had significant decreasing effect on the change of content of the serum epinephrine compared with the control group. Conclusions : According to the above results, it is concluded that KYT will be useful as a preventive and remedy against stress disease.

• 한국구조물진단유지관리공학회 논문집
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• 제20권1호
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• pp.48-55
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• 2016

구조물의 수명관리는 일반적으로 불확실성의 효율적 고려를 위해 신뢰성 이론을 적용한다. 전체 구조시스템의 신뢰성 평가는 구조요소의 신뢰성 평가와 구조시스템의 모델링을 통해 이루어진다. 구조시스템은 신뢰도에 대한 구조요소의 역할과 기여도를 고려하여 모델링 된다. 따라서, 구조시스템의 신뢰도는 구조요소의 모델링과 구조요소의 상관관계에 따라 서로 다른 결과를 제시하게 된다. 최초 구조시스템의 신뢰도 평가와 열화요소를 반영한 구조시스템의 수명 평가를 바탕으로 생애주기 비용 최소화와 관련된 목적함수를 가지는 최적화 과정을 통해 수명관리가 이루어진다. 본 논문에서는 구조시스템을 구성하는 구조요소의 상관관계와 더불어 기존의 연구에서 고려된 바 없는 유지보수 간의 상관관계에 따른 수명평가 영향분석을 수행하며, 이를 통해 향후 좀 더 효율적인 수명관리 기법 개발에 활용하고자 한다. 또한, 예방유지보수와 필수유지보수를 모두 고려하며, 유지보수간 독립 상태와 완전상관 상태에 따른 수명 예측 및 최적화 유지보수 계획 수립을 비교 제시한다.