• Nutrition Research and Practice
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• v.17 no.4
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• pp.670-681
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• 2023

BACKGROUND/OBJECTIVES: Oxidative stress is caused by reactive oxygen species and free radicals that accelerate inflammatory responses and exacerbate fatigue. Tormentic acid (TA) has antioxidant and anti-inflammatory properties. Thus, the aim of present study is to determine the fatigue-regulatory effects of TA in H₂O₂-stimulated myoblast cell line, C2C12 cells and treadmill stress test (TST) and forced swimming test (FST) animal models. MATERIALS/METHODS: In the in vitro study, C2C12 cells were pretreated with TA before stimulation with H₂O₂. Then, malondialdehyde (MDA), lactate dehydrogenase (LDH), creatine kinase (CK) activity, tumor necrosis factor (TNF)-α, interleukin (IL)-6, superoxide dismutase (SOD), catalase (CAT), glycogen, and cell viability were analyzed. In the in vivo study, the ICR male mice were administered TA or distilled water orally daily for 28 days. FST and TST were then performed on the last day. In addition, biochemical analysis of the serum, muscle, and liver was performed. RESULTS: TA dose-dependently alleviated the levels of MDA, LDH, CK activity, TNF-α, and IL-6 in H₂O₂-stimulated C2C12 cells without affecting the cytotoxicity. TA increased the SOD and CAT activities and the glycogen levels in H₂O₂-stimulated C2C12 cells. In TST and FST animal models, TA decreased the FST immobility time significantly while increasing the TST exhaustion time without weight fluctuations. The in vivo studies showed that the levels of SOD, CAT, citrate synthase, glycogen, and free fatty acid were increased by TA administration, whereas TA significantly reduced the levels of glucose, MDA, LDH, lactate, CK, inflammatory cytokines, alanine transaminase, aspartate transaminase, blood urea nitrogen, and cortisol compared to the control group. CONCLUSIONS: TA improves fatigue by modulating oxidative stress and energy metabolism in C2C12 cells and animal models. Therefore, we suggest that TA can be a powerful substance in healthy functional foods and therapeutics to improve fatigue.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.2
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• pp.287-292
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• 2018

Objective: The aim of present study was to assess the anti-oxidant potential of water-soluble peptides (WSPs) extract derived from buffalo and cow milk Cheddar cheeses at different stages of ripening. Methods: The antioxidant potential of WSPs extract was assessed through 2,2'-azinobis-3-ethylbenzothiazoline-6sulfonic acid (ABTS)-radical scavenging activity. In addition, impact of WSPs extract on cell viability and production of reactive oxygen species (ROS) in human colon adenocarcinoma Caco-2 (tert-butylhydroperoxide-induced) cell lines was also evaluated. Results: The ABTS-radical scavenging activity increased progressively with ripening period and dose-dependently in both cheeses. However, peptide extract from buffalo milk Cheddar cheese demonstrated relatively higher activity due to higher contents of water-soluble nitrogen. Intracellular ROS production in Caco-2 cells decreased significantly (p<0.05) till 150th day of cheese ripening and remained constant thereafter. Additionally, dose-dependent response of WSPs extract on antioxidant activity was noticed in the Caco-2 cell line. Conclusion: On the basis of current in vitro study, the Cheddar cheese WSPs extract can protect intestinal epithelium against oxidative stress due to their antioxidant activity.

• Korean Journal of Psychosomatic Medicine
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• v.4 no.1
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• pp.44-53
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• 1996

The present experiment was designed to investigate the effects of behavioral, response to immune function in response to electric footshock in mice. Mice were subjected to electric footshock for 3 days(two sessions a day, 11 times of shock for about 31 minutes a session). The humoral immune response was measured using mice immunized with rat RBC. The cell-mediated immune responses were evaluated by contact hypersensitivity to 2, 4-dinitrofluorobenzene(DNFB) and by phytohemagglutin(PHA)-stimulated splenocytes proliferation assay. In stressed group, electric footshock suppressed significantly anti-rat RBC antibody production(p<0.05), but enhanced significantly $T_{48}$ relative to $T_{24}$ in contact hypersenstivry (P<.01) and T-cell proliferation response(P<.05) by PHA stimulation elative to control group. T-cell proliferation response by PHA stimulation was significantly correlated to the movement than the sensitivity and coping behavior in the mouse, in response to the electric footshock. These data supper the importance of behavioral response in stress-induced changes of immune functions.

• Natural Product Sciences
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• v.13 no.1
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• pp.78-84
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• 2007

Schizandra chinensis fruits (SC) have been used as a traditional Oriental medicine for treatments of many stress-induced diseases. In the present study, we investigated the protective effect of SC aqueous extract(SC-Ex) in the inflammatory diseases of intestine using a mouse model of ulcerative colitis. An experimental colitis was induced by daily treatment with 5% dextran sulfate sodium (DSS). SC-Ex was orally administered from day 2 of DSS treatment in a dose-dependent manner. Administration of SC-Ex reduced significantly clinic signs of DSS-induced colitis, including body weight loss, shorten colon length, increased disease activity index, and histological colon injury. Moreover, SC-Ex suppressed significantly not only the activities of myeloperoxidase (MPO) and chymase, but also the expressions of $TNF-{\acute{a}}$ and COX-2 in DSS-treated colon tissues. Inhibitory effect of SC-Ex was effective at a dose over 20 mg/kg. Our results indicate that SC-Ex may possess therapeutic effect on the development of DSS-induced colitis.

• Research in Community and Public Health Nursing
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• v.28 no.1
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• pp.98-106
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• 2017

Purpose: To understand the meaning of the lived experience with Person under train (PUT) for train or subway operators. Methods: The study was built on hermeneutic phenomenological themes from individual interviews of present-day train or subway operators in South Korea. Eight participants were selected to participate in the study. All qualitative data were analyzed using the heuristic guides of Van Manen. Results: Four fundamental lifeworld themes and eight sub themes emerged in the findings. The first theme of spatiality had'the place pressed by the darkness' and 'the train drags me there'. The second theme, corporeality had 'a foreboding fear', and 'debris of death that gets stuck in the whole body'. The temporality theme had'distorted time in chaos', and 'memory trapped in time'. Finally the last existential theme of relationality had 'intrapersonal encounter' and 'resentment and guilt'. Conclusion: The four existential lifeworld themes provided a framework for in-depth investigation of the operator's "lived experience." This leads to clear understanding of effects of PUT experience on related individuals. The findings imply that specific active nursing intervention strategies are necessary in order to treat affected train operators, and to prevent further issues in their work and private life.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.12
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• pp.1776-1783
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• 2002

A population segregating for the naked neck (Na) gene was used to evaluate its effect on fast growing broilers at heat stress. An experimental stock comparable to those of modern broilers was established by backcrossing to colour synthetic male and female lines. Matings between heterozygous (Na/na) males and females produced normally feathered (na/na), heterozygous (Na/na) and homozygous (Na/Na) chicks for the present study. Day old to seven week old coloured broilers of three genotypes viz. normally feathered (na/na), heterozygous naked neck (Na/na) and homozygous naked neck (Na/Na) were compared for heat dissipation, growth performance, body conformation traits, blood biochemical parameters and carcass traits in tropical climate. In hot climate, naked neck broilers had significantly less body temperature and better heat dissipation capabilities as compared to normal broilers. The naked neck broilers had significantly higher body weight and better feed conversion ratio than na/na broilers. The Na/Na or Na/na broilers exhibited higher giblet yield, blood loss and lower feather mass compared to na/na broilers. The results indicated that the reduction in feather coverage in Na/Na and Na/na broilers facilitates better heat dissipation with lower body temperature, more body weight gain, better FCR and carcass traits compared to normal broilers.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1399-1403
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• 2009

The aim of the present study was to evaluate the possibility of protective effectness of swimming exercise and Gastrodia elata blume oral administration against beta-cell damage in streptozotocin (STZ)-induced diabetes in rats. The animals were divided into five groups: the normal group(n=10), the STZ-induced diabetes group(n=10), the STZ-induced diabetes and moderate-intensity exercise group(n=10), the STZ-induced diabetes Gastrodia elata blume(300 mg/kg) oral administration group(n=10), the STZ-induced diabetes and moderate-intensity exercise and Gastrodia elata blume(300 mg/kg) oral administration group(n=10). Animals in the exercise groups were made to swim moderate swimming exercise protocols once a day for 4 consecutive weeks. Serum glucose concentration and insulin level, superoxide dismutase (SOD) and catalase (CAT) were measured in serum. Swimming exercise and Gastrodia elata blume extract administration has shown anti-diabetic effect probably through decreasing serum glucose and insulin level and increasing antioxidant enzyme activity.

• Research in Community and Public Health Nursing
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• v.29 no.4
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• pp.476-487
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• 2018

Purpose: The present study is focused on understanding weight perception related with individual- and school-level multifactorial origins, underestimated and overestimated respectively, in normal body weight adolescents. Methods: Using the 2017 Korea Youth Risk Behavior Web-based Survey data of 45,902 students from 799 secondary schools, a multilevel multinomial logistic regression analysis was performed where adolescents (level1) were nested within schools (level 2). Results: At the school level, the average school body mass index (BMI, $kg/m^2$) and physical education were associated with weight perception among both boys and girls. In boys, geographic areas were associated with weight underestimation. At the individual level, perceived economic status, weekly allowance, BMI ($kg/m^2$), smartphone usage time (hrs/day) and perceived stress were associated with weight perception among both boys and girls. Age, paternal education, academic achievement and alcohol use were associated with weight perception among girls, while part-time job and physical activity were associated with weight perception among boys. Conclusion: Our findings underscore the importance of individual- and school-level environments in developing correct weight perception and have implications for school health education to establish healthy lifestyle behaviors for all adolescents.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.265-265
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• 2022

Increased temperature caused by global warming has become a significant problem for the growth and production of crops. A high temperature has a direct or an indirect effect on crops, leading to a significant yield loss. The damage of a high temperature stress to rice depends on its developmental stage. In present study, we performed evaluate the heading date in different location, Yeoju and Miryang, during growth of Korean rice core set. The heading date for the 223 rice accession were evaluated in Yeoju City (37°23' 127°57') and Miryang City (35°50', 128°72') located on middle and southern part of Korea, respectively. The average temperature of a day was higher in Miryang during entire growth stage. Here, total 222 KRICE-Core set was analyzed by GWAS for the high temperature effect. GWAS results revealed the Chr07_26954556, a lead SNPs were significantly associated with delaying heading date of KRICE-Core set. Significance threshold was set with 6.0 > -log10(P), and Cross-Validation (CV) error suggested an optimal K value of 5 for the population based on the lowest cross-validation error K = 5.

• Structural Engineering and Mechanics
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• v.91 no.4
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• pp.417-426
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• 2024

Nanotechnology has emerged as a promising avenue for enhancing musical structures. In this study, we analyze the static behavior of laser harp (i.e., electronic musical instrument) reinforced with Zinc Oxide (ZnO) nanoparticles. Leveraging the piezoelectric properties of ZnO nanoparticles, the structure is subjected to an electric field for intelligent control. The electronic musical structure is situated in a foundation with vertical springs and shear modulus constants. We employ the exponential Shear Deformation Beam Theory (ESDBT) to mathematically model the structure. A micro-electro-mechanical model is employed to determine the equivalent properties of the system. By utilizing nonlinear stress-strain relations, energy methods, and Hamilton's principle, we derive the motion equations. The buckling load of the electronic musical beam is calculated using the Difference Quadrature Method (DQM). The primary objective of this study is to present a mathematical model for electronic musical beams and determining the buckling load of the structure and to investigate the influence of nanotechnology and electric fields on its buckling behavior. The buckling is the case when the structure becomes deforms and unstable. Our findings reveal that the application of negative external voltage to the electronic musical structure increases both the stiffness and the buckling load of the musical system. Furthermore, reinforcing the electronic musical structure with ZnO nanoparticles results in an increased buckling load. Notably, the maximum enhancement in the 28-day compressive and tensile strengths of samples containing zinc oxide nanoparticles compared to the control sample resulting in increases of 18.70% and 3.77%, respectively.