• Title/Summary/Keyword: preparation method

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Study of the oriental medical literature for traditional childbirth (전통적 출산법에 대한 문헌적 고찰)

  • Youn, Seong-min;Kang, Han-ju;Jeong, Woo-seok;Jang, Myeong-jun
    • Journal of Korean Medical Ki-Gong Academy
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    • v.11 no.1
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    • pp.117-143
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    • 2009
  • The community of women into society in various fields is expanding, and married late, and accordingly, the first child birth is increasing age. Accordingly, dystocia has also increased. Listed in the literature that the traditional method of birth seemed to oriental medical interpretation of one of the ancient wisdom of the predecessors to explore. The result of this study is summarized as follows. 1. Child birth custom divide into childbirth preparation, childbirth and postpartum care. Postpartum care divide into preparation of the birthplace, food for pregnant woman and birth preparation of the necessary things. 2. Preparation of the birthplace of the court case and that qeen is three months, one month before that in the case of concubines were installed. The difference in manners, and actual number but the configuration of the same item was prepared. In the private, birthplace is installed husband's home or parent's home. 3. Rice and seaweed is food for pregnant woman. San Miguel (产 米), sangwak (山 藿) specifically called was selected by Keep elaborate. 4. 1 month before birth to baby clothing is usually prepared. For the safe of a paturient woman and a baby, magical prescription was practiced in so-guk-so(昭格署) in early chosun dynasty. After so-guk-so(昭格署) abolished magical practice was stopped. Then Wore old clothes based on dong-ui-bo-gam(東醫寶鑑) 5. Im-san-ye-zi-bub(臨产豫智法) specifically to instruct the royal birth is the birth of guidelines. Im-san-ye-zi-bub(臨产豫智法) cosist of birth place, abdominal pain Precautions, food, care after childbirth, remove the plecenta posion, method of cut navel, bath, protection. Through various court records of the situation, according to the guidelines are properly applied based on dong-ui-bo-gam(東醫寶鑑).

Comparative Studies on Immobilized Invertase on Sepharose and Phenoxyacetyl Cellulose (Sepharose와 Phenoxyacetyl Cellulose에 고정화 시킨 Invertase에 관한 비교 연구)

  • Choi, Choon-Soon;Jeon, Moon-Jin;Byun, Si-Myung
    • Korean Journal of Food Science and Technology
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    • v.12 no.3
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    • pp.176-181
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    • 1980
  • Yeast invertase was immobilized on the 2 kinds of matrices : one is an indirectly coupled enzyme to the cyanogen bromide activated Sepharose by using ${\omega}-aminohexyl$ group as an extension arm, and the other is a tightly adsorbed enzyme on the modified hydrophobic cellulose derivative which has a phenoxyacetyl group as a linkage. The enzyme preparation coupled on Sepharose retained 26.0% of the original activity against sucrose as a substrate, while the preparation immobilized on phenoxyacetyl cellulose retained 72.9% . The immobilized invertase preparation on ${\omega}-aminohexyl$ Sepharose showed the optimal pH 4.5, optimal temperature $60^{\circ}C$, activation energy $5,941\;cal/mole{\cdot}deg$ and Km' 22.2 mM against sucrose, while the preparation adsorbed on phenoxyacetyl cellulose showed the optimal pH 4.0, optimal temperature $60^{\circ}C$, activation energy $7,769\;cal/mole{\cdot}deg$ and Km' 69.9 mM.

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Cryo-SEM Methodology of Arabidopsis thaliana Stem Using High-Pressure Freezing (고압동결고정을 이용한 애기장대 줄기의 cryo-SEM 분석법)

  • Choi, Yun-Joung;Lee, Kyung-Hwan;Je, A-Reum;Chae, Hee-Su;Jang, Ji-Hoon;Lee, Eun-Ji;Kweon, Hee-Seok
    • Applied Microscopy
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    • v.42 no.2
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    • pp.111-114
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    • 2012
  • The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

A Study on the Standardization method of Brining Conditions and Storage Day in the Preparation of Traditional chinese whole Cabbage Kimchi (전통적 통배추김치 제조시 최적절임조건 및 저장기간 설정에 관한 연구)

  • Lee, Jong-Mee;Kim, Hee-Jeong
    • Journal of the Korean Society of Food Culture
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    • v.9 no.1
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    • pp.87-93
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    • 1994
  • This study was conducted to present a method that determine the optimum conditions for the preparation of chinese whole cabbage kimchi. After Sensory and chemical characteristics of kimchi with various salt concentration in brine, brining time and storage period at equal saltiness were measured, the optimum conditions for the preparation of chinese whole cabbage kimchi were determined with the use of the response surface methodology. The results are summarized as follows; 1. The more salt concentration in brine, the longer brining time and storage period, resulted in the lower pH and the higher titratable acidity of kimchis. As the salt concentration in brine and brining time increased, kimchi reaches at optimum titratable acidity of it within a shorter time. 2. As the salt concentration in brine and brining time increased, content of succinic acid decreased but that of lactic, acetic, and propionic acid increased. Amount of citric, malic and succinic acid decreased but that of lactic, acetic acid increased, with storage day. 3. Sensory data showed that firmness and green cabbage flavor of kimchi decreased while toughness, carbonic mouthfeel, sourness and staled flavor increased with increased salt concentration in brine, brining time and storage day. As the storage period increased, crispness of kimchi decreased. 4. The optimum conditions for the preparation of chinese whole cabbage kimchi were as follows: Optimum salt concentration in brine, brining time, and storage period were 19.5%, 3 hours and 45 minutes, and 12 hours.

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Cross-sectional TEM Specimen Preparation of GaN-based Thinfilm Materials Using Alumina Dummy Filler (Alumina dummy 충전재를 이용한 GaN 기반 박막재료의 단면 TEM 시편준비)

  • Oh, Sang-Ho;Choi, Joo-Hyoung;Song, Kyung;Jeung, Jong-Man;Kim, Jin-Gyu;Yu, In-Keun;Yoo, Suk-Jae;Kim, Young-Min
    • Applied Microscopy
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    • v.39 no.3
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    • pp.277-281
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    • 2009
  • Practical difficulties for preparing a good crosssectional specimen of GaN-based materials for transmission electron microscopy have arisen due to large difference of mechanical properties between hard ceramic substrate and soft GaN-layered materials. Uneven polishing, sudden cracking, delamination, and selective sputtering during the conventional wedge polishing technique are often encountered as experimental hindrances. The preparation technique based on Strecker's method can be applied to overcome these difficulties, which eventually leads to mechanically stable TEM samples independent of the mechanical properties of materials. The basic idea is to use hard ceramic dummy filler for embedding the sample of interest into the dummy frame. In this study, we applied this technique into preparing cross-sectional TEM specimen of the GaN-based materials with mechanical instability and demonstrated usefulness of this hard dummy filler method in which the possible modifications of the sample of interest during the preparation must be avoidable. In addition, practical precautions during the preparation were discussed.

Preparation of Silica Nanoparticles via Two-Step Process Utilizing Mixed Chlorosilane Residues

  • Su, Yonghong;Xu, Bugang;Cai, Jixiang;Chen, Liang;Huang, Bing
    • Journal of the Korean Ceramic Society
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    • v.55 no.6
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    • pp.562-569
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    • 2018
  • We propose an economic and facile method for the preparation of silica nanoparticles through a two-step process utilizing chlorosilane residues. Mixed chlorosilane residue was alcoholized with absolute ethanol as a first step to form tetraethoxysilane (TEOS). The TEOS was then utilized as a silicon source to synthesize silica nanoparticles in a sol-gel method. The alcoholysis process was designed and optimized utilizing the Taguchi experimental design method and the yield of TEOS was as high as 82.2% under optimal synthetic conditions. Similarly, the Taguchi method was also utilized to study the effects of synthesis factors on the particle size of silica nanoparticles. The results of statistical analysis indicate that the concentration of ammonia has a greater influence on particle size compared to the mass fractions of TEOS and polyethylene glycol (4.6% and 9.7%). The purity of the silica particles synthesized in our experiments is high, but the specific surface area and pore volume are small.

Analysis of Hypoxia-Inducible Factor Stabilizers by a Modified QuEChERS Extraction for Antidoping Analysis

  • Kim, Si Hyun;Lim, Nu Ri;Min, Hophil;Sung, Changmin;Oh, Han Bin;Kim, Ki Hun
    • Mass Spectrometry Letters
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    • v.11 no.4
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    • pp.118-124
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    • 2020
  • An analytical method was developed for hypoxia-inducible factor (HIF) stabilizers based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation and liquid chromatography-high resolution mass spectrometry analysis. HIF stabilizers potentially enhance the performance of athletes, and hence, they have been prohibited. However, the analysis of urinary HIF stabilizers is not easy owing to their unique structure and characteristics. Hence, we developed the QuEChERS preparation technique for a complementary method and optimized the pH, volume of extraction solvent, and number of extractions. We found that double extraction with 1% of formic acid in acetonitrile provided the highest recovery of HIF stabilizers. Moreover, the composition of the mobile phase was also optimized for better separation of molidustat and IOX4. The developed method was validated in terms of its precision, detection limit, matrix effect, and recovery for ISO accreditation. To the best of our knowledge, this is the first demonstration of the application of the QuEChERS method, which is suitable as a complementary analytical method, in antidoping.

Causal effects of college students' satisfaction of major and martial arts education on their career preparation behaviors - A comparative study between Security Service Major and Martial Arts Major - (전공만족과 무도만족이 진로준비행동에 미치는 영향 - 경호전공과 무도전공 중심으로 -)

  • Gwak, Han-Byuong;Min, Wang-Sik;Kim, Young-Hyun;Yang, Young-Mo;Kim, Woo-Jin
    • Korean Security Journal
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    • no.36
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    • pp.57-92
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    • 2013
  • This study was to confirm the mechanism through which the career preparation behaviors are determined by identifying the structural relationship between influential variables on the career preparation behaviors of college students majoring in security service, and to offer proposals for promoting their career preparation behaviors based on the research results. With this aim, the study analyzes the effect of career decision-making autonomy, satisfaction with a major, and satisfaction with martial arts training on career identity, as well as the influential relationship concerning the effect of career identity on career preparation behaviors. For this, the study set as a population college students majoring in security service in the Gyeonggi region. As a subsequent sampling method, the study selected 300 students through purposive sampling. Then, excluding 13 copies of which the answers were insincere, 263 copies were used for data analysis. The detailed results include the following. First, regarding career decision-making autonomy, it was discovered to have a positive (+) effect on career decision-making self-efficacy. Second, whereas satisfaction with a major had no significant effect on career decision-making self-efficacy, it had a negative (-) effect on career preparation behaviors. Third, satisfaction with martial arts training was found to have a positive (+) effect on career decision-making self-efficacy. Fourth, career decision-making self-efficacy had a positive (+) effect on career preparation behaviors. Fifth, career identity had a negative (-) effect on career preparation behaviors.

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