• 한국임상수의학회지
• /
• 제14권2호
• /
• pp.301-307
• /
• 1997

These studies were performed to provide some basic informations for developing an automatic system in dairy farming cattle in order that the farmers may easily and automatically detect the maintenance of pregnancy and the fact of abortion of the pregnant cows and also to find out the diseased animals with fever. As a method of automatical detection of the maintenance of pregnancy or the fact of abortion, weighing the pregnant cows was conducted from one month-pregnancy to the term using a digital balance. From the first to the 3rd month of pregnancy the body weight of dairy cows was slowly increased (less than 2% per month), then, relatively high increase (3.4% -4.3% per month) from the fourth to the seventh month followed by decrease (3.3%) in the 8th month and very low increase (0.8-0.9%) from the 9th month to the term were shown, resulting in increase of 128.8 kg (25.05%) of body weight to be compared with the first weight. More than 107, increase of body weight to be compared with the first month-weight was denoted from the 61th month of pregnancy and more than 20% increase from the 7th or the 8th month of pregnancy as wells consequently it was presumed that detection of the maintenance of pregnancy is possible from the 4th or the 5th month of pregnancy. It was possible to diagnose a cow aborted at the 6th month by continual weighing the cow from the 1st month of pregnancy. The calved cows showed considerably higher decrease of body weight even in the third week after parturition (p<0.01)to be compared with the body weight near to the term (81.8-102.0 kg, 14-16% decrease). During the same period of 8months, the pregnant cows gained 127.4 kg (24.78% increase), whereas the non-pregnant cows gained 33.0 kg (0.71% increase) to be compared with the first weight showing considerably higher increase of body weight gain in the pregnant cows than the non-pregnant cows (p<0.01). The statistics of body temperatures of dairy cattle were collected from three clinics including the Teaching Hospital of Chonbuk University and the diseases were classified simply by the major symptoms manifested, denoting the highest temperature in respiratory disease ($39.8{\circ}C$) and the lowest in alimentary disease ($39.6{\circ} C$). These informations of body temperatures were expected to be of value for early and automatical detection of the diseased animals with fever when automatic machinery would be established. The results of periodic weighing the body weight of pregnant cows while milking were also expected to be of great use for the farmers to detect the maintenance of pregnancy and the fact of abortion when the automatic system is established in the near future.

• BMB Reports
• /
• 제32권5호
• /
• pp.445-450
• /
• 1999

We purified and characterized a bovine pregnancy-associated protein in pregnant cow urine using two-dimensional gel electrophoresis. Urine from cows was collected according to their status of pregnancy and non-pregnancy. Proteins in the cow urine were fractionated with 50% ammonium sulfate prior to two-dimensional gel electrophoresis. Proteins separated on the gels were compared in terms of expression level and new expression by molecular mass and isoelectric point. We localized two pregnancy-associated protein spots on the gels at molecular masses of 24 kDa and 20 kDa and isoelectric points of 5.5 and 5.7, respectively. Likewise, two non-pregnancy specific proteins were localized at 27 kDa and 28 kDa with isoelectric points of 5.7 and 5.9, respectively. To rule out the possibility that environmental or genetic factors might influence the expression of the proteins, we demonstrated the pregnancy-associated expression of the proteins in two-dimensional gels with pregnant urine taken from cows raised in a different institute. The pregnancy-associated protein with molecular mass of 20 kDa and isoelectric point of 5.7, namely spot 2, was microsequenced and found to be highly homologous to the bovine collagen alpha 1 chain.

• 대한수의학회지
• /
• 제30권2호
• /
• pp.249-253
• /
• 1990

Plasma progesterone concentrations were measured in 24 Korean native cows by a radioimmunoassay at 0, 6 and 20 days after insemination. As a result, 23 cows (95.8%) were judged to be in estrus with ovulation and one cow (4.2%) was judged to be in estrus without ovulation. Plasma progesterone concentrations at 20 days after insemination were $5.11{\pm}1.33$ (mean${\pm}$SD, range 3.20~7.86) ng/ml in pregnant cows and $0.35{\pm}0.10$ (range 0.30~0.50) ng/ml in nonpregnant cows. Of the 20 cows, 18 cows were confirmed the pregnant by rectal palpation at 60 days after insemination. The accuracy of the pregnancy diagnosis based on plasma progesterone concentration was 90 % for positive case and 100% for negative case.

• 대한수의학회지
• /
• 제30권2호
• /
• pp.243-247
• /
• 1990

A study was conducted to improve the reproductive performance of Korean native cows. Plasma progesterone concentrations were determined by radioimmunoassay during the estrous cycle and early pregnancy. Plasma progesterone concentrations during the estrous cycle in the 15 cows were the lowest (0.30ng/ml) at estrus, gradually increased after 6 days, remained high from 8 to 17 days (3.87~5.59ng/ml) and drastically decreased thereafter to reach minimal concentrations at the next estrus. Plasma progesterone concentrations of the 10 pregnant cows at 20 days after insemination were $5.82{\pm}1.33ng/ml$ (mean${\pm}$SD) and those of 10 non-pregnant cows were $0.30{\pm}0.21ng/ml$, respectively. Therefore, plasma progesterone concentrations in the pregnant cows at 20 days after insemination were significantly higher than in non-pregnant cows (p<0.001).

• Journal of Animal Science and Technology
• /
• 제44권5호
• /
• pp.561-572
• /
• 2002

본 연구는 분만전후 40두의 젖소에 무첨가, Aspergillus oryzae(T2), Saccharomyces cerevisiae 혼합제(T3) 및 효소분비촉진제(T4) 급여시 사료섭취량, 산유량 혈중대사물질 및 대사성 질병에 발생에 미치는 영향을 구명하기 위하여 완전임의배치법 10반복으로 수행하였다. 주요 연구결과는 다음과 같다. 분만전 건물섭취량은 처리간에 별 차이가 없었지만 분만일과 분만후 3주 동안에는 Aspergillus oryzae, Saccharomyces cerevisiae 혼합제 및 효소분비촉진제를 급여했을 때가 대조구보다 다소 많았다. 산유량은 사료첨가제를 급여한 구가 무첨가구보다 더 높았으며 특히 효소분비촉진제에서 산유량이 가장 높았다. 혈중 글루코스 및 NEFA 함량은 분만전에는 처리간에 큰 차이가 없었지만 분만일과 분만후 1, 2 및 3일에는 사료첨가제 급여구에서 대조구보다 혈중 글루코스 함량은 더 높고 반면에 NEFA 함량은 더 낮았다. 혈중 Ca 수준은 시험기간동안 처리간에 차이가 없었다. 대사성질병 발생은 대조구에서만 케토시스 1두 발생하였으며, 후산증체는 효소분비 촉진제구는 전혀 발생하지 않았으나 다른 구에서는 공히 2두가 발생하였다. 자궁 내막염은 T2구에서 1두 발생하였다. 결론적으로 전환기 젖소에 사료첨가제를 급여하면 사료섭취량 및 산유량 증가와 더불어 대사성질병 발생이 저하될 것으로 사료된다.

• 한국가축번식학회지
• /
• 제12권1호
• /
• pp.20-23
• /
• 1988

The 3211 Korean native cows raised in Muan, Milyang were investigated for the purpose of finding reproductive rate of cows that retained fetal membrane for two years from April, 1981 to March, 1983. The results obtained were summarized as follows ; 1. The incidence of retained fetal membrane was found in 64 cows(1.98%). 2. The day to first estrus was similar to normal postpartum period unless concurrent genital infection occured. 3. Conception rate of cows that retained fetal membrane was 32.7%, 10.9%, 5.2% and 3.6% at the first, second, third and forth service, respectively and non pregnant cow was 47.6%. 4. Reproductive disorder of non pregnant cows was the highest in repeat breeder and the effect of treatment was very low.

• Animal Bioscience
• /
• 제36권7호
• /
• pp.1130-1142
• /
• 2023

Objective: Cow urine possesses several bioactive properties but the responsible components behind these bioactivities are still far from identified. In our study, we tried to identify the possible components behind the antimicrobial activity of cow urine by exploring the peptidome and metabolome. Methods: We extracted peptides from the urine of Sahiwal cows belonging to three different physiological states viz heifer, lactation, and pregnant, each group consisting of 10 different animals. The peptides were extracted using the solid phase extraction technique followed by further extraction using ethyl acetate. The antimicrobial activity of the aqueous extract was evaluated against different pathogenic strains like Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae. The safety of urinary aqueous extract was evaluated by hemolysis and cytotoxicity assay on the BuMEC cell line. The urinary peptides were further fractionated using high-performance liquid chromatography (HPLC) to identify the fraction(s) containing the antimicrobial activity. The HPLC fractions and ethyl acetate extract were analyzed using nLC-MS/MS for the identification of the peptides and metabolites. Results: A total of three fractions were identified with antimicrobial activity, and nLC-MS/MS analysis of fractions resulted in the identification of 511 sequences. While 46 compounds were identified in the metabolite profiling of organic extract. The urinary aqueous extract showed significant activity against E. coli as compared to S. aureus and S. agalactiae and was relatively safe against mammalian cells. Conclusion: The antimicrobial activity of cow urine is a consequence of the feeding habit. The metabolites of plant origin with several bioactivities are eliminated through urine and are responsible for their antimicrobial nature. Secondly, the plethora of peptides generated from the activity of endogenous proteases on protein shed from different parts of tissues also find their way to urine. Some of these sequences possess antimicrobial activity due to their amino acid composition.

• 한국수정란이식학회지
• /
• 제17권2호
• /
• pp.129-136
• /
• 2002

본 연구는 체내, 체외 소 배반포기 배의 GMP vitrification 후 활력도의 비교와 한우 수정란을 몽골 소에 수정란이식 후 산자생산 가능성을 조사하고자 실시하였다. 한우 수정란은 체외수정란 또는 과배란처리에 의한 체내수정란을 생산하여 GMP vitrification 방법으로 동결 후 몽고로 수송하였다. 수란우는 CIDR과 $PGF_2\alpha$ 처리에 의하여 동기화를 유도하였다 체내수정란생산을 위하여 7두를 과배란처리하였다. 총 64개의 배반포기를 회수하였다. ($9.1\pm2.94$per cow). 체외수정란생산은 80.1% 분할율(174／217)과 40.8% 배반포기 발달율(71／174)을 얻었다. 체내수정란(93.7%; 45／48)의 동결융해 후 생존율은 체외수정란(82.5%; 52／63)보다 유의적으로 높았다(P＜0.05). 8두의 몽골 소에 2개의 수정란을 이식하여 5두가 이식 후 60일째 임신이 확인되었으나, 그 중 1두는 240째 유산을 확인하였다. 그 중 2두의 수란우에서 2두의 산자를 275일째 생산에 성공하였다. 이러한 결과는 GMP vitrification 방법은 체내, 체외수정란의 동결보존방법으로 이용될 수 있을 뿐만 아니라 동결융해란의 몽골 소에 이식 후 한우를 생산할 수 가능성을 확인하였다.

• 한국산학기술학회논문지
• /
• 제16권9호
• /
• pp.6236-6246
• /
• 2015

논문에서는 무인 발정발현 관찰을 통해 다두 사육 시 발생하는 공태를 줄이고, 정확한 수정적기 판단을 통해 번식률을 향상하고자 하였다. 무리 생활을 하는 소의 특성상 운동량의 증가 유무만으로는 발정 발현의 판단이 어렵기 때문에, 소의 운동량 수집 센서와 개체 정보 종합 관리 프로그램을 개발하여 무인 발정발현 관찰시스템을 구축하고 운동량 정보와 번식 정보를 이용하여 발정 발현 관찰 알고리즘을 개발하였다. 시스템과 알고리즘에 대한 성능 검증은 수정 전 시스템을 통해 수정적기를 관찰하고, 수정하고 21일 후 재발정 여부를 확인, 다음 21일 후 초음파 진단을 통해 수태여부를 확인하여 적절하게 수정적기를 판단하였는지 검증하였고, 이를 규모가 유사한 실제 축산 농가 4곳에서 수행하였다. 각 농가의 총 사육 두수는 87, 81, 93, 82두이며 관찰된 발정 예정인 소는 14, 19, 15, 17두 이다. 이중 미약발정에 해당하는 3, 2, 1, 3두는 관찰에 실패하였으나 정상발정에 해당하는 11, 17, 14, 14두의 발정을 감지하였고, 인공 수정 후 10(91%), 17(100%), 13(93%), 14(100%)두의 소가 수태하여 제안된 무인 발정발현 관찰 시스템이 정상발정 시 수정적기 판단에 유효함을 보였다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권4호
• /
• pp.460-466
• /
• 2004

This study was undertaken with the aim to establish a reliable radioimmunoassay (RIA) system for urinary pregnanediol glucuronide (PdG) and to employ it for monitoring the reproductive status of dairy cows. Urine and blood samples were collected from the Holstein cows both pregnant and non-pregnant. The samples were then investigated for evaluating the relationship between progesterone ($P_{4}$) in blood and PdG in urine adjusted with or without urinary creatinine basis. Biweekly urine collection was employed for three cows in estrous and those artificially inseminated, while urine from pregnant cows was collected on a monthly basis. P_{4}$ and PdG levels were measured by enzymeimmunoassay (EIA) and RIA techniques, respectively. Our results indicated the sensitivity of PdG for RIA being 35 pg/tube and the recovery rate of 100%. Urinary creatinine concentrations also fluctuated within a day, but change at midday was not noteworthy. Regardless of the time of urination the change in concentrations of PdG was relatively smaller and did not vary significantly. The urinary PdG concentration showed periodic changes as that with serum P_{4}$ levels during the cow's estrus cycle. The correlation coefficient rose when creatinine level in urine was adjusted but the change was also not significant. The concentrations of PdG during the luteal phase were detected between 8.2 and 17.4 ng/ml, three to five times higher than that in the follicular phase. The concentration of PdG from pregnant cows (21 days after conception) was three to four times higher than in the nonpregnant cows. Our finding suggests that the determination of urinary PdG could be reliably employed for early pregnancy detection. The urinary PdG level continued to raise until 30 days pre-partum while the concentration reached its peak at 30 ng/ml, after which it started to fall 18 to 30 days before parturition and finally fell to its nadir value one week after parturition. As the correlation coefficient between the urinary PdG and serum P_{4}$ was higher than that corrected by urinary creatinine it can be suggested that the adjustment is not needed. The concentrations of urinary PdG could be maintained stably for 2 days in urine samples stored at room temperature and extended to 8 days when the samples were pretreated by boiling for 30 minutes. In conclusion urinary PdG concentration even without the need for creatinine basis adjustment can be used directly for monitoring the reproductive status of dairy cows.