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Purification of Rotavirus Infection-Inhibitory Protein from Bifidobacterium breve K-110

  • Bae, Eun-Ah;Han, Myung-Joo;Song, Mi-Jeong;Kim, Dong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.553-556
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    • 2002
  • The inhibitory activity of fifty Bifidobacteria toward the infectivity of a rotavirus, which is the predominant cause of sporadic diarrhea in infants and young children, was investigated, and Bifidobacterium breve K-110 was found to have the most potent inhibitory activity. Accordingly, the rotavirus infection-inhibitory protein was purified, and its molecular weight was determined to be 76 kDa by SDS-PAGE. It was heat-labile and its 50% inhibitory concentration ($IC_{50}$) was 0.045 $\mu g/ml$.

Indicators of Economic Evaluation and Case Studies on New & Renewable Energy (신재생에너지 경제성 평가 결과 분석 및 평가지표 연구)

  • Ahn Eun-Young;Kim Seong-Yong
    • 한국신재생에너지학회:학술대회논문집
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    • 2005.06a
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    • pp.600-603
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    • 2005
  • U.S. National Research Council proposed benefits framework for energy R&D project as economic benefits, environmental benefits, security benefits and knowledge benefits. Following this framework, U.S. National Renewable Energy Laboratory evaluated the projected benefits of Federal Energy Efficiency and Renewable Energy Programs in the indicators of energy-expenditure savings, energy system cost savings, $CO_2$ emissions reductions. oil savings, natural gas saving and avoided additions to central conventional power. As this result, geothermal energy have predominant position in the energy-expenditure savings, natural gas saving and avoided addi t ions to central conventional power to FY2050. The projected benefits, in monetary value, of the whole supply-potential of geothermal energy in Korea were evaluated as 480.2 billion Won, 43.1 billion Won and 135.8 billion Won for the private energy-cost savings, social environmental-cost savings, and import energy-cost saving, respectively.

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Molecular Mechanism of Inflammatory Signaling and Predominant Role of Saposhnikovia divaricata as Anti-inflammatory Potential

  • Khan, Salman;Kim, Yeong Shik
    • Natural Product Sciences
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    • v.19 no.2
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    • pp.120-126
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    • 2013
  • Natural products have always been a pivotal source of new drug development. Dry roots of Saposhnikovia divaricata (Turcz.) Schischk. (Umbelliferae) is a perennial herb and is also known as Bang Pung in traditional medicine. Numerous in vitro and in vivo studies have revealed the diverse pharmacological effects of S. divaricata and its role in the treatment of various diseases. This herb has exhibited significant inhibitory effects against inflammation and associated disorders. The present study explored the ethnopharmacological applications and molecular mechanisms behind the anti-inflammatory effects of S. divaricata herb and a single compound blockade of multi-signaling inflammatory cascades. Taken together, this review provides insight into the potential role of S. divaricata against various inflammatory diseases.

Difference in Chemotype Composition of Fusarium graminearum Populations Isolated from Durum Wheat in Adjacent Areas Separated by the Apennines in Northern-Central Italy

  • Prodi, A.;Purahong, W.;Tonti, S.;Salomoni, D.;Nipoti, P.;Covarelli, L.;Pisi, A.
    • The Plant Pathology Journal
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    • v.27 no.4
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    • pp.354-359
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    • 2011
  • Chemotype composition of Fusarium graminearum strains, isolated from durum wheat kernels from naturally FHB infected fields in Northern and Central Italy, was investigated by multiplex PCR. The different climatic and environmental conditions of the two examined areas separated by the Apennines affected the composition of chemotypes. 15Ac-DON chemotype was predominant in both the sub areas. Nivalneol chemotype was more frequent in the warmer sub area.

Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis

  • Song, Hyun-Ouk;Ryu, Jae-Sook
    • Parasites, Hosts and Diseases
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    • v.51 no.4
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    • pp.479-484
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    • 2013
  • Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion ($O^{\cdot}_{2^-}$) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis.

A Study on the Characteristics of Sound Source of Hydrodynamic proceeding Bearings (유체 윤활 저널베어링의 음원 특성에 관한 연구)

  • Rho, Byoung-Hoo;Kim, Kyung-Woong
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2002.05a
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    • pp.156-161
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    • 2002
  • Results of theoretical investigations on acoustical properties of hydrodynamic proceeding bearings are presented. Nonlinear analysis of rotor bearing system including rotor imbalance is performed in order to obtain acoustical properties of hydrodynamic proceeding bearings. Furthermore, a cavitation algorithm, implementing the Jakobsson-Floberg-Olsson boundary condition, is adopted to predict cavitation regions in a fluid film. Acoustical properties of hydrodynamic proceeding bearings are identified through frequency analysis of pressure fluctuation calculated from the nonlinear transient analysis. The results show that the acoustical frequency spectra of hydrodynamic proceeding bearings are pure tone spectra, containing the frequency of the shaft rotation and its super-harmonics. The analysis also shows that the super-harmonics are predominant at neighborhood of the fluid film reformation and rupture regions.

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Isolation of Staphylococcus Aureus from the Tonsils of Healthy Pigs (돼지편도로부터 STAPHYLOCOCCUS AUREUS균의 분리)

  • 이재봉;고주영;조영보;정승기
    • Korean Journal of Veterinary Service
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    • v.14 no.2
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    • pp.143-147
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    • 1991
  • Between June and August 1990, the tonsils of 86 healthy pigs were examined for the presence of staphylococci. All of the pigs examined harboured Staphylococci in the tonsils, the most predominant Staphylococcus species was Staphylococcus aureus(45.3%) followed by Staph hyicus subsp chromogenes (20.9%), Staph hyicus subsp hyicus (16.3%), Staph hominis(4.7%), Staph simulans(2.3%) and Staph xylosus(1.2%), Unidentifiable species were isolated from 3(3.5%) of the 86 tonsils examined. Thirty-nine strains of Staph aureus were subjected to the biotyping scheme of Hajek & Marsalek all the strains were classified as biotype B.

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Genetic variations in open reading frame 2 gene of porcine circovirus type 2 isolated in Korea during 2016-2017

  • Kim, Kiju;Choi, Jong-Young;Lyoo, Kwang-Soo;Hahn, Tae-Wook
    • Korean Journal of Veterinary Research
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    • v.58 no.3
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    • pp.143-146
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    • 2018
  • The capsid protein of porcine circovirus type 2 (PCV2) encoded by open reading frame 2 (ORF2) is important for neutralizing activity against PCV2 infection. This study investigated the heterogeneity of the ORF2 gene of PCV2 isolated in Korea during 2016-2017. The results revealed that PCV2d is currently the predominant genotype. Moreover, comparison of ORF2 from 17 PCV2 isolates revealed 88.3-100% homology at the nucleotide (deduced amino acid 86.3-100%) level. Interestingly, 61.5% (8/13) of the PCV2d isolates had glycine at position 210. These data provide a useful information for PCV2 epidemiology in Korea.

Pulmonary Aspergillosis - Pulmonary Aspergillosis - (폐 Aspergillosis -6예 보고-)

  • 김치경
    • Journal of Chest Surgery
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    • v.12 no.1
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    • pp.1-8
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    • 1979
  • Pulmonary aspergillosis is being recognized with increasing frequency in recent years and the-rising incidence of this infection parallels certain medical advances in antibiotics, chemotherapeutic and immunosuppressive therapy. The cavities of lungs resulting from tuberculosis, histoplasmosis or neoplasm are apt, to be infected by one of the species of the genus Aspergillus and eventually mycetomas are formed within the cavities. Authors have experienced 6 cases of pulmonary aspergillosis forming mycetoma in Dept. of Thoracic Surgery, Catholic Medical Center from Aug. 1976 to Feb. 1979. Hemoptysis or blood tinged sputum, the predominant symptom, occurred in all cases. All patients underwent pulmonary resection, 1 pneumonectomy, 3 lobectomies, 1 lobectomy with segmental resection and 1 segmental resection and survived well without death or complication. Primary aspergillosis was in 2 cases and underlying diseases were present in 4 cases: 3 pulmonary tuberculosis, 1 bronchiectasis. The common diagnostic study of intracavitary mycetoma was the posterioanterior chest roentgenogram; in cavities suspected of being diseased or in doubtful cases, tomography was most available to find fungus ball with air-meniscus shadow.

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Double Aortic Arch with Right Sided Descending Aorta - Report of 1 case - (우측 하행 대동맥을 동반한 중복 대동맥궁 - 1례 수술 치험 -)

  • 조경수
    • Journal of Chest Surgery
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    • v.23 no.1
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    • pp.201-204
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    • 1990
  • The double aortic arch is the commonest anomaly among the vascular rings are relatively rare congenital vascular anomalies. This anomaly is malformation of the aortic arch system may, by compression of the trachea and esophagus, cause respiratory distress and dysphagia. We experienced one case of double aortic arch with right sided descending aorta with predominant right anterior arch treated surgically at Kyung Hee University Medical Center. 1-year-old male patient with acute airway obstruction due to combination of double aortic arch and right descending aorta. The diagnosis was made by simple X-ray & confirmed by barium esophagogram & aortogram. The operative approach was through left thoracotomy & underwent division of the left aortic arch & division of ligamentum arteriosum & suspension of divided proximal end of anterior arch to anterior thoracic wall. The postoperative courses was uneventful and doing well on the 3 years.

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