• Title/Summary/Keyword: pre-state

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Choline-Lipid Release from Normal and Transformed Cells

  • Hong, Seong-Tshool;Jang, Yong-Suk;Park, Kie-In
    • BMB Reports
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    • v.29 no.1
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    • pp.73-80
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    • 1996
  • The effect of albumin on phosphatidylcholine (PC) metabolism in Hep-G2, 3T3-H.ras, and 3T3 cells pre-labelled with [Me-$^3H$]choline was studied. The [$^3H$]choline was more efficiently taken up and incorporated into cellular phospholipids in 3T3-H.ras cells than in Hep-G2 and 3T3 cells. In each of the three cell lines, most of the [$^3H$]choline metabolized into the phospholipids was incorporated into PC and only minor was incorporated into lysophosphatidylcholine (LPC). Bovine serum albumin stimulated the release of [$^3H$]LPC and [$^3H$]PC from each of the three cell lines pre-labelled with [$^3H$]choline. [$^3H$]PC was also released in the absence of albumin but [$^3H$]LPC was not. The efficiency of LPC secretion represented as the proportion of medium [$^3H$]LPC to cellular [$^3H$]choline lipid during a chase period is approximately 9 to 14 times greater in 3T3 cells compared with the transformed 3T3-H.ras and Hep-G2 cells. A similar comparison of published data for rat hepatocytes with Hep-G2 shows secretion to be 35~75 times greater from the rat hepatocytes than from Hep-G2. Also, PC secretion from 3T3 cells was 1.6 times more effective than from 3T3-H.ras, whereas rat hepatocytes secrete PC 2.8~3.8 times more effectively than does Hep-G2. The measurement of specific radioactivity of cellular PC in pre-labelled 3T3 cells showed it to be similar to that of the secreted PC. However, the specific radioactivity of secreted LPC was markedly lower than that of the cellular PC, which suggests that LPC is being secreted from a PC pool distinct from that used for PC secretion.

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Development of Pre-Processing and Bias Correction Modules for AMSU-A Satellite Data in the KIAPS Observation Processing System (KIAPS 관측자료 처리시스템에서의 AMSU-A 위성자료 초기 전처리와 편향보정 모듈 개발)

  • Lee, Sihye;Kim, Ju-Hye;Kang, Jeon-Ho;Chun, Hyoung-Wook
    • Atmosphere
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    • v.23 no.4
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    • pp.453-470
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    • 2013
  • As a part of the KIAPS Observation Processing System (KOPS), we have developed the modules of satellite radiance data pre-processing and quality control, which include observation operators to interpolate model state variables into radiances in observation space. AMSU-A (Advanced Microwave Sounding Unit-A) level-1d radiance data have been extracted using the BUFR (Binary Universal Form for the Representation of meteorological data) decoder and a first guess has been calculated with RTTOV (Radiative Transfer for TIROS Operational Vertical Sounder) version 10.2. For initial quality checks, the pixels contaminated by large amounts of cloud liquid water, heavy precipitation, and sea ice have been removed. Channels for assimilation, rejection, or monitoring have been respectively selected for different surface types since the errors from the skin temperature are caused by inaccurate surface emissivity. Correcting the bias caused by errors in the instruments and radiative transfer model is crucial in radiance data pre-processing. We have developed bias correction modules in two steps based on 30-day innovation statistics (observed radiance minus background; O-B). The scan bias correction has been calculated individually for each channel, satellite, and scan position. Then a multiple linear regression of the scan-bias-corrected innovations with several predictors has been employed to correct the airmass bias.

Effect of Skin Cancer Training Provided to Maritime High School Students on Their Knowledge and Behaviour

  • Sumen, Adem;Oncel, Selma
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.17
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    • pp.7769-7779
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    • 2015
  • Background: This study was conducted with the purpose of evaluating the effect of skin cancer training provided to maritime high school students on their knowledge and behaviour. Materials and Methods: The study had a quasi-experimental design with pre-test and post-test intervention and control groups. Two maritime high schools located in the city of Antalya were included within the scope of the study between March and June 2013, covering a total of 567 students. Results: While the knowledge mean scores of students regarding skin cancer and sun protection did not vary in the pre-test ($6.2{\pm}1.9$) and post-test ($6.8{\pm}1.9$) control group, the knowledge mean scores of students in the experimental group increased from $6.0{\pm}2.3$ to $10.6{\pm}1.2$ after the provided training. Some 25.4% of students in the experimental group had low knowledge level and 62.2% had medium knowledge level in the pre-test; whereas no students had low knowledge level and 94.3% had high knowledge level in the post-test. It was determined that tenth grade students, those who had previous knowledge on the subject, who considered themselves to be protecting from the sun better, had higher knowledge levels and their knowledge levels increased as the risk level increased. It was found that the provided training was effective and increased positively the knowledge, attitude and behaviour levels of students in the experimental group in terms of skin cancer and sun protection. Conclusions: Along with the provided training which started to form a lifestyle, appropriate attitudes and behaviours concerning skin cancer and sun protection could be brought to students who will work in outdoor spaces and are members of the maritime profession within the risk group.

On the origin of gas deficient galaxies in galaxy clusters: insights from cosmological hydrodynamic simulations

  • Jung, Seoyoung;Choi, Hoseung;Wong, O. Ivy;Kimm, Taysun;Chung, Aeree;Yi, Sukyoung K.
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.33.2-34
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    • 2018
  • Galaxies associated with massive groups/clusters are normally gas deficient in contrast to field galaxies. HI observations on such galaxies have revealed signs of violent gas stripping, the direct evidence of the environmental effect. At the same time, the notable number of passive galaxies at the cluster outskirts indicates the presence of pre-processing that makes galaxies gas-poor before entering clusters. We investigate the possible channels for the production of the gas deficient galaxies using the state-of-the-art cosmological hydrodynamic zoom-in simulations of 16 clusters (Choi&Yi). We find cluster effect and pre-processing together play an important role in producing the gas-poor galaxies and in both cases gas loss qualitatively agrees with the ram pressure stripping description. Among the currently gas-poor cluster galaxies, 34% are pre-processed before the cluster infall. They are mainly satellites that have undergone ram pressure stripping in group halos. 43% deplete quickly after arriving at cluster during their first approach to the center. Some of them are group halo satellites low in the gas at the infall compared to galaxies directly coming from the field. 24% retain gas even after their first pericentric pass mainly because they are falling into low mass clusters and/or they have a circular orbit that minimizes the ram pressure effect. This study highlights the importance of the past history of galaxies, especially in group halos, before joining the current cluster when understanding the excess of passive galaxies in clusters.

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Planar measurements of OH and $O_{2}$ number density in premixed $C_{3}$H$_{8}$O$_{2}$ flame using laser induced pre-dissociative fluorescence (레이저 유도 선해리 형광법(LIPE)을 이용한 화염내 OH 및 $O_{2}$ 분자의 2차원 농도 분포 측정)

  • Jin, Seong-Ho;Nam, Gi-Jung;Kim, Hoi-San;Chang, Nae-Kak;Park, Seung-Han;Kim, Ung;Park, Kyoung-Suk;Shim, Kyoung-Hoon;Kim, Gyung-Soo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.20 no.12
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    • pp.4044-4052
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    • 1996
  • Planar images of OH and $O_{2}$ with tunable KrF excimer laser which has a) 0.5 $cm^{-1}$ / linewidth, b) 0.5 nm tuning range, c) 150 mJ pulse energy, and d) 20 ns pulse width are obtained to determine spatial distributions of OH and $O_{2}$ in premixed $C_{3}$H$_{8}$ /O$_{2}$ flame. The technique is based on planar laser induced pre-dissociative fluorescence(PLIPF) in which collisional quenching is almost avoided because of the fast pre-dissociation. Dispersed LIPF spectra of OH and $O_{2}$ are also measured in a flame in order to confirm the excitation of single vibronic state of OH and $O_{2}$, OH and $O_{2}$ are excited on the P$_{2}$(8) line of the $A^{2}$.SIGMA.$^{+}$(v'= 3)-X$^{2}$.PI.(v'||'||'&'||'||'quot;= 0) band and R(17) line of the Schumann-Runge band B$^{3}$.SIGMA.$_{u}$ $^{[-10]}$ (v'= 0)- X$^{3}$.SIGMA.$_{g}$ $^{[-10]}$ (v'||'||'&'||'||'quot;= 6), respectively. Dispersed OH and $O_{2}$ spectra show an excellent agreement with simulated spectrum and previous works done by other group respectively. It is confirmed that OH widely distributed around flame front area than $O_{2}$.

Effects of Red Bean (Vigna angularis) Protein Isolates on Rheological Properties of Microbial Transglutaminase Mediated Pork Myofibrillar Protein Gels as Affected by Fractioning and Preheat Treatment

  • Jang, Ho Sik;Lee, Hong Chul;Chin, Koo Bok
    • Food Science of Animal Resources
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    • v.36 no.5
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    • pp.671-678
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    • 2016
  • Fractioning and/or preheating treatment on the rheological properties of myofibrillar protein (MP) gels induced by microbial transglutaminase (MTG) has been reported that they may improve the functional properties. However, the optimum condition was varied depending on the experimental factors. This study was to evaluate the effect of red bean protein isolate (RBPI) on the rheological properties of MP gels mediated by MTG as affected by modifications (fractioning: 7S-globulin of RBPI and/or preheat treatment (pre-heating; 95℃/30 min): pre-heating RBPI or pre-heating/7S-globulin). Cooking yields (CY, %) of MP gels was increased with RBPI (p<0.05), while 7S-globulin decreased the effect of RBPI (p<0.05); however, preheating treatments did not affect the CY (p>0.05). Gel strength of MP was decreased when RBPI or 7S-globulin added, while preheat treatments compensated for the negative effects of those in MP. This effect was entirely reversed by MTG treatment. Although the major band of RBPI disappeared, the preheated 7S globulin band was remained. In scanning electron microscopic (SEM) technique, the appearance of more cross-linked structures were observed when RBPI was prepared with preheating at 95℃ to improve the protein-protein interaction during gel setting of MP mixtures. Thus, the effects of RBPI and 7S-globulin as a substrate, and water and meat binder for MTG-mediated MP gels were confirmed to improve the rheological properties. However, preheat treatment of RBPI should be optimized.

Adult Eclosion and Emergence of the Black Pine Bast Scale, Matsucoccus thunbergianae (Homoptera: Coccoidae: Margarodidae) (솔껍질깍지벌레 성충의 우화습성)

  • ;Law
    • Korean journal of applied entomology
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    • v.30 no.1
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    • pp.86-93
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    • 1991
  • Adult eclosion and emergence behavior of the black pine base scale, Matsucoccus thunbergianae Miller and Park, was studied in a laboratory. Adult eclosion in males did not appear to be limited to a specific time of the day. Newly molted male adults, at room temperatures, usually remained in the cocoons as pre-emergence adults for 0.5-20. days before emerging; most males emerged within one hour after the onset of photophase, and emergence was faster at higher light intensity. Most females molted into adults within three hours after the onset of photophase, and they were active immediately after molting. Quiescence in males appears to help the scales concentrate their sexual activity within a particular period of the day as well as to protect newly molted adults until sexual and locomotive maturity.

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The Relation Between the Chinese Ceclusion Culture and the Gentleman's Gardens (중국 은일문화의 사대부원림의 관계 -선진시대에서 당조까지-)

  • 임상섭;조정송
    • Journal of the Korean Institute of Landscape Architecture
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    • v.26 no.4
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    • pp.113-124
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    • 1999
  • The purpose of this study is to search into the relation between the chinese seclusion culture and the gentleman's gardens by means of the historical and aesthetic approach. The periodic limits of this study is from pre-Qin to Tand. For gentleman's gardens in Tang dynasty at once got out of the noble's gardens which had imitated the imperial gardens until Tang, and became the origin of the gardens of the literati appearing in post-Tang dynasty. The major findings are as follows : First, in pre-Qin period, the origin of the seclusion culture is classified as the metaphysical seclusion of Taoism and the physical seclusion of Confucianism. Although Pu, Yuan, and Uou which could be said the first step of the chinese gardens were inquired, the clear relation between the seclusion and them is not found. Second, during Qin-Han dynasty, the role of the gardens, as the settings of the seclusion, are comparatively minor, too. But uan Kuang-Han's garden considerably escaped from that of pre-Qin in the aspect of function, and it has the characteristics of recreation. Third, during Wei-Chin and Liu dynasty, two types of the seclusions emerged. One is the small seclusion, and the other the Great Seclusion. Though, as formerly, the contemporary gardens did not escaped from the imperial gardens, Shi Chong's garden can be the setting of the Court Seclusion. But the seven Righteous in Bamboos' and Tao Yuan-Ming's settings of the seclusion were real natural environments rather than gardens. Forth, in Tang dynasty, in spite of relatively low status, Bai Ju-Yi's theory of the middle Seclusion enabled gentlemen to make seclusion in sinecures unlike the court Seclusion. The theoretical background of the Middle Seclusion was Zen Buddhism and the State Examinations System and Bai Ju-Yi's private social position also had an important effect on it. In Tang dynasty, there being nobiliary gentleman's gardens, Bai Ju-Yi's garden, Lushan Caotangyuan, was the setting of the Middle Seclusion. It can be called a real gentleman's garden, for it is simple and small unlike the imperial gardens or the nobiliary gentleman's gardens.

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Redistributions of Welding Residual Stress for CTOD Specimen by Local Compression (Local compression에 의한 CTOD 시편내의 용접잔류응력 재분포)

  • Joo, Sung-Min;Yoon, Byung-Hyun;Chang, Woong-Seong;Bang, Han-Sur;Bang, Hee-Seon;Ro, Chan-Seung
    • Journal of Welding and Joining
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    • v.27 no.6
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    • pp.31-35
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    • 2009
  • When conducting CTOD test, especially in thick welded steel plate, fatigue pre-cracking occasionally failed to satisfy the requirements of standards thus making the test result invalid. Internally accumulated residual stress of test piece has been thought as one of the main reasons. The propagation of fatigue crack, started from the tip of machined notch, which might have propagated irregularly due to residual stress field. To overcome this kind of difficulty three methods to modify the residual stress are suggested in standard i.e. local compression, reverse bending and stepwise high-R ratio method. In this paper not only multi pass welding but also local pre-compressing process of thick steel plate has been simulated using finite element method for clarifying variation of internal welding residual stress. The simulated results show that welding residual stress is compressive in the middle section of the model and it is predominantly increased after machining the specimen. Comparing as-welded state all component of the welding residual stress changing to compressive in the tip of machine notch whereas residual stress of the outer area remain as tensile condition relatively. Analysis results also show that this irregular residual stress distribution is improved to be more uniformly by applying local compression.

Application and Development Trend of OTDRs (분포형 광섬유센서의 응용 및 개발 동향)

  • Chae, Kwagn-Seok;Lee, Sang-Pil;Lee, Chang-Ho;Han, Sung-Jae
    • Tunnel and Underground Space
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    • v.21 no.1
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    • pp.1-10
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    • 2011
  • In these days, the development of optical fiber sensor technology is so remarkable that it can measure various physical and chemical quantities ranging from a few millimeters to over several kilometers. In addition, it is attempted to assess the structural integrity of the state of the advanced technologies and existing structures such as ships, aircrafts, and bridges. This paper introduced the case histories of the measuring technology of optical fiber applied on structures such as roads and tunnels. The case history using OTDR (Optical Time Domain Reflectometery) was also introduced in this paper. Measurement of the pre-convergence of a tunnel is essential to assess the safety of a tunnel and understand the geological conditions ahead of an advancing tunnel. Therefore, the pre-convergence measuring technology using OTDR is expected to substitute conventional measuring techniques.