• 한국축산식품학회지
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• 제42권6호
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• pp.942-952
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• 2022

To establish a pre-plating method of chicken satellite cells with high purity, pre-plating was performed under culture conditions of 37℃ and 41℃, and the pre-plating time was set from a total of 3 hours to 6 hours in consideration of the cell attachment time. The purity of the cells was confirmed by staining paired box protein 7 (Pax7) after proliferation, and Pax7 expression was the highest in culture flasks shaken for 2 hours after incubation at 41℃ for 2 hours to prevent the attachment of satellite cells (p<0.05). Also, when pre-plating and proliferation were performed at 37℃ and 41℃, the Pax7 expression rate was higher at 41℃. The differentiation capabilities of the three groups (T3, T6, and T7) with high Pax7 expression were compared and the fusion index (%) and myotube formation area (%) determined by myosin heavy chain (MHC) staining was calculated. The T6 and T7 groups, which were cultured at 41℃, showed significantly higher values than the T3 group (p<0.05). There was no significant difference in the expression of Pax7 and MHC between the T6 and T7 groups (p>0.05). These results suggest that pre-plating at 41℃ for a total of 4 hours was the most efficient in terms of cost and time for purifying chicken satellite cells for cultured meat.

• 영미문화
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• 제14권1호
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• pp.269-286
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• 2014

The Pre-Raphaelite oeuvre abounds in the image of women, which indicates the impact of gender question on contemporary visual culture. The representation of women in their art tends to evince the entrenched myth of womanhood, marked by a stereotyped dichotomy in the apprehension of femininity. Yet there are a significant number of pictures which attest to the point that their iconography of womanhood cannot be fully elucidated by exploring the dichotomy alone. They falsify the dyadic model, defying the attempt to accommodate them in a clean-cut category. The curious blend of the mystical, the sensual, and the domestic that characterizes these images suggests that they are open to multiple interpretations. In sum, the Pre-Raphaelite representation of women both endorses and challenges the ideal of femininity, indicating that it was shaped by and shaped contemporary perceptions of women at a time when gender relations were shifting and the traditional institution of patriarchy revealed a sign of strain.

• International Journal of Advanced Culture Technology
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• 제10권4호
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• pp.23-244
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• 2022

The Pulpose of this systematic review is aimed to establish the procedure of the injection with saftey and efficiency in the pre-hospital cardiac arrest patient performing the cardiopulmonary resuscitation (CPR), compared with traditional medication administration using Ampoule and medication administration with Prefilled Syringe. Databases were searched for CPR, heart arrest, resuscitation, Pre-filled Syringe, and Ampoule by the electronic data research including Pubmed, EMBASE and Cochran Library of Konyang University Library: 4 articles were selected by three co-authors using EndNote X20 and Covidence (Covidence.org) and were systematically reviewed. The Result of this study, the medication administration using Pre-fillled Syringe improves the safety of patients and Emergency medical workers by reducing the error in administration dose and administering the drug in safe than the medication adminisrtaion using Ampoule, also, contributes to the increment of survival rate of cardiac arrest and severe patients by decreasing the administration time that prevents the delay of medication administration.

• 복식문화연구
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• 제19권2호
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• pp.324-333
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• 2011

This study was accomplished for the purpose of developing a textile processing ingredient that is harmless to the human body and environment. The research method consists of dyeing cotton textiles by extracting the dye solution from ginseng. Then, chrominance, after treatment, antibacterial ratio and deodorization ratio of cotton fabrics dyed with ginseng extracts were tested and results were examined. The research procedure involved first extracting the dye solution from the ginseng's by-product (fine roots) and then dyeing was effectuated differently according to the test samples temperature and dyeing time requirements. Brightness in all dye substances was lower in pre-mordanting. Beige color could be extracted from pre-mordanted samples. And dark orange from postmordanted samples. Color-festness was high in all samples. Most of samples show a big antibacterial ratio and deodorization ratio. Through this research it has been discovered that, when applied to textiles, Korea's ginseng extract possessed reproducibility features as a natural dye and a possibility to be used in cutting which plays a crucial role in hygienic processing. In addition, by using ginseng's by-product for dyeing processing as the dye solution, efficient application of resources and occurrences of no water waste damages were demonstrated and thus, proved to be environmentally-friendly. Specifically, through this experiment, it was found that saponin, ginseng's special characteristics, possessed excellent antibacterial odor repelling functions to clothing as well as the capability to prevent skin disease.

• 한국정밀공학회지
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• 제27권2호
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• pp.153-159
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• 2010

Recently, it has been reported that mechanical stimulation takes a role in improving cell growth. Also, became generally known that skeletal system as bone or cartilage tissues take influence of compression loading. In this study, we fabricated a custom-made bioreactor and analyzed that conditions of compressive loading would influence cell growth. To compare the effect of intermittent compressive loading on cell-encapsulated agarose scaffold, we cultured preosteoblast cell (MC3T3-E1 cells) statically and dynamically. And dynamic culture conditions were produced by changing parameters such as the iteration time and interval delay time. Also, cellencapsulated agarose scaffold were subjected to 10 % dynamic compressive strain at 1㎐ frequency for 7 days. After cell culture, cell proliferation was assessed with PI stain assay for fluorescence images and flow cytometry (FACS).

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.187-193
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• 2009

The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for long periods of time in culture. The colonies displayed morphology similar to miceSSC and were positive for markers of SSC (PGP9.5), proliferating germ cell (PigVASA), pre-meiotic germ cell (DAZL) and pluripotency (OCT4, SSEA-1, NANOG, and SOX2) based on immuno-cytochemistry and RT-PCR. The purity of these colonies was confirmed by negative expression of markers for sertoli cell (GATA4 and SOX9), peritubular myoid cell (${\alpha}$-SMA), differentiating spermatogonial and germ cells (c-KIT). The colonies could be maintained with undifferentiated morphology for more than two months and passaged more than 8 times with doubling time between 6-7 days. Taken together, we conclude that pigSSC could be successfully isolated and cultured in vitro and they possess characteristics similar to miceSSC.

• 가족자원경영과 정책
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• 제10권1호
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• pp.17-32
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• 2006

This essay has attempted to document the actual behavior patterns and the social networks related to the child delivery in the pre-industrial Korean society. We interviewed 30 women who had given birth to their first child during the 1930s and the 1940s in order to accumulate data related to son-prayer rites, prenatal care and food avoidance, sacred-string culture, and other incantation rituals. The characteristics of the social relationships with the person who had assisted the delivery and the recovery were also analyzed in terms of kinship networks. The results are as follows. First, there was a big gap between knowledge and the actual practices in birth rituals and customs. We assume that this is due to the adverse social-economic conditions at that time which may have restricted the actual performances of these customs. Second, there were almost no differences of the performance of these' birth customs between the urban areas and the rural areas. Third, the people who assisted the delivery were women who were mostly from the husband's family. Help from the wife's family were quite exceptional. Finally, it has been found out that only about half of the women who were interviewed performed the well-known custom of three-week after-birth confinement.

• International Journal of Advanced Culture Technology
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• 제6권3호
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• pp.142-150
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• 2018

This paper describes the algorithm that lowers the dimension, maintains the object recognition and significantly reduces the eigenspace configuration time by combining the edge orientation histogram and principle component analysis. By using the detected object region as a recognition input image, in this paper the object recognition method combined with principle component analysis and the multi-layer network which is one of the intelligent classification was suggested and its performance was evaluated. As a pre-processing algorithm of input object image, this method computes the eigenspace through principle component analysis and expresses the training images with it as a fundamental vector. Each image takes the set of weights for the fundamental vector as a feature vector and it reduces the dimension of image at the same time, and then the object recognition is performed by inputting the multi-layer neural network.

• International Journal of Advanced Culture Technology
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• 제9권3호
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• pp.232-237
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• 2021

Pre-Corona cultural content was largely formed face to face. For example, the service was great while visiting tourist attractions, visiting performances to see K-Pop, or visiting lectures to learn K-Beauty. However, after the coronation, the virtual world-centered Metaverse began to take the spotlight as non-face-to-face services changed. IT and cultural contents have been combined and developed into various digital services. In that sense, Metaverse, which breaks the boundaries between real-world space and virtual space, has the potential to escape from physical time and space constraints. However, Korea still lacks a Metaverse platform for cultural content. In particular, it is necessary to match each other's necessary services by providing Metaverse -oriented cultural contents that users want, including experienced cultural contents such as K-pop and K-beauty, away from cultural contents that are developed around history museums. Therefore, in this paper, we would like to propose a Metaverse-based cultural content matching platform where users can experience cultural content directly or indirectly.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 제9차 국제심포지움 및 추계정기학술발표회
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• pp.53-53
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• 2002

Shoot tips of in vitro propagated plantlets were cryopreserved using encapsulation/dehydration procedures. Shoot tips were excised under filter sterilized antioxidants solution (0.2M phosphate buffer, pH 5.7 supplemented with 5g/1 ascorbic acid and 15g/1 sodium borate). They were drawn up into a sterile 10 $\textrm{cm}^3$disposable pipette and were dropped into the culture medium with 2.5w/v Na-alginate, then into 100mM CaCl$_2$.2$H_2O$. Encapsulated shoot tips were transferred into 10㎤ of liquid culture medium with a range of sucrose concentrations (0.25-1.0M) and were incubated in dark for 24 hours in 18C at 40rpm. Beads were then dehydrated in silica gel for different time intervals (1-24 hours). Then they were freeze dried either rapidly (plunge directly into liquid N2 or in two stages (samples were kept at 20C for 10 minutes, then reduced to 35C at 1C per minute. Then, plunge into liquid $N_2$). The influence of sucrose and silica gel pre-treatment on pre- and post-freeze shoot growth was examined.(중략)