• 한국환경농학회지
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• 제32권4호
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• pp.252-259
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• 2013

간척지 논 토양에서 염 농도에 따른 메탄 배출특성을 조사하기 위하여 포트 실험을 실시한 결과, 염 농도의 증가는 메탄 배출량 감소와 벼 생육 및 수량성 악화에 영향을 주는 것으로 조사되었다. 벼 재배기간 중 높은 EC와 pH로 인한 메탄생성균의 활성 감소와 벼 생육 악화에 따른 메탄 배출량 감소가 주요 원인으로 평가되었다. 토양의 EC와 pH는 총 메탄배출량과 고도의 부의 상관관계를 나타내었으며, 벼 생육(초장 및 분얼)과는 정의 상관관계를 나타내었다. 하지만 주로 높은 EC에 의한 메탄 저감효과는 벼의 생육 초기에 대부분 나타났으며, 생육 후기로 갈수록 염의 희석효과에 의하여 저감효과가 크게 감소되는 것으로 확인하였다. 본 연구의 결과를 통하여 간척지 논 토양의 염 농도가 메탄 배출량에 감소에 큰 영향을 줄 수 있는 것으로 평가되며, 간척지 논 토양에서 메탄 배출량 평가 또는 예측에 좋은 자료로 활용될 수 있을 것으로 판단된다.

• Weed & Turfgrass Science
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• 제4권2호
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• pp.129-134
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• 2015

굼벵이는 각종 작물과 나무에 피해를 줄 뿐만 아니라 특히 골프장 잔디에 피해를 주는 주요해충의 하나이다. 본 연구는 18종의 식물정유(anise oil, camphor, castor, cinnamon, clove oil, citronella, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, rosemary, tea-tree, thyme, turpentine)를 이용하여 굼벵이의 환경친화적 방제 가능성을 알아보고자 실내와 온실, 야외에서 실험을 수행하였다. Cap vial실험에서 홈줄풍뎅이 3령충에 효과가 높은 정유는 anise (88.9%)와 linseed oil (100%) 및 tea-tree oil (88.9%)이었다. 온실 pot에서 왜콩풍뎅이 3령충에 대한 anise와 linseed oil의 효과는 낮게 나타났다. 야외에서 anise와 linseed 및 tea-tree oil의 효과도 대상 굼벵이의 종류에 따라 차이를 보였다. 주둥무늬차색풍뎅이 번데기에 대해서는 tea-tree oil 처리에서만 32.6%의 보정사충율을 보였으나 등얼룩풍뎅이 3령충에 대해서는 anise와 linseed, tea-tree oil이 각각 54.8%, 51.6%, 56.5%의 보정사충율을 보였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.25-25
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• 2022

Drought becomes frequent and severe because of continuous global warming, leading to a significant loss of crop yield. In soybean (Glycine max [L.]), most of quantitative trait loci (QTLs) analyses for drought tolerance have conducted by investigating yield changes under water-restricted conditions at the reproductive stages. More recently, the necessity of QTL studies to use physiological indices responding to drought at the early growth stages besides the reproductive ones has arisen due to the unpredictable and prevalent occurrence of drought throughout the soybean growing season. In this study, we thus identified QTLs conferring wilting scores and moisture contents of soybean subjected to drought stress in the early vegetative stage using an recombinant inbred line (RIL) population derived from a cross between Taekwang (drought-sensitive) and SS2-2 (drought-tolerant). For the two traits, the same major QTL was located on chromosome 10, accounting for up to 11.5% of phenotypic variance explained with LOD score of 12.5. This QTL overlaps with a reported QTL for the limited transpiration trait in soybean and harbors an ortholog of the Arabidopsis ABA and drought-induced RING-D UF1117 gene. Meanwhile, one of important features of plant drought tolerance is their ability to limit transpiration rates under high vapor pressure deficiency in response to mitigate water loss. However, monitoring their transpiration rates is time-consuming and laborious. Therefore, only a few population-level studies regarding transpiration rates under the drought condition have been reported so far. Via employing an Arduino-based platform, for the reasons addressed, we are measuring and recording total pot weights of soybean plants every hour from the 1^st day after water restriction to the days when the half of the RILs exhibited permanent tissue damage in at least one trifoliate. Gradual decrease in moisture of soil in pots as time passes refers increase in the severity of drought stress. By tracking changes in the total pot weights of soybean plants, we will infer transpiration rates of the mapping parents and their RILs according to different levels of VPD and drought stress. The profile of transpiration rates from different levels of severity in the stresses facilitates a better understanding of relationship between transpiration-related features, such as limited maximum transpiration rates, to water saving performances, as well as those to other drought-responsive phenotypes. Our findings will provide primary insights on drought tolerance mechanisms in soybean and useful resources for improvement of soybean varieties tolerant to drought stress.

• 한국약용작물학회지
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• 제5권3호
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• pp.186-190
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• 1997

This study was carried out to investigate the effect of polyamines, salt strength. sucrose and gelling agents on the regeneration of plantlets by meristem culture of Aloe arborescens Mill. and Aloe vera L.. Shoot multiplication was more effective when 10mg/ l spermine in Aloe arborescens and 1mg/ l spermidine in Aloe vera added into MS medium than when other polyamines were treated into media. A quarter strength of MS medium was effective for rooting of shoots regenerated. Higher concentration of sucrose (45g/ l) was more effective for shoot regeneration. Addition of 4g/ l gelrite into the medium was effective for induction of multiple shoots from Aloe than that of agar or other concentrations of gelrite. When plantlets regenerated from meristem culture were transferred to pot. survival rate of plantlets was 80% on perlite and was 95% on vermiculite. respectively.

• 한국초지조사료학회지
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• 제23권1호
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• pp.23-30
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• 2003

신개간 산지토양을 공시배지로 한 간이 pot 시험으로 초지조성을 위한 소석회와 규산질 비료 두 토양 개량제가 orchardgass/ladino clover 혼파목초의 초기생육상, 식생/수량구성비율 및 수양에 미치는 영향을 초지조성 초기단계에서 비교.검토하였다. 처리수준은 1) 대조구($L_{0}$ ; 석회 및 규산 무비구), 2) 소석회 소비구($L_1$; 3.75), 3) 소석회 보비구($L_2$; 7.50), 4) 소석회 다비구($L_3$; 11.25 ｇ/pot), 5) 규산질 비료 소비구($S_1$; 3.75), 6) 규산질 비료 보비구($S_2$; 7.50), 7) 규산질 비료 다비구($S_3$; 11.25 ｇ/pot)로 하였다. 1. 신개간지 공시토양은 불량한 이화학성을 보였다. 3요소 기준시비만 된 대조구($L_{0}$)의 초 기생육상은 불량하였으며, 특히 ladino clover의 정착이 매우 불량하였다. 반면에 소석회나 규산질 비료의 시용시에는 혼파목화의 정착 및 초기생육상이 크게 양호하여졌다. 달관조사기 준(1~9등급; 1= 매우 양호, 9= 매우 불량)에 따라 초기생육상을 처리별 비교하여 보면, $L_2$= $S_3$; 1.75 > $L_3$ = $S_2$; 2.25 > $S_1$; 2.50 > $L_1$; 3.75 >$ L_{0}$ ; 6.00 순으로 양호한 초기생육상을 보였다. 2. 대조구($L_{0}$ )에서는 ladino clover의 식생비율이 상대적으로 매우 불량한 평균 13.5% 수준으로 orchardgrass(86.5%)에 비해서 정착이 매우 불량하였다. 소석회나 규산질 비료의 시용으로 ladino clover의 식생비율이 크게 양호하여졌고, 이 때 규산질 비료가 소석회보다 더큰 효과를 보였다. 3. 소석회나 규산질 비료의 시용은 구성초종 중 orchardgrass의 수량향상에는 경미한 효과를 보였으나 ladino clover의 수량 향상에는 큰 효과를 보였다. 또한 ladino clover의 수량 향상에는 소석회보다 규산질 비료가 더 큰 효과를 보였고, 그 정도는 $L_1$의 수양에 비해서$ S_1$이 76.8% 더 증수하였고, $L_2$에 비해서는 $S_2$가 32.0% 더 증수하였다. orchardgrass 수량 향상에도 소석회보다 규산질 비료가 약간 양호한 경향을 보였다. 혼파목초의 총 수양은 처리별ladino clover의 수양성 변화 특성에 크게 영향을 받았다.

• 농업과학연구
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• 제49권2호
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• pp.357-366
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• 2022

Biochar can be obtained by using various types of biomass under an oxygen-limited condition. Biochar can be utilized for various applications such as soil improvement, waste management, growth promotion, and adsorption. Wood vinegar is produced by the process of pyrolysis wood biomass and is used as a growth promoter, for soil improvement, and as a feed additive. When wood vinegar is treated on soil, it acts to control soil pH, improve nutrient availability, and alleviate N₂O and NH₃ volatilization. The objective of this study was to evaluate the effect of biochar and wood vinegar on the growth of perilla and soil quality. The experiment was conducted by using a Wagner pot (1·5,000 a^-1) in a glass greenhouse. The biochar was produced by pyrolysis at 450℃ for 30 minutes using rice husk and rice straw. Wood vinegar was diluted to 1 : 500 (v·v^-1) and used in this experiement. In the results of a cultivation experiment, co-application of biochar and wood vinegar enhanced the growth of perilla. In particular, rice husk biochar affected the leaves of the perilla, and rice straw biochar influenced the stems of the perilla. In addition, soil quality after treatment with biochar and wood vinegar applied together was highest compared to other units. Therefore, it is anticipated that co-application of biochar and wood vinegar will be more productive and improve soil quality compared to individual utilization of biochar and wood vinegar.

• The Plant Pathology Journal
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• 제18권1호
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• pp.36-42
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• 2002

Resistance of perilla varieties to Botrytis cinerea LVF12 was evaluated, while antagonistic bacteria were selected and tested for their efficacy towards biological control of gray mold rot caused by B. cinerea. Among 11 perilla varieties tested for disease resistance, Milyang variety showed some degree of resistance, while the rest of varieties showed no resistance. Among 250 bacterial isolates collected from perilla loaves and rhizosphere of perilla plants, six isolates showed high levels of inhibitory effect on mycelial growth and conidial germination of B. cinerea in in vitro test. Using the pot test in growth chambers these isolates showed high levels of disease suppression, with Nl isolate showing 95.3% of control value and N4 isolate showing 90.8% of control value. Further test was performed to evaluate the two isolates ability for disease prevention and/or disease therapy, and results showed almost 100% of control vague. Isolates Nl and N4 were identified as Bacillus licheniformis and 5. megatepium, respectively, according to Bergey's manual, API 20E and 50CHB test kit, and Transmission electron microscope.

• Journal of Ginseng Research
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• 제45권2호
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• pp.218-227
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• 2021

Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119^T had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 10⁸ CFU/mL DCY119^T for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119^T can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119^T can potentially be used for bioremediation of HM contamination.

• The Plant Pathology Journal
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• 제39권6호
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• pp.575-583
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• 2023

Fusarium root rot is an increasingly severe problem in soybean cultivation. Although several Fusarium species have been reported to infect soybean roots in Heilongjiang province, their frequency and aggressiveness have not been systematically quantified in the region. This study aimed to investigate the diversity and distribution of Fusarium species that cause soybean root rot in Heilongjiang province over two years. A total of 485 isolates belonging to nine Fusarium species were identified, with F. oxysporum and F. solani being the most prevalent. Pot experiments were conducted to examine the relative aggressiveness of different Fusarium species on soybean roots, revealing that F. oxysporum and F. solani were the most aggressive pathogens, causing the most severe root rot symptoms. The study also assessed the susceptibility of different soybean cultivars to Fusarium root rot caused by F. oxysporum and F. solani. The results indicated that the soybean cultivar DN51 exhibited the most resistance to both pathogens, indicating that it may possess genetic traits that make it less susceptible to Fusarium root rot. These findings provide valuable insights into the diversity and distribution of Fusarium species that cause soybean root rot and could facilitate the development of effective management strategies for this disease.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1040-1050
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• 2024

To isolate and analyze bacteria with Verticillium wilt-resistant properties from the fermentation residue of kitchen wastes, as well as explore their potential for new applications of the residue. A total of six bacterial strains exhibiting Verticillium wilt-resistant capabilities were isolated from the biogas residue of kitchen waste fermentation. Using a polyphasic approach, strain ZL6, which displayed the highest antagonistic activity against cotton Verticillium wilt, was identified as belonging to the Pseudomonas aeruginosa. Bioassay results demonstrated that this strain possessed robust antagonistic abilities, effectively inhibiting V. dahliae spore germination and mycelial growth. Furthermore, P. aeruginosa ZL6 exhibited high temperature resistance (42℃), nitrogen fixation, and phosphorus removal activities. Pot experiments revealed that P. aeruginosa ZL6 fermentation broth treatment achieved a 47.72% biological control effect compared to the control group. Through activity tracking and protein mass spectrometry identification, a neutral metalloproteinase (Nml) was hypothesized as the main virulence factor. The mutant strain ZL6ߡNml exhibited a significant reduction in its ability to inhibit cotton Verticillium wilt compared to the strain P. aeruginosa ZL6. While the inhibitory activities could be partially restored by a complementation of nml gene in the mutant strain ZL6CMߡNml. This research provides a theoretical foundation for the future development and application of biogas residue as biocontrol agents against Verticillium wilt and as biological preservatives for agricultural products. Additionally, this study presents a novel approach for mitigating the substantial amount of biogas residue generated from kitchen waste fermentation.