• 제목/요약/키워드: post-curing

검색결과 138건 처리시간 0.022초

큐어링 후 저장에 따른 고구마 저장뿌리 단백질체의 비교분석 (Comparative proteome profiling in the storage root of sweet potato during curing-mediated wound healing)

  • 신호용;지창윤;김호수;정정성;최성환;곽상수;김윤희;이증주
    • Journal of Plant Biotechnology
    • /
    • 제50권
    • /
    • pp.1-10
    • /
    • 2023
  • 고구마(Ipomoea batatas L. Lam)는 영양소, 가공 식품, 동물 사료 및 색소 재료의 유용한 공급원으로 이용 가능한 경제적으로 중요한 대표적인 뿌리 작물이다. 일반적으로 고구마의 저장 뿌리는 수확 후 저장 기간 동안 다양한 미생물과 질병에 의한 부패에 노출되기 쉽다. 수확 후 큐어링은 저장기간 동안 상처를 치유하고 미생물에 의한 부패를 방지하기 위한 가장 적합한 수단으로 알려져 있다. 본 연구에서는 큐어링과 연관된 분자적 기작에 관여하는 단백질들을 확인하기 위해, 큐어링 처리 후 저장기간 동안 단백질체의 변화를 분석하였다. 33℃ (큐어링) 및 15℃ (대조군)에서 3일 동안 처리하고 8주의 저장 기간이 지난 후 2D 전기영동 분석을 통해 단백질 spot의 변화를 확인한 결과, 31개 단백질 spot의 발현량이 차이 나는 것을 확인하였으며, 이들 중 15개의 단백질 spot을 동정하여 그 특성을 분석하였다. 동정된 단백질 중 alphaamylase (spot 1)는 큐어링 처리구에서만 발현량이 증가하였으며, probable aldo-keto reductase 2-like (spot 3) 및 hypothetical protein CHGG_01724 (spot 4)는 큐어링 및 대조구에서 동시에 발현량이 증가하였으나, sporamin A (spot 10)는 큐어링 및 대조구에서 발현량이 감소하였다. 한편, 대조구에서 enolase (spot 14)는 발현량이 증가하였으나, chain A of actinidin-E-64 complex+ (spot 19), ascorbate peroxidase (spot 22) 및 여러 sporamin 단백질들(spot 20, 21, 23, 24, 27, 29, 30 및 31)은 발현량이 감소하였다. 본 연구의 결과는 고구마 저장 뿌리에서 큐어링 처리와 관련된 단백질의 동정 및 수확 후 저장 기간동안 병 저항성과 관련된 기작에 대한 이해를 높이며, 향후 저온 저장 능력이 향상된 신품종 개발을 위한 후보 유전자의 도출에도 기여할 수 있을 것이다.

Effect of dentin pretreatment and curing mode on the microtensile bond strength of self-adhesive resin cements

  • Youm, Seung-Hyun;Jung, Kyoung-Hwa;Son, Sung-Ae;Kwon, Yong-Hoon;Park, Jeong-Kil
    • The Journal of Advanced Prosthodontics
    • /
    • 제7권4호
    • /
    • pp.317-322
    • /
    • 2015
  • PURPOSE. The aim was to evaluate the effect of curing mode and different dentin surface pretreatment on microtensile bond strength (${\mu}TBS$) of self-adhesive resin cements. MATERIALS AND METHODS. Thirty-six extracted human permanent molars were sectioned horizontally exposing flat dentin surface. The teeth were divided into 12 groups (3 teeth/group) according to the dentin surface pretreatment methods (control, 18% EDTA, 10% Polyacrylic acid) and curing mode (self-curing vs. light-curing) of cement. After pretreatment, composite resin blocks were cemented with the following: (a) G-CEM LinkAce; (b) RelyX U200, followed by either self-curing or light-curing. After storage, the teeth were sectioned and ${\mu}TBS$ test was performed using a microtensile testing machine. The data was statistically analyzed using one-way ANOVA, Student T-test and Scheffe's post-hoc test at P<.05 level. RESULTS. For G-CEM LinkAce cement groups, polyacrylic acid pretreatment showed the highest ${\mu}TBS$ in the self-cured group. In the light-cured group, no significant improvements were observed according to the dentin surface pretreatment. There were no significant differences between curing modes. Both dentin surface pretreatment methods helped to increase the ${\mu}TBS$ of RelyX U200 resin cement significantly and degree of pretreatment effect was similar. No significant differences were found regarding curing modes except control groups. In the comparisons of two self-adhesive resin cements, all groups within the same pretreatment and curing mode were significantly different excluding self-cured control groups. CONCLUSION. Selecting RelyX U200 used in this study and application of dentin surface pretreatment with EDTA and polyacrylic acid might be recommended to enhance the bond strength of cement to dentin.

시트양생 및 파이프 쿨링에 의한 매스콘크리트 구조물의 온도제어 (Temperature Control of Mass-Concrete Structure with Pipe Cooling or Sheet Curing.)

  • 차홍윤;김은경;김래현;신치범
    • 한국콘크리트학회:학술대회논문집
    • /
    • 한국콘크리트학회 1995년도 봄 학술발표회 논문집
    • /
    • pp.263-267
    • /
    • 1995
  • The usual methods for the temperature control of mass-concrete structures include the use of low-heat cement, pre-cooling, post-cooling, or sheet curing. In order to control the heat of hydration during the construction of mass-concrete structures, the combination of the above methods is commonly employed. For the construction of mass-concrete structures such as massive pier or anchor, it is necessary to control the curing temperature with pipe cooling. In this study, the method of analysis on the effect of pipe of was proposed to prevent the thermal cracking due to heat of hydration In addition the effect of covering the concrete surface with blanket insulation was investigated. The results of the present study may be useful for the prediction of curing temperature of mass-concrete structures and the reasonable construction management.

  • PDF

Investigation of the effect of internal curing as a novel method for improvement of post-fire properties of high-performance concrete

  • Moein Mousavi;Habib Akbarzadeh Bengar
    • Computers and Concrete
    • /
    • 제33권3호
    • /
    • pp.309-324
    • /
    • 2024
  • Internal curing, a widely used method for mitigating early-age shrinkage in concrete, also offers notable advantages for concrete durability. This paper explores the potential of internal curing by partial replacement of sand with fine lightweight aggregate for enhancing the behavior of high-performance concrete at elevated temperatures. Such a technique may prove economical and safe for the construction of skyscrapers, where explosive spalling of high-performance concrete in fire is a potential hazard. To reach this aim, the physico-mechanical features of internally cured high-strength concrete specimens, including mass loss, compressive strength, strain at peak stress, modulus of elasticity, stress-strain curve, toughness, and flexural strength, were investigated under different temperature exposures; and to predict some of these mechanical properties, a number of equations were proposed. Based on the experimental results, an advanced stress-strain model was proposed for internally cured high-performance concrete at different temperature levels, the results of which agreed well with the test data. It was observed that the replacement of 10% of sand with pre-wetted fine lightweight expanded clay aggregate (LECA) not only did not reduce the compressive strength at ambient temperature, but also prevented explosive spalling and could retain 20% of its ambient compressive strength after heating up to 800℃. It was then concluded that internal curing is an excellent method to enhance the performance of high-strength concrete at elevated temperatures.

후경화를 고려한 HTPB 고체 추진제의 실험적 노화평가 기법 연구 (Study on the Experimental Aging Estimation Technique for HTPB based Solid Propellant Considering Post Curing Effect)

  • 정규동;박재범;김신회
    • 한국추진공학회지
    • /
    • 제23권3호
    • /
    • pp.51-57
    • /
    • 2019
  • HTPB 고체 추진제 기계적 물성의 후경화 효과에 대하여 시편 시험을 통하여 평가하였다. 후경화 반응 완료 후 시편 가속노화 시험을 실시하여 Arrhenius 식의 계수를 획득하였다. 확인을 위하여 원통형 모사 충전체를 설계, 제작하여 가속노화 시험을 수행하였으며, 시험 후 추진제 표면 부위, 중앙 부위, 접착 부위에 대하여 시편을 채취하여 노화 물성을 평가하였다. 측정된 결과에 대하여, 획득된 가속노화 식으로 예측하여 비교하였다. 그 결과 JANNAF 시편 시험을 통한 가속노화 식은 추진제 재료의 노화를 잘 예측하였으나 접착 부위에서는 실제 측정 결과와 차이를 나타내었다. 따라서 실기형 추진기관에서 노화 시료를 채취하는 경우 시험 목적에 부합하도록 시편 채취 부위를 선정해야 한다.

동시경화 하이브리드 금속/복합재료 구조물의 제조 잔류 열응력 제거를 위한 경화사이클에 관한 연구 (Investigation of cure cycle for co-cured metal/composite hybrid structures without fabricating thermal residual stress)

  • 김학성;박상욱;이대길
    • 한국복합재료학회:학술대회논문집
    • /
    • 한국복합재료학회 2004년도 추계학술발표대회 논문집
    • /
    • pp.83-87
    • /
    • 2004
  • In this work, the cure cycle of co-cured metal/composite structure was investigated to decrease fabricating thermal residual stresses between the metal and the composite material. DSC (Differential scanning calorimetry) experiment and static lap shear test of co-cured aluminum/composite double lap joint as well as the curvature experiment of co-cured steel/composite strip were performed to investigate the effect of curing cycle on the thermal residual stress of co-cured hybrid structures. From the experiments, it was found that post curing method after abrupt cooling of co-cured aluminum/composite hybrid structure at certain point of degree of cure during curing process could eliminate fabricating the thermal residual stresses.

  • PDF

다양한 중합 방법으로 제작한 간접 수복물용 복합레진의 굴곡 강도 (Flexural strength of composite resin fabricated by various polymerization method)

  • 김동연;박진영;강후원;김지환;김웅철
    • 대한치과기공학회지
    • /
    • 제40권2호
    • /
    • pp.57-62
    • /
    • 2018
  • Purpose: The aim of this study is to evaluate composite resins of indirect restorations for testing of flexural strength according to various polymerization methods. Methods: Specimen was produced a total of 40 to 10 per each group with a length 25 mm, width 2 mm, thickness 2 mm using a Teflon zig. The polymerization groups were classified into four groups. The first group proceeded with light curing only(LC group). The second group proceeded with light and heat curing(LHC group). The third group proceeded with air press and light curing(ALC group). The fourth group proceeded with air press, light and heat curing(ALHC group). Each prepared group was evaluated by flexural strength test. Statistical analysis was performed by one-way ANOVA. Post-test was performed with Tukey test. Results: The lowest in the ALC group was 119.18 MPa and the highest in the ALHC group was 168.15 MPa. There were statistically significant differences. Conclusion : The composite resin of the indirect restoration is recommended to heat curing along with the air press.

Flexural properties of a light-cure and a self-cure denture base materials compared to conventional alternatives

  • Mumcu, Emre;Cilingir, Altug;Gencel, Burc;Sulun, Tonguc
    • The Journal of Advanced Prosthodontics
    • /
    • 제3권3호
    • /
    • pp.136-139
    • /
    • 2011
  • PURPOSE. A new light curing urethane dimethacrylate and a cold curing resin with simpler and faster laboratory procedures may have even improved flexural properties. This study investigated the 3-point flexural strengths and flexural moduli of two alternate base materials. MATERIALS AND METHODS. A cold curing resin (Weropress) and a light curing urethane dimethacrylate base material (Eclipse). Along with Eclipse and Weropress, a high impact resin (Lucitone199) and three conventional base materials (QC 20, Meliodent and Paladent 20) were tested. A 3-point bending test was used to determine the flexural strengths and flexural moduli. The mean displacement, maximum load, flexural modulus and flexural strength values and standard deviations for each group were analyzed by means of one-way analysis of variance (ANOVA) (with mean difference significant at the 0.05 level). Post hoc analyses (Scheffe test) were carried out to determine the differences between the groups at a confidence level of 95%. RESULTS. Flexural strength, displacement and force maximum load values of Eclipse were significantly different from other base materials. Displacement values of QC 20 were significantly different from Lucitone 199 and Weropress. CONCLUSION. The flexural properties and simpler processing technique of Eclipse system presents an advantageous alternative to conventional base resins and Weropress offers another simple laboratory technique.

Shear performance of an innovative UHPFRC deck of composite bridge with coarse aggregate

  • Qi, Jianan;Wanga, Jingquan;Feng, Yu
    • Advances in concrete construction
    • /
    • 제7권4호
    • /
    • pp.219-229
    • /
    • 2019
  • This paper presents an experimental study on the structural performance of an innovative ultra-high performance fiber reinforced concrete (UHPFRC) deck with coarse aggregate of composite bridge under shear force. Test parameters included curing method and shear span-to-height ratio. Test results indicated that more short fine cracks developed beside the existing cracks due to the randomly dispersed fibers, resulting in re-distributing and homogenizing of the concrete stress beside cracks and allowing for the occurrence of more cracks with small spacing compared to normal strength concrete beams. Curing methods, incorporating steam curing and natural curing, did not have obvious effect on the nominal bending cracking strength and the ultimate strength of the test specimens. Shear reinforcement need not be provided for UHPFRC decks with a fiber volume fraction of 2%. UHPFRC decks showed superior load resistance ability after the appearance of cracks and excellent post-cracking deformability. Lastly, the current shear provisions were evaluated by the test results.

글리세린이 복합레진의 중합 후 표면경도에 미치는 영향 (Effect of glycerin on the surface hardness of composites after curing)

  • 박현희;이인복
    • Restorative Dentistry and Endodontics
    • /
    • 제36권6호
    • /
    • pp.483-489
    • /
    • 2011
  • 연구목적: 본 연구의 목적은 글리세린의 도포가 복합레진의 중합 후 표면경도에 미치는 효과를 알아보고자 하였다. 연구 재료 및 방법: 디스크 형태의 황동 몰드에 복합레진(Z-250, 3M ESPE)을 충전 후 다음과 같이 광중합 하였다. 1군(대조군)은 복합레진 표면을 공기 중에 노출한 후 40초 동안 광조사, 2군은 표면을 Mylar strip으로 덮은 후 40초 광조사, 3군은 표면을 glycerin으로 도포 후 40초 광조사, 4군은 공기 중에 노출시켜 20초 광조사한 다음 시편 표면에 glycerin으로 도포한 후 추가로 20초 동안 광조사 하였다. 각 그룹당 20개의 시편을 준비하였고, 연마 후 또는 연마를 하지 않고 시편의 표면경도를 측정하였다. 5일 후 각 시편의 표면경도를 재 측정하였다. 데이터는 three-way ANOVA와 Tukey's post hoc test를 이용하여 분석하였다. 결과: 중합 직 후, 연마하지 않은 시편의 표면경도는 2군 > 3군 > 4군 > 1군의 순이었다. 연마한 경우 각 군 사이의 유의한 차이는 없었다. 같은 군 내에서는 5일 후 표면경도가 중합 직 후 보다 증가하였고 연마한 경우가 연마하지 않은 경우보다 더 높은 표면경도를 나타냈다. 결론: 중합 후 복합레진의 표면경도를 증가시키기 위한 가장 좋은 방법은 연마였고, 중합 전 Mylar strip의 사용이나 glycerin의 도포 역시 중합된 복합레진의 표면경도를 증가시켰다.