Aggregation of normally soluble proteins can cause disease-related problems. Tryptophan synthase α-subunit (αTS) in E. coli adopts one of most popular structural scaffolds, the TIM barrel fold. Previous mutagenesis of the αTS gene resulted in many aggregation-prone mutant proteins. Here, Y173F (Tyr at residue 173 to Phe) substitution, which imparts increased stability, was tested for its ability to suppress aggregation of aggregation-prone mutant proteins (Y4C, S33L, P28L, P28S, G44S, D46N, P96L, and P96S). Aggregation was suppressed in all eight severe aggregate-forming mutants (all differing in their mutation positions), by the Y173F replacement. P28L αTS, which was available in pure form, was further analyzed and showed reduced secondary structure content, lower stability, and a looser structure with more exposed hydrophobic surface compared to the wild type protein. A double mutant P28L/Y173F protein showed almost no indication of these changes compared to the wild type protein. We hypothesized that Tyr at position 173 in αTS is positioned at the hydrophobic core and may serve to suppress the aggregation of this protein caused by other residues. Important residue (s) could be working widely in the prevention/suppression of protein aggregation.
According to reinforce environmental regulations, coal power plants have used selective catalytic reduction using ammonia as a reducing agent to reduce the amount of nitrogen oxide generation. The purpose of the present study was to derive a mixing device for effectively mixing dilute air and ammonia in the ammonia mixing pipe by performing computational fluid dynamic analysis. The mixing effect was compared by analysing the %RMS of ammonia concentration at the down stream cross section in the mixing pipe and the 16 outlets based on the case 1-1 shape, which is an existing mixing pipe without a mixing device. The mixing device was performed by changing the positions of a square plate on the downstream side of the ammonia supply pipe and an arc-shaped plate on the wall of the mixing pipe. In the case of the existing geometry(Case 1-1), the %RMS of ammonia concentration at the 16 outlets was 29.50%. The shape of the mixing device for Case 3-2 had a square plate on the downstream side of the ammonia supply pipe and an arc plate was installed adjacent to it. The %RMS of ammonia concentration for Case 3-2 was 2.08% at 16 outlets and it could be seen that the shape of Case 3-2 was the most effective mixing shape for ammonia mixing.
Materials and techniques used for two portraits (Jo-nyeon Lee and S ung-in Lee) of the Lee family from Seongju enshrined in Seongsan temple were scientifically analyzed, and based on the data, an optimal replica restoration method was designed. According to the expression technique investigation, both portraits were expressed mainly in line drawing, but there were differences in shoes, pupils, the color expression of flesh, overpainting, and traces of reinforcement. Pigment analysis revealed that a mixture of cinnabar and minium, organic pigment, azurite, malachite, lead white, and yellow pigment were used in common. In the case of Sung-in Lee's portrait, seokganju and atacamite were also used. In addition, comparison with the contemporaneous portraits of gentry showed that the portrait style at the time was found in the two portraits, but the singularity was modified differently there. Based on the scientific analysis, it was decided to replicate the old color restoration for Jo-nyeon Lee's portrait while for Sung-in Lee's portrait, it was decided to replicate the phenomenon. Detailed coloring techniques were presented by supplementing the expression techniques that are difficult to confirm visually using scientific data. In addition, by measuring the chromaticity of representative positions in the portrait for each color and presenting the color reference value calculated as the average value, the current color of the artifact can be replicated and restored based on the objective data as much as possible.
International Journal of Computer Science & Network Security
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v.22
no.12
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pp.63-70
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2022
The essence of the definition of "tolerance" is analyzed. Motivational, knowledge and behavioral criteria for tolerance of future teachers are highlighted. Indicators of the motivational criterion are the formation of value orientations, motivational orientation, and the development of empathy. Originality and productivity of thoughts and judgments, tact of dialogue, pedagogical ethics and tact are confirmed as indicators of the knowledge criterion. The behavioral criterion includes social activity as a life position, emotional and volitional endurance, and self-control of one's own position. The formation of tolerance is influenced by a number of factors: the social environment, the information society, existing stereotypes and ideas in society, the system of education and relationships between people, and the system of values. The main factors that contribute to the education of tolerance in future teachers are highlighted. Analyzing the structure of tolerance, it is necessary to distinguish the following functions of tolerance: - motivational (determines the composition and strength of motivation for social activity and behavior, promotes the development of life experience, because it allows the individual to accept other points of view and vision of the solution; - informational (understanding the situation, the personality of another person); - regulatory (tolerance has a close connection with the strong - willed qualities of a person: endurance, selfcontrol, self-regulation, which were formed in the process of Education); - adaptive (allows the individual to develop in the process of joint activity a positive, emotional, stable attitude to the activity itself, which the individual carries out, to the object and subject of joint relations). The implementation of pedagogical functions in the information society: educational, organizational, predictive, informational, communicative, controlling, etc. provides grounds to consider pedagogical tolerance as an integrative personal quality of a representative of any profession in the field of "person-person". The positions that should become conditions for the formation of tolerance of the future teacher in the information society are listed.
Journal of the Korean Society of Earth Science Education
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v.16
no.2
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pp.261-275
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2023
The purpose of this study was to analyze the conceptual understanding of carbon neutrality among secondary school science pre-service teachers, as well as to identify gaze patterns in visual materials. For this study, gaze tracking data of 20 pre-service secondary school science teachers were analyzed. Through this, the levels of conceptual understanding of carbon neutrality were categorized for the participants, and differences in gaze patterns were analyzed based on the degree of conceptual understanding of carbon neutrality. The research findings are as follows. First, as a result of performing modeling activities to predict carbon emissions and removals until 2100 using the concept of '2050 carbon neutrality,' 50% of the participants held a conception that carbon emissions would continue to increase. Additionally, 25% of the participants did not properly understand the causal relationship between net carbon dioxide emissions and cumulative concentrations. Second, the gaze movements of the participants regarding visual materials related to carbon neutrality were significantly influenced by the information presented in the text area, and in the case of graphs, the focus was mainly on the data area. Moreover, when visual data with the same function and category were arranged, participants showed the most interest in materials explaining concepts or visual data placed on the left side. This implies a preference for specific positions or orders. Participants with lower levels of conceptual understanding and inadequate grasp of causal relationships among elements exhibited notably reduced concentration and overall gaze flow. These findings suggest that conceptual understanding of carbon neutrality including climate change and natural disaster significantly influences interest in and engagement with visual materials.
This study examined the reasoning of gifted elementary science students in a socioscientific issues (SSI) classroom discussion on COVID-19-related trash disposal challenges. This study aimed to understand the characteristics of evidence use and decision-making difficulties in each type of SSI-related reasoning. To this end, the transcripts of 17 gifted students of elementary science discussing SSIs in a classroom were analyzed within the framework of informal reasoning. The analysis framework was categorized into three types according to the primary influence involved in reasoning: rational, emotional, and intuitive. The analysis showed that students exhibited four categories of evidence use in SSI reasoning. First, in the rational reasoning category, students deemed and recorded scientific knowledge, numbers, and statistics as objective evidence. However, students who experienced difficulty in investigating such scientific data were less likely to have factored them in subsequent decisions. Second, in the emotional reasoning category, students' solutions varied considerably depending on the perspective they empathized with and reasoned from. Differences in their views led to conflicting perspectives on SSIs and consequent disagreement. Third, in the intuitive reasoning category, students disagreed with the opinions of their peers but did not explain their positions precisely. Intuitive reasoning also created challenges as students avoided problem-solving in the discussion and did not critically examine their opinions. Fourth, a mixed category of reasoning emerged: intuition combined with rationality or emotion. When combined with emotion, intuitive reasoning was characterized by deep empathy arising from personal experience, and when combined with rationality, the result was only an impulsive reaction. These findings indicate that research on student understanding and faculty knowledge of SSIs discussed in classrooms should consider the difficulties in informal reasoning and decision-making.
This article introduces Abe Mituie's activities related to Korean and Japanese Buddhism and two newly discovered materials. He worked as a brain of Japanese cultural rule over Joseon Korea while holding various positions such as the president of KyeongSung Il Bo, the vice president of Kokmin Newspaper and the director of Central Joseon Association. Abe was responsible for Enkak Temple, the head temple of Japanese Rinzai section, and was one of the layman followers of Syak Soen who worked for the spread of modern Japanese Buddhism to Europe and America. He was a respectful Buddhist layman so as to teach Zen Buddhism for young Buddhist monks in Japan. After he started to assume charge in the Kyeong Sung Il Bo, he was also very active in movements in relation to Joseon Buddhism to the extent that he was found to be deeply involved in Joseon Buddhism sections. On the other hand, he concluded Joseon culture to be 'devastated.' He asserted that it was necessary to develop spiritual culture and revive Buddhism in order to resolve the devastation in the Joseon. In addition, he thought that Joseon Buddhism was ruined due to the misgovernment of the Joseon Dynasty, but had its own as great tradition as Japanese Buddhism. Therefore, in his opinion, there was a need to do research on Joseon Buddhism and find some way out of the contemporary difficulties. In order to save the situation, he made efforts to protect and revive Joseon Buddhism while paying continuous visits to Joseon Buddhist temples, supporting the publication of Buddhist canons and proposing to have a regular meeting of 'The Invitation of 30 Head Temples.' From his visit to Youngju Temple and his consistent relationship with Kang Daeryeon, it can be assumed that he was involved in reorganizing power structure in Joseon Buddhism and establishing various institutions. He emphasized the strict adherence of individuals and communities to rules in his lecture for students at Jung Ang Hak Rim. It was a way to revive Joseon Buddhism by creating a new social image of Joseon Buddhism. He continued to work for the restoration of Joseon Buddhism even after he retired from Kyeong Sung Il Bo and returned to Japan. He introduced the originality of Joseon Buddhism history to Japan and sent Japanese monks to Korea in order to do research and contribute to exchange between Korean and Japanese Buddhism. All things taken together, it is evident that Abe Mitzihe regarded Joseon as backward or stagnant from a perspective of evolutionist or orientalist, and was a Japanese elite to believe that it was just for Japan to control Korea. However, he was different from other Japanese elites in that he did not considered Joseon Buddhism merely as the object of propagation. He thought that Joseon Buddhism possessed its own great tradition and culture, but was ruined because of the misadministration of the Joseon Royal House. Therefore, in his opinion, Joseon Buddhism should be recovered by means of some supports, and its revival would lead to the restoration of Joseon culture as a whole, which would be realized by Japanese rule over Korea and Japanese elites' generous assistance.
In this article I would like to propose the characteristics of "Village Council(Consejo del Pueblo)" as a sociopolitical device and institution, which maintains and encompasses all aspects of governance in a decentralized authority. A brief definition of Village Council is a "select group of people from the same village who are in charge of deciding the most important affairs of the village". The Village Council took charge of the most fundamental aspects of prehispanic Mayan society, most significantly, during the Late Postclassical era. The members of this council were respected people from the village, but not necessarily the people who held high positions within the hierarchical structure of Mayan society. It is likely that they were selected by the public in a direct way. This is the most essential bureaucratic institution in terms of the control of the political and socioeconomical affairs. The important decisions regarding legislation, the governance and jurisdiction of the village were entrusted to the council. Especially, the Village Council was responsible for monitoring and punishing the abuses of village governors. The majority of the members of the council were elders, consequently, the council was a political institution, more powerful than the governorships. The "Multepal" of Yucatán, could be an example of the Village Council during the late postclassic period.
The Journal of the Convergence on Culture Technology
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v.9
no.1
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pp.663-668
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2023
Recently it is very important to collect information such as enemy positions and facilities. To this end, unmanned aerial vehicles such as multicopters have been actively developed, and various mission equipment mounted on unmanned aerial vehicles have also been developed. The coordinate-oriented algorithm refers to an algorithm that calculates a gaze angle so that the mission equipment can fix the gaze at a desired coordinate or position. Flight data and GPS data were collected and simulated using Matlab for coordinate-oriented algorithms. In the simulation using only the coordinate data, the average Pan axis angle was about 0.42°, the Tilt axis was 0.003°~0.43°, and the relatively wide error was about 0.15° on average. As a result of converting this into the distance in the NE direction, the error distance in the N direction was about 2.23m on average, and the error distance in the E direction was about -1.22m on average. The simulation applying the actual flight data showed a result of about 19m@CEP. Therefore, we conducted a study on the self-error of coordinate-oriented algorithms in monitoring and information collection, which is the main task of EOTS, and confirmed that the quantitative target of 500m is satisfied with 30m@CEP, and showed that the desired coordinates can be directed.
Korean Journal of Construction Engineering and Management
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v.24
no.2
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pp.13-23
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2023
Unlike building construction projects, where the activity is repeatedly carried out in a limited area, civil engineering projects such as roads and railroads are carried out in a linear type in a horizontal working space over several tens of kilometers. Each activity is managed with a station number that has a unit of distance from the starting point to the end point. For this reason, since the work location information of the activity is a major management factor, the Gantt chart system that expresses only schedule information may have limitations. In this study, authors propose a method for constructing a linear schedule chart that can simultaneously express schedule information indicating the start and finish dates and location information indicating the start and end positions of each activity, and develop a system for generating a linear schedule chart. In the study, the coordinate axes of the linear schedule chart consisted of distance and date values on the X and Y axes, respectively, and each activity was expressed as a symbol that can infer the type of work to increase the visibility of the linear schedule chart compared to the simple bar chart method. The linear schedule chart generation system was reviewed for practical applicability by utilizing the actual schedule data of bridge structures in a railroad project.
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