• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.874-880
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• 2010

A series of 13- and 14-membered cyclic enkephalin analogs based on the moderately $\mu$ selective prototype compound Tyr-C[D-$A_2bu$-Gly-Phe-Leu] 8a were synthesized to investigate the structure-activity relationship. The modifications of sequence were mainly focused on two positions 3 and 5, critical for the selective recognition for $\mu$ and $\delta$ opioid receptors. The substitution of hydrophobic $Leu^5$ with hydrophilic $Asp^5$ derivatives led to Tyr-C[D-$A_2bu$-Gly-Phe-Asp(N-Me)] 7 and Tyr-C[D-Glu-Phe-gPhe-rAsp(O-Me)] 5, the peptides with a large affinity losses at both $\mu$ and $\delta$ receptors. The substitution of $Phe^3$ with $Gly^3$ led to Tyr-C[D-Glu-Gly-gPhe-rLeu] 3 and Tyr-C[D-Glu-Gly-gPhe-D-rLeu] 4, the peptides with large affinity losses at $\mu$ receptors, indicating the critical role of phenyl ring of $Phe^3$ for $\mu$ receptor affinities. One atom reduction of the ring size from 14-membered analogs Tyr-C[D-Glu-Phe-gPhe-(L and D)-rLeu] 6a, 6b to 13-membered analogs Tyr-C[D-Asp-Phe-gPhe-(L and D)-rLeu] 1, 2 reduced the affinity at both $\mu$ and $\delta$ receptors, but increased the potency in the nociceptive assay, indicating the ring constrain is attributed to high nociceptive potency of the analogs. For the influence of D- or L-chirality of $Leu^5$ on the receptor selectivity, regardless of chirality and ring size, all cyclic diastereomers displayed marked $\mu$ selectivity with low potencies at the $\delta$ receptor. The retro-inverso analogs display similar or more active at $\mu$ receptor, but less active at $\delta$ receptor than the parent analogs.

• Biomedical Science Letters
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• v.24 no.3
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• pp.221-229
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• 2018

Trichophyton rubrum is one of the well-known pathogenic fungi and causes dermatophytosis and cutaneous mycosis in human world widely. However, there are not an available sequence type (ST) classification methods and previous studies for T. rubrum until now. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to characterize the genetic diversity and the phylogenetic relation of T. rubrum clinical isolates, five different housekeeping genes, such as actin (ACT), calmodulin (CAL), RNA polymerase II (RPB2), superoxide dismutase 2 (SOD2), and ${\beta}$-tubulin (BT2) were analyzed using by multilocus sequence typing (MLST). Also, DNA sequence analysis was performed to examine the differences between the sequences of Trichophyton strains and the identified genetic variations sequence. As a result, most of the sequences were shown to have highly matched rates in their housekeeping genes. However, genetic variations were found on three different positions of ${\beta}$-tubulin gene and were shown to have changed from $C{\rightarrow}G$ (1766), $G{\rightarrow}T$ (1876), and $C{\rightarrow}A$ (1886). To confirm the association with T. rubrum inheritance, a phylogenetic tree analysis was performed. It was classified as four clusters, but there was little significant correlation. Even so, MLST analysis is believed to be helpful for determining the genetic variations of T. rubrum in cases where there is more large-scale data accumulation. In conclusion, the present study demonstrated the first MLST analysis of T. rubrum in Korea and explored the possibility that MLST could be a useful tool for studying the epidemiology and evolution of T. rubrum through further studies.

• BMB Reports
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• v.40 no.1
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• pp.95-99
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• 2007

Bovine coding region single nucleotide polymorphisms located proximal to quantitative trait loci were identified to facilitate bovine QTL fine mapping research. A total of 692,763 bovine SNPs was extracted from 39,432 UniGene clusters, and 53,446 candidate SNPs were found to be a depth >3. In order to validate the in silico SNPs experimentally, 186 animals representing 14 breeds and 100 mixed breeds were analyzed. Genotyping of 40 randomly selected candidate SNPs revealed that 43% of these SNPs ranged in frequency from 0.009 to 0.498. To identify non-synonymous SNPs and to correct for possible frameshift errors in the ESTs at the predicted SNP positions, we designed a program that determines coding regions by protein-sequence referencing, and identified 17,735 nsSNPs. The SNPs and bovine quantitative traits loci informations were integrated into a bovine SNP data: BcSNPdb (http://snugenome.snu.ac.kr/BtcSNP/). Currently there are 43 different kinds of quantitative traits available. Thus, these SNPs would serve as valuable resources for exploiting genomic variation that influence economically and agriculturally important traits in cows.

• Fashion & Textile Research Journal
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• v.12 no.4
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• pp.467-476
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• 2010

The purpose of this study was to develop a plausible methodology based on experimental data how to set up darts and split lines on 3D parametric body dressed with tight-fit garment. The results were as following: Through the process of making convex hull, the concave parts were straightened to make a convex hull, especially in the center part of bust, under breast part and scapular part. To figure out the optimum positions of darts and split lines, the inflection points of curve ratio were searched along the horizontal polylines of waist and bust. This procedures produced reliable results with low deviation. Using Rapidform, CATIA and Unigraphics, six patches of bodice patterns were drawn and aligned. Paired t-test results showed the outline and area between 3D surface and 2D were not significantly different, meaning this method could be adaptable when flattening 3D surfaces. The amount of waist dart measured on the pattern showed that the highest portion was allocated on 2nd dart(back), followed by 1st dart(back), 1st dart(front), 2nd dart(front)/side dart, and center back dart. A series of findings suggested that curve ration inflection point could be used as a guide to set up darts and split line on 3D parametric model with low deviation.

• Wind and Structures
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• v.13 no.6
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• pp.529-556
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• 2010

This paper investigates the correlation of wind characteristics monitored on a cable-stayed bridge. Total five anemoscopes are implemented into the bridge. Two out of 5 anemoscopes in inflow and two out of 5 anemoscopes in wake-flow along the longitudinal direction of the bridge are installed. Four anemoscopes are respectively distributed at two cross-sections. Another anemoscope is installed at the top of the tower. The correlation of mean wind speed and direction, power spectral density, the turbulent intensity and integral length of wind in flow at two cross-sections are investigated. In addition, considering the non-stationary characteristics of wind, the spatial correlation in time-frequency is analyzed using wavelet transform and different phenomenon from those obtained through FFT is observed. The time-frequency analysis further indicates that intermittence, coherence structures and self-similar structures are distinctly observed from fluctuant wind. The flow characteristics around the bridge deck at two positions are also investigated using the field measurement. The results indicate that the mean wind speed decrease when the flow passing through the deck, but the turbulence intensity become much larger and the turbulence integral lengths become much smaller compared with those of inflow. The relationship of RMS (root mean square) of wake-flow and the mean wind speed of inflow is approximately linear. The special structures of wake-flow in time-frequency domain are also analyzed using wavelet transform, which aids to reveal the forming process of wake-flow.

• Structural Engineering and Mechanics
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• v.29 no.5
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• pp.505-530
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• 2008

This paper proposes the application of active multiple tuned mass dampers (AMTMD) for translational and torsional response control of a simplified two-degree-of-freedom (2DOF) structure, able to represent the dynamic characteristics of general asymmetric structures, under the ground acceleration. This 2DOF structure is a generalized 2DOF system of an asymmetric structure with predominant translational and torsional responses under earthquake excitations using the mode reduced-order method. Depending on the ratio of the torsional to the translational eigenfrequency, i.e. the torsional to translational frequency ratio (TTFR), of asymmetric structures, the following three cases can be distinguished: (1) torsionally flexible structures (TTFR < 1.0), (2) torsionally intermediate stiff structures (TTFR = 1.0), and (3) torsionally stiff structures (TTFR > 1.0). The even distribution of the AMTMD within the whole width and half width of the asymmetric structure, thus leading to three cases of installing the AMTMD (referred to as the AMTMD of case 1, AMTMD of case 2, AMTMD of case 3, respectively), is taken into account. In the present study, the criterion for searching the optimum parameters of the AMTMD is defined as the minimization of the minimum values of the maximum translational and torsional displacement dynamic magnification factors (DMF) of an asymmetric structure with the AMTMD. The criterion used for assessing the effectiveness of the AMTMD is selected as the ratio of the minimization of the minimum values of the maximum translational and torsional displacement DMF of the asymmetric structure with the AMTMD to the maximum translational and torsional displacement DMF of the asymmetric structure without the AMTMD. By resorting to these two criteria, a careful examination of the effects of the normalized eccentricity ratio (NER) on the effectiveness and robustness of the AMTMD are carried out in the mitigation of both the translational and torsional responses of the asymmetric structure. Likewise, the effectiveness of a single ATMD with the optimum positions is presented and compared with that of the AMTMD.

• Wind and Structures
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• v.24 no.3
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• pp.205-221
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• 2017

The unsteady flow field disturbance between the blades and tower is one of the primary factors affecting the aerodynamic performance of wind turbine. Based on the research object of a 3MW horizontal axis wind turbine which was developed independently by Nanjing University of Aeronautics and Astronautics, numerical simulation on the aerodynamic performance of wind turbine system in halt state with blades in different position was conducted using large eddy simulation (LES) method. Based on the 3D unsteady numerical simulation results in a total of eight conditions (determined by the relative position with the tower during the complete rotation process of the blade), the characteristics of wind pressure distributions of the wind turbine system and action mechanism of surrounding flow field were analysed. The effect of different position of blades on the aerodynamic performance of wind turbine in halt state as well as the disturbance effect was evaluated. Results of the study showed that the halt position of blades had significant effect on the wind pressure distribution of the wind turbine system as well as the characteristics of flow around. Relevant conclusions from this study provided reference for the wind-resistant design of large scale wind turbine system in different halt states.

• Advances in robotics research
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• v.2 no.1
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• pp.45-57
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• 2018

Even though the popularity of projection mapping continues to increase and it is being implemented in more and more settings, most current projection mapping systems are limited to special purposes, such as outdoor events, live theater and musical performances. This lack of versatility arises from the large number of projectors needed and their proper calibration. Furthermore, we cannot change the positions and poses of projectors, or their projection targets, after the projectors have been calibrated. To overcome these problems, we propose a projection mapping method using a projector robot that can perform projection mapping in more general or ubiquitous situations, such as shopping malls. We can estimate a projector's position and pose with the robot's self-localization sensors, but the accuracy of this approach remains inadequate for projection mapping. Consequently, the proposed method solves this problem by combining self-localization by robot sensors with position and pose estimation of projection targets based on a 3D model. We first obtain the projection target's 3D model and then use it to accurately estimate the target's position and pose and thus achieve accurate projection mapping with a projector robot. In addition, our proposed method performs accurate projection mapping even after a projection target has been moved, which often occur in shopping malls. In this paper, we employ Ubiquitous Display (UD), which we are researching as a projector robot, to experimentally evaluate the effectiveness of the proposed method.

• Ocean Systems Engineering
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• v.7 no.2
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• pp.143-155
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• 2017

For complex flexible structures such as nets, the determination of drag forces and its deformation is a challenging task. The accurate prediction of loads on cages is one of the key steps in designing fish farm facilities. The basic physics with a simple cage, can be addressed by the use of experimental studies. However, to design more complex cage system for various environmental conditions, a reliable numerical simulation tool is essential. In this work, the current load on a cage is calculated using a Morison-force model applied at instantaneous positions of equivalent-net modeling. Variations of solidity ratio ($S_n$) of the net and current speed are considered. An equivalent array of cylinders is built to represent the physical netting. Based on the systematic comparisons between the published experimental data for Raschel nets and the current numerical simulations, carried out using the commercial software OrcaFlex, a new formulation for $C_d$ values, used in the equivalent-net model, is presented. The similar approach can also be applied to other netting materials following the same procedure. In case of high solidity ratio and current speed, the hybrid model defines $C_d$ as a function of Re (Reynolds number) and $S_n$ to better represent the corresponding weak diffraction effects. Otherwise, the conventional $C_d$ values depending only on Re can be used with including shielding effects for downstream elements. This new methodology significantly improves the agreement between numerical and experimental data.

• Geomechanics and Engineering
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• v.21 no.2
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• pp.187-200
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• 2020

In the current work, a series of three-dimensional finite element analyses have been conducted to investigate the behaviour of pre-existing single piles in response to adjacent tunnelling by considering the tunnel face pressures and the relative locations of the pile tips with respect to the tunnel. Via numerical modelling, the effect of the face pressures on the pile behaviour has been analysed. In addition, the analyses have concentrated on the ground settlements, the pile head settlements and the shear stress transfer mechanism at the pile-soil interface. The settlements of the pile directly above the tunnel crown (with a vertical distance between the pile tip and the tunnel crown of 0.25D, where D is the tunnel diameter) with a face pressure of 50% of the in situ horizontal soil stress at the tunnel springline decreased by approximately 38% compared to the corresponding pile settlements with the minimum face pressure, namely, 25% of the in situ horizontal soil stress at the tunnel springline. Furthermore, the smaller the face pressure is, the larger the tunnelling-induced ground movements, the axial pile forces and the interface shear stresses. The ground settlements and the pile settlements were heavily affected by the face pressures and the positions of the pile tip with respect to the tunnel. When the piles were inside the tunnel influence zone, tensile forces were induced on piles, while compressive pile forces were expected to develop for piles that are outside the influence zone and on the boundary. In addition, the computed results have been compared with relevant previous studies that were reported in the literature. The behaviour of the piles that is triggered by adjacent tunnelling has been extensively examined and analysed by considering the several key features in substantial detail.